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The map label for this gene is hisF

Identifier: 110680721

GI number: 110680721

Start: 3424566

End: 3425327

Strand: Direct

Name: hisF

Synonym: RD1_3561

Alternate gene names: 110680721

Gene position: 3424566-3425327 (Clockwise)

Preceding gene: 110680720

Following gene: 110680722

Centisome position: 82.86

GC content: 61.94

Gene sequence:

>762_bases
ATGTTGAAAACCCGCATCATCCCCTGTCTGGACGTCGCTGATGGCCGCGTGGTCAAAGGCGTGAACTTTGTCGATTTGCG
CGATGCGGGCGACCCGGTGGACGCCGCCATCGCCTATGATGCCGCGGGCGCGGATGAGCTGTGTTTTCTGGACATCACGG
CGACGCATGAAAACCGGGCGACCATGTTTGATGTGGTCACGCGCACGGCAGAACATTGCTATATCCCGCTGACCGTGGGC
GGGGGTGTGCGCAGCGTGTCAGATGTGCGCGCGTTGCTTTTGGCCGGTGCGGACAAGGTGTCGTTCAATTCCGCCGCCGT
GGCCAACCCCGATGTGGTCGCGCAGGCGGCGGATCAGTTTGGCAGCCAGTGCATCGTTGTGGCCATAGACGCCAAGACCA
CCTCGCCCGGCAAATGGGAAATCTTTACCCATGGCGGGCGCAAACCGACCGGCATTGACGCCGTGGCCTTCGCGCAGACA
GTGACGGCAAAGGGTGCTGGAGAAATCCTGCTTACCTCCATGGATCGTGATGGCACGCGGGCCGGGTTCAATCTGCCCCT
CACCCGCGCCATTTCAGACGCGGTGAATGTGCCCGTGATCGCCTCTGGCGGGGTCGGCACGCTCGACCACCTTGTCGAGG
GCGTAACAAAGGGCGGTGCATCCGCGGTCCTCGCCGCATCGATCTTCCATTTCGGGGATTACACGATCGCTCAGGCCAAA
CAGCACATGTCGGACGCTGGCATCCCCATGAGGCTCATATGA

Upstream 100 bases:

>100_bases
GCGCTTGAAGCGCTCAAAACGTGAGGCGCACCCCGTGATACCCGGGGGCAACAGGATCAACCCGCTTGCCCCGCCGCGCA
CGACACCCTAGAGATGCCGC

Downstream 100 bases:

>100_bases
CACTTGACGCTCTGTTCGCCACCATTCTGGACCGAAAAACTGCCGATCCCGCCAGCAGCTGGACGGCGCAACTTCTCTCC
AAAGGACCGGAAAAATGCGC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MLKTRIIPCLDVADGRVVKGVNFVDLRDAGDPVDAAIAYDAAGADELCFLDITATHENRATMFDVVTRTAEHCYIPLTVG
GGVRSVSDVRALLLAGADKVSFNSAAVANPDVVAQAADQFGSQCIVVAIDAKTTSPGKWEIFTHGGRKPTGIDAVAFAQT
VTAKGAGEILLTSMDRDGTRAGFNLPLTRAISDAVNVPVIASGGVGTLDHLVEGVTKGGASAVLAASIFHFGDYTIAQAK
QHMSDAGIPMRLI

Sequences:

>Translated_253_residues
MLKTRIIPCLDVADGRVVKGVNFVDLRDAGDPVDAAIAYDAAGADELCFLDITATHENRATMFDVVTRTAEHCYIPLTVG
GGVRSVSDVRALLLAGADKVSFNSAAVANPDVVAQAADQFGSQCIVVAIDAKTTSPGKWEIFTHGGRKPTGIDAVAFAQT
VTAKGAGEILLTSMDRDGTRAGFNLPLTRAISDAVNVPVIASGGVGTLDHLVEGVTKGGASAVLAASIFHFGDYTIAQAK
QHMSDAGIPMRLI
>Mature_253_residues
MLKTRIIPCLDVADGRVVKGVNFVDLRDAGDPVDAAIAYDAAGADELCFLDITATHENRATMFDVVTRTAEHCYIPLTVG
GGVRSVSDVRALLLAGADKVSFNSAAVANPDVVAQAADQFGSQCIVVAIDAKTTSPGKWEIFTHGGRKPTGIDAVAFAQT
VTAKGAGEILLTSMDRDGTRAGFNLPLTRAISDAVNVPVIASGGVGTLDHLVEGVTKGGASAVLAASIFHFGDYTIAQAK
QHMSDAGIPMRLI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=41.2451361867704, Blast_Score=196, Evalue=9e-52,
Organism=Escherichia coli, GI87082028, Length=238, Percent_Identity=31.0924369747899, Blast_Score=102, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=30.2547770700637, Blast_Score=139, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_ROSDO (Q162Q1)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_683728.1
- ProteinModelPortal:   Q162Q1
- SMR:   Q162Q1
- STRING:   Q162Q1
- GeneID:   4195792
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_3561
- NMPDR:   fig|375451.6.peg.3310
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- BioCyc:   RDEN375451:RD1_3561-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 26223; Mature: 26223

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKTRIIPCLDVADGRVVKGVNFVDLRDAGDPVDAAIAYDAAGADELCFLDITATHENRA
CCCCCEEEEEECCCCCEECCCCEEEECCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCH
TMFDVVTRTAEHCYIPLTVGGGVRSVSDVRALLLAGADKVSFNSAAVANPDVVAQAADQF
HHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHC
GSQCIVVAIDAKTTSPGKWEIFTHGGRKPTGIDAVAFAQTVTAKGAGEILLTSMDRDGTR
CCEEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCC
AGFNLPLTRAISDAVNVPVIASGGVGTLDHLVEGVTKGGASAVLAASIFHFGDYTIAQAK
CCCCCCHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHH
QHMSDAGIPMRLI
HHHHHCCCCEEEC
>Mature Secondary Structure
MLKTRIIPCLDVADGRVVKGVNFVDLRDAGDPVDAAIAYDAAGADELCFLDITATHENRA
CCCCCEEEEEECCCCCEECCCCEEEECCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCH
TMFDVVTRTAEHCYIPLTVGGGVRSVSDVRALLLAGADKVSFNSAAVANPDVVAQAADQF
HHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHC
GSQCIVVAIDAKTTSPGKWEIFTHGGRKPTGIDAVAFAQTVTAKGAGEILLTSMDRDGTR
CCEEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCC
AGFNLPLTRAISDAVNVPVIASGGVGTLDHLVEGVTKGGASAVLAASIFHFGDYTIAQAK
CCCCCCHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHH
QHMSDAGIPMRLI
HHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA