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The map label for this gene is hisH [H]

Identifier: 110680717

GI number: 110680717

Start: 3421684

End: 3422322

Strand: Direct

Name: hisH [H]

Synonym: RD1_3557

Alternate gene names: 110680717

Gene position: 3421684-3422322 (Clockwise)

Preceding gene: 110680716

Following gene: 110680720

Centisome position: 82.79

GC content: 59.0

Gene sequence:

>639_bases
GTGCTGACGGCGATTATCGATTACGAAAGCGGAAACCTGCATTCCGCGCATAAAGCCTTTGAACGCATGGCAAATGAAAC
CGGGGGCGGCGACGTGGTTGTGACGGGCGATGCGGATGTGGTGGCGCGTGCCGATCGCATCGTGCTGCCCGGCGACGGGG
CGTTTCCCGCCTGCATGGCCGCGCTCAAAGGGCATTCCGGGCTCTATGCTGCGATGATCGAAGCCGTGGAGGGCAAAGGC
CGCCCCTTCATGGGGATTTGCGTTGGCATGCAGCTGATGGCGACGCTGGGCAGGGAATACCGCGACACGGAAGGACTGGA
TTGGATCAGTGGCGAAGTGATCAGGATCACGCCGCATGATTCGTCACTGAAAGTCCCCCACATGGGCTGGAACGATCTGA
TCATTGATGCCCCGCACCCTGTCTTTGAAGGGATCACCACCGGGGATCACGCCTATTTCGTCCATAGCTATCATATGGAT
GTGACACATGACGCAGAACGGCTGGCGCATGTGGATTATTCAGGCGATGTGACGGCCGTGATCGGGCGGGACAATATGAT
CGGCCTGCAGTTTCACCCGGAAAAGAGCCAGTCTGCCGGGCTGCGCATGATTGCCAACTTTCTTGCATGGCGGCCCTGA

Upstream 100 bases:

>100_bases
AGGCCACGTTCAAATCCGTCGCCCGTGCCCTGCGTCAAGCGGTTGAAACGGATCCGCGTAAGTCTGACGCGATCCCGTCC
ACCAAAGGCGCACTCTGAGC

Downstream 100 bases:

>100_bases
GGGGCTCGGCAGCGCCCAAGCGTCAGTTGTCTCTGCTCATGGACTTCAAAAGCTCCTGCATGGGCGTCTGGTCGCTCAAC
AGGAAACCGTGCCTTGGATC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MLTAIIDYESGNLHSAHKAFERMANETGGGDVVVTGDADVVARADRIVLPGDGAFPACMAALKGHSGLYAAMIEAVEGKG
RPFMGICVGMQLMATLGREYRDTEGLDWISGEVIRITPHDSSLKVPHMGWNDLIIDAPHPVFEGITTGDHAYFVHSYHMD
VTHDAERLAHVDYSGDVTAVIGRDNMIGLQFHPEKSQSAGLRMIANFLAWRP

Sequences:

>Translated_212_residues
MLTAIIDYESGNLHSAHKAFERMANETGGGDVVVTGDADVVARADRIVLPGDGAFPACMAALKGHSGLYAAMIEAVEGKG
RPFMGICVGMQLMATLGREYRDTEGLDWISGEVIRITPHDSSLKVPHMGWNDLIIDAPHPVFEGITTGDHAYFVHSYHMD
VTHDAERLAHVDYSGDVTAVIGRDNMIGLQFHPEKSQSAGLRMIANFLAWRP
>Mature_212_residues
MLTAIIDYESGNLHSAHKAFERMANETGGGDVVVTGDADVVARADRIVLPGDGAFPACMAALKGHSGLYAAMIEAVEGKG
RPFMGICVGMQLMATLGREYRDTEGLDWISGEVIRITPHDSSLKVPHMGWNDLIIDAPHPVFEGITTGDHAYFVHSYHMD
VTHDAERLAHVDYSGDVTAVIGRDNMIGLQFHPEKSQSAGLRMIANFLAWRP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=211, Percent_Identity=36.4928909952607, Blast_Score=125, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6319725, Length=216, Percent_Identity=32.4074074074074, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 23029; Mature: 23029

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTAIIDYESGNLHSAHKAFERMANETGGGDVVVTGDADVVARADRIVLPGDGAFPACMA
CEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEECCCHHEECCCEEEECCCCCHHHHHH
ALKGHSGLYAAMIEAVEGKGRPFMGICVGMQLMATLGREYRDTEGLDWISGEVIRITPHD
HHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHCCCCCCCCCCCCEEEEECCC
SSLKVPHMGWNDLIIDAPHPVFEGITTGDHAYFVHSYHMDVTHDAERLAHVDYSGDVTAV
CCEECCCCCCCCEEEECCCHHHCCCCCCCEEEEEEEEECCCCCCHHHHEECCCCCCEEEE
IGRDNMIGLQFHPEKSQSAGLRMIANFLAWRP
ECCCCEEEEEECCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLTAIIDYESGNLHSAHKAFERMANETGGGDVVVTGDADVVARADRIVLPGDGAFPACMA
CEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEECCCHHEECCCEEEECCCCCHHHHHH
ALKGHSGLYAAMIEAVEGKGRPFMGICVGMQLMATLGREYRDTEGLDWISGEVIRITPHD
HHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHCCCCCCCCCCCCEEEEECCC
SSLKVPHMGWNDLIIDAPHPVFEGITTGDHAYFVHSYHMDVTHDAERLAHVDYSGDVTAV
CCEECCCCCCCCEEEECCCHHHCCCCCCCEEEEEEEEECCCCCCHHHHEECCCCCCEEEE
IGRDNMIGLQFHPEKSQSAGLRMIANFLAWRP
ECCCCEEEEEECCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA