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The map label for this gene is ybfF [H]

Identifier: 110680652

GI number: 110680652

Start: 3355998

End: 3356759

Strand: Direct

Name: ybfF [H]

Synonym: RD1_3489

Alternate gene names: 110680652

Gene position: 3355998-3356759 (Clockwise)

Preceding gene: 110680648

Following gene: 110680653

Centisome position: 81.2

GC content: 58.92

Gene sequence:

>762_bases
TTGGCCATGTTGAACGTGAATGAATTTGGCGCGCCCACCGCCCCCGCCGTGATGATTGTCCATGGGCTTTACGGCTCTGC
GCGCAACTGGGGCGTGATTGCCAAACGCCTGTCTGATGGCTTTCGCGTCTATACGGTCGATCTGCGCAACCACGGTTTGA
GCCCCCACACCCAAACGCACAGCTATCCTGAAATGGCCGCCGACCTGGCCGAAACGATCGAACATCTGGGCGGCCCGGTG
CAGCTTGTTGGCCATTCCATGGGGGGCAAAGCAGCGATGGCTCTCGCGCTGACCCGCCCTGACCTCGTGCACCGCCTGCT
CGTGGCCGACATCGCGCCGGTGGCATATACGCACAGCCAGCTTCCGTTCATCCACGCCATGAAAGCTGTGAATCTGGACG
CCGTGTCACGTCGTTCGGATGCAGAGGCGCAATTGGCAGCCCAAGGTGTCGAACCTGCACTGTGCAGCTTTTTCACGCAG
TCGCTTGATCTGGGCGAAAAACGCTGGCGGTTGAATCTTGCAACGCTGGAAGCTGAAATGGATAAAATCATGTCCTTTCC
GCAGTTCGACACGCGCTTTGACGGCCCCACGCTGTTCTTGAGCGGTGCAGAGTCCGACTACGTTCAACCGCAGCATCGAC
AAACCATAAAATCGCTGTTTACGCGGGCGCAATTTGCGAAACTGCGCGGCGCAGGCCACTGGCTGCACGCCGACAAACCC
CGCGAATTCGAAACGGCAGTGCGTAGCTTTTTCGCAAGCTGA

Upstream 100 bases:

>100_bases
CTTTTGCGGTGATCTTCTCGCTCTCCTGCATGGCGCTGCTCCCTGTCAGAACGCTTCTCGCCGAATGGTTCTGGCAGAGC
CTAGCGAAATCCGCTAGCCC

Downstream 100 bases:

>100_bases
GGTACAATCCCCCTTTCCCCTTGCACGTCAGCCCGGCTTGCCTATAACGCAGGACAATTTGGACGCATATGGCGTTTTGA
AAATTGGGGGCCTGATCATG

Product: hydrolase, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MAMLNVNEFGAPTAPAVMIVHGLYGSARNWGVIAKRLSDGFRVYTVDLRNHGLSPHTQTHSYPEMAADLAETIEHLGGPV
QLVGHSMGGKAAMALALTRPDLVHRLLVADIAPVAYTHSQLPFIHAMKAVNLDAVSRRSDAEAQLAAQGVEPALCSFFTQ
SLDLGEKRWRLNLATLEAEMDKIMSFPQFDTRFDGPTLFLSGAESDYVQPQHRQTIKSLFTRAQFAKLRGAGHWLHADKP
REFETAVRSFFAS

Sequences:

>Translated_253_residues
MAMLNVNEFGAPTAPAVMIVHGLYGSARNWGVIAKRLSDGFRVYTVDLRNHGLSPHTQTHSYPEMAADLAETIEHLGGPV
QLVGHSMGGKAAMALALTRPDLVHRLLVADIAPVAYTHSQLPFIHAMKAVNLDAVSRRSDAEAQLAAQGVEPALCSFFTQ
SLDLGEKRWRLNLATLEAEMDKIMSFPQFDTRFDGPTLFLSGAESDYVQPQHRQTIKSLFTRAQFAKLRGAGHWLHADKP
REFETAVRSFFAS
>Mature_252_residues
AMLNVNEFGAPTAPAVMIVHGLYGSARNWGVIAKRLSDGFRVYTVDLRNHGLSPHTQTHSYPEMAADLAETIEHLGGPVQ
LVGHSMGGKAAMALALTRPDLVHRLLVADIAPVAYTHSQLPFIHAMKAVNLDAVSRRSDAEAQLAAQGVEPALCSFFTQS
LDLGEKRWRLNLATLEAEMDKIMSFPQFDTRFDGPTLFLSGAESDYVQPQHRQTIKSLFTRAQFAKLRGAGHWLHADKPR
EFETAVRSFFAS

Specific function: Displays esterase activity toward palmitoyl-CoA, malonyl-CoA and pNP-butyrate [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI23200012, Length=253, Percent_Identity=44.2687747035573, Blast_Score=190, Evalue=1e-48,
Organism=Homo sapiens, GI23200008, Length=253, Percent_Identity=44.2687747035573, Blast_Score=190, Evalue=1e-48,
Organism=Homo sapiens, GI223941839, Length=246, Percent_Identity=38.2113821138211, Blast_Score=150, Evalue=1e-36,
Organism=Escherichia coli, GI1786902, Length=239, Percent_Identity=35.9832635983264, Blast_Score=145, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17508535, Length=250, Percent_Identity=37.6, Blast_Score=170, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17507063, Length=261, Percent_Identity=31.4176245210728, Blast_Score=116, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6321468, Length=270, Percent_Identity=32.5925925925926, Blast_Score=105, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6321452, Length=291, Percent_Identity=21.9931271477663, Blast_Score=68, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24640348, Length=255, Percent_Identity=33.3333333333333, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24646055, Length=104, Percent_Identity=47.1153846153846, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 27867; Mature: 27736

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMLNVNEFGAPTAPAVMIVHGLYGSARNWGVIAKRLSDGFRVYTVDLRNHGLSPHTQTH
CCEECCHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCC
SYPEMAADLAETIEHLGGPVQLVGHSMGGKAAMALALTRPDLVHRLLVADIAPVAYTHSQ
CCHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCC
LPFIHAMKAVNLDAVSRRSDAEAQLAAQGVEPALCSFFTQSLDLGEKRWRLNLATLEAEM
CHHHHHHHHCCHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCHHHEEHHHHHHHHH
DKIMSFPQFDTRFDGPTLFLSGAESDYVQPQHRQTIKSLFTRAQFAKLRGAGHWLHADKP
HHHHCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
REFETAVRSFFAS
HHHHHHHHHHHCC
>Mature Secondary Structure 
AMLNVNEFGAPTAPAVMIVHGLYGSARNWGVIAKRLSDGFRVYTVDLRNHGLSPHTQTH
CEECCHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCC
SYPEMAADLAETIEHLGGPVQLVGHSMGGKAAMALALTRPDLVHRLLVADIAPVAYTHSQ
CCHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCC
LPFIHAMKAVNLDAVSRRSDAEAQLAAQGVEPALCSFFTQSLDLGEKRWRLNLATLEAEM
CHHHHHHHHCCHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCHHHEEHHHHHHHHH
DKIMSFPQFDTRFDGPTLFLSGAESDYVQPQHRQTIKSLFTRAQFAKLRGAGHWLHADKP
HHHHCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
REFETAVRSFFAS
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]