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The map label for this gene is yfgF [H]

Identifier: 110680564

GI number: 110680564

Start: 3260054

End: 3260926

Strand: Direct

Name: yfgF [H]

Synonym: RD1_3393

Alternate gene names: 110680564

Gene position: 3260054-3260926 (Clockwise)

Preceding gene: 110680561

Following gene: 110680565

Centisome position: 78.88

GC content: 55.9

Gene sequence:

>873_bases
ATGGCACAAACCAAGAACGTTAGGAACATCATGCCTGCTTCAATCCAACGCGTCGTGGCCGACGTCGCTCCGCAGAGTGA
CAACCCTTTGAACTTCGCTCTTACAGCACGGGACCAGAAGACCATCGATATGGTGGCGACAGCGGTCAAAAACAAGCAGG
TGTTGCTGGCCTATCAGCCGATCATGCAGGCACGGGATCAAAACAAAGTTGCCTTTTACGAGGGCTTGATCCGCGTTCTT
GACGAAACAGGCCGCATTATTCCCGCAGGCGCGTTCATGGATGCGATCGAAAACATCGAACTTGGGCGGCAGTTGGACAC
GCTGGCCCTGGATGAGGGTCTGCGCGCCTTGCACGAAAACCCGGAGTTACGCTTGTCAATCAACATGTCAGCACGCTCCA
TTGGGTATGCGCCGTGGAATCGCATGCTCGACGAATGGCTCACGCGGAATGAACTGATCGGAGAGCGGTTGATTCTTGAG
ATCACAGAAAGCAGCGCCCTGGTCGCGCCTGAACTGGTCGTGGACTTCATGGACCGCCTTCAACTGAGGGGTATCTGCTT
TGCCATGGATGACTTTGGCGCAGGTTACACCGCTCTGCGGTACTTCAAGGAGTTCTGTTTTGACGTGATCAAGATTGACG
GGCAGTTCATCAAGGGCATCGCGGACGATCCCGACAATCAGGCGATGACCGCCGCTCTGGTGTCTATCGCCAAACACTTC
GACATGCTGACGGTTGCGGAATTCGTGGAATGCGAAGCGGATGCGCAAAAACTCGTTAGCCTCGGGGTCGACTGCCTGCA
AGGGTATCATTTTGCGGCCGCCACGACCCGCCCCGCTTGGCTGCGCGACACACACCTCAGTCAGGCCGTTTAA

Upstream 100 bases:

>100_bases
TATTATCTTTTCTTTTCAGTGTTTTCAGCTTCTTTAAACCACATATAAAATTGGAACAAAATAAGAATGTTCACCGAGTT
TTAACATCTGTGGTGACATT

Downstream 100 bases:

>100_bases
CGATTCAACTTCTTTCATGTTTGCGGTGCTGTGTCACAGCTTTGAGATGTTGCCCGGTTCTTAATGTTACGTTAACAGTT
GCCCACATCGCAGAAAGGGC

Product: EAL domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MAQTKNVRNIMPASIQRVVADVAPQSDNPLNFALTARDQKTIDMVATAVKNKQVLLAYQPIMQARDQNKVAFYEGLIRVL
DETGRIIPAGAFMDAIENIELGRQLDTLALDEGLRALHENPELRLSINMSARSIGYAPWNRMLDEWLTRNELIGERLILE
ITESSALVAPELVVDFMDRLQLRGICFAMDDFGAGYTALRYFKEFCFDVIKIDGQFIKGIADDPDNQAMTAALVSIAKHF
DMLTVAEFVECEADAQKLVSLGVDCLQGYHFAAATTRPAWLRDTHLSQAV

Sequences:

>Translated_290_residues
MAQTKNVRNIMPASIQRVVADVAPQSDNPLNFALTARDQKTIDMVATAVKNKQVLLAYQPIMQARDQNKVAFYEGLIRVL
DETGRIIPAGAFMDAIENIELGRQLDTLALDEGLRALHENPELRLSINMSARSIGYAPWNRMLDEWLTRNELIGERLILE
ITESSALVAPELVVDFMDRLQLRGICFAMDDFGAGYTALRYFKEFCFDVIKIDGQFIKGIADDPDNQAMTAALVSIAKHF
DMLTVAEFVECEADAQKLVSLGVDCLQGYHFAAATTRPAWLRDTHLSQAV
>Mature_289_residues
AQTKNVRNIMPASIQRVVADVAPQSDNPLNFALTARDQKTIDMVATAVKNKQVLLAYQPIMQARDQNKVAFYEGLIRVLD
ETGRIIPAGAFMDAIENIELGRQLDTLALDEGLRALHENPELRLSINMSARSIGYAPWNRMLDEWLTRNELIGERLILEI
TESSALVAPELVVDFMDRLQLRGICFAMDDFGAGYTALRYFKEFCFDVIKIDGQFIKGIADDPDNQAMTAALVSIAKHFD
MLTVAEFVECEADAQKLVSLGVDCLQGYHFAAATTRPAWLRDTHLSQAV

Specific function: Truncated proteins consisting of the GGDEF/EAL domains (residues 319-747) or of the EAL domain alone (481-747) have c-di- GMP phosphodiesterase activity. They do not have diguanylate cyclase activity. Cyclic-di-GMP is a second messenger which controls cel

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1788849, Length=226, Percent_Identity=30.5309734513274, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI226510982, Length=235, Percent_Identity=31.9148936170213, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788502, Length=256, Percent_Identity=28.90625, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87082096, Length=239, Percent_Identity=28.0334728033473, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787541, Length=229, Percent_Identity=27.0742358078603, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081845, Length=242, Percent_Identity=28.099173553719, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081980, Length=231, Percent_Identity=29.4372294372294, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1786507, Length=241, Percent_Identity=29.8755186721992, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI87081743, Length=250, Percent_Identity=23.2, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1790496, Length=231, Percent_Identity=25.5411255411255, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787055, Length=252, Percent_Identity=27.7777777777778, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87081921, Length=245, Percent_Identity=26.530612244898, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR007895 [H]

Pfam domain/function: PF00563 EAL; PF05231 MASE1 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 32370; Mature: 32238

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQTKNVRNIMPASIQRVVADVAPQSDNPLNFALTARDQKTIDMVATAVKNKQVLLAYQP
CCCCCCHHHCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEEHH
IMQARDQNKVAFYEGLIRVLDETGRIIPAGAFMDAIENIELGRQLDTLALDEGLRALHEN
HHHHCCCCCHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
PELRLSINMSARSIGYAPWNRMLDEWLTRNELIGERLILEITESSALVAPELVVDFMDRL
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHH
QLRGICFAMDDFGAGYTALRYFKEFCFDVIKIDGQFIKGIADDPDNQAMTAALVSIAKHF
HHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHH
DMLTVAEFVECEADAQKLVSLGVDCLQGYHFAAATTRPAWLRDTHLSQAV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
AQTKNVRNIMPASIQRVVADVAPQSDNPLNFALTARDQKTIDMVATAVKNKQVLLAYQP
CCCCCHHHCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEEHH
IMQARDQNKVAFYEGLIRVLDETGRIIPAGAFMDAIENIELGRQLDTLALDEGLRALHEN
HHHHCCCCCHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
PELRLSINMSARSIGYAPWNRMLDEWLTRNELIGERLILEITESSALVAPELVVDFMDRL
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHH
QLRGICFAMDDFGAGYTALRYFKEFCFDVIKIDGQFIKGIADDPDNQAMTAALVSIAKHF
HHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHH
DMLTVAEFVECEADAQKLVSLGVDCLQGYHFAAATTRPAWLRDTHLSQAV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]