| Definition | Roseobacter denitrificans OCh 114, complete genome. |
|---|---|
| Accession | NC_008209 |
| Length | 4,133,097 |
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The map label for this gene is cobT [H]
Identifier: 110680518
GI number: 110680518
Start: 3204283
End: 3206160
Strand: Direct
Name: cobT [H]
Synonym: RD1_3340
Alternate gene names: 110680518
Gene position: 3204283-3206160 (Clockwise)
Preceding gene: 110680517
Following gene: 110680519
Centisome position: 77.53
GC content: 63.42
Gene sequence:
>1878_bases ATGAGCAAGCCTTCTGACAATCCCGCAGATGCCTTTAAAAAGGCGCTGACCGAGGCGACGAAGGTCATGGCGGATGATCC GGAGCTGTCGGTCAGCTATTCGGTGGATCCGGCGGGCCTGTCCGGGGATGCGATGCGTTTGCCGCAGGTGAGCCGCCGCA TGACGCGCGCCGAGGTTCTGTTGGCGCGCGGCACGGCGGATGCTCTGGCGCTGAACCGGCGCTATCACAATGGTGAGACC CACGCCCGCTATGCCCCACAAGGCGAAATGGCGCGCGAGTTGTACGAGGCGATGGAGACCGCCCGCTGCGAGGCCGTGGG CGCGCGCGACATGCCGGGTACGGCGGGCAATATTGACGCCAAGATCGCGCATGAAGCGATGCGCAAGGGCTATGATCAGG TCACGCAGGCGGCGGATGTACCGCTGGCCACGGCGGCAGGCTATCTGATCCGCCATCTTGCGACGGGCCGCGATCTGCCT TCCGGGGCGCAAAACGTCATGGATCTGTGGCGCGGTTTCATCGAAGAGCAGGCCGGCGGTACGCTTGACGCATTGGATGA GACGTTGGCGGATCAGGCGGCGTTTGCGAAATTCTCCCGTCAGATGATCTCCGATCTGGGCTATGGGGACCAGCTTGGCG ATGATCCCGATCAGGTGGACGAGGATCAGGAAGACGACGCCGAAGAGGGCAGCGAGGATCAGAACGAACCCGACAGCACC GGGCAGGATGATCAGGACGAGGAAGACGCGGACGCCTCCCCCGAACAGAGCCAGGAAGATCAGCAGGATGCCGCGCAGGC GCAGGTCTCCATGGATGACATGGCCGATCAGGAGATGGGCGATGAAGCGGAAATGCCCGAAGGCGAGGCCCCGCTTGAGC CACCCGCCCCGCAGCCGGTGTCGGATGCGGACCCGAATTACATGGTCTACCTCGGGGATCACGACGAAGAGATCAACGCC GAAGACCTCGCCGAACCGGTTGAGCTGGAACGTCTGCGCGCCTATCTCGATCAGCAGCTTGAGCCGCTCAAAGGGGCCGT GAGCCGCCTTGCGAACAAATTGCAACGACGCCTTCAGGCGCAGCAGAACCGGTCATGGGAATTCGACCTCGAAGAGGGCA TGCTTGATGCGGGCCGGCTGGCGCGTGTGGTGGCCAACCCGACCACGCCGCTCAGCTTCAAGGTCGAGAAGGACACCGAG TTTCGCGATACCGTGGTGACGCTGCTGCTGGACAACTCCGGCTCCATGCGGGGGCGTCCGATTTCGATTGCGGCGATTTG TGCCGATGTGCTGGCGCGCACGCTGGAGCGCTGCAACGTCAAGGTGGAAATCCTCGGCTTTACCACCCGCGCGTGGAAAG GCGGGCAGGCCCGCGAGGCCTGGCTGAACGATGGCCGCCCCCAACAGCCCGGCCGGCTGAACGACCTGCGCCACATCATC TACAAATCGGCCGATGCGCCCTGGCGGCGGACCCGTCCCAATCTGGGCCTGATGATGAAAGAAGGGTTGCTCAAGGAAAA CATCGACGGGGAAGCGCTTGAATGGGCGCACCGGCGTATGCTGATGCGGGGCGAGGCGCGCAAGATCCTCATGGTCATCT CGGATGGTGCGCCGGTGGATGACAGCACGCTGTCCGTGAACCCCGCGAACTATCTGGAAAAGCACCTGCGCGATGTGATC GCCTTGGTCGAAAAGCGCAAAGCGGTTGAACTGTTGGCAATCGGGATCGGTCACGATGTCACGCGCTATTATGAGCGGGC GGTGACCATTACGGATGTCGACCAGCTTGCAGGCGCGATGACCGAACAACTGGCCGCCCTTTTTGACAGCGACCCGCGCG CACGCGCGCGCGTCATGGGCATGCGCCGCGCAGGCTAA
Upstream 100 bases:
>100_bases GTTCGACGAGGAACTGCCCGAAAGCGCGGCAAGCCTGAGCTTGGGGTAAGGTGCTGAACGTGGCGCGGAGACCCCGTGCC AATCCGATGGGAACAACCGT
Downstream 100 bases:
>100_bases ACCCGGCTCCAGTTCTATCGCGGCACAATCAAAGGGATCCATCGCTATGTTTCAGTCATTTGAAGTCACAGCCCGCCCGG AACAGGGCCCCCCGCGTCTC
Product: aerobic cobaltochelatase CobT subunit
Products: NA
Alternate protein names: Hydrogenobyrinic acid a,c-diamide cobaltochelatase subunit CobT [H]
Number of amino acids: Translated: 625; Mature: 624
Protein sequence:
>625_residues MSKPSDNPADAFKKALTEATKVMADDPELSVSYSVDPAGLSGDAMRLPQVSRRMTRAEVLLARGTADALALNRRYHNGET HARYAPQGEMARELYEAMETARCEAVGARDMPGTAGNIDAKIAHEAMRKGYDQVTQAADVPLATAAGYLIRHLATGRDLP SGAQNVMDLWRGFIEEQAGGTLDALDETLADQAAFAKFSRQMISDLGYGDQLGDDPDQVDEDQEDDAEEGSEDQNEPDST GQDDQDEEDADASPEQSQEDQQDAAQAQVSMDDMADQEMGDEAEMPEGEAPLEPPAPQPVSDADPNYMVYLGDHDEEINA EDLAEPVELERLRAYLDQQLEPLKGAVSRLANKLQRRLQAQQNRSWEFDLEEGMLDAGRLARVVANPTTPLSFKVEKDTE FRDTVVTLLLDNSGSMRGRPISIAAICADVLARTLERCNVKVEILGFTTRAWKGGQAREAWLNDGRPQQPGRLNDLRHII YKSADAPWRRTRPNLGLMMKEGLLKENIDGEALEWAHRRMLMRGEARKILMVISDGAPVDDSTLSVNPANYLEKHLRDVI ALVEKRKAVELLAIGIGHDVTRYYERAVTITDVDQLAGAMTEQLAALFDSDPRARARVMGMRRAG
Sequences:
>Translated_625_residues MSKPSDNPADAFKKALTEATKVMADDPELSVSYSVDPAGLSGDAMRLPQVSRRMTRAEVLLARGTADALALNRRYHNGET HARYAPQGEMARELYEAMETARCEAVGARDMPGTAGNIDAKIAHEAMRKGYDQVTQAADVPLATAAGYLIRHLATGRDLP SGAQNVMDLWRGFIEEQAGGTLDALDETLADQAAFAKFSRQMISDLGYGDQLGDDPDQVDEDQEDDAEEGSEDQNEPDST GQDDQDEEDADASPEQSQEDQQDAAQAQVSMDDMADQEMGDEAEMPEGEAPLEPPAPQPVSDADPNYMVYLGDHDEEINA EDLAEPVELERLRAYLDQQLEPLKGAVSRLANKLQRRLQAQQNRSWEFDLEEGMLDAGRLARVVANPTTPLSFKVEKDTE FRDTVVTLLLDNSGSMRGRPISIAAICADVLARTLERCNVKVEILGFTTRAWKGGQAREAWLNDGRPQQPGRLNDLRHII YKSADAPWRRTRPNLGLMMKEGLLKENIDGEALEWAHRRMLMRGEARKILMVISDGAPVDDSTLSVNPANYLEKHLRDVI ALVEKRKAVELLAIGIGHDVTRYYERAVTITDVDQLAGAMTEQLAALFDSDPRARARVMGMRRAG >Mature_624_residues SKPSDNPADAFKKALTEATKVMADDPELSVSYSVDPAGLSGDAMRLPQVSRRMTRAEVLLARGTADALALNRRYHNGETH ARYAPQGEMARELYEAMETARCEAVGARDMPGTAGNIDAKIAHEAMRKGYDQVTQAADVPLATAAGYLIRHLATGRDLPS GAQNVMDLWRGFIEEQAGGTLDALDETLADQAAFAKFSRQMISDLGYGDQLGDDPDQVDEDQEDDAEEGSEDQNEPDSTG QDDQDEEDADASPEQSQEDQQDAAQAQVSMDDMADQEMGDEAEMPEGEAPLEPPAPQPVSDADPNYMVYLGDHDEEINAE DLAEPVELERLRAYLDQQLEPLKGAVSRLANKLQRRLQAQQNRSWEFDLEEGMLDAGRLARVVANPTTPLSFKVEKDTEF RDTVVTLLLDNSGSMRGRPISIAAICADVLARTLERCNVKVEILGFTTRAWKGGQAREAWLNDGRPQQPGRLNDLRHIIY KSADAPWRRTRPNLGLMMKEGLLKENIDGEALEWAHRRMLMRGEARKILMVISDGAPVDDSTLSVNPANYLEKHLRDVIA LVEKRKAVELLAIGIGHDVTRYYERAVTITDVDQLAGAMTEQLAALFDSDPRARARVMGMRRAG
Specific function: Catalyzes cobalt insertion in the corrin ring [H]
COG id: COG4547
COG function: function code H; Cobalamin biosynthesis protein CobT (nicotinate-mononucleotide:5, 6-dimethylbenzimidazole phosphoribosyltransferase)
Gene ontology:
Cell location: Cytoplasm (Potential) [H]
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Contains 1 VWFA domain [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006538 - InterPro: IPR002035 [H]
Pfam domain/function: PF06213 CobT [H]
EC number: =6.6.1.2 [H]
Molecular weight: Translated: 68961; Mature: 68829
Theoretical pI: Translated: 4.32; Mature: 4.32
Prosite motif: PS50234 VWFA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.5 %Cys (Translated Protein) 4.0 %Met (Translated Protein) 4.5 %Cys+Met (Translated Protein) 0.5 %Cys (Mature Protein) 3.8 %Met (Mature Protein) 4.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSKPSDNPADAFKKALTEATKVMADDPELSVSYSVDPAGLSGDAMRLPQVSRRMTRAEVL CCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHCHHHHHHHHHHHHH LARGTADALALNRRYHNGETHARYAPQGEMARELYEAMETARCEAVGARDMPGTAGNIDA HHCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH KIAHEAMRKGYDQVTQAADVPLATAAGYLIRHLATGRDLPSGAQNVMDLWRGFIEEQAGG HHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCC TLDALDETLADQAAFAKFSRQMISDLGYGDQLGDDPDQVDEDQEDDAEEGSEDQNEPDST CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCHHCCCCCCCCCCCC GQDDQDEEDADASPEQSQEDQQDAAQAQVSMDDMADQEMGDEAEMPEGEAPLEPPAPQPV CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC SDADPNYMVYLGDHDEEINAEDLAEPVELERLRAYLDQQLEPLKGAVSRLANKLQRRLQA CCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH QQNRSWEFDLEEGMLDAGRLARVVANPTTPLSFKVEKDTEFRDTVVTLLLDNSGSMRGRP HHCCCCCCCHHHCCCCHHHHHHHHCCCCCCCEEEECCCCCHHHHEEEEEECCCCCCCCCC ISIAAICADVLARTLERCNVKVEILGFTTRAWKGGQAREAWLNDGRPQQPGRLNDLRHII HHHHHHHHHHHHHHHHHCCCEEEEEEEEHHCCCCCCHHHHHHCCCCCCCCCHHHHHHHHH YKSADAPWRRTRPNLGLMMKEGLLKENIDGEALEWAHRRMLMRGEARKILMVISDGAPVD HHCCCCCHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCC DSTLSVNPANYLEKHLRDVIALVEKRKAVELLAIGIGHDVTRYYERAVTITDVDQLAGAM CCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHCEEECHHHHHHHH TEQLAALFDSDPRARARVMGMRRAG HHHHHHHHCCCHHHHHHHHHHHCCC >Mature Secondary Structure SKPSDNPADAFKKALTEATKVMADDPELSVSYSVDPAGLSGDAMRLPQVSRRMTRAEVL CCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHCHHHHHHHHHHHHH LARGTADALALNRRYHNGETHARYAPQGEMARELYEAMETARCEAVGARDMPGTAGNIDA HHCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH KIAHEAMRKGYDQVTQAADVPLATAAGYLIRHLATGRDLPSGAQNVMDLWRGFIEEQAGG HHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCC TLDALDETLADQAAFAKFSRQMISDLGYGDQLGDDPDQVDEDQEDDAEEGSEDQNEPDST CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCHHCCCCCCCCCCCC GQDDQDEEDADASPEQSQEDQQDAAQAQVSMDDMADQEMGDEAEMPEGEAPLEPPAPQPV CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC SDADPNYMVYLGDHDEEINAEDLAEPVELERLRAYLDQQLEPLKGAVSRLANKLQRRLQA CCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH QQNRSWEFDLEEGMLDAGRLARVVANPTTPLSFKVEKDTEFRDTVVTLLLDNSGSMRGRP HHCCCCCCCHHHCCCCHHHHHHHHCCCCCCCEEEECCCCCHHHHEEEEEECCCCCCCCCC ISIAAICADVLARTLERCNVKVEILGFTTRAWKGGQAREAWLNDGRPQQPGRLNDLRHII HHHHHHHHHHHHHHHHHCCCEEEEEEEEHHCCCCCCHHHHHHCCCCCCCCCHHHHHHHHH YKSADAPWRRTRPNLGLMMKEGLLKENIDGEALEWAHRRMLMRGEARKILMVISDGAPVD HHCCCCCHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCC DSTLSVNPANYLEKHLRDVIALVEKRKAVELLAIGIGHDVTRYYERAVTITDVDQLAGAM CCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHCEEECHHHHHHHH TEQLAALFDSDPRARARVMGMRRAG HHHHHHHHCCCHHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 1917840 [H]