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The map label for this gene is purF [H]

Identifier: 110680427

GI number: 110680427

Start: 3114831

End: 3116315

Strand: Direct

Name: purF [H]

Synonym: RD1_3247

Alternate gene names: 110680427

Gene position: 3114831-3116315 (Clockwise)

Preceding gene: 110680426

Following gene: 110680428

Centisome position: 75.36

GC content: 58.99

Gene sequence:

>1485_bases
ATGCCTCCCGCTCATCCGTTTGATACAAGTTATCTGCGCGACAGCAATGACGACGACAAACTGAAGGAAGAATGTGGGAT
CTTTGGGGTCATCGGTGTCGCCGATGCGTCCAATTTCGTGGCCCTTGGGCTGCATGCCCTGCAACATCGCGGACAAGAGG
CGGGCGGCATCGTGAGCTATGACGCGGTACAGGGCTTTCAATCCGCGCGCCGCTTTGGCTATGTGCGCGATAATTTCACG
AGTAAATCCGTGATGGAAACCCTGCCGGGTCCGCTGGCCATCGGGCATGTGCGCTATTCCACCGCCGGATCAAAAGGCCA
GACGGCCATCCGCGACGTACAGCCGTTCTTTGGCGAATTCGCCATGGGTGGGGCGGCGATTGCGCACAACGGCAACATTA
CAAACGCAAACGCCCTGCGCCGCGAGCTGATCGAACGCGGTTCGATTTTTCAAAGCTCGTCCGACAGTGAGTGCATCATT
CACCTGATGGCGCGATCCCTGCAACGCAACATCCCCGAACGTATGGAAGACGCCCTGCGCCGGGTGGAAGGCGCGTTTTC
GGTTGTCGCGATGACGCGCACCAAATTGATCGGCGTGCGCGATCCGCTTGGGGTACGCCCGCTTGTTCTGGGCAGGATCG
GGGATGGCTGGGCGTTGTCTTCGGAAACCTGTGCGCTGGATATCATCGGGGCCGAATTCATCCGCGAAATCGAACCCGGT
GAAATGGTCGTCATCACCGAAAAAGGGGTCGACAGCCAGTTCCCGTTCCGCCCGCAAAAACCACGCTTCTGCATCTTCGA
ACATGTGTATTTCTCGCGCCCCGACAGTATTCTGGGCGGGCGGTCGGTCTATGAAACGCGCGAAGCGATCGGGCGTGAAT
TGGCCAAGGAAAACCCGGTGGAGGCCGATCTTGTCTGCCCGGTACCGGATTCCGGCACCCCGGCGGCGATTGGCTATTCG
CTGGAATCGGGCATTCCCTATGCGATGGGGATCATCCGTAACCAATATATGGGCCGCACTTTTATCGAGCCGACTGAACA
GATCCGCAACATGGGCGTGCGCCTGAAACTGAACGTGAACCGCGCGCTGATCCAGGGCAAACGGGTTATTCTGGTGGACG
ACTCCGTGGTGCGCGGGACGACGAGCCGCAAGATCAAGGAAATGATCCTTGATGCCGGCGCCAAGGAAGTGCACTTCCGC
ATTGCCTCGCCGCCTACCGCCTGGCCCTGTTTTTATGGCGTCGATACGCCGCAACGCGAAAAACTGCTGGCCGCGACCAT
GTCCGAAGAGGAGATGCGCGCCCACCTACAGGTCGATAGCCTGAAATTCATCTCGCTGGATGGGCTTTATCGTGCCGTGG
GCGAGGCGAAGGGCCGCAACCCGAAACAGCCGCAATATTGCGATGCCTGTTTTTCCGGCGAATACCCGGTTGTGCCCTCG
GACCAGATCGAAGACGGCTTTGAAATGAAGCCCGCCGCCGAATAG

Upstream 100 bases:

>100_bases
CAGCACCAAGACCGTCATAGGTTCGTGACATAAGCCCGCGCAGGGCCTAAACAGTGCTCACCTGCAAACCGGATTCGGAG
CTGCCCCGTTGTCCAAGATC

Downstream 100 bases:

>100_bases
CCACCCTGTACAAACACTGCGGCGCGCTTTGGCCGTTACGCTGTTCTGACACTGACCTATCGTCGCAAGACGGCTTGACG
CCCGCGCGATTACACGGCAC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MPPAHPFDTSYLRDSNDDDKLKEECGIFGVIGVADASNFVALGLHALQHRGQEAGGIVSYDAVQGFQSARRFGYVRDNFT
SKSVMETLPGPLAIGHVRYSTAGSKGQTAIRDVQPFFGEFAMGGAAIAHNGNITNANALRRELIERGSIFQSSSDSECII
HLMARSLQRNIPERMEDALRRVEGAFSVVAMTRTKLIGVRDPLGVRPLVLGRIGDGWALSSETCALDIIGAEFIREIEPG
EMVVITEKGVDSQFPFRPQKPRFCIFEHVYFSRPDSILGGRSVYETREAIGRELAKENPVEADLVCPVPDSGTPAAIGYS
LESGIPYAMGIIRNQYMGRTFIEPTEQIRNMGVRLKLNVNRALIQGKRVILVDDSVVRGTTSRKIKEMILDAGAKEVHFR
IASPPTAWPCFYGVDTPQREKLLAATMSEEEMRAHLQVDSLKFISLDGLYRAVGEAKGRNPKQPQYCDACFSGEYPVVPS
DQIEDGFEMKPAAE

Sequences:

>Translated_494_residues
MPPAHPFDTSYLRDSNDDDKLKEECGIFGVIGVADASNFVALGLHALQHRGQEAGGIVSYDAVQGFQSARRFGYVRDNFT
SKSVMETLPGPLAIGHVRYSTAGSKGQTAIRDVQPFFGEFAMGGAAIAHNGNITNANALRRELIERGSIFQSSSDSECII
HLMARSLQRNIPERMEDALRRVEGAFSVVAMTRTKLIGVRDPLGVRPLVLGRIGDGWALSSETCALDIIGAEFIREIEPG
EMVVITEKGVDSQFPFRPQKPRFCIFEHVYFSRPDSILGGRSVYETREAIGRELAKENPVEADLVCPVPDSGTPAAIGYS
LESGIPYAMGIIRNQYMGRTFIEPTEQIRNMGVRLKLNVNRALIQGKRVILVDDSVVRGTTSRKIKEMILDAGAKEVHFR
IASPPTAWPCFYGVDTPQREKLLAATMSEEEMRAHLQVDSLKFISLDGLYRAVGEAKGRNPKQPQYCDACFSGEYPVVPS
DQIEDGFEMKPAAE
>Mature_493_residues
PPAHPFDTSYLRDSNDDDKLKEECGIFGVIGVADASNFVALGLHALQHRGQEAGGIVSYDAVQGFQSARRFGYVRDNFTS
KSVMETLPGPLAIGHVRYSTAGSKGQTAIRDVQPFFGEFAMGGAAIAHNGNITNANALRRELIERGSIFQSSSDSECIIH
LMARSLQRNIPERMEDALRRVEGAFSVVAMTRTKLIGVRDPLGVRPLVLGRIGDGWALSSETCALDIIGAEFIREIEPGE
MVVITEKGVDSQFPFRPQKPRFCIFEHVYFSRPDSILGGRSVYETREAIGRELAKENPVEADLVCPVPDSGTPAAIGYSL
ESGIPYAMGIIRNQYMGRTFIEPTEQIRNMGVRLKLNVNRALIQGKRVILVDDSVVRGTTSRKIKEMILDAGAKEVHFRI
ASPPTAWPCFYGVDTPQREKLLAATMSEEEMRAHLQVDSLKFISLDGLYRAVGEAKGRNPKQPQYCDACFSGEYPVVPSD
QIEDGFEMKPAAE

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=509, Percent_Identity=37.328094302554, Blast_Score=342, Evalue=4e-94,
Organism=Escherichia coli, GI1788651, Length=482, Percent_Identity=38.3817427385892, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI1790167, Length=233, Percent_Identity=29.6137339055794, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17554892, Length=477, Percent_Identity=38.9937106918239, Blast_Score=339, Evalue=3e-93,
Organism=Saccharomyces cerevisiae, GI6323958, Length=492, Percent_Identity=33.9430894308943, Blast_Score=243, Evalue=5e-65,
Organism=Drosophila melanogaster, GI24659598, Length=493, Percent_Identity=40.3651115618661, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24659604, Length=465, Percent_Identity=41.2903225806452, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI28573187, Length=506, Percent_Identity=39.9209486166008, Blast_Score=362, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 54380; Mature: 54248

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPAHPFDTSYLRDSNDDDKLKEECGIFGVIGVADASNFVALGLHALQHRGQEAGGIVSY
CCCCCCCCCHHCCCCCCHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEH
DAVQGFQSARRFGYVRDNFTSKSVMETLPGPLAIGHVRYSTAGSKGQTAIRDVQPFFGEF
HHHHHHHHHHHCCCHHCCCCHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHH
AMGGAAIAHNGNITNANALRRELIERGSIFQSSSDSECIIHLMARSLQRNIPERMEDALR
HCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
RVEGAFSVVAMTRTKLIGVRDPLGVRPLVLGRIGDGWALSSETCALDIIGAEFIREIEPG
HHHHHHHHHHHHHHHEEECCCCCCCCCEEEEECCCCEEECCCCEEEEEHHHHHHHHCCCC
EMVVITEKGVDSQFPFRPQKPRFCIFEHVYFSRPDSILGGRSVYETREAIGRELAKENPV
CEEEEEECCCCCCCCCCCCCCCEEEEEHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
EADLVCPVPDSGTPAAIGYSLESGIPYAMGIIRNQYMGRTFIEPTEQIRNMGVRLKLNVN
CCEEEECCCCCCCCCEEEEEHHCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCEEEEEECC
RALIQGKRVILVDDSVVRGTTSRKIKEMILDAGAKEVHFRIASPPTAWPCFYGVDTPQRE
HHHHCCCEEEEECCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCHHH
KLLAATMSEEEMRAHLQVDSLKFISLDGLYRAVGEAKGRNPKQPQYCDACFSGEYPVVPS
HHHHHHCCHHHHHHHHEECCEEEEEHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCC
DQIEDGFEMKPAAE
HHCCCCCCCCCCCC
>Mature Secondary Structure 
PPAHPFDTSYLRDSNDDDKLKEECGIFGVIGVADASNFVALGLHALQHRGQEAGGIVSY
CCCCCCCCHHCCCCCCHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEH
DAVQGFQSARRFGYVRDNFTSKSVMETLPGPLAIGHVRYSTAGSKGQTAIRDVQPFFGEF
HHHHHHHHHHHCCCHHCCCCHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHH
AMGGAAIAHNGNITNANALRRELIERGSIFQSSSDSECIIHLMARSLQRNIPERMEDALR
HCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
RVEGAFSVVAMTRTKLIGVRDPLGVRPLVLGRIGDGWALSSETCALDIIGAEFIREIEPG
HHHHHHHHHHHHHHHEEECCCCCCCCCEEEEECCCCEEECCCCEEEEEHHHHHHHHCCCC
EMVVITEKGVDSQFPFRPQKPRFCIFEHVYFSRPDSILGGRSVYETREAIGRELAKENPV
CEEEEEECCCCCCCCCCCCCCCEEEEEHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
EADLVCPVPDSGTPAAIGYSLESGIPYAMGIIRNQYMGRTFIEPTEQIRNMGVRLKLNVN
CCEEEECCCCCCCCCEEEEEHHCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCEEEEEECC
RALIQGKRVILVDDSVVRGTTSRKIKEMILDAGAKEVHFRIASPPTAWPCFYGVDTPQRE
HHHHCCCEEEEECCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCHHH
KLLAATMSEEEMRAHLQVDSLKFISLDGLYRAVGEAKGRNPKQPQYCDACFSGEYPVVPS
HHHHHHCCHHHHHHHHEECCEEEEEHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCC
DQIEDGFEMKPAAE
HHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA