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The map label for this gene is yafC [H]

Identifier: 110680412

GI number: 110680412

Start: 3098694

End: 3099599

Strand: Direct

Name: yafC [H]

Synonym: RD1_3231

Alternate gene names: 110680412

Gene position: 3098694-3099599 (Clockwise)

Preceding gene: 110680401

Following gene: 110680413

Centisome position: 74.97

GC content: 57.4

Gene sequence:

>906_bases
ATGGACTGGGACAAGTTGAGAATATTTCATGCCGTTGCGGATGCGGGATCGCTTACGCATGCGGGTGACAAGTTGAACCT
GTCCCAATCCGCTGTCAGCCGTCAGATTCGCGGGCTTGAGGAATCGCTGAACGCGACCCTGTTTCACCGCCATGCGCGCG
GGTTGATTCTGACCGAACAGGGCGAATTGTTGTTCGATGCAACCGCCGCCATGACCCGGCGTCTTGATACGGCCTTTGCC
CGCATTCGCGACAGCGAAGAAGAAGTTTTCGGCGAGTTGCGCGTGACGACAACAACCGGGTTTGGCACCTTGTGGCTGTC
GCCGCGCCTGCCAAAGCTTTATGAACGATACCCCGACCTCAAGGTGGATTTGATGCTCGAAGAGCGCGTTCTGGATCTGC
CGATGCGCGAGGCCGATGTGGCAATCCGCATGAAGGAGCCCAGCCAGGCCGATCTGATCCGCAAGAAACTGATGATGGTG
CGCATGTGCCTTTACGCCACGCCTGATTATCTGGCGGACCACGCCGCGCCGAGGACCCTCGAAGAGATCAAGGACCATCG
CCTGATCTGCCAGAACACCGATAGTGATCAGGTTGGCGCAGGCCGCAGCCTGGTGCAGCACCTGATGACACAAAACATCC
GCTCGATGCTGACGGTAAACAATTACTATGGCGTCTTGCAGGGCGTCTTGCAGAATCTGGGCATCGGGGTCTTGCCCGAT
TACGTCACTGAGGACTTCCCCGATCTGGTGCAGGTGCTGCCGGATCTCACCTCAGCGGATGTACCTGTGTTCCTGGCCTA
CCCCGAAGAACTGCGCCAGTCGCAACGCGTTTCGGCCTTCAAGGACTTCGTTCAGGATGAAATCATCAGCTATCGCAAAC
GGTTACGGGCAAATTCCGATAGCTGA

Upstream 100 bases:

>100_bases
GCCTAGGAAAATTTGTGCGAATTCACGCTTTCCCGCTCCGCGCCCCTGTCTTTCGTATCGAGATAGCGCTACCACACTGA
TGCGGACGGAGAGGGTGGAT

Downstream 100 bases:

>100_bases
CGGCGCGAGACATGCACGAAATGCATAGCGGACATGTCTTCATTTGTAGGCAAACTGCCTTGATTGAAGGCAGGTTGCCC
ATTAATTCACGTTTGTGACA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MDWDKLRIFHAVADAGSLTHAGDKLNLSQSAVSRQIRGLEESLNATLFHRHARGLILTEQGELLFDATAAMTRRLDTAFA
RIRDSEEEVFGELRVTTTTGFGTLWLSPRLPKLYERYPDLKVDLMLEERVLDLPMREADVAIRMKEPSQADLIRKKLMMV
RMCLYATPDYLADHAAPRTLEEIKDHRLICQNTDSDQVGAGRSLVQHLMTQNIRSMLTVNNYYGVLQGVLQNLGIGVLPD
YVTEDFPDLVQVLPDLTSADVPVFLAYPEELRQSQRVSAFKDFVQDEIISYRKRLRANSDS

Sequences:

>Translated_301_residues
MDWDKLRIFHAVADAGSLTHAGDKLNLSQSAVSRQIRGLEESLNATLFHRHARGLILTEQGELLFDATAAMTRRLDTAFA
RIRDSEEEVFGELRVTTTTGFGTLWLSPRLPKLYERYPDLKVDLMLEERVLDLPMREADVAIRMKEPSQADLIRKKLMMV
RMCLYATPDYLADHAAPRTLEEIKDHRLICQNTDSDQVGAGRSLVQHLMTQNIRSMLTVNNYYGVLQGVLQNLGIGVLPD
YVTEDFPDLVQVLPDLTSADVPVFLAYPEELRQSQRVSAFKDFVQDEIISYRKRLRANSDS
>Mature_301_residues
MDWDKLRIFHAVADAGSLTHAGDKLNLSQSAVSRQIRGLEESLNATLFHRHARGLILTEQGELLFDATAAMTRRLDTAFA
RIRDSEEEVFGELRVTTTTGFGTLWLSPRLPKLYERYPDLKVDLMLEERVLDLPMREADVAIRMKEPSQADLIRKKLMMV
RMCLYATPDYLADHAAPRTLEEIKDHRLICQNTDSDQVGAGRSLVQHLMTQNIRSMLTVNNYYGVLQGVLQNLGIGVLPD
YVTEDFPDLVQVLPDLTSADVPVFLAYPEELRQSQRVSAFKDFVQDEIISYRKRLRANSDS

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=290, Percent_Identity=27.5862068965517, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787128, Length=310, Percent_Identity=26.7741935483871, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789440, Length=290, Percent_Identity=27.5862068965517, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87081978, Length=287, Percent_Identity=26.8292682926829, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI145693193, Length=291, Percent_Identity=24.3986254295533, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787589, Length=284, Percent_Identity=25, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789173, Length=297, Percent_Identity=24.5791245791246, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI157672245, Length=137, Percent_Identity=37.2262773722628, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789639, Length=295, Percent_Identity=22.0338983050847, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787601, Length=187, Percent_Identity=26.2032085561497, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788706, Length=178, Percent_Identity=29.7752808988764, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790262, Length=251, Percent_Identity=24.3027888446215, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786448, Length=142, Percent_Identity=31.6901408450704, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788297, Length=176, Percent_Identity=24.4318181818182, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34239; Mature: 34239

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDWDKLRIFHAVADAGSLTHAGDKLNLSQSAVSRQIRGLEESLNATLFHRHARGLILTEQ
CCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECC
GELLFDATAAMTRRLDTAFARIRDSEEEVFGELRVTTTTGFGTLWLSPRLPKLYERYPDL
CCEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEEECCCEEEEECCCCCHHHHHCCCC
KVDLMLEERVLDLPMREADVAIRMKEPSQADLIRKKLMMVRMCLYATPDYLADHAAPRTL
EEEHEEHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHH
EEIKDHRLICQNTDSDQVGAGRSLVQHLMTQNIRSMLTVNNYYGVLQGVLQNLGIGVLPD
HHHHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
YVTEDFPDLVQVLPDLTSADVPVFLAYPEELRQSQRVSAFKDFVQDEIISYRKRLRANSD
HHHCCHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
S
C
>Mature Secondary Structure
MDWDKLRIFHAVADAGSLTHAGDKLNLSQSAVSRQIRGLEESLNATLFHRHARGLILTEQ
CCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECC
GELLFDATAAMTRRLDTAFARIRDSEEEVFGELRVTTTTGFGTLWLSPRLPKLYERYPDL
CCEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEEECCCEEEEECCCCCHHHHHCCCC
KVDLMLEERVLDLPMREADVAIRMKEPSQADLIRKKLMMVRMCLYATPDYLADHAAPRTL
EEEHEEHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHH
EEIKDHRLICQNTDSDQVGAGRSLVQHLMTQNIRSMLTVNNYYGVLQGVLQNLGIGVLPD
HHHHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
YVTEDFPDLVQVLPDLTSADVPVFLAYPEELRQSQRVSAFKDFVQDEIISYRKRLRANSD
HHHCCHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]