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The map label for this gene is ost [H]

Identifier: 110680308

GI number: 110680308

Start: 2997722

End: 2999845

Strand: Direct

Name: ost [H]

Synonym: RD1_3121

Alternate gene names: 110680308

Gene position: 2997722-2999845 (Clockwise)

Preceding gene: 110680307

Following gene: 110680309

Centisome position: 72.53

GC content: 59.51

Gene sequence:

>2124_bases
ATGATGTTGCGCGGCTTACTCATTCTCGTTCTGGCGCTTGTCCCCTGCCTTGCGACCGCGCAGCAAGGCGAACCACCGCG
CCCAGCCACATTGATCGCCGATGAGATTTTCATTGCCGCCGACCGCGAATTGGTCGCGCGTGGCAACGTCGAAGCGCTTC
AGGGCCAAACCCGCCTGCGGGCAGAAGAAATCCGCTATGATCGTGTCACCGGTGCGCTGACCATCGTTGGGCCCATTCGG
ATACAGGATGGCGACAGCATCACGGTTCTGGCCAGCGCCGCCGAATTGGATGCAACGCTTCAGACCGGGCTTCTGAGCGG
GGCGCGCATGGTGATCAACCAGCAACTTCAACTGGCAAGCGCGCAAATGGAGCGCGTGAGCGAGCGTTACAGCCAGCTCT
ATCGCAGTGCGGTCACCTCCTGCGACGTCTGCGACGATGGCAAACCGCCGCTGTGGCAGATCAGGGCGCGGCGCGTCATC
CATGACCGTCAGGAACGCCAGATCTATTTTGAGGACGCACAGTTTCGCATCCGCAACGTGCCCGTGTTCTATTTTCCGCG
CCTGCGCCTGCCGGACCCGACAGTGGAACGCGCCACCGGTTTCCTGATCCCGTCGCTGGTCAGCACATCCGAATTCGGGC
TGGGCATCAAAGTGCCCTATTTCATTGCGATTGGCGATGACCGCGACCTAACGGTCACGCCCTATTATTCAAGCAGCACG
CGAACTGTTGAGATGCGCTACAGGCAGGCCTTTGTAAACGGTGACATCGAATTCGACTGGGCCATCAGCCGCGATGATGA
ACGCCCCGGAGAAACGCGCGGATATCTGTTCGGCAACGGGTCATTCGATCTGCAGAATGATTTCAAGCTGAGGTTCGATG
TCGAACTGACATCCGATGACGATTATCTGCAGGACTATTCCTTTTCCGGCAAGGATCGGCTGGACAGTCAGATCACCATC
AGCCGCGCGCGAAAAGACGAATACACCGCAGGAAGCCTGATTGGGTACAAATCACTGCGTGACAACGAACAAAATTCGAC
CATCCCCACCATTGTCGGCGATATCTTCTACCAGCGCCGTTTTTTCCCGGCCGCCAGCGGCGGGGAGCTGCGCTTTACCG
TCAATGGGCACAATCACATCCGATATTCGGACAGAGACATCGTCGGGCGGGACGTGTCCCGCGTGAACACCGAGGCCGAA
TGGCTGCGGGGCTGGACCCTGCCCGGCGGCCTGCGCGCCGAAACCCGGTTGGGCGTTGCCGCAGATATCTTCAACATCAC
GCAAGACAGCAGCACCAATCAGAACCAGTCGCAGATTTCACCCTTCAGCACCGTGGCGCTGCGGTATCCGATGACCCGCG
TGGAGGACAGCGGGGCGACGCAGTTCCTCGAACCGATTGCGCAACTTGCATGGACAGGCTCCACACAGCTGGACATCCCG
AACGAAGAAAGCACGCTGGTGGATTTTGACGACGGCAACCTGCTGGCGCTGTCCCGTTTCCCCAGCCCGGACCGGCGCGA
GCGTGGCGGCGTGGGAGCCTTTGGCGTGAACTGGTCCCGCTTTGCACTGTCCGGATGGGAAAGCGCGCTGACGATGGGTC
AGGTGATACGCAACGACGAAGACGAGAATTTCACCGAGGCCTCGGGCCTGTCCGGGCTGCGGTCGGATTATCTGGTGGCT
GGGCAGATCAAAACGCTCAACGGCTGGGCCGTGACGGCGCGCACGCTGTTCGATGACAGTTTCAGCTTCAGCAAGGCCGA
GGTGCGCGGGTCCTTTGATCACAGGCTTGGCGATCTCAGCGGCAGTTATCTGTGGCTCACGCGTGACGCGCAGGAAAGCC
GCTTCATCAATACCTCCGAACTGTTTCTGGACGGCTCCTACATCGTGGATCGCAACTGGCGCGCGACAGCGGACTGGCGC
TATGACCTGATTGCGGATCGGGCGTCGAACGCGGGTCTTAGCCTGACGTACTTTAACGAATGCGTGGAAGTCGATTTTTC
CGTGCGCCGCCGATATACCTCTTCAACAAGTATTGAGCCTTCCACCAGTTTCGGCTTATCCATCGGGCTGTCGGGCTTTT
CGGCCAAACGGGGAACGGACACTTATGCCAAAACATGCGGGTGA

Upstream 100 bases:

>100_bases
TCGGGGAAAACGGCCAGTTGCCTGTTTACCTCGCCGCATGGGCACCACCAATCGCGTCGGTCTTGCTGGCTTTCGGCCCG
CTGCTACATGCAGAGGACGG

Downstream 100 bases:

>100_bases
CAGACAGATGATCCGAATTAAACAGTTTTGCAGCGTCCTTGCGCTTTGTGCTGCATGCAGCCTTGCCCCGCCCCTTGGCG
CGCAAAACCTGTTCAGCCCG

Product: organic solvent tolerance protein, putative

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 707; Mature: 707

Protein sequence:

>707_residues
MMLRGLLILVLALVPCLATAQQGEPPRPATLIADEIFIAADRELVARGNVEALQGQTRLRAEEIRYDRVTGALTIVGPIR
IQDGDSITVLASAAELDATLQTGLLSGARMVINQQLQLASAQMERVSERYSQLYRSAVTSCDVCDDGKPPLWQIRARRVI
HDRQERQIYFEDAQFRIRNVPVFYFPRLRLPDPTVERATGFLIPSLVSTSEFGLGIKVPYFIAIGDDRDLTVTPYYSSST
RTVEMRYRQAFVNGDIEFDWAISRDDERPGETRGYLFGNGSFDLQNDFKLRFDVELTSDDDYLQDYSFSGKDRLDSQITI
SRARKDEYTAGSLIGYKSLRDNEQNSTIPTIVGDIFYQRRFFPAASGGELRFTVNGHNHIRYSDRDIVGRDVSRVNTEAE
WLRGWTLPGGLRAETRLGVAADIFNITQDSSTNQNQSQISPFSTVALRYPMTRVEDSGATQFLEPIAQLAWTGSTQLDIP
NEESTLVDFDDGNLLALSRFPSPDRRERGGVGAFGVNWSRFALSGWESALTMGQVIRNDEDENFTEASGLSGLRSDYLVA
GQIKTLNGWAVTARTLFDDSFSFSKAEVRGSFDHRLGDLSGSYLWLTRDAQESRFINTSELFLDGSYIVDRNWRATADWR
YDLIADRASNAGLSLTYFNECVEVDFSVRRRYTSSTSIEPSTSFGLSIGLSGFSAKRGTDTYAKTCG

Sequences:

>Translated_707_residues
MMLRGLLILVLALVPCLATAQQGEPPRPATLIADEIFIAADRELVARGNVEALQGQTRLRAEEIRYDRVTGALTIVGPIR
IQDGDSITVLASAAELDATLQTGLLSGARMVINQQLQLASAQMERVSERYSQLYRSAVTSCDVCDDGKPPLWQIRARRVI
HDRQERQIYFEDAQFRIRNVPVFYFPRLRLPDPTVERATGFLIPSLVSTSEFGLGIKVPYFIAIGDDRDLTVTPYYSSST
RTVEMRYRQAFVNGDIEFDWAISRDDERPGETRGYLFGNGSFDLQNDFKLRFDVELTSDDDYLQDYSFSGKDRLDSQITI
SRARKDEYTAGSLIGYKSLRDNEQNSTIPTIVGDIFYQRRFFPAASGGELRFTVNGHNHIRYSDRDIVGRDVSRVNTEAE
WLRGWTLPGGLRAETRLGVAADIFNITQDSSTNQNQSQISPFSTVALRYPMTRVEDSGATQFLEPIAQLAWTGSTQLDIP
NEESTLVDFDDGNLLALSRFPSPDRRERGGVGAFGVNWSRFALSGWESALTMGQVIRNDEDENFTEASGLSGLRSDYLVA
GQIKTLNGWAVTARTLFDDSFSFSKAEVRGSFDHRLGDLSGSYLWLTRDAQESRFINTSELFLDGSYIVDRNWRATADWR
YDLIADRASNAGLSLTYFNECVEVDFSVRRRYTSSTSIEPSTSFGLSIGLSGFSAKRGTDTYAKTCG
>Mature_707_residues
MMLRGLLILVLALVPCLATAQQGEPPRPATLIADEIFIAADRELVARGNVEALQGQTRLRAEEIRYDRVTGALTIVGPIR
IQDGDSITVLASAAELDATLQTGLLSGARMVINQQLQLASAQMERVSERYSQLYRSAVTSCDVCDDGKPPLWQIRARRVI
HDRQERQIYFEDAQFRIRNVPVFYFPRLRLPDPTVERATGFLIPSLVSTSEFGLGIKVPYFIAIGDDRDLTVTPYYSSST
RTVEMRYRQAFVNGDIEFDWAISRDDERPGETRGYLFGNGSFDLQNDFKLRFDVELTSDDDYLQDYSFSGKDRLDSQITI
SRARKDEYTAGSLIGYKSLRDNEQNSTIPTIVGDIFYQRRFFPAASGGELRFTVNGHNHIRYSDRDIVGRDVSRVNTEAE
WLRGWTLPGGLRAETRLGVAADIFNITQDSSTNQNQSQISPFSTVALRYPMTRVEDSGATQFLEPIAQLAWTGSTQLDIP
NEESTLVDFDDGNLLALSRFPSPDRRERGGVGAFGVNWSRFALSGWESALTMGQVIRNDEDENFTEASGLSGLRSDYLVA
GQIKTLNGWAVTARTLFDDSFSFSKAEVRGSFDHRLGDLSGSYLWLTRDAQESRFINTSELFLDGSYIVDRNWRATADWR
YDLIADRASNAGLSLTYFNECVEVDFSVRRRYTSSTSIEPSTSFGLSIGLSGFSAKRGTDTYAKTCG

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=741, Percent_Identity=23.2118758434548, Blast_Score=96, Evalue=1e-20,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 79211; Mature: 79211

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLRGLLILVLALVPCLATAQQGEPPRPATLIADEIFIAADRELVARGNVEALQGQTRLR
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCHHEECCCHHHHCCCHHHH
AEEIRYDRVTGALTIVGPIRIQDGDSITVLASAAELDATLQTGLLSGARMVINQQLQLAS
HHHHHHHHCCEEEEEEEEEEECCCCEEEEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHH
AQMERVSERYSQLYRSAVTSCDVCDDGKPPLWQIRARRVIHDRQERQIYFEDAQFRIRNV
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCEEEEECC
PVFYFPRLRLPDPTVERATGFLIPSLVSTSEFGLGIKVPYFIAIGDDRDLTVTPYYSSST
CEEEECCCCCCCCHHHHHCCCHHHHHHCCCCCCCEEECCEEEEECCCCCEEEEEEECCCC
RTVEMRYRQAFVNGDIEFDWAISRDDERPGETRGYLFGNGSFDLQNDFKLRFDVELTSDD
EEEEEEEHHHHHCCCEEEEEEECCCCCCCCCCCCEEEECCCCCCCCCEEEEEEEEECCCC
DYLQDYSFSGKDRLDSQITISRARKDEYTAGSLIGYKSLRDNEQNSTIPTIVGDIFYQRR
CHHHCCCCCCCCCCCCEEEEEHHCCCCCCCCCEEEHHHHCCCCCCCCCHHHHHHHHHHHH
FFPAASGGELRFTVNGHNHIRYSDRDIVGRDVSRVNTEAEWLRGWTLPGGLRAETRLGVA
CCCCCCCCEEEEEECCCCEEEECCCCCCCCCHHHCCCCHHHHCCCCCCCCCCCCCCCCEE
ADIFNITQDSSTNQNQSQISPFSTVALRYPMTRVEDSGATQFLEPIAQLAWTGSTQLDIP
EHEEECCCCCCCCCCHHHCCCHHEEEEECCHHHCCCCCHHHHHHHHHHHHCCCCCEEECC
NEESTLVDFDDGNLLALSRFPSPDRRERGGVGAFGVNWSRFALSGWESALTMGQVIRNDE
CCCCEEEEECCCCEEEEECCCCCCCHHCCCCEEECCCCHHHHHCCHHHHHHHHHHHCCCC
DENFTEASGLSGLRSDYLVAGQIKTLNGWAVTARTLFDDSFSFSKAEVRGSFDHRLGDLS
CCCCHHHCCCCCCCCCCEEEEEEEEECCEEEEEEEEECCCCCCCHHHHCCCHHHHHCCCC
GSYLWLTRDAQESRFINTSELFLDGSYIVDRNWRATADWRYDLIADRASNAGLSLTYFNE
CCEEEEEECCCHHHCCCCHHEEEECCEEEECCCEECCCEEEEEEEECCCCCCEEEEEEHH
CVEVDFSVRRRYTSSTSIEPSTSFGLSIGLSGFSAKRGTDTYAKTCG
HHHCCHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHCCC
>Mature Secondary Structure
MMLRGLLILVLALVPCLATAQQGEPPRPATLIADEIFIAADRELVARGNVEALQGQTRLR
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCHHEECCCHHHHCCCHHHH
AEEIRYDRVTGALTIVGPIRIQDGDSITVLASAAELDATLQTGLLSGARMVINQQLQLAS
HHHHHHHHCCEEEEEEEEEEECCCCEEEEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHH
AQMERVSERYSQLYRSAVTSCDVCDDGKPPLWQIRARRVIHDRQERQIYFEDAQFRIRNV
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCEEEEECC
PVFYFPRLRLPDPTVERATGFLIPSLVSTSEFGLGIKVPYFIAIGDDRDLTVTPYYSSST
CEEEECCCCCCCCHHHHHCCCHHHHHHCCCCCCCEEECCEEEEECCCCCEEEEEEECCCC
RTVEMRYRQAFVNGDIEFDWAISRDDERPGETRGYLFGNGSFDLQNDFKLRFDVELTSDD
EEEEEEEHHHHHCCCEEEEEEECCCCCCCCCCCCEEEECCCCCCCCCEEEEEEEEECCCC
DYLQDYSFSGKDRLDSQITISRARKDEYTAGSLIGYKSLRDNEQNSTIPTIVGDIFYQRR
CHHHCCCCCCCCCCCCEEEEEHHCCCCCCCCCEEEHHHHCCCCCCCCCHHHHHHHHHHHH
FFPAASGGELRFTVNGHNHIRYSDRDIVGRDVSRVNTEAEWLRGWTLPGGLRAETRLGVA
CCCCCCCCEEEEEECCCCEEEECCCCCCCCCHHHCCCCHHHHCCCCCCCCCCCCCCCCEE
ADIFNITQDSSTNQNQSQISPFSTVALRYPMTRVEDSGATQFLEPIAQLAWTGSTQLDIP
EHEEECCCCCCCCCCHHHCCCHHEEEEECCHHHCCCCCHHHHHHHHHHHHCCCCCEEECC
NEESTLVDFDDGNLLALSRFPSPDRRERGGVGAFGVNWSRFALSGWESALTMGQVIRNDE
CCCCEEEEECCCCEEEEECCCCCCCHHCCCCEEECCCCHHHHHCCHHHHHHHHHHHCCCC
DENFTEASGLSGLRSDYLVAGQIKTLNGWAVTARTLFDDSFSFSKAEVRGSFDHRLGDLS
CCCCHHHCCCCCCCCCCEEEEEEEEECCEEEEEEEEECCCCCCCHHHHCCCHHHHHCCCC
GSYLWLTRDAQESRFINTSELFLDGSYIVDRNWRATADWRYDLIADRASNAGLSLTYFNE
CCEEEEEECCCHHHCCCCHHEEEECCEEEECCCEECCCEEEEEEEECCCCCCEEEEEEHH
CVEVDFSVRRRYTSSTSIEPSTSFGLSIGLSGFSAKRGTDTYAKTCG
HHHCCHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA