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The map label for this gene is rrmJ

Identifier: 110680244

GI number: 110680244

Start: 2922661

End: 2923383

Strand: Reverse

Name: rrmJ

Synonym: RD1_3052

Alternate gene names: 110680244

Gene position: 2923383-2922661 (Counterclockwise)

Preceding gene: 110680245

Following gene: 110680243

Centisome position: 70.73

GC content: 60.17

Gene sequence:

>723_bases
ATGGCGAAAAAACCGGGCAGTCAAAATACATCGGGGCGCGGGCAGCGTGATCTGAAGGTCAAGGTCAAGACCGCGCGCGG
GCGCAAGCTGAGTTCAACGCGCTGGCTTCAGCGTCAGTTGAACGACCCATATGTAAAACGCGCCCGCAGTGAGGGGTATC
GCGGGCGGGCGGCCTATAAGATACTGGAGTTGGATGACAGGTTCCGGTTTCTGGTGCCGGGTGCGCGTGTGGTGGATCTG
GGCTGTGCGCCCGGTGGCTGGTGTCAGGTGGCGGTCAAGCGCGTGAATGCGCTGGGAGAGCGGACAAGCAAGGCGCAGGG
CAGCGTTCTGGGGGTTGATCTGCAGGAGATGGAGCCGATCGCGGGCGCGACGCTCTATCAGCTGGATTTCATGGCCGATG
ACGCGGATGAACAGGTGCGCGTCTGGTTGGGTGGCAAGGCCGATGTGGTGATGTCCGATATGGCCGCGTCAAGCTCGGGG
CACAAGCAGACGGACCATTTGCGCATCATAGCGCTATGCGAAGCGGCGGCATATTTTGCATTTGATGTTCTGGAGGAGGG
CGGCACCTTTGTCGCGAAAGTGCTCGCAGGTGGGGCGGAAGGTGAGTTGCAAAAGCTGCTGAAGCAACGGTTTGCAAAGG
TTGCCAACATCAAACCCCCGGCCTCCCGGCAGGACAGTTCGGAGAAATTTGTCGTCGCAACTGGCTTTCGCGCTAAAGCC
TGA

Upstream 100 bases:

>100_bases
GGCCAACGGATCGGTTGTCGGTGGCGGATCGTGGCCTGCGCGAAGGGCTGCTTTATGCGCAGATGAGTGCGCAGGGTGTG
TTGGAAGACGGGGCGTTCTG

Downstream 100 bases:

>100_bases
GCAATTTTTACCTCGTATTAAAGTCAAGCTGCTTAGCATGTGCCAGAAGCCGTGCCATTTCGGCCGGTCGTATCTTAATG
AGGGAACCCGAACATGTTTC

Product: ribosomal RNA large subunit methyltransferase, putative

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MAKKPGSQNTSGRGQRDLKVKVKTARGRKLSSTRWLQRQLNDPYVKRARSEGYRGRAAYKILELDDRFRFLVPGARVVDL
GCAPGGWCQVAVKRVNALGERTSKAQGSVLGVDLQEMEPIAGATLYQLDFMADDADEQVRVWLGGKADVVMSDMAASSSG
HKQTDHLRIIALCEAAAYFAFDVLEEGGTFVAKVLAGGAEGELQKLLKQRFAKVANIKPPASRQDSSEKFVVATGFRAKA

Sequences:

>Translated_240_residues
MAKKPGSQNTSGRGQRDLKVKVKTARGRKLSSTRWLQRQLNDPYVKRARSEGYRGRAAYKILELDDRFRFLVPGARVVDL
GCAPGGWCQVAVKRVNALGERTSKAQGSVLGVDLQEMEPIAGATLYQLDFMADDADEQVRVWLGGKADVVMSDMAASSSG
HKQTDHLRIIALCEAAAYFAFDVLEEGGTFVAKVLAGGAEGELQKLLKQRFAKVANIKPPASRQDSSEKFVVATGFRAKA
>Mature_239_residues
AKKPGSQNTSGRGQRDLKVKVKTARGRKLSSTRWLQRQLNDPYVKRARSEGYRGRAAYKILELDDRFRFLVPGARVVDLG
CAPGGWCQVAVKRVNALGERTSKAQGSVLGVDLQEMEPIAGATLYQLDFMADDADEQVRVWLGGKADVVMSDMAASSSGH
KQTDHLRIIALCEAAAYFAFDVLEEGGTFVAKVLAGGAEGELQKLLKQRFAKVANIKPPASRQDSSEKFVVATGFRAKA

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=207, Percent_Identity=44.4444444444444, Blast_Score=178, Evalue=4e-45,
Organism=Homo sapiens, GI29029591, Length=196, Percent_Identity=35.7142857142857, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI29029589, Length=196, Percent_Identity=35.7142857142857, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI7110661, Length=196, Percent_Identity=35.7142857142857, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI194097365, Length=200, Percent_Identity=34.5, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1789569, Length=213, Percent_Identity=38.4976525821596, Blast_Score=135, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17553474, Length=211, Percent_Identity=36.4928909952607, Blast_Score=147, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17554650, Length=200, Percent_Identity=35.5, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17553860, Length=206, Percent_Identity=33.495145631068, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71987550, Length=206, Percent_Identity=33.495145631068, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71987561, Length=206, Percent_Identity=33.495145631068, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6319535, Length=202, Percent_Identity=35.6435643564356, Blast_Score=112, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6319796, Length=201, Percent_Identity=34.8258706467662, Blast_Score=102, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6321302, Length=137, Percent_Identity=33.5766423357664, Blast_Score=90, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21356387, Length=221, Percent_Identity=39.3665158371041, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24648639, Length=196, Percent_Identity=36.2244897959184, Blast_Score=114, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=37.3737373737374, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI18859957, Length=202, Percent_Identity=35.6435643564356, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_ROSDO (Q164M8)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_683251.1
- ProteinModelPortal:   Q164M8
- SMR:   Q164M8
- STRING:   Q164M8
- GeneID:   4198755
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_3052
- NMPDR:   fig|375451.6.peg.2838
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- PhylomeDB:   Q164M8
- ProtClustDB:   CLSK933653
- BioCyc:   RDEN375451:RD1_3052-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 26161; Mature: 26030

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: NA

Important sites: ACT_SITE 193-193 BINDING 85-85 BINDING 87-87 BINDING 113-113 BINDING 129-129 BINDING 153-153

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKPGSQNTSGRGQRDLKVKVKTARGRKLSSTRWLQRQLNDPYVKRARSEGYRGRAAYK
CCCCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCEEE
ILELDDRFRFLVPGARVVDLGCAPGGWCQVAVKRVNALGERTSKAQGSVLGVDLQEMEPI
EEEECCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHCCCC
AGATLYQLDFMADDADEQVRVWLGGKADVVMSDMAASSSGHKQTDHLRIIALCEAAAYFA
CCCEEEEEEEECCCCCCEEEEEECCCCCEEHHHHHCCCCCCCCCCCEEEEHHHHHHHHHH
FDVLEEGGTFVAKVLAGGAEGELQKLLKQRFAKVANIKPPASRQDSSEKFVVATGFRAKA
HHHHHCCCCEEEHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCC
>Mature Secondary Structure 
AKKPGSQNTSGRGQRDLKVKVKTARGRKLSSTRWLQRQLNDPYVKRARSEGYRGRAAYK
CCCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCEEE
ILELDDRFRFLVPGARVVDLGCAPGGWCQVAVKRVNALGERTSKAQGSVLGVDLQEMEPI
EEEECCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHCCCC
AGATLYQLDFMADDADEQVRVWLGGKADVVMSDMAASSSGHKQTDHLRIIALCEAAAYFA
CCCEEEEEEEECCCCCCEEEEEECCCCCEEHHHHHCCCCCCCCCCCEEEEHHHHHHHHHH
FDVLEEGGTFVAKVLAGGAEGELQKLLKQRFAKVANIKPPASRQDSSEKFVVATGFRAKA
HHHHHCCCCEEEHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA