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The map label for this gene is lpdA [H]

Identifier: 110680193

GI number: 110680193

Start: 2871981

End: 2873375

Strand: Reverse

Name: lpdA [H]

Synonym: RD1_2995

Alternate gene names: 110680193

Gene position: 2873375-2871981 (Counterclockwise)

Preceding gene: 110680194

Following gene: 110680191

Centisome position: 69.52

GC content: 58.21

Gene sequence:

>1395_bases
ATGGCATCCAAATCCTTTGATCTTATCGTGATCGGAGCCGGTCCGGGCGGCTATGTCGCGGCGATACGCGGCGCGCAACT
GGGCATGTCAGTGGCTATCGTTGAACGCGAGCATCTGGGCGGCATCTGTCTGAACTGGGGATGCATCCCGACCAAGGCGA
TGCTGCGGTCCTCCGAAGTGTTCCACCTGATGCAACGGGCCAAGGAATTCGGGCTCAAGGCGGAAAAGGTGGATTATGAC
CTCGACGCGGTTGTCAAACGCTCCCGCAAGGTGGCGGGGCAATTGTCCGGCGGCATTGGCCATCTGATGAAGAAAAACAA
GGTCACCGTTTTCATGGGTGAGGCCAAACTTGCCGGTAAAGGCAAGGTGAGCGTCAAGACCGACAAGGGGGCGGAAGACC
TCACTGCCAAGAACATCGTGCTCGCCACCGGCGCGCGCGCCCGCACCCTGCCGGGGCTTGAGGCGGATGGCGATCTGGTC
TGGACCTACAAACACGCGCTTGAACCGAAGCACATGCCCAAAAAGCTGCTGGTCATCGGATCGGGAGCCATCGGTATCGA
ATTCGCCAGTTTTTACAACACTCTGGGCGCGGATACGACAGTCGTCGAAGTCATGGACCGCGTGCTGCCGGTCGAGGATG
AAGAGATTTCCGCCTTTGCCAAGAAAGCCTTTGAAAAGCAGGGCATGAAGATCATGCAGAAAGCCATGGTCAAGCAATTG
GACCGGGCAAAAGGCAAGGTCACCGCGCATATTGAAGTGGGCGGTAAGGTTGAAAAACATGAATTCGACACTGTCATCTC
GGCGGTCGGGATCGTCGGAAATGTCGAAGGGCTGGGCCTTGAGGAATTGGGCGTCAAGATCGACCGCAGCCATGTGGTCA
CGGATGAATTCTGCCGCACCGGGATCGAGGGGCTCTATGCCATCGGTGATGTGGCCGGCGCGCCGTGGCTTGCGCATAAG
GCCAGCCATGAAGGGGTCATGGTCGCGGATCTGATCGCGGGCAAACACGCCCATCCCGTCAAGCCGGAAACCATTGCCGG
CTGCACCTATTGCCATCCGCAGGTCGCCTCCGTGGGCTACAGTGAGGCAAAGGCCAAGGAGCTGGGCTATACAGTCAAGG
TCGGACGTTTCCCGTTTATCGGCAACGGCAAAGCCATCGCACTTGGCGAGCCGGAAGGCATGATCAAGACCGTCTTCGAT
GCCAAGACAGGCGAACTGCTCGGCGCGCATATGGTGGGTGCCGAAGTCACCGAACTGATCCAAGGCTATGTGGTCGGGCG
TCAGCTGGAAACAACCGAAGAAGACCTGATGAACACCGTCTTCCCGCATCCGACGCTGTCAGAAATGATGCATGAAAGCG
TGCTGGACGCGTACGATCGCGTGATACACATGTAG

Upstream 100 bases:

>100_bases
TCCGACAGTGCGCATTTGTCGCGCGACTGACCGTGTTGCTGTGAAACTCGATATAGTTTAACGGTAAACTATATTTGACT
CACAGGCGCGAGGGCACAGC

Downstream 100 bases:

>100_bases
GATCGCATCGACACAAAAAAGCGGGGATCAAACCCCGCTTTTGTCGTGTTTATGACGCTGTTGAAAGGTTGAAGTCCTAA
TTAGAGCGTCTGACGAAATA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MASKSFDLIVIGAGPGGYVAAIRGAQLGMSVAIVEREHLGGICLNWGCIPTKAMLRSSEVFHLMQRAKEFGLKAEKVDYD
LDAVVKRSRKVAGQLSGGIGHLMKKNKVTVFMGEAKLAGKGKVSVKTDKGAEDLTAKNIVLATGARARTLPGLEADGDLV
WTYKHALEPKHMPKKLLVIGSGAIGIEFASFYNTLGADTTVVEVMDRVLPVEDEEISAFAKKAFEKQGMKIMQKAMVKQL
DRAKGKVTAHIEVGGKVEKHEFDTVISAVGIVGNVEGLGLEELGVKIDRSHVVTDEFCRTGIEGLYAIGDVAGAPWLAHK
ASHEGVMVADLIAGKHAHPVKPETIAGCTYCHPQVASVGYSEAKAKELGYTVKVGRFPFIGNGKAIALGEPEGMIKTVFD
AKTGELLGAHMVGAEVTELIQGYVVGRQLETTEEDLMNTVFPHPTLSEMMHESVLDAYDRVIHM

Sequences:

>Translated_464_residues
MASKSFDLIVIGAGPGGYVAAIRGAQLGMSVAIVEREHLGGICLNWGCIPTKAMLRSSEVFHLMQRAKEFGLKAEKVDYD
LDAVVKRSRKVAGQLSGGIGHLMKKNKVTVFMGEAKLAGKGKVSVKTDKGAEDLTAKNIVLATGARARTLPGLEADGDLV
WTYKHALEPKHMPKKLLVIGSGAIGIEFASFYNTLGADTTVVEVMDRVLPVEDEEISAFAKKAFEKQGMKIMQKAMVKQL
DRAKGKVTAHIEVGGKVEKHEFDTVISAVGIVGNVEGLGLEELGVKIDRSHVVTDEFCRTGIEGLYAIGDVAGAPWLAHK
ASHEGVMVADLIAGKHAHPVKPETIAGCTYCHPQVASVGYSEAKAKELGYTVKVGRFPFIGNGKAIALGEPEGMIKTVFD
AKTGELLGAHMVGAEVTELIQGYVVGRQLETTEEDLMNTVFPHPTLSEMMHESVLDAYDRVIHM
>Mature_463_residues
ASKSFDLIVIGAGPGGYVAAIRGAQLGMSVAIVEREHLGGICLNWGCIPTKAMLRSSEVFHLMQRAKEFGLKAEKVDYDL
DAVVKRSRKVAGQLSGGIGHLMKKNKVTVFMGEAKLAGKGKVSVKTDKGAEDLTAKNIVLATGARARTLPGLEADGDLVW
TYKHALEPKHMPKKLLVIGSGAIGIEFASFYNTLGADTTVVEVMDRVLPVEDEEISAFAKKAFEKQGMKIMQKAMVKQLD
RAKGKVTAHIEVGGKVEKHEFDTVISAVGIVGNVEGLGLEELGVKIDRSHVVTDEFCRTGIEGLYAIGDVAGAPWLAHKA
SHEGVMVADLIAGKHAHPVKPETIAGCTYCHPQVASVGYSEAKAKELGYTVKVGRFPFIGNGKAIALGEPEGMIKTVFDA
KTGELLGAHMVGAEVTELIQGYVVGRQLETTEEDLMNTVFPHPTLSEMMHESVLDAYDRVIHM

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=461, Percent_Identity=38.6117136659436, Blast_Score=297, Evalue=1e-80,
Organism=Homo sapiens, GI50301238, Length=459, Percent_Identity=27.6688453159041, Blast_Score=165, Evalue=9e-41,
Organism=Homo sapiens, GI22035672, Length=464, Percent_Identity=27.801724137931, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI33519430, Length=464, Percent_Identity=25.2155172413793, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI33519428, Length=464, Percent_Identity=25.2155172413793, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI33519426, Length=464, Percent_Identity=25.2155172413793, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI148277065, Length=464, Percent_Identity=25.2155172413793, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI148277071, Length=464, Percent_Identity=25.2155172413793, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI291045266, Length=427, Percent_Identity=25.2927400468384, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI291045268, Length=423, Percent_Identity=26.0047281323877, Blast_Score=107, Evalue=4e-23,
Organism=Escherichia coli, GI1786307, Length=452, Percent_Identity=35.8407079646018, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI87082354, Length=472, Percent_Identity=27.5423728813559, Blast_Score=181, Evalue=9e-47,
Organism=Escherichia coli, GI1789915, Length=439, Percent_Identity=27.1070615034169, Blast_Score=145, Evalue=7e-36,
Organism=Escherichia coli, GI87081717, Length=455, Percent_Identity=24.1758241758242, Blast_Score=131, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI32565766, Length=466, Percent_Identity=36.480686695279, Blast_Score=283, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI17557007, Length=475, Percent_Identity=29.2631578947368, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI71983419, Length=463, Percent_Identity=27.8617710583153, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI71983429, Length=463, Percent_Identity=27.8617710583153, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI71982272, Length=480, Percent_Identity=24.375, Blast_Score=89, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6321091, Length=476, Percent_Identity=36.7647058823529, Blast_Score=275, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6325240, Length=474, Percent_Identity=27.8481012658228, Blast_Score=176, Evalue=8e-45,
Organism=Saccharomyces cerevisiae, GI6325166, Length=466, Percent_Identity=28.1115879828326, Blast_Score=159, Evalue=1e-39,
Organism=Drosophila melanogaster, GI21358499, Length=467, Percent_Identity=37.9014989293362, Blast_Score=295, Evalue=3e-80,
Organism=Drosophila melanogaster, GI24640553, Length=470, Percent_Identity=27.2340425531915, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24640549, Length=470, Percent_Identity=27.2340425531915, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24640551, Length=470, Percent_Identity=27.2340425531915, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI17737741, Length=473, Percent_Identity=25.5813953488372, Blast_Score=116, Evalue=4e-26,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49770; Mature: 49639

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKSFDLIVIGAGPGGYVAAIRGAQLGMSVAIVEREHLGGICLNWGCIPTKAMLRSSEV
CCCCCCCEEEEECCCCCEEEEEECHHCCCEEEEEEHHCCCCEEEECCCCCHHHHHHHHHH
FHLMQRAKEFGLKAEKVDYDLDAVVKRSRKVAGQLSGGIGHLMKKNKVTVFMGEAKLAGK
HHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEECEEECCC
GKVSVKTDKGAEDLTAKNIVLATGARARTLPGLEADGDLVWTYKHALEPKHMPKKLLVIG
CEEEEECCCCCCHHCCCCEEEECCCCCCCCCCCCCCCCEEEEEHHHCCCCCCCCEEEEEE
SGAIGIEFASFYNTLGADTTVVEVMDRVLPVEDEEISAFAKKAFEKQGMKIMQKAMVKQL
CCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRAKGKVTAHIEVGGKVEKHEFDTVISAVGIVGNVEGLGLEELGVKIDRSHVVTDEFCRT
HHCCCCEEEEEEECCEECHHHHHHHHHHHHHHCCCCCCCHHHHCCEECCCCCCHHHHHHH
GIEGLYAIGDVAGAPWLAHKASHEGVMVADLIAGKHAHPVKPETIAGCTYCHPQVASVGY
HHHHHHHHHHHCCCCHHHHCCCCCCEEEEHHHCCCCCCCCCCCCCCCCCCCCCHHHHCCC
SEAKAKELGYTVKVGRFPFIGNGKAIALGEPEGMIKTVFDAKTGELLGAHMVGAEVTELI
HHHHHHHCCCEEEECCEEEECCCCEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHH
QGYVVGRQLETTEEDLMNTVFPHPTLSEMMHESVLDAYDRVIHM
HHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ASKSFDLIVIGAGPGGYVAAIRGAQLGMSVAIVEREHLGGICLNWGCIPTKAMLRSSEV
CCCCCCEEEEECCCCCEEEEEECHHCCCEEEEEEHHCCCCEEEECCCCCHHHHHHHHHH
FHLMQRAKEFGLKAEKVDYDLDAVVKRSRKVAGQLSGGIGHLMKKNKVTVFMGEAKLAGK
HHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEECEEECCC
GKVSVKTDKGAEDLTAKNIVLATGARARTLPGLEADGDLVWTYKHALEPKHMPKKLLVIG
CEEEEECCCCCCHHCCCCEEEECCCCCCCCCCCCCCCCEEEEEHHHCCCCCCCCEEEEEE
SGAIGIEFASFYNTLGADTTVVEVMDRVLPVEDEEISAFAKKAFEKQGMKIMQKAMVKQL
CCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRAKGKVTAHIEVGGKVEKHEFDTVISAVGIVGNVEGLGLEELGVKIDRSHVVTDEFCRT
HHCCCCEEEEEEECCEECHHHHHHHHHHHHHHCCCCCCCHHHHCCEECCCCCCHHHHHHH
GIEGLYAIGDVAGAPWLAHKASHEGVMVADLIAGKHAHPVKPETIAGCTYCHPQVASVGY
HHHHHHHHHHHCCCCHHHHCCCCCCEEEEHHHCCCCCCCCCCCCCCCCCCCCCHHHHCCC
SEAKAKELGYTVKVGRFPFIGNGKAIALGEPEGMIKTVFDAKTGELLGAHMVGAEVTELI
HHHHHHHCCCEEEECCEEEECCCCEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHH
QGYVVGRQLETTEEDLMNTVFPHPTLSEMMHESVLDAYDRVIHM
HHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]