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The map label for this gene is opuAB [H]

Identifier: 110680132

GI number: 110680132

Start: 2809700

End: 2810734

Strand: Direct

Name: opuAB [H]

Synonym: RD1_2931

Alternate gene names: 110680132

Gene position: 2809700-2810734 (Clockwise)

Preceding gene: 110680131

Following gene: 110680133

Centisome position: 67.98

GC content: 58.65

Gene sequence:

>1035_bases
ATGGCGTATTACGACGGTCTGTTTGATAGGTTGGGACTGCGTGACTGGTGCGATCAAAGCGTTGAAACCGCGCCCACGTC
GATGCAGGCTTTGCTCGGCCAATTGGGCGGGGGTGACACGGAACAGACGATAGCGCGTATCCCCTTCCCCTCCCTTGATA
CGCTGCATGACTCCTGCGCCCAGATCCCGCAGACCCGCGATATGACCGCCGGGGTGGAGAATACGTTTCTGGCCGCCCGC
GAAAGCCTCAAACTGGTGCTGGACCCGATCACGCAACCGCTTTCATGGATGCTGGATGGCGCCTTGTGGCTATTTACCAC
CACGCCGTGGTTCATCATCGTGCCCCTGCTTGTGGCCCTGACATGGTATGTGGCGCGATCCATCGGCGTGACCCTGTTCG
TTGCCGTCTCTTTCCTGTTTCTGGGGTTCATTGACCATCTGGATGTGGCGTTGGAAACCCTGTCGATCATCTTTGTCTGC
ACCGGTTTGTCGGTTGCCCTAGGCGTGCCGATTGGTATCGCCATGTCACGCTCCAACACCCTGCAACGCATGATCGTGCC
CATCCTTGACCTGTTGCAGACCCTGCCCACCTTCGTCTATCTCATCCCGCTGATCTTTTTGTTTTCGGTCACGGAATCCA
AGCTCTACGGGATCGCGATCATCCTTTACGCCATTGTCCCGGTAATCCGCCTGACCGACCTAGGCATCCGGCTGGTCGAC
CCGGATGTGATCGAGGCCGCAGACGCCTTTGGCATGACAAAACGCCAGAAACTCTGGGGGGTGCAGTTGCCACTGGCGCT
TCCCAATATCATGGCGGGCGTCAATCAGACCATCATGATGAGCCTCGCGATGGTGGTGATTGCGTCGCTTGTCTCTGCCC
CGGGCCTTGGGGTGCTGGTGCTGCGCGGCATCCGCAATCTGGAACTTGGCGTCGGGATTGTCGCGGGTTTTGGCATCGTC
TTGCTGGCCGTCATCCTTGACCGCGCCTCCAAGGCGGCCTTGGGGCGCATCGACAAGAACAAGAATGCACATTGA

Upstream 100 bases:

>100_bases
CGCCAGGCTGAGCGCCCTGCTGCAATAACCCCGCAGCGGTACGAACAACCGTGCAGCGCCTCACGAACGGGCGCTGCACA
GGACATCAAAAGGGATTTGC

Downstream 100 bases:

>100_bases
GACCCGAAGCATGAAGCCTGAACCCAAAGTCAGATTGCGCGGCGTCTACAAGATTTTCGGCCCGCGCGACAAGCAGATGG
TGGAACGGGTGCAAAACGGT

Product: glycine betaine transporter, permease protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MAYYDGLFDRLGLRDWCDQSVETAPTSMQALLGQLGGGDTEQTIARIPFPSLDTLHDSCAQIPQTRDMTAGVENTFLAAR
ESLKLVLDPITQPLSWMLDGALWLFTTTPWFIIVPLLVALTWYVARSIGVTLFVAVSFLFLGFIDHLDVALETLSIIFVC
TGLSVALGVPIGIAMSRSNTLQRMIVPILDLLQTLPTFVYLIPLIFLFSVTESKLYGIAIILYAIVPVIRLTDLGIRLVD
PDVIEAADAFGMTKRQKLWGVQLPLALPNIMAGVNQTIMMSLAMVVIASLVSAPGLGVLVLRGIRNLELGVGIVAGFGIV
LLAVILDRASKAALGRIDKNKNAH

Sequences:

>Translated_344_residues
MAYYDGLFDRLGLRDWCDQSVETAPTSMQALLGQLGGGDTEQTIARIPFPSLDTLHDSCAQIPQTRDMTAGVENTFLAAR
ESLKLVLDPITQPLSWMLDGALWLFTTTPWFIIVPLLVALTWYVARSIGVTLFVAVSFLFLGFIDHLDVALETLSIIFVC
TGLSVALGVPIGIAMSRSNTLQRMIVPILDLLQTLPTFVYLIPLIFLFSVTESKLYGIAIILYAIVPVIRLTDLGIRLVD
PDVIEAADAFGMTKRQKLWGVQLPLALPNIMAGVNQTIMMSLAMVVIASLVSAPGLGVLVLRGIRNLELGVGIVAGFGIV
LLAVILDRASKAALGRIDKNKNAH
>Mature_343_residues
AYYDGLFDRLGLRDWCDQSVETAPTSMQALLGQLGGGDTEQTIARIPFPSLDTLHDSCAQIPQTRDMTAGVENTFLAARE
SLKLVLDPITQPLSWMLDGALWLFTTTPWFIIVPLLVALTWYVARSIGVTLFVAVSFLFLGFIDHLDVALETLSIIFVCT
GLSVALGVPIGIAMSRSNTLQRMIVPILDLLQTLPTFVYLIPLIFLFSVTESKLYGIAIILYAIVPVIRLTDLGIRLVDP
DVIEAADAFGMTKRQKLWGVQLPLALPNIMAGVNQTIMMSLAMVVIASLVSAPGLGVLVLRGIRNLELGVGIVAGFGIVL
LAVILDRASKAALGRIDKNKNAH

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=325, Percent_Identity=37.2307692307692, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1788451, Length=201, Percent_Identity=32.8358208955224, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1788449, Length=184, Percent_Identity=30.4347826086957, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786564, Length=166, Percent_Identity=32.5301204819277, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 37318; Mature: 37187

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYYDGLFDRLGLRDWCDQSVETAPTSMQALLGQLGGGDTEQTIARIPFPSLDTLHDSCA
CCCHHHHHHHCCCHHHHCCHHHCCCHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHH
QIPQTRDMTAGVENTFLAARESLKLVLDPITQPLSWMLDGALWLFTTTPWFIIVPLLVAL
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHH
TWYVARSIGVTLFVAVSFLFLGFIDHLDVALETLSIIFVCTGLSVALGVPIGIAMSRSNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCHHH
LQRMIVPILDLLQTLPTFVYLIPLIFLFSVTESKLYGIAIILYAIVPVIRLTDLGIRLVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECC
PDVIEAADAFGMTKRQKLWGVQLPLALPNIMAGVNQTIMMSLAMVVIASLVSAPGLGVLV
CHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHHH
LRGIRNLELGVGIVAGFGIVLLAVILDRASKAALGRIDKNKNAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AYYDGLFDRLGLRDWCDQSVETAPTSMQALLGQLGGGDTEQTIARIPFPSLDTLHDSCA
CCHHHHHHHCCCHHHHCCHHHCCCHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHH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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]