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The map label for this gene is purR [H]

Identifier: 110680125

GI number: 110680125

Start: 2799241

End: 2800278

Strand: Direct

Name: purR [H]

Synonym: RD1_2923

Alternate gene names: 110680125

Gene position: 2799241-2800278 (Clockwise)

Preceding gene: 110680118

Following gene: 110680131

Centisome position: 67.73

GC content: 58.96

Gene sequence:

>1038_bases
GTGTTCTATATGGCTGATAAAAATACGAAAAATGCCGATATCGTTGCTGTTGCAAATGCCGCGAAGGTCTCAATTTCCAC
CGTGTCCCGCAGTTTCAATCACCCCGAGTTGGTGAACCCTGCGACTCGCAAAAAGATCGACCGCGCGGTGCGCAAACTGG
GCTATATCCGCAACCGGGCCGCGCAAACCATGCATGGCATTCGCAGTGGTACGATCGGGCTTGTGCTGCCGACCATCGAC
AATGTGATTTTCGCAGAAGTGATACAGGCCTTTTCCGAAAGCGTGGATGCGCATGGGTTTACAATCCTTGTGGCCTCGCA
TGGCTATGACCTCGAACGCGAATATGCGGTGCTGCGCAAGTTTCTGGAACACCGCGTGGATGGCGTGGCGCTGATCGGGC
TGGATCACAGCGAAGAAACCTATCAGCTGATTGACAGTCAGGCCATTCCGGCCATCACCATATGGAACTATGACGCGGCC
TCGCGGCTCTCTTGTGTGGGGGCGGACAACTGGCAGGCGGGCCGTTTGGCCGCACAGCATCTCATTGATCTGGGACATCG
CGAAATCGCGGTGGTTTTTCCTCCTGTTGCGGGTAACGACCGGGCGCGCGCCAGACATGACGGGGCGATGTCCGTTTTGT
CCCGACACTGCTGCGACCTGCCCCCCGCCTGGACACAGCAGGCGCGCTACAGCATTGCCGATGGCAAAGAGGCGGTGATC
AACCTGCTGCGCGCAGACCGGCGCCCAACGGCCATTTTATGCGGCAATGACGTTCTGGCCGTGGGGGCGATTCATGCAGC
ACGCCGCCTCGATCTCGGGGTGCCAGAGGATTTGTCGATCATCGGCATCGGCGATTTCAAAGGGTCGAAGGAAATGGAAC
CGGCGCTGACCACCATCAGCCTGCCCGCGCGCGCCATCGGTGAAATCGCCGGGGAGCAGCTGGCCCAGTCCATTGTGCAG
GAAGACCACCAGATCCGCCGCACGGACTGCGCCATTTCGCTGGCAAAGCGTGACACATGTCAGGTCTGTACGACCTAG

Upstream 100 bases:

>100_bases
AACTCCATTCAGGCCTCCCCTCCCGTCAGGATGATCTTGACCTTCGAGGTTAGGGGATGAAAATAGTATTGCAAATGTTT
TCATAAACCGTGACGTTCGG

Downstream 100 bases:

>100_bases
CAGCGTTTCAAAAATCCGCGCACATCAGGCGCGCACGATCCGCATCCAGTCCCATGGACAGAAGCACCGGGGCGCTGCGC
TGGCGGCTCTGGAAGTCTTC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MFYMADKNTKNADIVAVANAAKVSISTVSRSFNHPELVNPATRKKIDRAVRKLGYIRNRAAQTMHGIRSGTIGLVLPTID
NVIFAEVIQAFSESVDAHGFTILVASHGYDLEREYAVLRKFLEHRVDGVALIGLDHSEETYQLIDSQAIPAITIWNYDAA
SRLSCVGADNWQAGRLAAQHLIDLGHREIAVVFPPVAGNDRARARHDGAMSVLSRHCCDLPPAWTQQARYSIADGKEAVI
NLLRADRRPTAILCGNDVLAVGAIHAARRLDLGVPEDLSIIGIGDFKGSKEMEPALTTISLPARAIGEIAGEQLAQSIVQ
EDHQIRRTDCAISLAKRDTCQVCTT

Sequences:

>Translated_345_residues
MFYMADKNTKNADIVAVANAAKVSISTVSRSFNHPELVNPATRKKIDRAVRKLGYIRNRAAQTMHGIRSGTIGLVLPTID
NVIFAEVIQAFSESVDAHGFTILVASHGYDLEREYAVLRKFLEHRVDGVALIGLDHSEETYQLIDSQAIPAITIWNYDAA
SRLSCVGADNWQAGRLAAQHLIDLGHREIAVVFPPVAGNDRARARHDGAMSVLSRHCCDLPPAWTQQARYSIADGKEAVI
NLLRADRRPTAILCGNDVLAVGAIHAARRLDLGVPEDLSIIGIGDFKGSKEMEPALTTISLPARAIGEIAGEQLAQSIVQ
EDHQIRRTDCAISLAKRDTCQVCTT
>Mature_345_residues
MFYMADKNTKNADIVAVANAAKVSISTVSRSFNHPELVNPATRKKIDRAVRKLGYIRNRAAQTMHGIRSGTIGLVLPTID
NVIFAEVIQAFSESVDAHGFTILVASHGYDLEREYAVLRKFLEHRVDGVALIGLDHSEETYQLIDSQAIPAITIWNYDAA
SRLSCVGADNWQAGRLAAQHLIDLGHREIAVVFPPVAGNDRARARHDGAMSVLSRHCCDLPPAWTQQARYSIADGKEAVI
NLLRADRRPTAILCGNDVLAVGAIHAARRLDLGVPEDLSIIGIGDFKGSKEMEPALTTISLPARAIGEIAGEQLAQSIVQ
EDHQIRRTDCAISLAKRDTCQVCTT

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=328, Percent_Identity=29.8780487804878, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI48994940, Length=324, Percent_Identity=31.7901234567901, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1790369, Length=308, Percent_Identity=30.1948051948052, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI1790194, Length=324, Percent_Identity=28.7037037037037, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1790715, Length=323, Percent_Identity=25.6965944272446, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1787906, Length=327, Percent_Identity=28.1345565749235, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1788474, Length=302, Percent_Identity=28.476821192053, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1786540, Length=337, Percent_Identity=28.7833827893175, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789068, Length=302, Percent_Identity=26.4900662251656, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1789202, Length=292, Percent_Identity=26.3698630136986, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787580, Length=309, Percent_Identity=23.3009708737864, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790689, Length=284, Percent_Identity=22.5352112676056, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37602; Mature: 37602

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYMADKNTKNADIVAVANAAKVSISTVSRSFNHPELVNPATRKKIDRAVRKLGYIRNRA
CEEECCCCCCCCEEEEEECCCEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AQTMHGIRSGTIGLVLPTIDNVIFAEVIQAFSESVDAHGFTILVASHGYDLEREYAVLRK
HHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHH
FLEHRVDGVALIGLDHSEETYQLIDSQAIPAITIWNYDAASRLSCVGADNWQAGRLAAQH
HHHHCCCCEEEEECCCCHHHHHHHHCCCCCEEEEECCCCHHHEEEECCCCCCHHHHHHHH
LIDLGHREIAVVFPPVAGNDRARARHDGAMSVLSRHCCDLPPAWTQQARYSIADGKEAVI
HHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHCEECCCCHHHHH
NLLRADRRPTAILCGNDVLAVGAIHAARRLDLGVPEDLSIIGIGDFKGSKEMEPALTTIS
HHHHCCCCCCEEEECCCEEEHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCHHEEEE
LPARAIGEIAGEQLAQSIVQEDHQIRRTDCAISLAKRDTCQVCTT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MFYMADKNTKNADIVAVANAAKVSISTVSRSFNHPELVNPATRKKIDRAVRKLGYIRNRA
CEEECCCCCCCCEEEEEECCCEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AQTMHGIRSGTIGLVLPTIDNVIFAEVIQAFSESVDAHGFTILVASHGYDLEREYAVLRK
HHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHH
FLEHRVDGVALIGLDHSEETYQLIDSQAIPAITIWNYDAASRLSCVGADNWQAGRLAAQH
HHHHCCCCEEEEECCCCHHHHHHHHCCCCCEEEEECCCCHHHEEEECCCCCCHHHHHHHH
LIDLGHREIAVVFPPVAGNDRARARHDGAMSVLSRHCCDLPPAWTQQARYSIADGKEAVI
HHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHCEECCCCHHHHH
NLLRADRRPTAILCGNDVLAVGAIHAARRLDLGVPEDLSIIGIGDFKGSKEMEPALTTIS
HHHHCCCCCCEEEECCCEEEHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCHHEEEE
LPARAIGEIAGEQLAQSIVQEDHQIRRTDCAISLAKRDTCQVCTT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA