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The map label for this gene is lcfB [H]

Identifier: 110680054

GI number: 110680054

Start: 2719557

End: 2721062

Strand: Reverse

Name: lcfB [H]

Synonym: RD1_2849

Alternate gene names: 110680054

Gene position: 2721062-2719557 (Counterclockwise)

Preceding gene: 110680058

Following gene: 110680053

Centisome position: 65.84

GC content: 62.02

Gene sequence:

>1506_bases
ATGAACCTCGCACATTGGCTTGAGCGACAGGCGCAGATCACGCCCGGGCGACCCGCGCTTTTTCTCGGTACAGAGTGTGT
GGCGGACTACGGCCAGTTCTGGCGCGATGCCCGCGCCGTGGCGGGCTGGCTTTTGGCGCAGGGTGTGGGGTCGGGTGACC
GCGTGGCGCTGTTCATGACGAATGTACCGGATTATCTGACCGTCTTTTACGGGATCTGGGCGGTCGGGGCGGCGGCGGTG
CCGATCAATGCCAAGTTACACGGCCGTGAAGCGGCGTTCATTCTTGAGAACAGCGGTGCCAAACATGTGTTCACCTCGCC
GGAGTTGAGCGCATCGCTCATTGAGGCCGGTGTCAGCACAACTCAGACCGAGGTGCCCGGCACCGCGTTCGAGGCCATCC
GGGCGCACGAGCCGATAGATGTAATTGTGCCACGCGACCCGGATGATCTGGCCTGGCTGTTTTATACGTCCGGGACAACG
GGCAAGCCCAAAGGGGTGATGATCACCCATCGCATGCTCAGCGTGATGAGCCTGTGTTACGTCACGGATATCGACCCGGT
TTCCCCGGAGGATGCCACGATCTACGCTGCGCCGATGAGCCATGGCGCAGGTATCTACAACATGATCCACGTCCGCGCCG
GGGCGCGCCACGTTTGCCCGCCCTCGGGGGGCTTTGATCCCGATGAAATCCTGACGCTGGCGCAGCATTTCGGGAATGCG
CATCTTTTTGCGGCCCCCACGATGGTCAACCGCCTGACCCAATATGCCTCAGGCGCAGGCCAGCGGGGCGAGGGGCTGCG
CACGATCATCTACGCGGGTGGGCCGATGTATAACGCGGATATCATCGCGGCCGTGGATCACTTCGGGCCTGTCTTCATAC
AGATCTATGGTCAGGGCGAATGCCCGATGGGTATCACCGCCCTGTCCCGCGCGGATGTGACAGACAGGACGCATGCCGAT
TGGGCGCGTCGCCTGCAATCGGTCGGGCGTGCGCAATCCGCGGTCGAGGTCGCGATTGGGGACGAAAACGGCAAGCCGCT
CACCGTCGGAGAACACGGAGAAATCATGGTGCGCGGCGACACGGTCATGCCCGGATATTGGACCAACCCGGAGGCGACGG
CCAAGACCCTGCTGAACGGCTGGCTGATGACGGGCGACATGGGGTTCATGTCAGCAGATGGATACCTGACCCTGCAGGAC
CGGTCCAAAGACATGATCATCACGGGGGGATCGAACGTCTACCCGCGCGAAGTCGAAGAGGTTTTGCTGCGCGACAGCGG
CGTCACCGAAGTGTCGGTCGTGGGACGCCCCCATGCGGAGTGGGGTGAGGAGGTCGTCGCATTCGTCGTTGGCGACGTGG
ACCCTGCGCGGCTGGATCAGCTGTGCCTTGATCATATGGCGCGCTTCAAGCGGCCGAAGGAATACGTCAAGCTAGAGGCC
TTGCCGAAAAACAATTACGGTAAAATCCTGAAAACGGAGTTGCGCGCGCTTTTGACCCGGTCGTGA

Upstream 100 bases:

>100_bases
CTTGCGCGCTCGTTATATGCGAAGGGTGACGCCATGGAAACCCGCCTATTGCAGCGCCGCGCAGAGCAGGTAAACTCCAA
TCCCGCAAAAGGAGCAAGGG

Downstream 100 bases:

>100_bases
CGGGCGGGATCTGGGAACAGATTCGAGGGATTGAGACAGAGGCGGTTCGGTGCATTCCGCCAGTCTGCCCAGTGGGGAGT
ACGTCAGGATGACTAAAATT

Product: AMP-binding domain-containing protein

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MNLAHWLERQAQITPGRPALFLGTECVADYGQFWRDARAVAGWLLAQGVGSGDRVALFMTNVPDYLTVFYGIWAVGAAAV
PINAKLHGREAAFILENSGAKHVFTSPELSASLIEAGVSTTQTEVPGTAFEAIRAHEPIDVIVPRDPDDLAWLFYTSGTT
GKPKGVMITHRMLSVMSLCYVTDIDPVSPEDATIYAAPMSHGAGIYNMIHVRAGARHVCPPSGGFDPDEILTLAQHFGNA
HLFAAPTMVNRLTQYASGAGQRGEGLRTIIYAGGPMYNADIIAAVDHFGPVFIQIYGQGECPMGITALSRADVTDRTHAD
WARRLQSVGRAQSAVEVAIGDENGKPLTVGEHGEIMVRGDTVMPGYWTNPEATAKTLLNGWLMTGDMGFMSADGYLTLQD
RSKDMIITGGSNVYPREVEEVLLRDSGVTEVSVVGRPHAEWGEEVVAFVVGDVDPARLDQLCLDHMARFKRPKEYVKLEA
LPKNNYGKILKTELRALLTRS

Sequences:

>Translated_501_residues
MNLAHWLERQAQITPGRPALFLGTECVADYGQFWRDARAVAGWLLAQGVGSGDRVALFMTNVPDYLTVFYGIWAVGAAAV
PINAKLHGREAAFILENSGAKHVFTSPELSASLIEAGVSTTQTEVPGTAFEAIRAHEPIDVIVPRDPDDLAWLFYTSGTT
GKPKGVMITHRMLSVMSLCYVTDIDPVSPEDATIYAAPMSHGAGIYNMIHVRAGARHVCPPSGGFDPDEILTLAQHFGNA
HLFAAPTMVNRLTQYASGAGQRGEGLRTIIYAGGPMYNADIIAAVDHFGPVFIQIYGQGECPMGITALSRADVTDRTHAD
WARRLQSVGRAQSAVEVAIGDENGKPLTVGEHGEIMVRGDTVMPGYWTNPEATAKTLLNGWLMTGDMGFMSADGYLTLQD
RSKDMIITGGSNVYPREVEEVLLRDSGVTEVSVVGRPHAEWGEEVVAFVVGDVDPARLDQLCLDHMARFKRPKEYVKLEA
LPKNNYGKILKTELRALLTRS
>Mature_501_residues
MNLAHWLERQAQITPGRPALFLGTECVADYGQFWRDARAVAGWLLAQGVGSGDRVALFMTNVPDYLTVFYGIWAVGAAAV
PINAKLHGREAAFILENSGAKHVFTSPELSASLIEAGVSTTQTEVPGTAFEAIRAHEPIDVIVPRDPDDLAWLFYTSGTT
GKPKGVMITHRMLSVMSLCYVTDIDPVSPEDATIYAAPMSHGAGIYNMIHVRAGARHVCPPSGGFDPDEILTLAQHFGNA
HLFAAPTMVNRLTQYASGAGQRGEGLRTIIYAGGPMYNADIIAAVDHFGPVFIQIYGQGECPMGITALSRADVTDRTHAD
WARRLQSVGRAQSAVEVAIGDENGKPLTVGEHGEIMVRGDTVMPGYWTNPEATAKTLLNGWLMTGDMGFMSADGYLTLQD
RSKDMIITGGSNVYPREVEEVLLRDSGVTEVSVVGRPHAEWGEEVVAFVVGDVDPARLDQLCLDHMARFKRPKEYVKLEA
LPKNNYGKILKTELRALLTRS

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=488, Percent_Identity=26.6393442622951, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI187761343, Length=488, Percent_Identity=26.6393442622951, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI156151445, Length=547, Percent_Identity=25.0457038391225, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI42544132, Length=541, Percent_Identity=25.6931608133087, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI38505220, Length=526, Percent_Identity=26.615969581749, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI157311624, Length=539, Percent_Identity=24.860853432282, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI157311622, Length=539, Percent_Identity=24.860853432282, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI58082049, Length=539, Percent_Identity=25.0463821892393, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI122937307, Length=524, Percent_Identity=24.8091603053435, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI115511026, Length=487, Percent_Identity=25.4620123203285, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI42794756, Length=544, Percent_Identity=23.5294117647059, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI42794760, Length=544, Percent_Identity=23.1617647058824, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI42794758, Length=544, Percent_Identity=23.1617647058824, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI13375727, Length=532, Percent_Identity=22.7443609022556, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1788107, Length=544, Percent_Identity=29.7794117647059, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI221142682, Length=489, Percent_Identity=27.19836400818, Blast_Score=175, Evalue=7e-45,
Organism=Escherichia coli, GI145693145, Length=527, Percent_Identity=26.5654648956357, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI1786810, Length=503, Percent_Identity=24.4532803180915, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788595, Length=465, Percent_Identity=23.6559139784946, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1786529, Length=572, Percent_Identity=24.8251748251748, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17558820, Length=485, Percent_Identity=27.8350515463918, Blast_Score=178, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI32563687, Length=498, Percent_Identity=27.3092369477912, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI71994690, Length=538, Percent_Identity=27.1375464684015, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI71994703, Length=538, Percent_Identity=27.1375464684015, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI71994694, Length=538, Percent_Identity=27.1375464684015, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17559526, Length=521, Percent_Identity=30.7101727447217, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17560308, Length=521, Percent_Identity=25.9117082533589, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17538037, Length=499, Percent_Identity=24.6492985971944, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17560140, Length=487, Percent_Identity=24.6406570841889, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17557194, Length=506, Percent_Identity=23.3201581027668, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17531443, Length=498, Percent_Identity=22.8915662650602, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI25141359, Length=451, Percent_Identity=25.0554323725055, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71996755, Length=478, Percent_Identity=26.3598326359833, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71985884, Length=387, Percent_Identity=25.8397932816537, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17564090, Length=206, Percent_Identity=28.1553398058252, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=496, Percent_Identity=23.3870967741935, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24581924, Length=523, Percent_Identity=26.7686424474187, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI21355181, Length=495, Percent_Identity=26.2626262626263, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI18859661, Length=511, Percent_Identity=26.4187866927593, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI20130357, Length=368, Percent_Identity=26.6304347826087, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24648260, Length=357, Percent_Identity=26.6106442577031, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21356947, Length=521, Percent_Identity=22.8406909788868, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI19922652, Length=326, Percent_Identity=27.6073619631902, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI21358303, Length=320, Percent_Identity=23.75, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24648257, Length=366, Percent_Identity=25.1366120218579, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24653035, Length=391, Percent_Identity=22.5063938618926, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24656500, Length=300, Percent_Identity=25.3333333333333, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21356441, Length=333, Percent_Identity=24.024024024024, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648255, Length=497, Percent_Identity=21.9315895372233, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI161076582, Length=395, Percent_Identity=22.5316455696203, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648253, Length=497, Percent_Identity=21.7303822937626, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281365686, Length=148, Percent_Identity=31.7567567567568, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 54369; Mature: 54369

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLAHWLERQAQITPGRPALFLGTECVADYGQFWRDARAVAGWLLAQGVGSGDRVALFMT
CCHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
NVPDYLTVFYGIWAVGAAAVPINAKLHGREAAFILENSGAKHVFTSPELSASLIEAGVST
CCCHHHHHHHHHHHHHHEEEEECCEECCCEEEEEEECCCCCEEECCCCHHHHHHHHCCCC
TQTEVPGTAFEAIRAHEPIDVIVPRDPDDLAWLFYTSGTTGKPKGVMITHRMLSVMSLCY
CCCCCCCHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCCCCCCEEHHHHHHHHHHHHH
VTDIDPVSPEDATIYAAPMSHGAGIYNMIHVRAGARHVCPPSGGFDPDEILTLAQHFGNA
HCCCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCE
HLFAAPTMVNRLTQYASGAGQRGEGLRTIIYAGGPMYNADIIAAVDHFGPVFIQIYGQGE
EEEECHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEECCHHEEEHHCCCCEEEEEEECCC
CPMGITALSRADVTDRTHADWARRLQSVGRAQSAVEVAIGDENGKPLTVGEHGEIMVRGD
CCCCHHECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEECCCCEEEEECC
TVMPGYWTNPEATAKTLLNGWLMTGDMGFMSADGYLTLQDRSKDMIITGGSNVYPREVEE
EECCCCCCCCHHHHHHHHHCEEEECCCCEEECCCEEEEECCCCCEEEECCCCCCHHHHHH
VLLRDSGVTEVSVVGRPHAEWGEEVVAFVVGDVDPARLDQLCLDHMARFKRPKEYVKLEA
HHHHCCCCCEEEEECCCCHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHEEEEE
LPKNNYGKILKTELRALLTRS
CCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLAHWLERQAQITPGRPALFLGTECVADYGQFWRDARAVAGWLLAQGVGSGDRVALFMT
CCHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
NVPDYLTVFYGIWAVGAAAVPINAKLHGREAAFILENSGAKHVFTSPELSASLIEAGVST
CCCHHHHHHHHHHHHHHEEEEECCEECCCEEEEEEECCCCCEEECCCCHHHHHHHHCCCC
TQTEVPGTAFEAIRAHEPIDVIVPRDPDDLAWLFYTSGTTGKPKGVMITHRMLSVMSLCY
CCCCCCCHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCCCCCCEEHHHHHHHHHHHHH
VTDIDPVSPEDATIYAAPMSHGAGIYNMIHVRAGARHVCPPSGGFDPDEILTLAQHFGNA
HCCCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCE
HLFAAPTMVNRLTQYASGAGQRGEGLRTIIYAGGPMYNADIIAAVDHFGPVFIQIYGQGE
EEEECHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEECCHHEEEHHCCCCEEEEEEECCC
CPMGITALSRADVTDRTHADWARRLQSVGRAQSAVEVAIGDENGKPLTVGEHGEIMVRGD
CCCCHHECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEECCCCEEEEECC
TVMPGYWTNPEATAKTLLNGWLMTGDMGFMSADGYLTLQDRSKDMIITGGSNVYPREVEE
EECCCCCCCCHHHHHHHHHCEEEECCCCEEECCCEEEEECCCCCEEEECCCCCCHHHHHH
VLLRDSGVTEVSVVGRPHAEWGEEVVAFVVGDVDPARLDQLCLDHMARFKRPKEYVKLEA
HHHHCCCCCEEEEECCCCHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHEEEEE
LPKNNYGKILKTELRALLTRS
CCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]