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The map label for this gene is xylF [H]

Identifier: 110679995

GI number: 110679995

Start: 2655442

End: 2656422

Strand: Direct

Name: xylF [H]

Synonym: RD1_2785

Alternate gene names: 110679995

Gene position: 2655442-2656422 (Clockwise)

Preceding gene: 110679994

Following gene: 110679996

Centisome position: 64.25

GC content: 61.37

Gene sequence:

>981_bases
ATGCCATTGGCCGTTAAATCGCTCATCATCCTCACCCTGCTGTGCCTTGGGCTCGCCGTCCTTGTGCAATGGCGGGCAAG
TGCGCGTGAAACTGCGGTGGAGGCGCAATATCCACCCTCCGGTCAGTTCATTGATGTGGACGGGACCAGAATTCACCTTA
AGGTATCCGGACAAGGCCCTGATATTGTTCTGATTCATGGGGCCAGCGGGAGCCTGCGCGATTTCACCTTTGATCTGGCT
CAACGGCTTGAAACCCGCTACCGCGTGATCGCTGTTGACCGCCCCGGTCTTGGCCGCTCCGAACGCCCGCCGGGGTATGG
CGGGGTGTGGAACATCGCAGCCGAACCCCCCGCGCTACAAGCACAATTGTTGCAACGCGCAACGGATCAGCTTGGCGTCA
AAAACCCCATCGTCGTCGGGCATTCCTATGGAGGTGCCATCGCGCTGGCCTGGGCGCTGGAGCGGCCCGAGGAGACCGCC
GCGCTGGTGTTGCTGGCCGCTGCGTCAAACCCATGGGAGGGCGAATTGGGCTGGCTCTACCAGATCAATTCGACGCGATT
GGGCAGTGCCTTTGTCATCCCGCTGATCACGGCCTTTACACCGCATGCCGTCATCGAAAACACGCTGAGTGAGATTTTCG
CACCGCAAGCCCCTCCGCCCGACTATATGCAGCATTTCGGGGCCGACATGACGCTGCGCCGCGCGACCCTGCGCGCCAAT
GCGCAACAGGTCAACGCGCTGAAACCCTATGTCACCGAGATGTCCAAACGCTATCACACGCTGACGCTGCCTGTGGAAAG
CCTGCACGGGACAGAGGACACGATCGTGCCGCTCACCACACATGCAACGCCACTGGCGCGTCAAATCAGCGGCGCAGTCC
TGACCAAGATGGAAGGCATCGGCCACATGCCTCATCACGCTGATCCGCAGGCTGCCGTCGCCGCGATTGAGCGCGCAGCA
GCGCGTGCCGGTTTGCGTTAA

Upstream 100 bases:

>100_bases
GACCCGTCGCGCGCGCCGCTGAACCGCTGGCGCAGCCATGCCCATCTGCTTTATGGCAACTGGTTGACGGAGATCTACCA
AGACACGCCCTATGACATTG

Downstream 100 bases:

>100_bases
GCCGATTAACATTCCATATAGACAGCATGACTGACCTGCGGAGGATTCATGGATCTGCCTTTTGACGGTGCCATCAGCGC
TTATTTCGAGAACGACGCGC

Product: hydrolase, putative

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: HMSH [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MPLAVKSLIILTLLCLGLAVLVQWRASARETAVEAQYPPSGQFIDVDGTRIHLKVSGQGPDIVLIHGASGSLRDFTFDLA
QRLETRYRVIAVDRPGLGRSERPPGYGGVWNIAAEPPALQAQLLQRATDQLGVKNPIVVGHSYGGAIALAWALERPEETA
ALVLLAAASNPWEGELGWLYQINSTRLGSAFVIPLITAFTPHAVIENTLSEIFAPQAPPPDYMQHFGADMTLRRATLRAN
AQQVNALKPYVTEMSKRYHTLTLPVESLHGTEDTIVPLTTHATPLARQISGAVLTKMEGIGHMPHHADPQAAVAAIERAA
ARAGLR

Sequences:

>Translated_326_residues
MPLAVKSLIILTLLCLGLAVLVQWRASARETAVEAQYPPSGQFIDVDGTRIHLKVSGQGPDIVLIHGASGSLRDFTFDLA
QRLETRYRVIAVDRPGLGRSERPPGYGGVWNIAAEPPALQAQLLQRATDQLGVKNPIVVGHSYGGAIALAWALERPEETA
ALVLLAAASNPWEGELGWLYQINSTRLGSAFVIPLITAFTPHAVIENTLSEIFAPQAPPPDYMQHFGADMTLRRATLRAN
AQQVNALKPYVTEMSKRYHTLTLPVESLHGTEDTIVPLTTHATPLARQISGAVLTKMEGIGHMPHHADPQAAVAAIERAA
ARAGLR
>Mature_325_residues
PLAVKSLIILTLLCLGLAVLVQWRASARETAVEAQYPPSGQFIDVDGTRIHLKVSGQGPDIVLIHGASGSLRDFTFDLAQ
RLETRYRVIAVDRPGLGRSERPPGYGGVWNIAAEPPALQAQLLQRATDQLGVKNPIVVGHSYGGAIALAWALERPEETAA
LVLLAAASNPWEGELGWLYQINSTRLGSAFVIPLITAFTPHAVIENTLSEIFAPQAPPPDYMQHFGADMTLRRATLRANA
QQVNALKPYVTEMSKRYHTLTLPVESLHGTEDTIVPLTTHATPLARQISGAVLTKMEGIGHMPHHADPQAAVAAIERAAA
RAGLR

Specific function: Conversion of 2-hydroxymuconic acid semialdehyde to 2- hydroxypent-2,4-dienoate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 35160; Mature: 35029

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLAVKSLIILTLLCLGLAVLVQWRASARETAVEAQYPPSGQFIDVDGTRIHLKVSGQGP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEECCCCEEEEEECCCCC
DIVLIHGASGSLRDFTFDLAQRLETRYRVIAVDRPGLGRSERPPGYGGVWNIAAEPPALQ
CEEEEECCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCHHHH
AQLLQRATDQLGVKNPIVVGHSYGGAIALAWALERPEETAALVLLAAASNPWEGELGWLY
HHHHHHHHHHCCCCCCEEEEECCCCEEEEEEECCCCCCCEEEEEEEECCCCCCCCCEEEE
QINSTRLGSAFVIPLITAFTPHAVIENTLSEIFAPQAPPPDYMQHFGADMTLRRATLRAN
EECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEHHHHHHCC
AQQVNALKPYVTEMSKRYHTLTLPVESLHGTEDTIVPLTTHATPLARQISGAVLTKMEGI
HHHHHHHHHHHHHHHHCEEEEEEEHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCC
GHMPHHADPQAAVAAIERAAARAGLR
CCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PLAVKSLIILTLLCLGLAVLVQWRASARETAVEAQYPPSGQFIDVDGTRIHLKVSGQGP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEECCCCEEEEEECCCCC
DIVLIHGASGSLRDFTFDLAQRLETRYRVIAVDRPGLGRSERPPGYGGVWNIAAEPPALQ
CEEEEECCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCHHHH
AQLLQRATDQLGVKNPIVVGHSYGGAIALAWALERPEETAALVLLAAASNPWEGELGWLY
HHHHHHHHHHCCCCCCEEEEECCCCEEEEEEECCCCCCCEEEEEEEECCCCCCCCCEEEE
QINSTRLGSAFVIPLITAFTPHAVIENTLSEIFAPQAPPPDYMQHFGADMTLRRATLRAN
EECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEHHHHHHCC
AQQVNALKPYVTEMSKRYHTLTLPVESLHGTEDTIVPLTTHATPLARQISGAVLTKMEGI
HHHHHHHHHHHHHHHHCEEEEEEEHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCC
GHMPHHADPQAAVAAIERAAARAGLR
CCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1791759 [H]