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The map label for this gene is rluE [H]

Identifier: 110679964

GI number: 110679964

Start: 2625558

End: 2626121

Strand: Direct

Name: rluE [H]

Synonym: RD1_2753

Alternate gene names: 110679964

Gene position: 2625558-2626121 (Clockwise)

Preceding gene: 110679963

Following gene: 110679969

Centisome position: 63.53

GC content: 64.18

Gene sequence:

>564_bases
ATGGCCCGCGTCATCCTGTTCAACAAACCCTTCGGGGTGCTGTCGCAATTCACCGACAAGGGCAGCGAAGGATCACCGCG
CCCGACGCTTTCGGCCTTCATCGACGCGCCAGGGTTTTACCCCGCCGGACGGCTTGACAGGGACAGCGAGGGGCTGATGG
TGCTGACCGATGACGGCAAGTTGCAGGCGCGCATCAGCAATCCCAAACACAAGATGACCAAGACCTACCTGGCACAGATC
GAAGGCACACCGGAGCCACAGCACATCGAGGCGCTGCGCGCGGGCGTTCGTCTGAAAGACGGCATGACCCGTCCCGCGCA
AGTTAGTGTCGCAACGCCGCCCGATACCCTGTGGGAGCGCAGCCCGCCCGTGCGGTTTCGCAAGACAGTGCCGGATGCGT
GGCTGCAGATATCCATATCGGAAGGGCGCAATCGGCAGGTCCGGCGGATGTGCGCGCATGTGGGCTTGCCCTGTCTGCGC
CTGATCCGGATGCGTATCGGGGATTGGGCGCTGAATGATCTGGTGCCGGGGGCCTGGCGGTTTGCAGCCCACAGCGGGCG
GTGA

Upstream 100 bases:

>100_bases
GCAGCTTTGGCGGCGGGGCGAATACATCCCGATGTCGCTGGACGAAGACCTTGCACGGGCGGGCGCTGTTGGGGTCACGA
CGCTTCGGCCTGCCCCGTAA

Downstream 100 bases:

>100_bases
TCACAGGCGTTCGTATTTCGCTTCCTGATCGACTGTCCAGCGCACGGCAATTTCAAAACCATCTTCGGTCTGGTGCTCTT
CCTCCACCAGTTCCTGTTCG

Product: RNA pseudouridylate synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MARVILFNKPFGVLSQFTDKGSEGSPRPTLSAFIDAPGFYPAGRLDRDSEGLMVLTDDGKLQARISNPKHKMTKTYLAQI
EGTPEPQHIEALRAGVRLKDGMTRPAQVSVATPPDTLWERSPPVRFRKTVPDAWLQISISEGRNRQVRRMCAHVGLPCLR
LIRMRIGDWALNDLVPGAWRFAAHSGR

Sequences:

>Translated_187_residues
MARVILFNKPFGVLSQFTDKGSEGSPRPTLSAFIDAPGFYPAGRLDRDSEGLMVLTDDGKLQARISNPKHKMTKTYLAQI
EGTPEPQHIEALRAGVRLKDGMTRPAQVSVATPPDTLWERSPPVRFRKTVPDAWLQISISEGRNRQVRRMCAHVGLPCLR
LIRMRIGDWALNDLVPGAWRFAAHSGR
>Mature_186_residues
ARVILFNKPFGVLSQFTDKGSEGSPRPTLSAFIDAPGFYPAGRLDRDSEGLMVLTDDGKLQARISNPKHKMTKTYLAQIE
GTPEPQHIEALRAGVRLKDGMTRPAQVSVATPPDTLWERSPPVRFRKTVPDAWLQISISEGRNRQVRRMCAHVGLPCLRL
IRMRIGDWALNDLVPGAWRFAAHSGR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=179, Percent_Identity=55.3072625698324, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1788510, Length=184, Percent_Identity=35.3260869565217, Blast_Score=97, Evalue=5e-22,
Organism=Escherichia coli, GI1790453, Length=178, Percent_Identity=30.8988764044944, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787524, Length=186, Percent_Identity=31.1827956989247, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 20882; Mature: 20751

Theoretical pI: Translated: 10.95; Mature: 10.95

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARVILFNKPFGVLSQFTDKGSEGSPRPTLSAFIDAPGFYPAGRLDRDSEGLMVLTDDGK
CCEEEEEECCHHHHHHHHCCCCCCCCCCCHHHEECCCCCCCCCCCCCCCCCEEEEECCCE
LQARISNPKHKMTKTYLAQIEGTPEPQHIEALRAGVRLKDGMTRPAQVSVATPPDTLWER
EEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHCC
SPPVRFRKTVPDAWLQISISEGRNRQVRRMCAHVGLPCLRLIRMRIGDWALNDLVPGAWR
CCCCCEECCCCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCHHHHHCCCCCEE
FAAHSGR
CCCCCCC
>Mature Secondary Structure 
ARVILFNKPFGVLSQFTDKGSEGSPRPTLSAFIDAPGFYPAGRLDRDSEGLMVLTDDGK
CEEEEEECCHHHHHHHHCCCCCCCCCCCHHHEECCCCCCCCCCCCCCCCCEEEEECCCE
LQARISNPKHKMTKTYLAQIEGTPEPQHIEALRAGVRLKDGMTRPAQVSVATPPDTLWER
EEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHCC
SPPVRFRKTVPDAWLQISISEGRNRQVRRMCAHVGLPCLRLIRMRIGDWALNDLVPGAWR
CCCCCEECCCCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCHHHHHCCCCCEE
FAAHSGR
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]