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The map label for this gene is garR [C]

Identifier: 110679962

GI number: 110679962

Start: 2622120

End: 2622992

Strand: Reverse

Name: garR [C]

Synonym: RD1_2751

Alternate gene names: 110679962

Gene position: 2622992-2622120 (Counterclockwise)

Preceding gene: 110679965

Following gene: 110679959

Centisome position: 63.46

GC content: 61.05

Gene sequence:

>873_bases
ATGGCCAAACTGGCATTTCTGGGACTGGGCGTGATGGGCGCGCCCATGGCAGGGCATCTGCAAAAAGCAGGGCATGATGT
GACCGTCTATAACAGGACCGCTGCAAAAGCCGAGGCCTGGGTCGCGGCGCATGGCGGGGCCATGGCACCCACACCGGCCA
AGGCTGCCGCAGGTGCTGATTTCGTGATGGCCTGTGTGGGCAACGACGATGATTTGCGCGCGGTTTGTCTTGGGCCGGAT
GGGGCGTTTGCGGGCCTGTCTGCAGGGGCGGTTTTCGTGGATCACACGACCGTGTCGGCCGCTGTCACGCGCGAGCTTTA
TGAGGCTGCGAATAAAGCACAGATCAGCTTTGTCGATGCGCCGATTTCAGGCGGGCAGGCGGGGGCGGAAAACGCGGTGC
TCTCGATCATGTGTGGCGGTGATGAGGGGGCTTATGCGCGCGCCTTGCCCATCATGGAGGTGTATTCGAAAATCTGCCGC
CGGATCGGGGAGAGCGGCGCGGGTCAGATGACGAAGATGTGCAACCAGATTGCCATTGCCGGTTTGGTGCAGGGCCTGTC
CGAAGCGCTGCATTTTGCTGAGAAAGCAGGGCTGGACGGGCGCGCGGTCGTTGAGGTCATCAGTCAGGGTGCCGCGGGCA
GTTGGCAGATGTCAAACCGCTATGAAACCATGCTGGACGATCATTTTGACCACGGCTTTGCAACGGATTGGATGCGCAAG
GATCTGGGTATCTGTCTGGACACGGCCGATGAAACGGGCGCCAGCCTGCCGGTCACGGCGCTGGTGGATCAGTTCTACAA
GGACGTTCAGAAAATGGGTGGCGGCCGCTGGGACACATCCAGCCTGTTCAAACGCCTGCAGGCCTTGGGGTAA

Upstream 100 bases:

>100_bases
TGACGCCCTCGTGTCGAAACCGGTTCGAATGAATTGACCCTAGACCTCTGACTGTTAGGTAGGAGGGCAAGGTAACGCAA
CACCCAAAAGGGGGCTTCAA

Downstream 100 bases:

>100_bases
CGGGGCCTCAGGGCGCGTTCCGGCGCGCCCTGAATGTATTTTTCGGGTGTCAGGGAATGATGCGGCTGTATTTGTTGCCG
GCCAGTGACGCCCCGGCAAA

Product: 3-hydroxyisobutyrate dehydrogenase, putative

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MAKLAFLGLGVMGAPMAGHLQKAGHDVTVYNRTAAKAEAWVAAHGGAMAPTPAKAAAGADFVMACVGNDDDLRAVCLGPD
GAFAGLSAGAVFVDHTTVSAAVTRELYEAANKAQISFVDAPISGGQAGAENAVLSIMCGGDEGAYARALPIMEVYSKICR
RIGESGAGQMTKMCNQIAIAGLVQGLSEALHFAEKAGLDGRAVVEVISQGAAGSWQMSNRYETMLDDHFDHGFATDWMRK
DLGICLDTADETGASLPVTALVDQFYKDVQKMGGGRWDTSSLFKRLQALG

Sequences:

>Translated_290_residues
MAKLAFLGLGVMGAPMAGHLQKAGHDVTVYNRTAAKAEAWVAAHGGAMAPTPAKAAAGADFVMACVGNDDDLRAVCLGPD
GAFAGLSAGAVFVDHTTVSAAVTRELYEAANKAQISFVDAPISGGQAGAENAVLSIMCGGDEGAYARALPIMEVYSKICR
RIGESGAGQMTKMCNQIAIAGLVQGLSEALHFAEKAGLDGRAVVEVISQGAAGSWQMSNRYETMLDDHFDHGFATDWMRK
DLGICLDTADETGASLPVTALVDQFYKDVQKMGGGRWDTSSLFKRLQALG
>Mature_289_residues
AKLAFLGLGVMGAPMAGHLQKAGHDVTVYNRTAAKAEAWVAAHGGAMAPTPAKAAAGADFVMACVGNDDDLRAVCLGPDG
AFAGLSAGAVFVDHTTVSAAVTRELYEAANKAQISFVDAPISGGQAGAENAVLSIMCGGDEGAYARALPIMEVYSKICRR
IGESGAGQMTKMCNQIAIAGLVQGLSEALHFAEKAGLDGRAVVEVISQGAAGSWQMSNRYETMLDDHFDHGFATDWMRKD
LGICLDTADETGASLPVTALVDQFYKDVQKMGGGRWDTSSLFKRLQALG

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=287, Percent_Identity=28.9198606271777, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI23308751, Length=291, Percent_Identity=28.1786941580756, Blast_Score=127, Evalue=1e-29,
Organism=Escherichia coli, GI145693186, Length=280, Percent_Identity=35.3571428571429, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1786719, Length=287, Percent_Identity=32.404181184669, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1790315, Length=288, Percent_Identity=30.2083333333333, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1789092, Length=226, Percent_Identity=27.4336283185841, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17557316, Length=276, Percent_Identity=28.2608695652174, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24655230, Length=300, Percent_Identity=28.3333333333333, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI19922568, Length=300, Percent_Identity=28.3333333333333, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI28574115, Length=282, Percent_Identity=24.113475177305, Blast_Score=100, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30167; Mature: 30035

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLAFLGLGVMGAPMAGHLQKAGHDVTVYNRTAAKAEAWVAAHGGAMAPTPAKAAAGAD
CCCCCEECCCHHCCCHHHHHHHCCCCEEEEECCHHHHHHHEEECCCCCCCCCCHHHCCCC
FVMACVGNDDDLRAVCLGPDGAFAGLSAGAVFVDHTTVSAAVTRELYEAANKAQISFVDA
EEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEEC
PISGGQAGAENAVLSIMCGGDEGAYARALPIMEVYSKICRRIGESGAGQMTKMCNQIAIA
CCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
GLVQGLSEALHFAEKAGLDGRAVVEVISQGAAGSWQMSNRYETMLDDHFDHGFATDWMRK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHH
DLGICLDTADETGASLPVTALVDQFYKDVQKMGGGRWDTSSLFKRLQALG
HHCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
AKLAFLGLGVMGAPMAGHLQKAGHDVTVYNRTAAKAEAWVAAHGGAMAPTPAKAAAGAD
CCCCEECCCHHCCCHHHHHHHCCCCEEEEECCHHHHHHHEEECCCCCCCCCCHHHCCCC
FVMACVGNDDDLRAVCLGPDGAFAGLSAGAVFVDHTTVSAAVTRELYEAANKAQISFVDA
EEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEEC
PISGGQAGAENAVLSIMCGGDEGAYARALPIMEVYSKICRRIGESGAGQMTKMCNQIAIA
CCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
GLVQGLSEALHFAEKAGLDGRAVVEVISQGAAGSWQMSNRYETMLDDHFDHGFATDWMRK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHH
DLGICLDTADETGASLPVTALVDQFYKDVQKMGGGRWDTSSLFKRLQALG
HHCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA