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The map label for this gene is nodE [H]

Identifier: 110679851

GI number: 110679851

Start: 2503289

End: 2504497

Strand: Direct

Name: nodE [H]

Synonym: RD1_2623

Alternate gene names: 110679851

Gene position: 2503289-2504497 (Clockwise)

Preceding gene: 110679850

Following gene: 110679853

Centisome position: 60.57

GC content: 60.46

Gene sequence:

>1209_bases
ATGAAACGTGTTGTGATCACCGGGGCAGGCACCATCAATGCCTTGGGCCATGACGTACCCACGACACTGGCCGCGATGCG
CGACGGTGTCTGTGGTATCGGACCCTTGGCCTTGCGTGATGTCGAGCGGCTGTCGATCCAGATCGGCGGGCAGGTGCGCG
ATTTTGAAGCCGAAGGGCGCTATAACCGTCAGCAGATGTCGCTTTATGACCGTTTCACGCAGTTCACACTGGCCGCCGCC
AATGAGGCGATCGCGCAATCCGGGCTGAGCTTTTCGGGCGCGCTTTCGGCCCGCTCCGGCGTTGTGCTGGGCACAGCGGG
CGGCGGTGTCAGCACGTGGGATGACAACTATCGCTCGGTCTATGAGGACGGAAAAAACCGGGTTCACCCCTTTGTCGTGC
CGAAATTGATGAACAATGCGGCCGCATCGCATGTGAGCATGGAACATAACCTCAAGGGTCCGTCGTTTACCGTATCGACG
GCCTGCGCCTCATCCAATCACGCCATGGCGCAGGCCTTTATGATGGTGAGATCCGGTTTGGCACCTGTCATGATCACTGG
CGGGTCGGAATCCATGCTCTGTTTTGGCGGCGTCAAGGCCTGGGAAGGGCTGCGGGTGATGTCGCGCGATGCCTGCCGCC
CGTTCTCGGCCAATCGTAACGGGATGGTGCAGGGCGAAGGGGCGGGAGTCTTTGTCTTTGAAGAGTATGACGCAGCCAAA
AAGCGCGGCGCAGATATCTTGTGCGAAGTCGTGGGGTTTGCCATGTCGTCTGATGCAGCCGATATCGTCATGCCCTCCAA
GAATGGTGCCGCACGCGCCATGGCCGGCGCGTTGAGCGATGCGGGGCTGAACCGGGACGAGGTGGGATACATCAATGCCC
ATGGCACAGGCACCGCTGCCAACGACAAGACCGAATGCGCTGCTGTCGCGGATGTCTTTGGGCCGCATGCGGACAAGTTG
ATGATATCCTCGACCAAATCAATGCACGGGCATCTGATCGGGGGCACGGGTGCTGTCGAACTGCTGGCCTGTATCATGGC
GGTTCGGGACGGTGTGATCGCCCCGACCATCGGCTATCAGGAACCCGATCCGGAATGTGCGCTCGATGTGGTGCCCAATG
AGGCGCGCGATGCCGATGTCACCGTCGCACTGTCCAACGCTTTTGCCTTTGGCGGCATGAACGCGGTGCTGGCCCTGCGC
AAGGTATGA

Upstream 100 bases:

>100_bases
CAACGCCAACAACCCGACCGAAAGTGATTTCGACATCTCCTCTGTCGCGTCCATCGTGGAAGGAATCACGCGCCTGCAGG
CAGAACAGGGCTGACGCTGC

Downstream 100 bases:

>100_bases
GCCGCGCTGCGCGCTCGCCTCTATTGCGTGAGGTAGGAGGATGCCTCGAACGCGGCGGTAAACCCTTTCAGTGACAAGGT
CACATTGACACGCTGATCGG

Product: beta-ketoacyl synthase family protein

Products: [acyl carrier protein]; acetoacetyl-ACP; CO2; [acyl carrier protein]; beta-ketoacyl-ACP [C]

Alternate protein names: Host-specificity of nodulation protein B [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKRVVITGAGTINALGHDVPTTLAAMRDGVCGIGPLALRDVERLSIQIGGQVRDFEAEGRYNRQQMSLYDRFTQFTLAAA
NEAIAQSGLSFSGALSARSGVVLGTAGGGVSTWDDNYRSVYEDGKNRVHPFVVPKLMNNAAASHVSMEHNLKGPSFTVST
ACASSNHAMAQAFMMVRSGLAPVMITGGSESMLCFGGVKAWEGLRVMSRDACRPFSANRNGMVQGEGAGVFVFEEYDAAK
KRGADILCEVVGFAMSSDAADIVMPSKNGAARAMAGALSDAGLNRDEVGYINAHGTGTAANDKTECAAVADVFGPHADKL
MISSTKSMHGHLIGGTGAVELLACIMAVRDGVIAPTIGYQEPDPECALDVVPNEARDADVTVALSNAFAFGGMNAVLALR
KV

Sequences:

>Translated_402_residues
MKRVVITGAGTINALGHDVPTTLAAMRDGVCGIGPLALRDVERLSIQIGGQVRDFEAEGRYNRQQMSLYDRFTQFTLAAA
NEAIAQSGLSFSGALSARSGVVLGTAGGGVSTWDDNYRSVYEDGKNRVHPFVVPKLMNNAAASHVSMEHNLKGPSFTVST
ACASSNHAMAQAFMMVRSGLAPVMITGGSESMLCFGGVKAWEGLRVMSRDACRPFSANRNGMVQGEGAGVFVFEEYDAAK
KRGADILCEVVGFAMSSDAADIVMPSKNGAARAMAGALSDAGLNRDEVGYINAHGTGTAANDKTECAAVADVFGPHADKL
MISSTKSMHGHLIGGTGAVELLACIMAVRDGVIAPTIGYQEPDPECALDVVPNEARDADVTVALSNAFAFGGMNAVLALR
KV
>Mature_402_residues
MKRVVITGAGTINALGHDVPTTLAAMRDGVCGIGPLALRDVERLSIQIGGQVRDFEAEGRYNRQQMSLYDRFTQFTLAAA
NEAIAQSGLSFSGALSARSGVVLGTAGGGVSTWDDNYRSVYEDGKNRVHPFVVPKLMNNAAASHVSMEHNLKGPSFTVST
ACASSNHAMAQAFMMVRSGLAPVMITGGSESMLCFGGVKAWEGLRVMSRDACRPFSANRNGMVQGEGAGVFVFEEYDAAK
KRGADILCEVVGFAMSSDAADIVMPSKNGAARAMAGALSDAGLNRDEVGYINAHGTGTAANDKTECAAVADVFGPHADKL
MISSTKSMHGHLIGGTGAVELLACIMAVRDGVIAPTIGYQEPDPECALDVVPNEARDADVTVALSNAFAFGGMNAVLALR
KV

Specific function: Proposed to synthesize NOD factor fatty acyl chain. Involved in the synthesis of a highly unsaturated fatty acid moiety, which forms part of a lipo-oligosaccharide that is responsible for host specificity [H]

COG id: COG0304

COG function: function code IQ; 3-oxoacyl-(acyl-carrier-protein) synthase

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-ketoacyl-ACP synthases family [H]

Homologues:

Organism=Homo sapiens, GI8923559, Length=415, Percent_Identity=35.421686746988, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI223972627, Length=162, Percent_Identity=38.2716049382716, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI41872631, Length=350, Percent_Identity=26, Blast_Score=99, Evalue=8e-21,
Organism=Escherichia coli, GI1787337, Length=410, Percent_Identity=39.2682926829268, Blast_Score=272, Evalue=2e-74,
Organism=Escherichia coli, GI1788663, Length=415, Percent_Identity=32.5301204819277, Blast_Score=191, Evalue=9e-50,
Organism=Caenorhabditis elegans, GI71983541, Length=412, Percent_Identity=32.5242718446602, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI71983535, Length=262, Percent_Identity=33.969465648855, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI212642053, Length=355, Percent_Identity=26.4788732394366, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17550940, Length=232, Percent_Identity=25.8620689655172, Blast_Score=95, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6320904, Length=444, Percent_Identity=32.6576576576577, Blast_Score=199, Evalue=9e-52,
Organism=Drosophila melanogaster, GI24644341, Length=417, Percent_Identity=34.5323741007194, Blast_Score=230, Evalue=1e-60,
Organism=Drosophila melanogaster, GI19920632, Length=379, Percent_Identity=27.1767810026385, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI221330659, Length=379, Percent_Identity=27.1767810026385, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24581345, Length=353, Percent_Identity=27.4787535410765, Blast_Score=109, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000794
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615 [H]

Pfam domain/function: PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C [H]

EC number: 2.3.1.41 [C]

Molecular weight: Translated: 41963; Mature: 41963

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00098 THIOLASE_1 ; PS00606 B_KETOACYL_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVVITGAGTINALGHDVPTTLAAMRDGVCGIGPLALRDVERLSIQIGGQVRDFEAEGR
CCEEEEECCCCHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHEEEEECCEEEEECCCCC
YNRQQMSLYDRFTQFTLAAANEAIAQSGLSFSGALSARSGVVLGTAGGGVSTWDDNYRSV
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHH
YEDGKNRVHPFVVPKLMNNAAASHVSMEHNLKGPSFTVSTACASSNHAMAQAFMMVRSGL
HHCCCCCCCCEEHHHHHHHHHHHHEEHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCC
APVMITGGSESMLCFGGVKAWEGLRVMSRDACRPFSANRNGMVQGEGAGVFVFEEYDAAK
CCEEEECCCCCEEEECCCHHHCCCHHHHHCCCCCCCCCCCCEEEECCCEEEEECCCCHHH
KRGADILCEVVGFAMSSDAADIVMPSKNGAARAMAGALSDAGLNRDEVGYINAHGTGTAA
HCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCC
NDKTECAAVADVFGPHADKLMISSTKSMHGHLIGGTGAVELLACIMAVRDGVIAPTIGYQ
CCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEECCCCHHHHHHHHHHHHCCCEECCCCCC
EPDPECALDVVPNEARDADVTVALSNAFAFGGMNAVLALRKV
CCCCCCEEEECCCCCCCCEEEEEEECCHHHCCHHHHHHEECC
>Mature Secondary Structure
MKRVVITGAGTINALGHDVPTTLAAMRDGVCGIGPLALRDVERLSIQIGGQVRDFEAEGR
CCEEEEECCCCHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHEEEEECCEEEEECCCCC
YNRQQMSLYDRFTQFTLAAANEAIAQSGLSFSGALSARSGVVLGTAGGGVSTWDDNYRSV
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHH
YEDGKNRVHPFVVPKLMNNAAASHVSMEHNLKGPSFTVSTACASSNHAMAQAFMMVRSGL
HHCCCCCCCCEEHHHHHHHHHHHHEEHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCC
APVMITGGSESMLCFGGVKAWEGLRVMSRDACRPFSANRNGMVQGEGAGVFVFEEYDAAK
CCEEEECCCCCEEEECCCHHHCCCHHHHHCCCCCCCCCCCCEEEECCCEEEEECCCCHHH
KRGADILCEVVGFAMSSDAADIVMPSKNGAARAMAGALSDAGLNRDEVGYINAHGTGTAA
HCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCC
NDKTECAAVADVFGPHADKLMISSTKSMHGHLIGGTGAVELLACIMAVRDGVIAPTIGYQ
CCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEECCCCHHHHHHHHHHHHCCCEECCCCCC
EPDPECALDVVPNEARDADVTVALSNAFAFGGMNAVLALRKV
CCCCCCEEEECCCCCCCCEEEEEEECCHHHCCHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: None [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} [C]

Substrates: acetyl-ACP; malonyl-ACP; acyl-ACP [C]

Specific reaction: acetyl-ACP + malonyl-ACP = [acyl carrier protein] + acetoacetyl-ACP + CO2 acyl-ACP + malonyl-ACP = [acyl carrier protein] + beta-ketoacyl-ACP + CO2 [C]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl-ACP-decarboxylation, Cerulenin [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9100384 [H]