Click here to switch to the map view.

The map label for this gene is gluQ [H]

Identifier: 110679837

GI number: 110679837

Start: 2491583

End: 2492446

Strand: Reverse

Name: gluQ [H]

Synonym: RD1_2608

Alternate gene names: 110679837

Gene position: 2492446-2491583 (Counterclockwise)

Preceding gene: 110679838

Following gene: 110679835

Centisome position: 60.3

GC content: 63.08

Gene sequence:

>864_bases
GTGACATTCACAACCCGGTTTGCTCCATCCCCAACTGGCCCTCTGCATCTGGGGCATGCCTATTCCGCGCTTCTGGCCTG
GGATATGGCGGCGGCTGCGGGGGGCGCTTTCCTGCTCAGGATTGAGGATATTGACCAATCCCGCGCGCGACCGACCTGGG
AAGCGCAGATCTATAAGGACCTTGCGTGGTTGGGCCTGCGCTGGCCCGAGCCTGTTATGCGCCAGTCGGACCGCTTTGAT
GTTTATCGGGCTGCGCTGACGCGGCTCGAAAGGCTGGGCGTGACGTATCCCTGCACCTGTTCACGCAAGGACATCCAACT
GGCCGCCAGCGCGCCACAAGAAGGAAGCACGCCAGTTTTTGGACCTGACGGGCTGGTCTATCCCGGCACCTGCCGCGCAG
GGAGCCAGGCTGTCAGTGGGCCCCCCGCGCCCGCGATACGGCTGAATATGGCCAAGGCGCTGGATCTGATCGCGGACAGC
CCCCTTACCTATCAGGAGACCGGATCAGGGACGCATAAGGTGGTTTCAATTGAGCCCGCGACCGCGTTGACGCGCATCGG
GGATATCGTTCTGGCGCGCCGCGATATGGGCACCTCCTATCACCTGTCGGTGGTCGTCGATGATTCCGCCCAGAATGTCA
GCACCGTCGTGCGCGGCGCAGATCTGGCCGAGGCGACGCAGATCCACGTGATCCTGCAACGCCTGCTTGATGTGCCGACA
CCCGACTATTACCACCATCGACTGATCCGCGACGATGCCGGCAAGCGGTTGGCCAAACGTGATGATGCGCGGGCTTTGGC
AAAATTCCGCGACGAGGGGTGCGCGCCGCAGGACATCAGGGCCATGGTCGGGCTTGCGGTCTAG

Upstream 100 bases:

>100_bases
AATGGCTCGATTGTGGTGCCGCACGGCTCTTGTTCAAGGAACACGGGCCGCATTTGCCCGGGATCGACTTGAAGGCCACC
GTTTATGTCATTGAGAAAAC

Downstream 100 bases:

>100_bases
TCCTGCGGGGTCATCAGTTCCTGTTCATGCCCGTCACGCACCCCGGTGTAAAAACAGCTGCGACGCCCGGTGTGGCAGGC
AGGACCCGTCTGACGCAGCA

Product: glutamyl-tRNA synthetase, putative

Products: AMP; L-Glutamyl-tRNA(Glu); Diphosphate [C]

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MTFTTRFAPSPTGPLHLGHAYSALLAWDMAAAAGGAFLLRIEDIDQSRARPTWEAQIYKDLAWLGLRWPEPVMRQSDRFD
VYRAALTRLERLGVTYPCTCSRKDIQLAASAPQEGSTPVFGPDGLVYPGTCRAGSQAVSGPPAPAIRLNMAKALDLIADS
PLTYQETGSGTHKVVSIEPATALTRIGDIVLARRDMGTSYHLSVVVDDSAQNVSTVVRGADLAEATQIHVILQRLLDVPT
PDYYHHRLIRDDAGKRLAKRDDARALAKFRDEGCAPQDIRAMVGLAV

Sequences:

>Translated_287_residues
MTFTTRFAPSPTGPLHLGHAYSALLAWDMAAAAGGAFLLRIEDIDQSRARPTWEAQIYKDLAWLGLRWPEPVMRQSDRFD
VYRAALTRLERLGVTYPCTCSRKDIQLAASAPQEGSTPVFGPDGLVYPGTCRAGSQAVSGPPAPAIRLNMAKALDLIADS
PLTYQETGSGTHKVVSIEPATALTRIGDIVLARRDMGTSYHLSVVVDDSAQNVSTVVRGADLAEATQIHVILQRLLDVPT
PDYYHHRLIRDDAGKRLAKRDDARALAKFRDEGCAPQDIRAMVGLAV
>Mature_286_residues
TFTTRFAPSPTGPLHLGHAYSALLAWDMAAAAGGAFLLRIEDIDQSRARPTWEAQIYKDLAWLGLRWPEPVMRQSDRFDV
YRAALTRLERLGVTYPCTCSRKDIQLAASAPQEGSTPVFGPDGLVYPGTCRAGSQAVSGPPAPAIRLNMAKALDLIADSP
LTYQETGSGTHKVVSIEPATALTRIGDIVLARRDMGTSYHLSVVVDDSAQNVSTVVRGADLAEATQIHVILQRLLDVPTP
DYYHHRLIRDDAGKRLAKRDDARALAKFRDEGCAPQDIRAMVGLAV

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=289, Percent_Identity=32.8719723183391, Blast_Score=129, Evalue=3e-30,
Organism=Escherichia coli, GI1788743, Length=278, Percent_Identity=31.6546762589928, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI226510925, Length=266, Percent_Identity=35.3383458646617, Blast_Score=136, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71993394, Length=290, Percent_Identity=29.3103448275862, Blast_Score=110, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6324540, Length=284, Percent_Identity=28.5211267605634, Blast_Score=124, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24665321, Length=293, Percent_Identity=30.7167235494881, Blast_Score=114, Evalue=6e-26,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: 6.1.1.17 [C]

Molecular weight: Translated: 31232; Mature: 31101

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFTTRFAPSPTGPLHLGHAYSALLAWDMAAAAGGAFLLRIEDIDQSRARPTWEAQIYKD
CCEECCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHCCCCHHHHHHHH
LAWLGLRWPEPVMRQSDRFDVYRAALTRLERLGVTYPCTCSRKDIQLAASAPQEGSTPVF
HHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCC
GPDGLVYPGTCRAGSQAVSGPPAPAIRLNMAKALDLIADSPLTYQETGSGTHKVVSIEPA
CCCCEECCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCEECCCCCCEEEEEECCH
TALTRIGDIVLARRDMGTSYHLSVVVDDSAQNVSTVVRGADLAEATQIHVILQRLLDVPT
HHHHHHHHHHEECCCCCCEEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
PDYYHHRLIRDDAGKRLAKRDDARALAKFRDEGCAPQDIRAMVGLAV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TFTTRFAPSPTGPLHLGHAYSALLAWDMAAAAGGAFLLRIEDIDQSRARPTWEAQIYKD
CEECCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHCCCCHHHHHHHH
LAWLGLRWPEPVMRQSDRFDVYRAALTRLERLGVTYPCTCSRKDIQLAASAPQEGSTPVF
HHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCC
GPDGLVYPGTCRAGSQAVSGPPAPAIRLNMAKALDLIADSPLTYQETGSGTHKVVSIEPA
CCCCEECCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCEECCCCCCEEEEEECCH
TALTRIGDIVLARRDMGTSYHLSVVVDDSAQNVSTVVRGADLAEATQIHVILQRLLDVPT
HHHHHHHHHHEECCCCCCEEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
PDYYHHRLIRDDAGKRLAKRDDARALAKFRDEGCAPQDIRAMVGLAV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-Glutamate; tRNA (Glu) [C]

Specific reaction: ATP + L-Glutamate + tRNA (Glu) --> AMP + L-Glutamyl-tRNA(Glu) + Diphosphate [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]