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The map label for this gene is ecfE [C]

Identifier: 110679821

GI number: 110679821

Start: 2474140

End: 2475483

Strand: Direct

Name: ecfE [C]

Synonym: RD1_2591

Alternate gene names: 110679821

Gene position: 2474140-2475483 (Clockwise)

Preceding gene: 110679820

Following gene: 110679822

Centisome position: 59.86

GC content: 60.12

Gene sequence:

>1344_bases
ATGACCTTGATCCCGCAGTTTGGCGGGCTGATCTGGACGATTGCGGCCTTCATTCTGGCCTTGTCGGTGATCGTGGCCAT
CCACGAATACGGGCATTACATCGTCGGGCGCTGGTCGGGTATCCATGCAGATGTTTTTTCCATCGGCTTCGGGCCTGTGC
TTTTTGCGCGCACGGACAAACGGGGCACGCGCTGGCAGATTGCGGCCCTGCCTTTTGGCGGATACGTGAAATTTGCAGGG
GACGCCGATGCCGCCTCCGGCAAAGACGACGCGGCGATGGCCGAGGTTCAGAACGACCCGGTAAGGTTGCGGGCTACCAT
GCATGGTGCGCCGCTTTGGGCACGCACGGCCACGGTGGCGGCAGGTCCGATCTTCAATTTTGTACTGTCGATCATTGTCT
TTGCTGCCGTATTGCTGACAAGCGGTATCGCGCGTGATCCTTTGACCGTTGGCGAGATGCGGGCGCTGCCTGTGGAGGCG
GTCGGCTTGCAATCGGGCGATGAAATTCTCGGCATCAATGGAGTGGATATCCCCAGTACCGAGGACCGGGATGCCTATCG
CGCCTTTATCGAGGCGCTGCCGTTCGAGCCGGTGTTGACCTATGACGTGCTGCGGGATGGGCGCACGGTCACTGTTGATG
GGCCTTACATGTTGCCGCCGCTGGTCAATTCATTGACGCCACAAAGTGCGGCGATCCGGGCGGGCATGGCGCAGGGCGAT
GTGATCATCGCGATCAATGGCATCCCGATCTATGCCTTTGACGAATTGAAAAACGCGGTCGAAGGAGGCAATGGCGCAAC
GCTGGACCTCACCGTCTGGCGCGCGGGAGAGACGCTGGAGGTATCGCTGACGCCCAAACGGGTGGATGAGCCTCAGGATG
ACGGTGGTTTCGCCACGCAATGGCGTATTGGTATTGCCGGTGGGCTTGCCTTTGAGCCGGCAACAGAGCGTCCCGGGGTC
ATCGAGGCAATTTCCGGCGGTGTGAGCCAGACCTGGCGCATCATCAACGGCTCGCTATCAGGTCTGGGACATATGATCAG
CGGCGCGATCAGCACCTGCAATCTGAGCGGACCGATCGGGATCGCGCAAACCTCGGGTGCCATGGCCAGTCAGGGGGCGG
AGAGTTTCATCTGGTTCATTGCCGTTCTCAGCACCGCCGTGGGGCTGTTGAACCTTTTCCCTGTGCCTGCGCTGGATGGG
GGGCACCTTGTGTTTTACGCTTATGAGGCGGTGACCGGCAAGCCGCCCAGTGACAAGGCGCTACGCGTGCTGATGACATT
TGGGCTGGCAACGGTGCTGACGTTGATGCTCTTTGCGCTTGGCAATGATATCTTCTGCCCCTGA

Upstream 100 bases:

>100_bases
ACGCGGCCATGACCCTTGATAACGTGGCCCGGATAGACCATCTCGCCCGGGAAGAGGCGCGCAGGTTTATCAACAAGAGA
GCAGGCTGACATTGGATATT

Downstream 100 bases:

>100_bases
GGTGGGGATGCGTGACAGATGGTCCCGATCCTGCCCGCGTTGACCAAATTCTTGCCATATTCCAATGCTACCCAAGCCTC
ACACAGCGAGTGAGGGCATT

Product: protease ecfE, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MTLIPQFGGLIWTIAAFILALSVIVAIHEYGHYIVGRWSGIHADVFSIGFGPVLFARTDKRGTRWQIAALPFGGYVKFAG
DADAASGKDDAAMAEVQNDPVRLRATMHGAPLWARTATVAAGPIFNFVLSIIVFAAVLLTSGIARDPLTVGEMRALPVEA
VGLQSGDEILGINGVDIPSTEDRDAYRAFIEALPFEPVLTYDVLRDGRTVTVDGPYMLPPLVNSLTPQSAAIRAGMAQGD
VIIAINGIPIYAFDELKNAVEGGNGATLDLTVWRAGETLEVSLTPKRVDEPQDDGGFATQWRIGIAGGLAFEPATERPGV
IEAISGGVSQTWRIINGSLSGLGHMISGAISTCNLSGPIGIAQTSGAMASQGAESFIWFIAVLSTAVGLLNLFPVPALDG
GHLVFYAYEAVTGKPPSDKALRVLMTFGLATVLTLMLFALGNDIFCP

Sequences:

>Translated_447_residues
MTLIPQFGGLIWTIAAFILALSVIVAIHEYGHYIVGRWSGIHADVFSIGFGPVLFARTDKRGTRWQIAALPFGGYVKFAG
DADAASGKDDAAMAEVQNDPVRLRATMHGAPLWARTATVAAGPIFNFVLSIIVFAAVLLTSGIARDPLTVGEMRALPVEA
VGLQSGDEILGINGVDIPSTEDRDAYRAFIEALPFEPVLTYDVLRDGRTVTVDGPYMLPPLVNSLTPQSAAIRAGMAQGD
VIIAINGIPIYAFDELKNAVEGGNGATLDLTVWRAGETLEVSLTPKRVDEPQDDGGFATQWRIGIAGGLAFEPATERPGV
IEAISGGVSQTWRIINGSLSGLGHMISGAISTCNLSGPIGIAQTSGAMASQGAESFIWFIAVLSTAVGLLNLFPVPALDG
GHLVFYAYEAVTGKPPSDKALRVLMTFGLATVLTLMLFALGNDIFCP
>Mature_446_residues
TLIPQFGGLIWTIAAFILALSVIVAIHEYGHYIVGRWSGIHADVFSIGFGPVLFARTDKRGTRWQIAALPFGGYVKFAGD
ADAASGKDDAAMAEVQNDPVRLRATMHGAPLWARTATVAAGPIFNFVLSIIVFAAVLLTSGIARDPLTVGEMRALPVEAV
GLQSGDEILGINGVDIPSTEDRDAYRAFIEALPFEPVLTYDVLRDGRTVTVDGPYMLPPLVNSLTPQSAAIRAGMAQGDV
IIAINGIPIYAFDELKNAVEGGNGATLDLTVWRAGETLEVSLTPKRVDEPQDDGGFATQWRIGIAGGLAFEPATERPGVI
EAISGGVSQTWRIINGSLSGLGHMISGAISTCNLSGPIGIAQTSGAMASQGAESFIWFIAVLSTAVGLLNLFPVPALDGG
HLVFYAYEAVTGKPPSDKALRVLMTFGLATVLTLMLFALGNDIFCP

Specific function: Unknown

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain [H]

Homologues:

Organism=Escherichia coli, GI1786373, Length=441, Percent_Identity=31.2925170068027, Blast_Score=196, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR004387
- InterPro:   IPR008915 [H]

Pfam domain/function: PF00595 PDZ; PF02163 Peptidase_M50 [H]

EC number: NA

Molecular weight: Translated: 47099; Mature: 46968

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLIPQFGGLIWTIAAFILALSVIVAIHEYGHYIVGRWSGIHADVFSIGFGPVLFARTDK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHCCCCCEEEEEECC
RGTRWQIAALPFGGYVKFAGDADAASGKDDAAMAEVQNDPVRLRATMHGAPLWARTATVA
CCCEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHCCCCEEEEEEECCCCCHHHHHHHH
AGPIFNFVLSIIVFAAVLLTSGIARDPLTVGEMRALPVEAVGLQSGDEILGINGVDIPST
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCEECCCCCCCEEECCCCCCCCC
EDRDAYRAFIEALPFEPVLTYDVLRDGRTVTVDGPYMLPPLVNSLTPQSAAIRAGMAQGD
CCHHHHHHHHHHCCCCCEEEHHHHCCCCEEEECCCEECCHHHHHCCCCHHHHHCCCCCCC
VIIAINGIPIYAFDELKNAVEGGNGATLDLTVWRAGETLEVSLTPKRVDEPQDDGGFATQ
EEEEECCEEEEEHHHHHHHHCCCCCCEEEEEEEECCCEEEEEECHHHCCCCCCCCCEEEE
WRIGIAGGLAFEPATERPGVIEAISGGVSQTWRIINGSLSGLGHMISGAISTCNLSGPIG
EEEEEECCEEECCCCCCCCHHHHHCCCHHHHHHEECCCHHHHHHHHHHHHHCCCCCCCCC
IAQTSGAMASQGAESFIWFIAVLSTAVGLLNLFPVPALDGGHLVFYAYEAVTGKPPSDKA
EEECCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCHHH
LRVLMTFGLATVLTLMLFALGNDIFCP
HHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TLIPQFGGLIWTIAAFILALSVIVAIHEYGHYIVGRWSGIHADVFSIGFGPVLFARTDK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHCCCCCEEEEEECC
RGTRWQIAALPFGGYVKFAGDADAASGKDDAAMAEVQNDPVRLRATMHGAPLWARTATVA
CCCEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHCCCCEEEEEEECCCCCHHHHHHHH
AGPIFNFVLSIIVFAAVLLTSGIARDPLTVGEMRALPVEAVGLQSGDEILGINGVDIPST
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCEECCCCCCCEEECCCCCCCCC
EDRDAYRAFIEALPFEPVLTYDVLRDGRTVTVDGPYMLPPLVNSLTPQSAAIRAGMAQGD
CCHHHHHHHHHHCCCCCEEEHHHHCCCCEEEECCCEECCHHHHHCCCCHHHHHCCCCCCC
VIIAINGIPIYAFDELKNAVEGGNGATLDLTVWRAGETLEVSLTPKRVDEPQDDGGFATQ
EEEEECCEEEEEHHHHHHHHCCCCCCEEEEEEEECCCEEEEEECHHHCCCCCCCCCEEEE
WRIGIAGGLAFEPATERPGVIEAISGGVSQTWRIINGSLSGLGHMISGAISTCNLSGPIG
EEEEEECCEEECCCCCCCCHHHHHCCCHHHHHHEECCCHHHHHHHHHHHHHCCCCCCCCC
IAQTSGAMASQGAESFIWFIAVLSTAVGLLNLFPVPALDGGHLVFYAYEAVTGKPPSDKA
EEECCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCHHH
LRVLMTFGLATVLTLMLFALGNDIFCP
HHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]