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The map label for this gene is glnA [H]

Identifier: 110679805

GI number: 110679805

Start: 2457282

End: 2458685

Strand: Reverse

Name: glnA [H]

Synonym: RD1_2569

Alternate gene names: 110679805

Gene position: 2458685-2457282 (Counterclockwise)

Preceding gene: 110679806

Following gene: 110679802

Centisome position: 59.49

GC content: 55.48

Gene sequence:

>1404_bases
ATGAGCAAAGAACTACTGAAGATGATTGCGGACGAAGGTGTTGAATACGTCGATATCCGCTTTACCGACCCCCGCGGCAA
GCTGCAGCACGTCACGGTCATCGCTGATGAGGTGGACGAAGACTTCCTCGAAGAAGGCTTCATGTTTGACGGTTCCTCGA
TTGCCGGTTGGAAGTCGATCGAAAACTCCGACATGAAACTGATGCCGGATGTGGAAAGCGCCTATGTCGACCCGTTCTAT
GCAGAAAAAACCGTGTGCCTGCATTGCTCGATCGTGGAGCCGGATACCGGCGAACCCTATGAGCGTGACCCACGCTCGAC
AGCGCAGAAAGCCGAAGCGTTTCTGAAATCATCGGGCATTGGTGATGTGTCCTACTGGGGCCCCGAGGCGGAATTCTTCC
TGTTTGACAACGTCAAATACTCCAATTCGATCAACAAGGTATCTTACGAAGTTGATGCGGTGGATGCATCGTGGAACACA
GATACGGATTATGAGATGGGCAACATGGGCCACCGCCCCGGCGTCAAGGGTGGCTATTTCCCGGTCAACCCGATTGACGA
ATCCCAGGACCTGCGTTCCGAAATGTTGTCGACGATGAAACGTCTGGGCATGAAAGTGGACAAGCACCATCACGAGGTTG
CGTCCTGTCAGCACGAGTTGGGCCTGATCTTTGGCAGCCTGACCAAACAGGCGGATGAGTTGCAGAAATACAAATATGTG
ATCCACAACGTGGCCCACGCCTATGGCAAATCCGCAACCTTCATGCCCAAGCCGATCTATGGCGATAACGGCACGGGCAT
GCACTGCAACATGTCGATCTGGAAAGATGGCAAGCCGCTCTTTGCGGGCGACAAATACGCGGACCTGAGCCAGGAAGCGC
TGTATTACATCGGCGGCATCCTAAAGCACGCCAAGGCGCTGAACGCCTTCACCAACCCGGCGACGAACAGCTACAAACGC
CTGATCCCGGGGTTTGAAGCGCCGGTACTGCGTGCCTATTCCGCCCGCAACCGGTCAGGCTGCGTGCGTATTCCATGGAC
CGAAAACCCGAAGGCCAAGCGCGTCGAGGCACGCTTCCCGGATCCGGCGGCGAACCCGTACCTGTGCTTTGCGGCACTGC
TGATGGCAGGGCTGGACGGTATCATGAACAAAATCGACCCCGGCGAGGCCATGGACAAAAACCTCTATGATCTGCCTGCT
GAGGAGTTGGCCGGGATCCCCACAGTCTGCGGATCGCTGCGCGAAGCAATTGAATCGCTTCAGGCGGACATGGACTTCCT
GCTGGCGGGTGACGTCTTTACCAAGGATCAGATCGAAGGATACATCGAGCTGAAAATGGAAGAGATCGAGACATACGAAC
ACACGCCGCACCCGGTCGAATTTGGCATGTATTACAGCTGCTAA

Upstream 100 bases:

>100_bases
TGAACAAACCATCCGCATCCGCACAGGCGAGACCGGGTCAGACGCGCTCTGAACCCAAGCGGCCCCACACGACCATTCCA
GAAAAAAGGGATATCCGAGA

Downstream 100 bases:

>100_bases
GTCACTATCGGGCTGTACCGTTGCAAATCAAAGGCGCCTCACGGGGCGCCTTTTTTGCATCCCTGGCCATCCCTTGAGAT
TGCGTTGCGCTGAACTCTCA

Product: glutamine synthetase, type I

Products: NA

Alternate protein names: GS; Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSKELLKMIADEGVEYVDIRFTDPRGKLQHVTVIADEVDEDFLEEGFMFDGSSIAGWKSIENSDMKLMPDVESAYVDPFY
AEKTVCLHCSIVEPDTGEPYERDPRSTAQKAEAFLKSSGIGDVSYWGPEAEFFLFDNVKYSNSINKVSYEVDAVDASWNT
DTDYEMGNMGHRPGVKGGYFPVNPIDESQDLRSEMLSTMKRLGMKVDKHHHEVASCQHELGLIFGSLTKQADELQKYKYV
IHNVAHAYGKSATFMPKPIYGDNGTGMHCNMSIWKDGKPLFAGDKYADLSQEALYYIGGILKHAKALNAFTNPATNSYKR
LIPGFEAPVLRAYSARNRSGCVRIPWTENPKAKRVEARFPDPAANPYLCFAALLMAGLDGIMNKIDPGEAMDKNLYDLPA
EELAGIPTVCGSLREAIESLQADMDFLLAGDVFTKDQIEGYIELKMEEIETYEHTPHPVEFGMYYSC

Sequences:

>Translated_467_residues
MSKELLKMIADEGVEYVDIRFTDPRGKLQHVTVIADEVDEDFLEEGFMFDGSSIAGWKSIENSDMKLMPDVESAYVDPFY
AEKTVCLHCSIVEPDTGEPYERDPRSTAQKAEAFLKSSGIGDVSYWGPEAEFFLFDNVKYSNSINKVSYEVDAVDASWNT
DTDYEMGNMGHRPGVKGGYFPVNPIDESQDLRSEMLSTMKRLGMKVDKHHHEVASCQHELGLIFGSLTKQADELQKYKYV
IHNVAHAYGKSATFMPKPIYGDNGTGMHCNMSIWKDGKPLFAGDKYADLSQEALYYIGGILKHAKALNAFTNPATNSYKR
LIPGFEAPVLRAYSARNRSGCVRIPWTENPKAKRVEARFPDPAANPYLCFAALLMAGLDGIMNKIDPGEAMDKNLYDLPA
EELAGIPTVCGSLREAIESLQADMDFLLAGDVFTKDQIEGYIELKMEEIETYEHTPHPVEFGMYYSC
>Mature_466_residues
SKELLKMIADEGVEYVDIRFTDPRGKLQHVTVIADEVDEDFLEEGFMFDGSSIAGWKSIENSDMKLMPDVESAYVDPFYA
EKTVCLHCSIVEPDTGEPYERDPRSTAQKAEAFLKSSGIGDVSYWGPEAEFFLFDNVKYSNSINKVSYEVDAVDASWNTD
TDYEMGNMGHRPGVKGGYFPVNPIDESQDLRSEMLSTMKRLGMKVDKHHHEVASCQHELGLIFGSLTKQADELQKYKYVI
HNVAHAYGKSATFMPKPIYGDNGTGMHCNMSIWKDGKPLFAGDKYADLSQEALYYIGGILKHAKALNAFTNPATNSYKRL
IPGFEAPVLRAYSARNRSGCVRIPWTENPKAKRVEARFPDPAANPYLCFAALLMAGLDGIMNKIDPGEAMDKNLYDLPAE
ELAGIPTVCGSLREAIESLQADMDFLLAGDVFTKDQIEGYIELKMEEIETYEHTPHPVEFGMYYSC

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=469, Percent_Identity=60.5543710021322, Blast_Score=616, Evalue=1e-177,
Organism=Escherichia coli, GI87081870, Length=397, Percent_Identity=23.4256926952141, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52276; Mature: 52145

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKELLKMIADEGVEYVDIRFTDPRGKLQHVTVIADEVDEDFLEEGFMFDGSSIAGWKSI
CCHHHHHHHHHCCCEEEEEEEECCCCCEEEEEEEHHHHHHHHHHCCCEECCCCCCCCCCC
ENSDMKLMPDVESAYVDPFYAEKTVCLHCSIVEPDTGEPYERDPRSTAQKAEAFLKSSGI
CCCCCEECCCHHHHHCCCHHCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCC
GDVSYWGPEAEFFLFDNVKYSNSINKVSYEVDAVDASWNTDTDYEMGNMGHRPGVKGGYF
CCCCCCCCCCEEEEEECCEECCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
PVNPIDESQDLRSEMLSTMKRLGMKVDKHHHEVASCQHELGLIFGSLTKQADELQKYKYV
CCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHNVAHAYGKSATFMPKPIYGDNGTGMHCNMSIWKDGKPLFAGDKYADLSQEALYYIGGI
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHCHHHHHHHHHHHH
LKHAKALNAFTNPATNSYKRLIPGFEAPVLRAYSARNRSGCVRIPWTENPKAKRVEARFP
HHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHCCCCCCEEECCCCCCCCCCEEECCCC
DPAANPYLCFAALLMAGLDGIMNKIDPGEAMDKNLYDLPAEELAGIPTVCGSLREAIESL
CCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHH
QADMDFLLAGDVFTKDQIEGYIELKMEEIETYEHTPHPVEFGMYYSC
HHCCCEEEECCCCCHHHHCEEEEEEHHHHHHHCCCCCCEECCCEECC
>Mature Secondary Structure 
SKELLKMIADEGVEYVDIRFTDPRGKLQHVTVIADEVDEDFLEEGFMFDGSSIAGWKSI
CHHHHHHHHHCCCEEEEEEEECCCCCEEEEEEEHHHHHHHHHHCCCEECCCCCCCCCCC
ENSDMKLMPDVESAYVDPFYAEKTVCLHCSIVEPDTGEPYERDPRSTAQKAEAFLKSSGI
CCCCCEECCCHHHHHCCCHHCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCC
GDVSYWGPEAEFFLFDNVKYSNSINKVSYEVDAVDASWNTDTDYEMGNMGHRPGVKGGYF
CCCCCCCCCCEEEEEECCEECCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
PVNPIDESQDLRSEMLSTMKRLGMKVDKHHHEVASCQHELGLIFGSLTKQADELQKYKYV
CCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHNVAHAYGKSATFMPKPIYGDNGTGMHCNMSIWKDGKPLFAGDKYADLSQEALYYIGGI
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHCHHHHHHHHHHHH
LKHAKALNAFTNPATNSYKRLIPGFEAPVLRAYSARNRSGCVRIPWTENPKAKRVEARFP
HHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHCCCCCCEEECCCCCCCCCCEEECCCC
DPAANPYLCFAALLMAGLDGIMNKIDPGEAMDKNLYDLPAEELAGIPTVCGSLREAIESL
CCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHH
QADMDFLLAGDVFTKDQIEGYIELKMEEIETYEHTPHPVEFGMYYSC
HHCCCEEEECCCCCHHHHCEEEEEEHHHHHHHCCCCCCEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7921264 [H]