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The map label for this gene is metG

Identifier: 110679753

GI number: 110679753

Start: 2402719

End: 2404437

Strand: Reverse

Name: metG

Synonym: RD1_2516

Alternate gene names: 110679753

Gene position: 2404437-2402719 (Counterclockwise)

Preceding gene: 110679754

Following gene: 110679751

Centisome position: 58.18

GC content: 55.21

Gene sequence:

>1719_bases
TTGGAACGTCACCTCATCACTTCTGCGATTCCTTATATCAATGGTATCAAGCACTTGGGAAATCTGGTCGGCAGCCAATT
GCCCGCCGACCTCTATGCCAGATATCTGCGCGCGCGCGGCCATGAGGTTCTGTTTTTATGTGCAACGGACGAACATGGCA
CGCCCGCAGAACTGGCCGCAGCCAAAGCCGGAAAACCGGTTGATGAGTATTGCGCAGAAATGCACGCGATCCAGTCGGAG
ATCGCCAAGGGTTTCCGGTTATCCTTTGATCATTTCGGACGCTCCTCAAGTGCGCAAAACCATCGTCTGACGCAGCATTT
CGCAGGCAAACTGGACGAAGCGAACCTGATCGACGAAGTGATTGAAAAGCAGGTCTATTCACATGCGGATGGCCGCTTTC
TGCCAGATCGCTACATCGAAGGGACCTGCCCCAATTGCGCCTATGACAAGGCGCGCGGAGATCAATGCGAGAACTGCACC
AAGCAACTGGACCCGACCGATCTGATTGAACCGCGTTCCGCGATTTCAGGTTCGACCGACCTTGAGGTGCGCGAGACCAA
GCATCTCTACCTCAAACAATCCGTGCTCAAGGACCAGTTGGACGCCTGGATTGACAGCAAAACCGACTGGCCCGTCCTGA
CGACCTCGATCGCCAAGAAATGGCTGCATGATGGGGACGGTCTGCGGGATCGCGGTATTACGAGGGACCTCGATTGGGGC
ATCCCCGTCAAGAAAGGCACGCAGGATTGGCCGGGCATGGAGGGCAAAGTGTTCTATGTCTGGTTCGATGCGCCGATTGA
ATATATCGCATGTGCGTCAGAGTGGGCCGAGGCAAACAATCTGGAACAATCCGACTGGGAAAGATGGTGGCGGACGGACA
AGGGTGCAGATGATGTGCGCTACACGCAATTCATGGGCAAGGACAACGTGCCGTTCCACACCTTGAGTTTCCCCGCTACC
CTGCTCGGCTCGGGTGAGCCGTGGAAAATGGTCGATCACCTCAAGTCCTTCAATTACCTGAATTATGATGGCGGACAGTT
TTCGACATCGCAGGGTCGGGGTATTTTCATGGATCAGGCGCTGGAGTTGTTGCCGGCGGATTACTGGCGTTGGTGGTTGC
TCAGCCATGCGCCCGAAAACAGCGATTCCGAGTTTACCTGGGAGAACTTCCAGTCATCAGTAAACAAGGATCTGGCAGAC
GTCTTGGGCAATTTTGTCAGCCGGATTACAAAGTTCTGCGCTGCGAAATTCGGCAGCGAAGTGCCGGCCGGCGGAGCATA
TGGCTCGGCGGAGCTGGAATTGCTGACCAAGCTGGATGAGAGCCTCACGCGCTATCAGGATTACATGGGCGCGATGGAAG
TTCGAAAATCGGCACAGGAGTTGCGCGCGATCTGGGCCAGTGGAAACGAGTATCTGCAAACCGTTGCCCCGTGGTCTGTT
TTCAAGACAGACCCCGAAACCGCCGCCATGCAAACGCGGCTAGGGCTGAATTTGGCCCTCCTGTTCGGCGTCTTGTCTTC
ACCTTTTGTGCCGGATGCCTCTGAAAAGATCCTGAAATGCCTCGGACATCCCTCTGCGCGTTGGCCAGATACGTGCACAG
ACTTCGTAACAAGCATGAAACCGGGTGATCCGTTCGAAGTGCCCGAAGTGCTTTTTGCAAAGCTGACGGATGAGGATCGC
GCAACCTATAGTGCGAAGTTTTCGGGCACCCAAAGCTGA

Upstream 100 bases:

>100_bases
AAAAAGCCGCAAGCGTGAAACGAAGCGTTAATTTCGCCGTGGCCCTCACGGATCACCTGAGGTAGACCCTGTGCAAGAAA
TCAGCCTACGGAGCCACGTT

Downstream 100 bases:

>100_bases
AGACTTCAGATGTCCTGATGCCGAAAGTGCAGCGCGGCGTAAGTTGATTGCCTATCAAAGGCGCCACCTTCATGCATGCA
AACTCGCTCTGCGGGGCAGT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MERHLITSAIPYINGIKHLGNLVGSQLPADLYARYLRARGHEVLFLCATDEHGTPAELAAAKAGKPVDEYCAEMHAIQSE
IAKGFRLSFDHFGRSSSAQNHRLTQHFAGKLDEANLIDEVIEKQVYSHADGRFLPDRYIEGTCPNCAYDKARGDQCENCT
KQLDPTDLIEPRSAISGSTDLEVRETKHLYLKQSVLKDQLDAWIDSKTDWPVLTTSIAKKWLHDGDGLRDRGITRDLDWG
IPVKKGTQDWPGMEGKVFYVWFDAPIEYIACASEWAEANNLEQSDWERWWRTDKGADDVRYTQFMGKDNVPFHTLSFPAT
LLGSGEPWKMVDHLKSFNYLNYDGGQFSTSQGRGIFMDQALELLPADYWRWWLLSHAPENSDSEFTWENFQSSVNKDLAD
VLGNFVSRITKFCAAKFGSEVPAGGAYGSAELELLTKLDESLTRYQDYMGAMEVRKSAQELRAIWASGNEYLQTVAPWSV
FKTDPETAAMQTRLGLNLALLFGVLSSPFVPDASEKILKCLGHPSARWPDTCTDFVTSMKPGDPFEVPEVLFAKLTDEDR
ATYSAKFSGTQS

Sequences:

>Translated_572_residues
MERHLITSAIPYINGIKHLGNLVGSQLPADLYARYLRARGHEVLFLCATDEHGTPAELAAAKAGKPVDEYCAEMHAIQSE
IAKGFRLSFDHFGRSSSAQNHRLTQHFAGKLDEANLIDEVIEKQVYSHADGRFLPDRYIEGTCPNCAYDKARGDQCENCT
KQLDPTDLIEPRSAISGSTDLEVRETKHLYLKQSVLKDQLDAWIDSKTDWPVLTTSIAKKWLHDGDGLRDRGITRDLDWG
IPVKKGTQDWPGMEGKVFYVWFDAPIEYIACASEWAEANNLEQSDWERWWRTDKGADDVRYTQFMGKDNVPFHTLSFPAT
LLGSGEPWKMVDHLKSFNYLNYDGGQFSTSQGRGIFMDQALELLPADYWRWWLLSHAPENSDSEFTWENFQSSVNKDLAD
VLGNFVSRITKFCAAKFGSEVPAGGAYGSAELELLTKLDESLTRYQDYMGAMEVRKSAQELRAIWASGNEYLQTVAPWSV
FKTDPETAAMQTRLGLNLALLFGVLSSPFVPDASEKILKCLGHPSARWPDTCTDFVTSMKPGDPFEVPEVLFAKLTDEDR
ATYSAKFSGTQS
>Mature_572_residues
MERHLITSAIPYINGIKHLGNLVGSQLPADLYARYLRARGHEVLFLCATDEHGTPAELAAAKAGKPVDEYCAEMHAIQSE
IAKGFRLSFDHFGRSSSAQNHRLTQHFAGKLDEANLIDEVIEKQVYSHADGRFLPDRYIEGTCPNCAYDKARGDQCENCT
KQLDPTDLIEPRSAISGSTDLEVRETKHLYLKQSVLKDQLDAWIDSKTDWPVLTTSIAKKWLHDGDGLRDRGITRDLDWG
IPVKKGTQDWPGMEGKVFYVWFDAPIEYIACASEWAEANNLEQSDWERWWRTDKGADDVRYTQFMGKDNVPFHTLSFPAT
LLGSGEPWKMVDHLKSFNYLNYDGGQFSTSQGRGIFMDQALELLPADYWRWWLLSHAPENSDSEFTWENFQSSVNKDLAD
VLGNFVSRITKFCAAKFGSEVPAGGAYGSAELELLTKLDESLTRYQDYMGAMEVRKSAQELRAIWASGNEYLQTVAPWSV
FKTDPETAAMQTRLGLNLALLFGVLSSPFVPDASEKILKCLGHPSARWPDTCTDFVTSMKPGDPFEVPEVLFAKLTDEDR
ATYSAKFSGTQS

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily

Homologues:

Organism=Homo sapiens, GI14043022, Length=575, Percent_Identity=36.3478260869565, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI24308436, Length=547, Percent_Identity=25.7769652650823, Blast_Score=139, Evalue=7e-33,
Organism=Escherichia coli, GI1788432, Length=522, Percent_Identity=30.4597701149425, Blast_Score=241, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI17540794, Length=576, Percent_Identity=35.0694444444444, Blast_Score=347, Evalue=7e-96,
Organism=Caenorhabditis elegans, GI212645096, Length=538, Percent_Identity=23.7918215613383, Blast_Score=108, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71992781, Length=365, Percent_Identity=24.1095890410959, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6321704, Length=573, Percent_Identity=36.3001745200698, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24655311, Length=583, Percent_Identity=37.3927958833619, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI21358483, Length=563, Percent_Identity=24.866785079929, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_ROSDO (Q166L9)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_682760.1
- STRING:   Q166L9
- GeneID:   4195264
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_2516
- NMPDR:   fig|375451.6.peg.2337
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   WSANAIS
- PhylomeDB:   Q166L9
- ProtClustDB:   PRK12268
- BioCyc:   RDEN375451:RD1_2516-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398

Pfam domain/function: PF09334 tRNA-synt_1g; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 64346; Mature: 64346

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 349-349

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERHLITSAIPYINGIKHLGNLVGSQLPADLYARYLRARGHEVLFLCATDEHGTPAELAA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHH
AKAGKPVDEYCAEMHAIQSEIAKGFRLSFDHFGRSSSAQNHRLTQHFAGKLDEANLIDEV
HHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHH
IEKQVYSHADGRFLPDRYIEGTCPNCAYDKARGDQCENCTKQLDPTDLIEPRSAISGSTD
HHHHHHHCCCCCCCCCHHCCCCCCCCCCHHCCCCHHHHHHHHCCCHHHCCCHHHCCCCCC
LEVRETKHLYLKQSVLKDQLDAWIDSKTDWPVLTTSIAKKWLHDGDGLRDRGITRDLDWG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
IPVKKGTQDWPGMEGKVFYVWFDAPIEYIACASEWAEANNLEQSDWERWWRTDKGADDVR
CCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHH
YTQFMGKDNVPFHTLSFPATLLGSGEPWKMVDHLKSFNYLNYDGGQFSTSQGRGIFMDQA
HHHHHCCCCCCEEEECCCHHHHCCCCCHHHHHHHHHCCCCCCCCCCEECCCCCCCHHHHH
LELLPADYWRWWLLSHAPENSDSEFTWENFQSSVNKDLADVLGNFVSRITKFCAAKFGSE
HHHHCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VPAGGAYGSAELELLTKLDESLTRYQDYMGAMEVRKSAQELRAIWASGNEYLQTVAPWSV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHH
FKTDPETAAMQTRLGLNLALLFGVLSSPFVPDASEKILKCLGHPSARWPDTCTDFVTSMK
CCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHCC
PGDPFEVPEVLFAKLTDEDRATYSAKFSGTQS
CCCCCCHHHHHHHHHCCCHHHEEEEECCCCCC
>Mature Secondary Structure
MERHLITSAIPYINGIKHLGNLVGSQLPADLYARYLRARGHEVLFLCATDEHGTPAELAA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHH
AKAGKPVDEYCAEMHAIQSEIAKGFRLSFDHFGRSSSAQNHRLTQHFAGKLDEANLIDEV
HHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHH
IEKQVYSHADGRFLPDRYIEGTCPNCAYDKARGDQCENCTKQLDPTDLIEPRSAISGSTD
HHHHHHHCCCCCCCCCHHCCCCCCCCCCHHCCCCHHHHHHHHCCCHHHCCCHHHCCCCCC
LEVRETKHLYLKQSVLKDQLDAWIDSKTDWPVLTTSIAKKWLHDGDGLRDRGITRDLDWG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
IPVKKGTQDWPGMEGKVFYVWFDAPIEYIACASEWAEANNLEQSDWERWWRTDKGADDVR
CCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHH
YTQFMGKDNVPFHTLSFPATLLGSGEPWKMVDHLKSFNYLNYDGGQFSTSQGRGIFMDQA
HHHHHCCCCCCEEEECCCHHHHCCCCCHHHHHHHHHCCCCCCCCCCEECCCCCCCHHHHH
LELLPADYWRWWLLSHAPENSDSEFTWENFQSSVNKDLADVLGNFVSRITKFCAAKFGSE
HHHHCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VPAGGAYGSAELELLTKLDESLTRYQDYMGAMEVRKSAQELRAIWASGNEYLQTVAPWSV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHH
FKTDPETAAMQTRLGLNLALLFGVLSSPFVPDASEKILKCLGHPSARWPDTCTDFVTSMK
CCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHCC
PGDPFEVPEVLFAKLTDEDRATYSAKFSGTQS
CCCCCCHHHHHHHHHCCCHHHEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA