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The map label for this gene is narH [H]

Identifier: 110679688

GI number: 110679688

Start: 2329650

End: 2331170

Strand: Direct

Name: narH [H]

Synonym: RD1_2443

Alternate gene names: 110679688

Gene position: 2329650-2331170 (Clockwise)

Preceding gene: 110679687

Following gene: 110679689

Centisome position: 56.37

GC content: 58.38

Gene sequence:

>1521_bases
ATGAGAGTTCGTGCGCAAATCGGTATGGTGTTGAACCTCGATAAATGTATCGGGTGCCACACCTGCTCCGTGACCTGCAA
GAACGTGTGGACCACACGGGACGGGGTCGAATACGCCTGGTTCAACAACGTCGAGACAAAGCCGGGCACCGGCTATCCGA
CGGATTGGGAAAATCAGGCCAAGTGGAACGGCGGTTGGGAACGGACCAAGTCCGGCAAACTGCAGCCCAAACAGGGCAGC
AAATGGCGGATCCTGTCCAACATCTTTGCCAACCCCGATCTGCCCGAGATCGACGACTATTATGAGCCGTTCGATTTCGA
CCACGATCACCTGAAGTCAGCCCCGGAAATGGAAGCGTTCCCCACGGCGCGCCCCCGCTCCAAGATCACCGGTGAACGGA
TCGAGAAGATCGAGAAAGGCCCCAACTGGGAGGAAATCCTCGGTGGTGAGTTCTCGAAACGCTCGGAAGACTACAACTTC
GAAGGCATCCAGAAGGAGATCTACGGGGAATACGAGAACACCTTCATGATGTATCTCCCCCGGCTCTGTGAGCACTGCCT
CAATCCGGCCTGTGCGGCCTCCTGCCCATCGGGCGCGATCTACAAACGCGAGGAAGACGGCATTGTCCTGATCGATCAGG
AGAAGTGCCGCGGCTGGCGGATGTGCGTCTCGGGCTGCCCCTACAAGAAGGTGTATTACAACTGGGAAAGCGGAAAATCT
GAGAAATGCACCCTGTGCTACCCCCGGATTGAAAGCGGCAACCCGACCGTGTGTTCGGAAACCTGCGTGGGTCGCATCCG
GTATCTGGGCGTGATGCTCTATGATGCGGACAAGATCGACACCGCCGCGAATGCGCCGACGGATCAAGAGCTTTATGATG
CGCAGCTGGATGTGTTCCTCGACCCCAACGATCCTGTGGTCATTGAGACCGCAAGGGCCGACGGTATTCCGGAGGACTGG
ATCAAGGCGGCTCAGGAAAGCCCCATCTGGAAAATGGCCATGGAATGGAAAGTCGCCTTCCCGCTGCATCCGGAATACCG
GACGCTGCCGATGGTGTGGTACATCCCGCCACTCTCACCGATCCAGAACGCCGCCGAGGCCGGGAAGATCGGCACGGATG
GCATGATGCCCAACGTCAAGGATTTGCGGATTCCCGTTCGGTATCTCGCGAACATGCTCACGGCAGGTGACGAGGCCCCG
GTGGTGTCGGCGCTCGAACGGATGATGGCCATGCGCGCCTACATGCGGTCGAAAACCGTGGACGGTGTGATTGACGAGGC
CATCGCCGCACGGGTCGGCCTGAGCGGGCGTGTGATCGAGGACATGTACAAGATCATGGCCCTTGCCGATTACGAGGACC
GTTTCGTCATCCCGACAACCCACCGTGAGCAGGTCGAAGAAGCCTATGACCTCAAGGGGGGCTGTGGATTTACCGACGGC
AACGGTTGTTCGACCGGGACGTCCAAGCCCTCGCTTTTTGGCAAAGGGAAGAAACCTCTTAAAATGCCGGAGGCAATGTG
A

Upstream 100 bases:

>100_bases
ACGGCACCGTGGGCTGCAACCGGGACGAGTTCGTCATCGTGCGGAAAATGCAAAAGGTCGACTGGCTCGACAACGAAGCT
ACCCAGAAGGAGGCAGCGGA

Downstream 100 bases:

>100_bases
TGACCATTGATCGTACATTCAAAGCCATTTCGCTGGTCCTGAGTTACCCGACGCGCGAGCTGCAGCATGCCATGCCCGAG
ATCGGAGGCGTTCTGGCCTC

Product: nitrate reductase, beta subunit

Products: NA

Alternate protein names: Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MRVRAQIGMVLNLDKCIGCHTCSVTCKNVWTTRDGVEYAWFNNVETKPGTGYPTDWENQAKWNGGWERTKSGKLQPKQGS
KWRILSNIFANPDLPEIDDYYEPFDFDHDHLKSAPEMEAFPTARPRSKITGERIEKIEKGPNWEEILGGEFSKRSEDYNF
EGIQKEIYGEYENTFMMYLPRLCEHCLNPACAASCPSGAIYKREEDGIVLIDQEKCRGWRMCVSGCPYKKVYYNWESGKS
EKCTLCYPRIESGNPTVCSETCVGRIRYLGVMLYDADKIDTAANAPTDQELYDAQLDVFLDPNDPVVIETARADGIPEDW
IKAAQESPIWKMAMEWKVAFPLHPEYRTLPMVWYIPPLSPIQNAAEAGKIGTDGMMPNVKDLRIPVRYLANMLTAGDEAP
VVSALERMMAMRAYMRSKTVDGVIDEAIAARVGLSGRVIEDMYKIMALADYEDRFVIPTTHREQVEEAYDLKGGCGFTDG
NGCSTGTSKPSLFGKGKKPLKMPEAM

Sequences:

>Translated_506_residues
MRVRAQIGMVLNLDKCIGCHTCSVTCKNVWTTRDGVEYAWFNNVETKPGTGYPTDWENQAKWNGGWERTKSGKLQPKQGS
KWRILSNIFANPDLPEIDDYYEPFDFDHDHLKSAPEMEAFPTARPRSKITGERIEKIEKGPNWEEILGGEFSKRSEDYNF
EGIQKEIYGEYENTFMMYLPRLCEHCLNPACAASCPSGAIYKREEDGIVLIDQEKCRGWRMCVSGCPYKKVYYNWESGKS
EKCTLCYPRIESGNPTVCSETCVGRIRYLGVMLYDADKIDTAANAPTDQELYDAQLDVFLDPNDPVVIETARADGIPEDW
IKAAQESPIWKMAMEWKVAFPLHPEYRTLPMVWYIPPLSPIQNAAEAGKIGTDGMMPNVKDLRIPVRYLANMLTAGDEAP
VVSALERMMAMRAYMRSKTVDGVIDEAIAARVGLSGRVIEDMYKIMALADYEDRFVIPTTHREQVEEAYDLKGGCGFTDG
NGCSTGTSKPSLFGKGKKPLKMPEAM
>Mature_506_residues
MRVRAQIGMVLNLDKCIGCHTCSVTCKNVWTTRDGVEYAWFNNVETKPGTGYPTDWENQAKWNGGWERTKSGKLQPKQGS
KWRILSNIFANPDLPEIDDYYEPFDFDHDHLKSAPEMEAFPTARPRSKITGERIEKIEKGPNWEEILGGEFSKRSEDYNF
EGIQKEIYGEYENTFMMYLPRLCEHCLNPACAASCPSGAIYKREEDGIVLIDQEKCRGWRMCVSGCPYKKVYYNWESGKS
EKCTLCYPRIESGNPTVCSETCVGRIRYLGVMLYDADKIDTAANAPTDQELYDAQLDVFLDPNDPVVIETARADGIPEDW
IKAAQESPIWKMAMEWKVAFPLHPEYRTLPMVWYIPPLSPIQNAAEAGKIGTDGMMPNVKDLRIPVRYLANMLTAGDEAP
VVSALERMMAMRAYMRSKTVDGVIDEAIAARVGLSGRVIEDMYKIMALADYEDRFVIPTTHREQVEEAYDLKGGCGFTDG
NGCSTGTSKPSLFGKGKKPLKMPEAM

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons ar

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=494, Percent_Identity=68.6234817813765, Blast_Score=749, Evalue=0.0,
Organism=Escherichia coli, GI1787740, Length=498, Percent_Identity=66.6666666666667, Blast_Score=736, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=133, Percent_Identity=36.8421052631579, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1787122, Length=134, Percent_Identity=38.0597014925373, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=36.7816091954023, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790326, Length=87, Percent_Identity=35.632183908046, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789370, Length=107, Percent_Identity=35.5140186915888, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI2367345, Length=101, Percent_Identity=27.7227722772277, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 57151; Mature: 57151

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVRAQIGMVLNLDKCIGCHTCSVTCKNVWTTRDGVEYAWFNNVETKPGTGYPTDWENQA
CCCCEEECEEEEHHHHHCCCEEEEEECCEEECCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWNGGWERTKSGKLQPKQGSKWRILSNIFANPDLPEIDDYYEPFDFDHDHLKSAPEMEAF
CCCCCCCCCCCCCCCCCCCCCEEEHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCC
PTARPRSKITGERIEKIEKGPNWEEILGGEFSKRSEDYNFEGIQKEIYGEYENTFMMYLP
CCCCCCHHCCHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHH
RLCEHCLNPACAASCPSGAIYKREEDGIVLIDQEKCRGWRMCVSGCPYKKVYYNWESGKS
HHHHHHCCCHHHCCCCCCCCEEECCCCEEEEEHHHCCCHHHHHCCCCCCEEEEECCCCCC
EKCTLCYPRIESGNPTVCSETCVGRIRYLGVMLYDADKIDTAANAPTDQELYDAQLDVFL
CCEEEEECEECCCCCCEEHHHHHHHHHHHEEEEECCCCCCCCCCCCCCHHHHCCEEEEEE
DPNDPVVIETARADGIPEDWIKAAQESPIWKMAMEWKVAFPLHPEYRTLPMVWYIPPLSP
CCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEEEECCCCH
IQNAAEAGKIGTDGMMPNVKDLRIPVRYLANMLTAGDEAPVVSALERMMAMRAYMRSKTV
HHHHHHHCCCCCCCCCCCHHHHEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCH
DGVIDEAIAARVGLSGRVIEDMYKIMALADYEDRFVIPTTHREQVEEAYDLKGGCGFTDG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHCCCCCCCCCCC
NGCSTGTSKPSLFGKGKKPLKMPEAM
CCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRVRAQIGMVLNLDKCIGCHTCSVTCKNVWTTRDGVEYAWFNNVETKPGTGYPTDWENQA
CCCCEEECEEEEHHHHHCCCEEEEEECCEEECCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWNGGWERTKSGKLQPKQGSKWRILSNIFANPDLPEIDDYYEPFDFDHDHLKSAPEMEAF
CCCCCCCCCCCCCCCCCCCCCEEEHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCC
PTARPRSKITGERIEKIEKGPNWEEILGGEFSKRSEDYNFEGIQKEIYGEYENTFMMYLP
CCCCCCHHCCHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHH
RLCEHCLNPACAASCPSGAIYKREEDGIVLIDQEKCRGWRMCVSGCPYKKVYYNWESGKS
HHHHHHCCCHHHCCCCCCCCEEECCCCEEEEEHHHCCCHHHHHCCCCCCEEEEECCCCCC
EKCTLCYPRIESGNPTVCSETCVGRIRYLGVMLYDADKIDTAANAPTDQELYDAQLDVFL
CCEEEEECEECCCCCCEEHHHHHHHHHHHEEEEECCCCCCCCCCCCCCHHHHCCEEEEEE
DPNDPVVIETARADGIPEDWIKAAQESPIWKMAMEWKVAFPLHPEYRTLPMVWYIPPLSP
CCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEEEECCCCH
IQNAAEAGKIGTDGMMPNVKDLRIPVRYLANMLTAGDEAPVVSALERMMAMRAYMRSKTV
HHHHHHHCCCCCCCCCCCHHHHEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCH
DGVIDEAIAARVGLSGRVIEDMYKIMALADYEDRFVIPTTHREQVEEAYDLKGGCGFTDG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHCCCCCCCCCCC
NGCSTGTSKPSLFGKGKKPLKMPEAM
CCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2674654; 8905232; 9278503; 2832376; 3053688; 1321049; 8383531; 8388253; 8664273; 9516445; 12910261 [H]