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The map label for this gene is potB [H]

Identifier: 110679657

GI number: 110679657

Start: 2297481

End: 2298311

Strand: Reverse

Name: potB [H]

Synonym: RD1_2408

Alternate gene names: 110679657

Gene position: 2298311-2297481 (Counterclockwise)

Preceding gene: 110679658

Following gene: 110679656

Centisome position: 55.61

GC content: 59.57

Gene sequence:

>831_bases
ATGAGGGGCCGGGCCGAGCGTATCGGGCTTCTCGCGCTGCCCGGCCTGAGCTTTCTGGCAATCATTTATGCGCTGCCGCT
TTTCATGTTGCTGCTCAAATCGTTCCAGATCGACGGTCAGTTTTCCGTGGAGGAATACGTCACCTTTTTCAGTGATGAGT
ATAATCTGAACGTGCTGTGGCGCACCCTGCGCGTGGCCCTGCTGACCACGGGTCTGGCGCTGATCATCGCCTATCCCACC
GCGATCATCATGGCGCGGGTGAAAGGCTTCTGGCTGTCGGTGTTTCTGGTCGCGATGGTGCTGCCGATGTCTGTCGGCGT
TGTCGTCAAAGCCTTTGCATGGACGATCCTTTTTCGCACGCGCGGCGTGCTGAACGACACGTTGATGTCGCTGGGCATCA
CGGAGGCACCGATCCGTATGCTTTTCACCGAAACAGCCCTCGTAATTGGTGCTGCGAACGTATTCCTGCCCTTCATGGTG
TTGCCGATCTACGCGGTGCTGCGCCAGATGGACAGCCGCCTGCCGGAGGCTGCGGCATCGTTGGGGGCAACGCCGGTCTT
TCGCTTCATGAACGTCACCCTGCCGCTCAGCCTGCCCGGCGTGGTCGCAGGATCCGCCTTCACCTTCAGCCTCGCCGTTT
CGATGTATGTGATCCCTTCGCTCATCGTGGGGGAAAGGCAGCAAACCCTGTCCATGCTCACCGCGCGCTCGTTTCTCTAC
CTGCGCAATGAAGCACTTGGGGCCACGATATCTTCAATCCTGCTGGTTATCGCGATCTCGGTGGTTCTGGGGTCAAGCTG
GCTTGCGAACCGTCTGGGGGCGCGGCAATGA

Upstream 100 bases:

>100_bases
TGCTGTGCGAACTGCCGGGCATGGGCTGCGGACTGTCCGCCGGGGACAGCGTCACGTTTGGCTGGGCCGCCGCGGACACA
CTCGTCTACGAAGCGGCATC

Downstream 100 bases:

>100_bases
CCGGAACCGACCGCCTTGCCCTCAGCCTGAGCTGGATTGTCGCCTTGGGCTGCGTCTTTTTCCTGATGTTGCCGATTGTC
ATTACTCTTGCGGCGTCTTT

Product: spermidine/putrescine ABC transporter, permease protein, putative

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MRGRAERIGLLALPGLSFLAIIYALPLFMLLLKSFQIDGQFSVEEYVTFFSDEYNLNVLWRTLRVALLTTGLALIIAYPT
AIIMARVKGFWLSVFLVAMVLPMSVGVVVKAFAWTILFRTRGVLNDTLMSLGITEAPIRMLFTETALVIGAANVFLPFMV
LPIYAVLRQMDSRLPEAAASLGATPVFRFMNVTLPLSLPGVVAGSAFTFSLAVSMYVIPSLIVGERQQTLSMLTARSFLY
LRNEALGATISSILLVIAISVVLGSSWLANRLGARQ

Sequences:

>Translated_276_residues
MRGRAERIGLLALPGLSFLAIIYALPLFMLLLKSFQIDGQFSVEEYVTFFSDEYNLNVLWRTLRVALLTTGLALIIAYPT
AIIMARVKGFWLSVFLVAMVLPMSVGVVVKAFAWTILFRTRGVLNDTLMSLGITEAPIRMLFTETALVIGAANVFLPFMV
LPIYAVLRQMDSRLPEAAASLGATPVFRFMNVTLPLSLPGVVAGSAFTFSLAVSMYVIPSLIVGERQQTLSMLTARSFLY
LRNEALGATISSILLVIAISVVLGSSWLANRLGARQ
>Mature_276_residues
MRGRAERIGLLALPGLSFLAIIYALPLFMLLLKSFQIDGQFSVEEYVTFFSDEYNLNVLWRTLRVALLTTGLALIIAYPT
AIIMARVKGFWLSVFLVAMVLPMSVGVVVKAFAWTILFRTRGVLNDTLMSLGITEAPIRMLFTETALVIGAANVFLPFMV
LPIYAVLRQMDSRLPEAAASLGATPVFRFMNVTLPLSLPGVVAGSAFTFSLAVSMYVIPSLIVGERQQTLSMLTARSFLY
LRNEALGATISSILLVIAISVVLGSSWLANRLGARQ

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=236, Percent_Identity=30.5084745762712, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1787080, Length=226, Percent_Identity=32.3008849557522, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1787713, Length=226, Percent_Identity=34.070796460177, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30206; Mature: 30206

Theoretical pI: Translated: 10.47; Mature: 10.47

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]