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The map label for this gene is gatA [H]

Identifier: 110679655

GI number: 110679655

Start: 2295226

End: 2296563

Strand: Reverse

Name: gatA [H]

Synonym: RD1_2406

Alternate gene names: 110679655

Gene position: 2296563-2295226 (Counterclockwise)

Preceding gene: 110679656

Following gene: 110679654

Centisome position: 55.57

GC content: 64.65

Gene sequence:

>1338_bases
ATGAGCCCTCTCGATGGTCCTATCTCGCAGCTTGTTGCGGCAGTGCAAACCGGCGCAGTCACGCCAAAGGCACTTGTGGC
CGAAGCGCTCGCGCGTGCGGAGGCGCGCAACCCGGCAATCAATGCAATCTGCCAGATCAATCCCCACGCCCTTACGGAGG
CAGAGAATGTGTCCAAAAGGCTGCAAACCGGAGAGACCCTGCCGCTTGCGGGCGTGCCTGTGCTGATCAAAGACAATATC
TGGGTCGCAGGGCTGCGCTGCGCGCAAGGCTCAAAGCTGTTTGCCGATTTCATCGCACCGCAGGATGCCGAAGCGGTGAC
ACGCCTGCGCGTGGCAGGTGCCGTCATCATCGGGATCAGCACCTGTTCCGAATTCGCGTGCAAAGGGGCGACCAACTCCC
CGCTGCAGGGGATCACGCGCAACCCGGTCAACCCGGACCTGACGCCCGGCGGGTCTTCGGGCGGGTCCGTCGCTGCGGTC
GCAGCAGGGATTGTCCCGCTCGCGCTTGGCACGGATGCGGGTGGCTCGTCGCGCCGCCCGCCTGCCCATACGGGTCTGTG
CGGTTTCAAACCGACGCAGGATCTGATCCCCTACGGACCATGCCATGATGAGCCGGTCTGGGGCATCTCAGTCATCTGCC
CCATCGCGCGCCGGATGGAGGACATCAGCCTCGCCATGCAGAGCCTCGCCGGGCTGGAACCTGTGCCGGCCCCGATGCTG
GAGATCGCCGCCTCATCGGATTTCGGCACAGGCCAAGCGCTTGATCGAGATGTGGCAGCAAACTTTGACAAAGTGCTTGG
GGTGCTTGAGGGAGCGGGCGTCTCGGTCACGCAAGCGCAGATTGACTGGCCCGGGACCATCAAGGGACATAGCATCCTGC
CGCTTCAGTGCGCGGGTCTTGCGGATTTATACGGCGATATCTGGCGGGAAACGCCGGAACTGTTCGACCCCGCGATTGCC
GGGCAAATCGAAACGGGCCTGTCATTCACCGGGGTTGACGTGGCCGGGGCGCACCAGATTTCGCACCAGATGCGCGAAAC
ACTGCGGGCCGCGCTGGATCGCTATGGCGTGATTGCCACTCCGACGACACCCTGTGCGGCATGGCCCGCTGCGCAAGACG
CCCCGACCCGGATCGGTGGCACACCCGCAGGACCCCGGGATCATGCCGCCTTCACACCGCAGATCAACCATGCCGGTGTT
CCGGCCCTATCCCTCCCCTGTGGCACGGATGGCGACGGGCGACCGCTTGGCCTGCAACTGATCGCACGCGCGGGCCATGA
CGCGGAACTGATTGCCCTCGCACGGCGGCTGGAACCGCTTCTGAAGGAGATCGCCTGA

Upstream 100 bases:

>100_bases
ATGGTACAGACACTTTTATGAACATGCCCGCCTGCTGCCGTCATGAAAATACTTGGATCGCTTCCGCCACCGGTGGGGCG
CAGTACGAAGGCATGCGGTC

Downstream 100 bases:

>100_bases
CATGCGCCTTTTGCTGATCAACCCGAATACAACGCAAAGCATGACAGCGGCGATGACCACGGTCGCACGACAAATCGCCA
GCCAACAGGCCGAGGTCGTC

Product: glutamyl-tRNA(Gln) amidotransferase, subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MSPLDGPISQLVAAVQTGAVTPKALVAEALARAEARNPAINAICQINPHALTEAENVSKRLQTGETLPLAGVPVLIKDNI
WVAGLRCAQGSKLFADFIAPQDAEAVTRLRVAGAVIIGISTCSEFACKGATNSPLQGITRNPVNPDLTPGGSSGGSVAAV
AAGIVPLALGTDAGGSSRRPPAHTGLCGFKPTQDLIPYGPCHDEPVWGISVICPIARRMEDISLAMQSLAGLEPVPAPML
EIAASSDFGTGQALDRDVAANFDKVLGVLEGAGVSVTQAQIDWPGTIKGHSILPLQCAGLADLYGDIWRETPELFDPAIA
GQIETGLSFTGVDVAGAHQISHQMRETLRAALDRYGVIATPTTPCAAWPAAQDAPTRIGGTPAGPRDHAAFTPQINHAGV
PALSLPCGTDGDGRPLGLQLIARAGHDAELIALARRLEPLLKEIA

Sequences:

>Translated_445_residues
MSPLDGPISQLVAAVQTGAVTPKALVAEALARAEARNPAINAICQINPHALTEAENVSKRLQTGETLPLAGVPVLIKDNI
WVAGLRCAQGSKLFADFIAPQDAEAVTRLRVAGAVIIGISTCSEFACKGATNSPLQGITRNPVNPDLTPGGSSGGSVAAV
AAGIVPLALGTDAGGSSRRPPAHTGLCGFKPTQDLIPYGPCHDEPVWGISVICPIARRMEDISLAMQSLAGLEPVPAPML
EIAASSDFGTGQALDRDVAANFDKVLGVLEGAGVSVTQAQIDWPGTIKGHSILPLQCAGLADLYGDIWRETPELFDPAIA
GQIETGLSFTGVDVAGAHQISHQMRETLRAALDRYGVIATPTTPCAAWPAAQDAPTRIGGTPAGPRDHAAFTPQINHAGV
PALSLPCGTDGDGRPLGLQLIARAGHDAELIALARRLEPLLKEIA
>Mature_444_residues
SPLDGPISQLVAAVQTGAVTPKALVAEALARAEARNPAINAICQINPHALTEAENVSKRLQTGETLPLAGVPVLIKDNIW
VAGLRCAQGSKLFADFIAPQDAEAVTRLRVAGAVIIGISTCSEFACKGATNSPLQGITRNPVNPDLTPGGSSGGSVAAVA
AGIVPLALGTDAGGSSRRPPAHTGLCGFKPTQDLIPYGPCHDEPVWGISVICPIARRMEDISLAMQSLAGLEPVPAPMLE
IAASSDFGTGQALDRDVAANFDKVLGVLEGAGVSVTQAQIDWPGTIKGHSILPLQCAGLADLYGDIWRETPELFDPAIAG
QIETGLSFTGVDVAGAHQISHQMRETLRAALDRYGVIATPTTPCAAWPAAQDAPTRIGGTPAGPRDHAAFTPQINHAGVP
ALSLPCGTDGDGRPLGLQLIARAGHDAELIALARRLEPLLKEIA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=509, Percent_Identity=27.8978388998035, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI195972892, Length=487, Percent_Identity=24.8459958932238, Blast_Score=109, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17543272, Length=424, Percent_Identity=25.9433962264151, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17537465, Length=482, Percent_Identity=24.896265560166, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17556264, Length=192, Percent_Identity=30.2083333333333, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=198, Percent_Identity=32.8282828282828, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17538252, Length=188, Percent_Identity=29.2553191489362, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17556276, Length=193, Percent_Identity=31.0880829015544, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=193, Percent_Identity=31.0880829015544, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=449, Percent_Identity=27.3942093541203, Blast_Score=97, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6323950, Length=173, Percent_Identity=32.3699421965318, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320448, Length=277, Percent_Identity=28.158844765343, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24648435, Length=482, Percent_Identity=26.5560165975104, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24648437, Length=482, Percent_Identity=26.5560165975104, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI45550774, Length=482, Percent_Identity=26.5560165975104, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24644968, Length=493, Percent_Identity=25.3549695740365, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24648113, Length=499, Percent_Identity=28.6573146292585, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24648441, Length=412, Percent_Identity=26.6990291262136, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24648439, Length=412, Percent_Identity=26.6990291262136, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24652985, Length=484, Percent_Identity=26.6528925619835, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19922090, Length=484, Percent_Identity=26.6528925619835, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24652981, Length=484, Percent_Identity=26.6528925619835, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24652983, Length=484, Percent_Identity=26.6528925619835, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21356731, Length=452, Percent_Identity=26.5486725663717, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI161078093, Length=486, Percent_Identity=26.3374485596708, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 45839; Mature: 45708

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPLDGPISQLVAAVQTGAVTPKALVAEALARAEARNPAINAICQINPHALTEAENVSKR
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHH
LQTGETLPLAGVPVLIKDNIWVAGLRCAQGSKLFADFIAPQDAEAVTRLRVAGAVIIGIS
HHCCCCCCCCCCCEEEECCEEEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHCEEEEEEH
TCSEFACKGATNSPLQGITRNPVNPDLTPGGSSGGSVAAVAAGIVPLALGTDAGGSSRRP
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCCCCCCC
PAHTGLCGFKPTQDLIPYGPCHDEPVWGISVICPIARRMEDISLAMQSLAGLEPVPAPML
CCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
EIAASSDFGTGQALDRDVAANFDKVLGVLEGAGVSVTQAQIDWPGTIKGHSILPLQCAGL
HHHCCCCCCCCHHHHHHHHHCHHHHHHHHCCCCCEEEEEEECCCCCCCCCEECCCHHCCH
ADLYGDIWRETPELFDPAIAGQIETGLSFTGVDVAGAHQISHQMRETLRAALDRYGVIAT
HHHHHHHHHCCHHHHCCHHHCHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEC
PTTPCAAWPAAQDAPTRIGGTPAGPRDHAAFTPQINHAGVPALSLPCGTDGDGRPLGLQL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHH
IARAGHDAELIALARRLEPLLKEIA
HHHCCCCHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SPLDGPISQLVAAVQTGAVTPKALVAEALARAEARNPAINAICQINPHALTEAENVSKR
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHH
LQTGETLPLAGVPVLIKDNIWVAGLRCAQGSKLFADFIAPQDAEAVTRLRVAGAVIIGIS
HHCCCCCCCCCCCEEEECCEEEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHCEEEEEEH
TCSEFACKGATNSPLQGITRNPVNPDLTPGGSSGGSVAAVAAGIVPLALGTDAGGSSRRP
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCCCCCCC
PAHTGLCGFKPTQDLIPYGPCHDEPVWGISVICPIARRMEDISLAMQSLAGLEPVPAPML
CCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
EIAASSDFGTGQALDRDVAANFDKVLGVLEGAGVSVTQAQIDWPGTIKGHSILPLQCAGL
HHHCCCCCCCCHHHHHHHHHCHHHHHHHHCCCCCEEEEEEECCCCCCCCCEECCCHHCCH
ADLYGDIWRETPELFDPAIAGQIETGLSFTGVDVAGAHQISHQMRETLRAALDRYGVIAT
HHHHHHHHHCCHHHHCCHHHCHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEC
PTTPCAAWPAAQDAPTRIGGTPAGPRDHAAFTPQINHAGVPALSLPCGTDGDGRPLGLQL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHH
IARAGHDAELIALARRLEPLLKEIA
HHHCCCCHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]