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The map label for this gene is phnF [C]

Identifier: 110679644

GI number: 110679644

Start: 2283558

End: 2284226

Strand: Direct

Name: phnF [C]

Synonym: RD1_2395

Alternate gene names: 110679644

Gene position: 2283558-2284226 (Clockwise)

Preceding gene: 110679634

Following gene: 110679646

Centisome position: 55.25

GC content: 63.38

Gene sequence:

>669_bases
TTGCGCAGCGACTTGGCCGAAGGGCGCTATAAAACGGGTGACAAACTGCCGACCGAGGCTGCGCTGGCGGAGCGCTTTGG
CGTGAACCGTCACACGGTGCGCCACGGGATATCCGCATTGGTTGCGGAAGGGCTGGTGCGCACTCGGCGCGGTGCAGGGG
CCTTTGTCGCGGCCACGCCAACCGACTATCCCATCGGCAAGCGGGTGCGTTTCCACGAAAACCTGATCGCAGCAGGGCGC
CGGCCAGAAAAGCGCGTGCTCGCGTTGGACGAGCGCGCAGCGACCGTTGGCGAGGCGCAGGCGTTGCAGATTGAGGCAGG
CGATCCGGTCTGTGCCTATCACGGGCTTTCACTGGCTGATGGTCAGCCGATTGCAGTGTTTGAAAGCCTGTTTCCGCTGG
CGCGCCTGTTGGGCATCACCGCTGCTTTGAAGGACACAAGCAGCGTCACGCAGGCATTGAGCGTTGTGGGCATCGACGAC
TACACACGTGCCTCAACCCGCCTGACGGCGGTGCGCGCCACGCCAACCTTAGCGCTGCTCTTGCATGTTGCCGAAGGCGA
TCCGCTGCTGAAATCCTCCGGTGTGAATAACTGCGCGGGCGGGCAACCCGTCGAATACGGCCGGACCTGGTTTGCGGGGG
ATAGGGTAACGCTCACGCTTGAAACCTGA

Upstream 100 bases:

>100_bases
ACATATTCTACCATATCCCCAACTTTATGACAGAAGAGATTTGTGAATTTTGCGTGACAGCTCTGACAAAACCCCGATCT
GGAAGGCCATCGCGAGCGCC

Downstream 100 bases:

>100_bases
GGGCCAATCATCCTGACGGGTGGCAGGTGTTGTGGATCGCCTGATCCGTTTCACGCCCATGATCGTCAGGACCGCCTGTC
ACAAAAGGTTCACTTTCCAT

Product: phosphonates metabolism transcriptional regulator PhnF, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MRSDLAEGRYKTGDKLPTEAALAERFGVNRHTVRHGISALVAEGLVRTRRGAGAFVAATPTDYPIGKRVRFHENLIAAGR
RPEKRVLALDERAATVGEAQALQIEAGDPVCAYHGLSLADGQPIAVFESLFPLARLLGITAALKDTSSVTQALSVVGIDD
YTRASTRLTAVRATPTLALLLHVAEGDPLLKSSGVNNCAGGQPVEYGRTWFAGDRVTLTLET

Sequences:

>Translated_222_residues
MRSDLAEGRYKTGDKLPTEAALAERFGVNRHTVRHGISALVAEGLVRTRRGAGAFVAATPTDYPIGKRVRFHENLIAAGR
RPEKRVLALDERAATVGEAQALQIEAGDPVCAYHGLSLADGQPIAVFESLFPLARLLGITAALKDTSSVTQALSVVGIDD
YTRASTRLTAVRATPTLALLLHVAEGDPLLKSSGVNNCAGGQPVEYGRTWFAGDRVTLTLET
>Mature_222_residues
MRSDLAEGRYKTGDKLPTEAALAERFGVNRHTVRHGISALVAEGLVRTRRGAGAFVAATPTDYPIGKRVRFHENLIAAGR
RPEKRVLALDERAATVGEAQALQIEAGDPVCAYHGLSLADGQPIAVFESLFPLARLLGITAALKDTSSVTQALSVVGIDD
YTRASTRLTAVRATPTLALLLHVAEGDPLLKSSGVNNCAGGQPVEYGRTWFAGDRVTLTLET

Specific function: Belongs To An Operon Involved In Alkylphosphonate Uptake And C-P Lyase. Exact Function Not Known. By Similarity Could Be A Transcriptional Regulator. [C]

COG id: COG2188

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790540, Length=214, Percent_Identity=33.6448598130841, Blast_Score=97, Evalue=6e-22,
Organism=Escherichia coli, GI1786950, Length=222, Percent_Identity=27.9279279279279, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI87082252, Length=226, Percent_Identity=24.7787610619469, Blast_Score=77, Evalue=7e-16,
Organism=Escherichia coli, GI1790118, Length=210, Percent_Identity=25.7142857142857, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012702
- InterPro:   IPR000524
- InterPro:   IPR011663
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00392 GntR; PF07702 UTRA [H]

EC number: NA

Molecular weight: Translated: 23615; Mature: 23615

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSDLAEGRYKTGDKLPTEAALAERFGVNRHTVRHGISALVAEGLVRTRRGAGAFVAATP
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
TDYPIGKRVRFHENLIAAGRRPEKRVLALDERAATVGEAQALQIEAGDPVCAYHGLSLAD
CCCCCCCEEEHHHHHHHCCCCCCHHEEEEHHHHHHCCCCEEEEEECCCCEEEECCCCCCC
GQPIAVFESLFPLARLLGITAALKDTSSVTQALSVVGIDDYTRASTRLTAVRATPTLALL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEEEECCCCEEEE
LHVAEGDPLLKSSGVNNCAGGQPVEYGRTWFAGDRVTLTLET
EEECCCCCCHHHCCCCCCCCCCCHHCCCEEECCCEEEEEEEC
>Mature Secondary Structure
MRSDLAEGRYKTGDKLPTEAALAERFGVNRHTVRHGISALVAEGLVRTRRGAGAFVAATP
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
TDYPIGKRVRFHENLIAAGRRPEKRVLALDERAATVGEAQALQIEAGDPVCAYHGLSLAD
CCCCCCCEEEHHHHHHHCCCCCCHHEEEEHHHHHHCCCCEEEEEECCCCEEEECCCCCCC
GQPIAVFESLFPLARLLGITAALKDTSSVTQALSVVGIDDYTRASTRLTAVRATPTLALL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEEEECCCCEEEE
LHVAEGDPLLKSSGVNNCAGGQPVEYGRTWFAGDRVTLTLET
EEECCCCCCHHHCCCCCCCCCCCHHCCCEEECCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481431; 9889346 [H]