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The map label for this gene is bioA [C]

Identifier: 110679592

GI number: 110679592

Start: 2235978

End: 2237336

Strand: Direct

Name: bioA [C]

Synonym: RD1_2337

Alternate gene names: 110679592

Gene position: 2235978-2237336 (Clockwise)

Preceding gene: 110679590

Following gene: 110679594

Centisome position: 54.1

GC content: 58.5

Gene sequence:

>1359_bases
ATGCGCCCCAATTCGATAGAAGCCCGTGATGTTGCCTATCATCTGCATGGCTACACCAACCCCAGGGCACATGAAGAGCA
GGGTCCGATGGTGATCGAGCGTGGCGAGGGGGTCTATGTCTTTGACAATCAGGGCAAGCGCTACATCGAAGGGATGGCCG
GTCTGTGGAGCGTCGCGGTGGGGTTCAACGAAAGCCGCCTTGTGGACGCCGCGCAGAACCAGATGCGCGCGCTGCCCTAT
TACCATAGCTTTTCGCATAAATCGCACGGACCCGCGATTGATCTGGCGGAGCGGTTGATCGACATGGCACCGGTGCAGAT
GAGCAAGGTGTTTTTCACCAACTCGGGGTCCGAAGCGAATGACACGATCCTCAAGATGATCTGGTATCGCTCGAACGCGC
TTGGCAAACCTGAGAAGAAGAAGGTGATTTCACGCCTGCGCGGCTATCATGGCGTGACCATTGCATCGGCGTCCATGACC
GGGTTGCCGAACAATCACCGCTCGTTCGATCTGCCGATTGAGGGCGTGTTGCACACCACATGCCCGCATTACTGGAAGGA
GGGGCGGGACGGCGAAAGTGAAGAGGCATTCGCGACCCGCTGTGCTGAGGATCTGGACGCGATGATCCAGTCCGAGGGGC
CCGACACAATCGCCGCCTTCATTGCCGAACCGATCATGGGGGCAGGGGGCGTCGTGGTGCCACCGGCGACCTATTGGCAA
AAGATACAGGGGGTTCTGGCGAAATATGACATCCTGCTGATCGCGGATGAGGTGATTTGTGGCTTTGGTCGCACCGGCAA
GATGTTCGGATCAAACACCTATGACATCAAACCGGACATCATGACCATGTCCAAGCAACTGACCTCGTCCTATTTCCCGT
TCTCGGCCTTCATGATGAATGAGCGGGTCTATGAGCCCATCGCGGATGAGGGTAACCGCATCGGTATTCTCGGCCATGGC
TATACCGCGAGCGCGCATCCGGTGGGCGCGGCGGTTGCGCTGGAAAACCTCAAGATCATCGAAGAGCGTGACCTCGTCGG
CAATGCAGCCGAACGTGGCGCGGAATTGCAGGCGGGACTGGCGCGCTTCGCCGATCATCCGTTGGTGGGTGAAACCCGCG
GTGTTGGCCTGATCGCAGCGCTTGAGCTTGTCGCGCCCGAGGGGGCGGGCGATGCGCGTGCGCCGGGGGCGCTTGGACTG
CATATGAACCGGATTTTCCACAAGAACGGGTTGATTTCGCGCAATGTGATGGATGCAATGGCATTTTGCCCGCCGCTGAT
CATCACGCAGCAACAGGTTTCCGACATGGTGGATATCGTTGAGAAAAGCCTTGAAACCTTCGCATCGGAGGTGGCGTGA

Upstream 100 bases:

>100_bases
CCCGCCCGCGAACCGCTAGCAATGATTGGAACAGGTCAGTGCGTTTTCATGCCATTGGCTTTTGATTCTGGATGACAATA
AAAGGTTGAGCCTGATGGAA

Downstream 100 bases:

>100_bases
TCACGCCAGATGCTGCCCGCCGTTGATCGGGATTTCAGCGCCGTTCACGTAGGATGATGCCTTTGAGCACAGGAAAGCCA
CCACATCCGCAACCTCTCCG

Product: aminotransferase

Products: S-Adenosyl-4-methylthio-2-oxobutanoate; 7,8-Diaminononanoate [C]

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MRPNSIEARDVAYHLHGYTNPRAHEEQGPMVIERGEGVYVFDNQGKRYIEGMAGLWSVAVGFNESRLVDAAQNQMRALPY
YHSFSHKSHGPAIDLAERLIDMAPVQMSKVFFTNSGSEANDTILKMIWYRSNALGKPEKKKVISRLRGYHGVTIASASMT
GLPNNHRSFDLPIEGVLHTTCPHYWKEGRDGESEEAFATRCAEDLDAMIQSEGPDTIAAFIAEPIMGAGGVVVPPATYWQ
KIQGVLAKYDILLIADEVICGFGRTGKMFGSNTYDIKPDIMTMSKQLTSSYFPFSAFMMNERVYEPIADEGNRIGILGHG
YTASAHPVGAAVALENLKIIEERDLVGNAAERGAELQAGLARFADHPLVGETRGVGLIAALELVAPEGAGDARAPGALGL
HMNRIFHKNGLISRNVMDAMAFCPPLIITQQQVSDMVDIVEKSLETFASEVA

Sequences:

>Translated_452_residues
MRPNSIEARDVAYHLHGYTNPRAHEEQGPMVIERGEGVYVFDNQGKRYIEGMAGLWSVAVGFNESRLVDAAQNQMRALPY
YHSFSHKSHGPAIDLAERLIDMAPVQMSKVFFTNSGSEANDTILKMIWYRSNALGKPEKKKVISRLRGYHGVTIASASMT
GLPNNHRSFDLPIEGVLHTTCPHYWKEGRDGESEEAFATRCAEDLDAMIQSEGPDTIAAFIAEPIMGAGGVVVPPATYWQ
KIQGVLAKYDILLIADEVICGFGRTGKMFGSNTYDIKPDIMTMSKQLTSSYFPFSAFMMNERVYEPIADEGNRIGILGHG
YTASAHPVGAAVALENLKIIEERDLVGNAAERGAELQAGLARFADHPLVGETRGVGLIAALELVAPEGAGDARAPGALGL
HMNRIFHKNGLISRNVMDAMAFCPPLIITQQQVSDMVDIVEKSLETFASEVA
>Mature_452_residues
MRPNSIEARDVAYHLHGYTNPRAHEEQGPMVIERGEGVYVFDNQGKRYIEGMAGLWSVAVGFNESRLVDAAQNQMRALPY
YHSFSHKSHGPAIDLAERLIDMAPVQMSKVFFTNSGSEANDTILKMIWYRSNALGKPEKKKVISRLRGYHGVTIASASMT
GLPNNHRSFDLPIEGVLHTTCPHYWKEGRDGESEEAFATRCAEDLDAMIQSEGPDTIAAFIAEPIMGAGGVVVPPATYWQ
KIQGVLAKYDILLIADEVICGFGRTGKMFGSNTYDIKPDIMTMSKQLTSSYFPFSAFMMNERVYEPIADEGNRIGILGHG
YTASAHPVGAAVALENLKIIEERDLVGNAAERGAELQAGLARFADHPLVGETRGVGLIAALELVAPEGAGDARAPGALGL
HMNRIFHKNGLISRNVMDAMAFCPPLIITQQQVSDMVDIVEKSLETFASEVA

Specific function: Biotin biosynthesis. [C]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI37574042, Length=430, Percent_Identity=31.1627906976744, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI13994255, Length=440, Percent_Identity=27.0454545454545, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI226442705, Length=430, Percent_Identity=31.3953488372093, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI4557809, Length=449, Percent_Identity=25.3897550111359, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI226442709, Length=392, Percent_Identity=31.6326530612245, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI24119277, Length=433, Percent_Identity=28.4064665127021, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI284507298, Length=338, Percent_Identity=26.0355029585799, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI188536080, Length=396, Percent_Identity=25.7575757575758, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI38679950, Length=396, Percent_Identity=25.7575757575758, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI38679946, Length=396, Percent_Identity=25.7575757575758, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1786991, Length=448, Percent_Identity=29.2410714285714, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI145693181, Length=412, Percent_Identity=27.9126213592233, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1789759, Length=430, Percent_Identity=27.2093023255814, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788044, Length=425, Percent_Identity=27.7647058823529, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1789016, Length=436, Percent_Identity=25.9174311926606, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1787560, Length=419, Percent_Identity=27.6849642004773, Blast_Score=113, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32564660, Length=463, Percent_Identity=30.2375809935205, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI25144271, Length=426, Percent_Identity=27.2300469483568, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI25144274, Length=263, Percent_Identity=31.1787072243346, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71992977, Length=370, Percent_Identity=24.8648648648649, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17541228, Length=481, Percent_Identity=23.0769230769231, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323470, Length=430, Percent_Identity=25.1162790697674, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324432, Length=447, Percent_Identity=25.2796420581655, Blast_Score=119, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6324386, Length=436, Percent_Identity=25.2293577981651, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321456, Length=321, Percent_Identity=28.6604361370716, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21357415, Length=451, Percent_Identity=27.2727272727273, Blast_Score=148, Evalue=9e-36,
Organism=Drosophila melanogaster, GI161085790, Length=465, Percent_Identity=27.5268817204301, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI28574759, Length=455, Percent_Identity=28.1318681318681, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI21356575, Length=362, Percent_Identity=27.9005524861878, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI281366494, Length=451, Percent_Identity=24.8337028824834, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24667139, Length=451, Percent_Identity=24.8337028824834, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24667143, Length=451, Percent_Identity=24.8337028824834, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.62 [C]

Molecular weight: Translated: 49387; Mature: 49387

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPNSIEARDVAYHLHGYTNPRAHEEQGPMVIERGEGVYVFDNQGKRYIEGMAGLWSVAV
CCCCCCCHHHEEEEECCCCCCCCCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHHHHHC
GFNESRLVDAAQNQMRALPYYHSFSHKSHGPAIDLAERLIDMAPVQMSKVFFTNSGSEAN
CCCHHHHHHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCHH
DTILKMIWYRSNALGKPEKKKVISRLRGYHGVTIASASMTGLPNNHRSFDLPIEGVLHTT
HHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEECEECCCCCCCCEECCCHHHHHHHC
CPHYWKEGRDGESEEAFATRCAEDLDAMIQSEGPDTIAAFIAEPIMGAGGVVVPPATYWQ
CCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEECCHHHHH
KIQGVLAKYDILLIADEVICGFGRTGKMFGSNTYDIKPDIMTMSKQLTSSYFPFSAFMMN
HHHHHHHHHHEEEEEHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
ERVYEPIADEGNRIGILGHGYTASAHPVGAAVALENLKIIEERDLVGNAAERGAELQAGL
HHHHCHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHH
ARFADHPLVGETRGVGLIAALELVAPEGAGDARAPGALGLHMNRIFHKNGLISRNVMDAM
HHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHH
AFCPPLIITQQQVSDMVDIVEKSLETFASEVA
HHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRPNSIEARDVAYHLHGYTNPRAHEEQGPMVIERGEGVYVFDNQGKRYIEGMAGLWSVAV
CCCCCCCHHHEEEEECCCCCCCCCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHHHHHC
GFNESRLVDAAQNQMRALPYYHSFSHKSHGPAIDLAERLIDMAPVQMSKVFFTNSGSEAN
CCCHHHHHHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCHH
DTILKMIWYRSNALGKPEKKKVISRLRGYHGVTIASASMTGLPNNHRSFDLPIEGVLHTT
HHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEECEECCCCCCCCEECCCHHHHHHHC
CPHYWKEGRDGESEEAFATRCAEDLDAMIQSEGPDTIAAFIAEPIMGAGGVVVPPATYWQ
CCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEECCHHHHH
KIQGVLAKYDILLIADEVICGFGRTGKMFGSNTYDIKPDIMTMSKQLTSSYFPFSAFMMN
HHHHHHHHHHEEEEEHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
ERVYEPIADEGNRIGILGHGYTASAHPVGAAVALENLKIIEERDLVGNAAERGAELQAGL
HHHHCHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHH
ARFADHPLVGETRGVGLIAALELVAPEGAGDARAPGALGLHMNRIFHKNGLISRNVMDAM
HHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHH
AFCPPLIITQQQVSDMVDIVEKSLETFASEVA
HHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 8-Amino-7-oxononanoate; S-Adenosyl-L-methionine [C]

Specific reaction: 8-Amino-7-oxononanoate + S-Adenosyl-L-methionine <==> S-Adenosyl-4-methylthio-2-oxobutanoate + 7,8-Diaminononanoate [C]

General reaction: Amino group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424 [H]