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The map label for this gene is dmdA [H]

Identifier: 110679550

GI number: 110679550

Start: 2182262

End: 2183365

Strand: Direct

Name: dmdA [H]

Synonym: RD1_2288

Alternate gene names: 110679550

Gene position: 2182262-2183365 (Clockwise)

Preceding gene: 110679549

Following gene: 110679551

Centisome position: 52.8

GC content: 61.96

Gene sequence:

>1104_bases
ATGGCGCTTATCTCTCCGTCGCGCAGGCTGCGCCGCACCCCTTTTTCCGATGGTGTGGAGGCCGCAGGGGTCAAGGCCTA
CACGGTCTACAACCGCATGTTGCTGCCCACCGTCTTTGAAAGTGTGGAGGCTGACTACCGTCACCTCAAGGAACATGTGC
AGGTCTGGGATGTTTCGGTCGAGCGTCAGGTCGAACTGCGTGGCCCGGATGCCGCCCGCCTGATGCAGATGCTGACCCCG
CGCGATCTGCGCGGCATGCTGCCGGGGCGGTGCTTTTACGTGCCCATCGTGGATGAGACGGGTGGCATGCTGAACGACCC
CGTGGCGGTCAAGCTGGCCGAAGACCGCTGGTGGATTTCCATTGCGGACAGTGATCTGCTGTTGTGGGTCAAGGGGATTT
CCAATGGCTATCGACTGGATGTGCTGATTGATGAACCGGATGTCAGCCCGCTGGCCATTCAGGGGCCAAAGGCGGACGAA
CTGGCCGCCCGTATCTTTGGCGACAGCATCCGCGACATCAAGTTTTTCCGCTTTGGTCTCTTTGAATTCGAGGGGCGTGA
GATGGTCGTGGCGCGGTCGGGGTATTCCAAACAAGGCGGTTTTGAGGTCTATGTCGAGGGGTCGGATATCGCGATGCCGC
TCTGGGATGCGCTGTTCAAGGCGGGCGAGGACCTGAACGTGCGCGCAGGGTGCCCCAATGGCATTGAGCGGATCGAGGGC
GGTCTGCTGTCTTATGGCAACGACATGACTGACGACAACACGCCGCATGAATGCGGGCTTGGGCGGTTTTGTGATACGCA
GACCGCTATTGGCTGCATCGGTCGGGATGCCTTGCTGCGCGTGGCCAAGGAAGGACCGGTGCAACAGATCAGGGCGATTG
CGATCGAGGGCGACCGGCTGCCGCTCTGTGACCGGGCCTGGGGGCTTTACGCCGGGGATACGCGCGTTGGGCAGGTCACT
TCCGCTGCCTGGAGCCCGGATCACCACACCAATGTCGCCATCGGCATGGTGCGCATGACGCATTGGGATACCGGCACAAG
GCTGCGCGTTGAAACGCCGGATGGCGCGCGCGATGCGACCGTGCAGGCCGGTTTCTGGCACTGA

Upstream 100 bases:

>100_bases
CACCAGACCCATCACCGTGGTCAGATCCATGCGATGCTGACCGCTTCGGGGCGCAAAGCTCCGGTAAGTGATCTGGTCTT
CATGCCACAGGATGCTTGAT

Downstream 100 bases:

>100_bases
TGGTTTTTGCTTTTGCCGGGCTTTCCGGCAGGGGCGTTCAACACCAACGACAGGGGCAAGTCCCCGGATAGGAGAGAGAC
ATGTTTAAGGCGCTTGTGGT

Product: putative dimethyl sulfoniopropionate demethylase

Products: NA

Alternate protein names: Glycine cleavage system T protein [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MALISPSRRLRRTPFSDGVEAAGVKAYTVYNRMLLPTVFESVEADYRHLKEHVQVWDVSVERQVELRGPDAARLMQMLTP
RDLRGMLPGRCFYVPIVDETGGMLNDPVAVKLAEDRWWISIADSDLLLWVKGISNGYRLDVLIDEPDVSPLAIQGPKADE
LAARIFGDSIRDIKFFRFGLFEFEGREMVVARSGYSKQGGFEVYVEGSDIAMPLWDALFKAGEDLNVRAGCPNGIERIEG
GLLSYGNDMTDDNTPHECGLGRFCDTQTAIGCIGRDALLRVAKEGPVQQIRAIAIEGDRLPLCDRAWGLYAGDTRVGQVT
SAAWSPDHHTNVAIGMVRMTHWDTGTRLRVETPDGARDATVQAGFWH

Sequences:

>Translated_367_residues
MALISPSRRLRRTPFSDGVEAAGVKAYTVYNRMLLPTVFESVEADYRHLKEHVQVWDVSVERQVELRGPDAARLMQMLTP
RDLRGMLPGRCFYVPIVDETGGMLNDPVAVKLAEDRWWISIADSDLLLWVKGISNGYRLDVLIDEPDVSPLAIQGPKADE
LAARIFGDSIRDIKFFRFGLFEFEGREMVVARSGYSKQGGFEVYVEGSDIAMPLWDALFKAGEDLNVRAGCPNGIERIEG
GLLSYGNDMTDDNTPHECGLGRFCDTQTAIGCIGRDALLRVAKEGPVQQIRAIAIEGDRLPLCDRAWGLYAGDTRVGQVT
SAAWSPDHHTNVAIGMVRMTHWDTGTRLRVETPDGARDATVQAGFWH
>Mature_366_residues
ALISPSRRLRRTPFSDGVEAAGVKAYTVYNRMLLPTVFESVEADYRHLKEHVQVWDVSVERQVELRGPDAARLMQMLTPR
DLRGMLPGRCFYVPIVDETGGMLNDPVAVKLAEDRWWISIADSDLLLWVKGISNGYRLDVLIDEPDVSPLAIQGPKADEL
AARIFGDSIRDIKFFRFGLFEFEGREMVVARSGYSKQGGFEVYVEGSDIAMPLWDALFKAGEDLNVRAGCPNGIERIEGG
LLSYGNDMTDDNTPHECGLGRFCDTQTAIGCIGRDALLRVAKEGPVQQIRAIAIEGDRLPLCDRAWGLYAGDTRVGQVTS
AAWSPDHHTNVAIGMVRMTHWDTGTRLRVETPDGARDATVQAGFWH

Specific function: The glycine cleavage system catalyzes the degradation of glycine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family [H]

Homologues:

Organism=Homo sapiens, GI257796258, Length=357, Percent_Identity=28.8515406162465, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI44662838, Length=353, Percent_Identity=28.6118980169972, Blast_Score=98, Evalue=9e-21,
Organism=Homo sapiens, GI257796256, Length=299, Percent_Identity=28.0936454849498, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1789272, Length=318, Percent_Identity=22.6415094339623, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320222, Length=341, Percent_Identity=24.3401759530792, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI20129441, Length=343, Percent_Identity=26.2390670553936, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903 [H]

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C [H]

EC number: =2.1.2.10 [H]

Molecular weight: Translated: 40786; Mature: 40655

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALISPSRRLRRTPFSDGVEAAGVKAYTVYNRMLLPTVFESVEADYRHLKEHVQVWDVSV
CCCCCCCHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHEEEECEE
ERQVELRGPDAARLMQMLTPRDLRGMLPGRCFYVPIVDETGGMLNDPVAVKLAEDRWWIS
EEEEEECCCCHHHHHHHHCCHHHHCCCCCCEEEEEEECCCCCCCCCCEEEEEECCEEEEE
IADSDLLLWVKGISNGYRLDVLIDEPDVSPLAIQGPKADELAARIFGDSIRDIKFFRFGL
EECCCEEEEEEECCCCEEEEEEECCCCCCCEEEECCCHHHHHHHHHCCCHHHEEEEEEEE
FEFEGREMVVARSGYSKQGGFEVYVEGSDIAMPLWDALFKAGEDLNVRAGCPNGIERIEG
EEECCCEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEECCCCCHHHHHCC
GLLSYGNDMTDDNTPHECGLGRFCDTQTAIGCIGRDALLRVAKEGPVQQIRAIAIEGDRL
CCEECCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHCCCCHHHEEEEEECCCCC
PLCDRAWGLYAGDTRVGQVTSAAWSPDHHTNVAIGMVRMTHWDTGTRLRVETPDGARDAT
CCHHHCCCEEECCCCCCEEEECCCCCCCCCCEEEEEEEEEEECCCCEEEEECCCCCCCCE
VQAGFWH
EECCCCC
>Mature Secondary Structure 
ALISPSRRLRRTPFSDGVEAAGVKAYTVYNRMLLPTVFESVEADYRHLKEHVQVWDVSV
CCCCCCHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHEEEECEE
ERQVELRGPDAARLMQMLTPRDLRGMLPGRCFYVPIVDETGGMLNDPVAVKLAEDRWWIS
EEEEEECCCCHHHHHHHHCCHHHHCCCCCCEEEEEEECCCCCCCCCCEEEEEECCEEEEE
IADSDLLLWVKGISNGYRLDVLIDEPDVSPLAIQGPKADELAARIFGDSIRDIKFFRFGL
EECCCEEEEEEECCCCEEEEEEECCCCCCCEEEECCCHHHHHHHHHCCCHHHEEEEEEEE
FEFEGREMVVARSGYSKQGGFEVYVEGSDIAMPLWDALFKAGEDLNVRAGCPNGIERIEG
EEECCCEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEECCCCCHHHHHCC
GLLSYGNDMTDDNTPHECGLGRFCDTQTAIGCIGRDALLRVAKEGPVQQIRAIAIEGDRL
CCEECCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHCCCCHHHEEEEEECCCCC
PLCDRAWGLYAGDTRVGQVTSAAWSPDHHTNVAIGMVRMTHWDTGTRLRVETPDGARDAT
CCHHHCCCEEECCCCCCEEEECCCCCCCCCCEEEEEEEEEEECCCCEEEEECCCCCCCCE
VQAGFWH
EECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]