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The map label for this gene is tusA [H]

Identifier: 110679533

GI number: 110679533

Start: 2170883

End: 2171113

Strand: Reverse

Name: tusA [H]

Synonym: RD1_2268

Alternate gene names: 110679533

Gene position: 2171113-2170883 (Counterclockwise)

Preceding gene: 110679536

Following gene: 110679526

Centisome position: 52.53

GC content: 57.14

Gene sequence:

>231_bases
ATGACAAAAGAAATACACCTGCTGGACGCCACTGGTCTGCTGTGCCCCCTGCCTGTTCTGAAGGCGCGCAAACGACTGCA
ATCCCTTGCTTCGGGTGAGGTGCTGACGATGCACGCGGATGATCCCGCGGCGATTGTCGATGTCCCGCACTTTTGCAGCG
AGACAGGGCACCACCTTGTGTCAAGCGAACTGGATGGGCCGGTGCAGGTCTATGTCATCCGCAAAAAATGA

Upstream 100 bases:

>100_bases
CCGAACATAAACCTCCCTTAGCCTATCCGCGCCACAGCGTCACCCCGCCAAGGGTCACAAGATTGTGGACAGGACGAAAC
ATCGCGCCTATCTGAACGGT

Downstream 100 bases:

>100_bases
GGTGACATGAAAAAGCGCGCCCGATTTGGACGCGCTTTTTGGGTTTTTGGTGTTGGAAAGCCCTTAGCCTCCGATGGACC
ACCACCCGCGCCGCTTTGGT

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein A [H]

Number of amino acids: Translated: 76; Mature: 75

Protein sequence:

>76_residues
MTKEIHLLDATGLLCPLPVLKARKRLQSLASGEVLTMHADDPAAIVDVPHFCSETGHHLVSSELDGPVQVYVIRKK

Sequences:

>Translated_76_residues
MTKEIHLLDATGLLCPLPVLKARKRLQSLASGEVLTMHADDPAAIVDVPHFCSETGHHLVSSELDGPVQVYVIRKK
>Mature_75_residues
TKEIHLLDATGLLCPLPVLKARKRLQSLASGEVLTMHADDPAAIVDVPHFCSETGHHLVSSELDGPVQVYVIRKK

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions. Interacts with iscS and stimulates its activity. Then, accepts a sulfur from iscS and transfers it in turn to tusD [H]

COG id: COG0425

COG function: function code O; Predicted redox protein, regulator of disulfide bond formation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0033 family. TusA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789881, Length=70, Percent_Identity=48.5714285714286, Blast_Score=71, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001455
- InterPro:   IPR022931 [H]

Pfam domain/function: PF01206 SirA [H]

EC number: NA

Molecular weight: Translated: 8298; Mature: 8167

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS01148 UPF0033

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKEIHLLDATGLLCPLPVLKARKRLQSLASGEVLTMHADDPAAIVDVPHFCSETGHHLV
CCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCHHHCCCCCEEE
SSELDGPVQVYVIRKK
CCCCCCCEEEEEEECC
>Mature Secondary Structure 
TKEIHLLDATGLLCPLPVLKARKRLQSLASGEVLTMHADDPAAIVDVPHFCSETGHHLV
CCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCHHHCCCCCEEE
SSELDGPVQVYVIRKK
CCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA