Click here to switch to the map view.

The map label for this gene is tilS [H]

Identifier: 110679379

GI number: 110679379

Start: 2003311

End: 2004555

Strand: Direct

Name: tilS [H]

Synonym: RD1_2100

Alternate gene names: 110679379

Gene position: 2003311-2004555 (Clockwise)

Preceding gene: 110679378

Following gene: 110679380

Centisome position: 48.47

GC content: 63.69

Gene sequence:

>1245_bases
ATGTGCGATCTTGACGCTGAGATCGACGCACATTTTGTGAATGACATCCCGAAAGCACTTGGTGTTGCCGTGTCGGGCGG
CAGTGATTCAATGGCGCTGCTGGTCTTGCTGTGCGGCTTTGCGGAACGCAATGGGGTCGCGCTGTCCTGTGCGACGGTGG
ATCATGGGCTGAGGCCCGAGGCGGCTGACGAGGCGCGGAGCGTTGCAGGATATTGCGCCGCGCATGATGTGCCGCATGTC
ACGCTGAAATGGGAGGGATGGGACGGCACGGGCAATACGCAAAACGCTGCCCGCGTGGCGCGGTATCGGCTGCTGGCGGA
CTGGGCGCGGGGGCAGGGCATTGCGGATATCGCGCTGGGGCACACGCAGGATGATCAGGCCGAAACCGTGCTGATGGGGC
TCGCGCGTGCGTCCGGTGTTGATGGGCTCAGCGCGATGCCGGCACGGCGCGATGATCGGGGCCTGTGCTGGCACCGGCCC
CTCCTCGCTACATCGCGGGCAGGGTTGCGCGGTTTCCTGAGCGCGAATGGTGTGGCGTGGTGCGAGGATCCCAGCAACGA
TGATGCGCAATTCGAAAGGATAAAGGCACGCCGCGCGCTCACGACGTTGGCGGAACTGGGGGTGGATCGCGCATCCCTCG
CGCAGGTTGCACAGAATATGGCACAGGCGCGGGCAGCACTGGAGCAGCAGACGCGGCGGGCGATTGGTGATCTTGTCAGC
CTGCTTGGCGGTGGTGCGATGATGCCATGGTCCGGCTTTCAGACCCTGCCGAGCGAGATTGCGCGCCGGGTGCTGATGGC
CGCCATCGACTGGATCGCCGCGCCGCCCCATGCGCCGCGACAAAAAGCCCTCACCGCCGCTCTGTCGGCGTTAAGGGCCA
GCGGCAGCGCAACAGTGGCGGGCTGTCGTTTGATCCTCAAGGATGACCGGTTCTGGATGTTCAGGGAATTTCAGGCCGTG
GCGCATGCGCGTGCAGATGCATCACAGCTGTGGGATAATCGCTGGAAAGTCACCGGTGGTCAGGGCAAACAGGCAAGAGA
AATCAGGGCCTTGGGTGAAATTGGTCTGCAACAGCACCCGGAGTGGCGAACCATGGGCCTGCCGCGTGCACTCTTGCTCG
CCACGCCGGCTGTTTACCAGGGCGACGTCCTGATCGCGGCACCGGTGGTGCAAAACACGGCACGATGGCGCGCGGACGTG
ATAAAAAGGCCGGGCTGCCTTTTTGATGAGGTATTCGGCCATTGA

Upstream 100 bases:

>100_bases
CTGCGTCACCCTAGCCGAGGTGGGCATCCGCTTTGCCGGTGATCCAGCGGTGGCGCAAGCCGAAGAGGCCCGGCGCGCGC
TGGCCTGTCAATAGCACGTC

Downstream 100 bases:

>100_bases
ACATACTGGTGGTACGTCTATCTTAAGACTGTGGCTTTATCATGCGGATCAAGACCACAAACGGTATTAGGAGGTTTTCT
TTGGGAAACGCACGCAACAT

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MCDLDAEIDAHFVNDIPKALGVAVSGGSDSMALLVLLCGFAERNGVALSCATVDHGLRPEAADEARSVAGYCAAHDVPHV
TLKWEGWDGTGNTQNAARVARYRLLADWARGQGIADIALGHTQDDQAETVLMGLARASGVDGLSAMPARRDDRGLCWHRP
LLATSRAGLRGFLSANGVAWCEDPSNDDAQFERIKARRALTTLAELGVDRASLAQVAQNMAQARAALEQQTRRAIGDLVS
LLGGGAMMPWSGFQTLPSEIARRVLMAAIDWIAAPPHAPRQKALTAALSALRASGSATVAGCRLILKDDRFWMFREFQAV
AHARADASQLWDNRWKVTGGQGKQAREIRALGEIGLQQHPEWRTMGLPRALLLATPAVYQGDVLIAAPVVQNTARWRADV
IKRPGCLFDEVFGH

Sequences:

>Translated_414_residues
MCDLDAEIDAHFVNDIPKALGVAVSGGSDSMALLVLLCGFAERNGVALSCATVDHGLRPEAADEARSVAGYCAAHDVPHV
TLKWEGWDGTGNTQNAARVARYRLLADWARGQGIADIALGHTQDDQAETVLMGLARASGVDGLSAMPARRDDRGLCWHRP
LLATSRAGLRGFLSANGVAWCEDPSNDDAQFERIKARRALTTLAELGVDRASLAQVAQNMAQARAALEQQTRRAIGDLVS
LLGGGAMMPWSGFQTLPSEIARRVLMAAIDWIAAPPHAPRQKALTAALSALRASGSATVAGCRLILKDDRFWMFREFQAV
AHARADASQLWDNRWKVTGGQGKQAREIRALGEIGLQQHPEWRTMGLPRALLLATPAVYQGDVLIAAPVVQNTARWRADV
IKRPGCLFDEVFGH
>Mature_414_residues
MCDLDAEIDAHFVNDIPKALGVAVSGGSDSMALLVLLCGFAERNGVALSCATVDHGLRPEAADEARSVAGYCAAHDVPHV
TLKWEGWDGTGNTQNAARVARYRLLADWARGQGIADIALGHTQDDQAETVLMGLARASGVDGLSAMPARRDDRGLCWHRP
LLATSRAGLRGFLSANGVAWCEDPSNDDAQFERIKARRALTTLAELGVDRASLAQVAQNMAQARAALEQQTRRAIGDLVS
LLGGGAMMPWSGFQTLPSEIARRVLMAAIDWIAAPPHAPRQKALTAALSALRASGSATVAGCRLILKDDRFWMFREFQAV
AHARADASQLWDNRWKVTGGQGKQAREIRALGEIGLQQHPEWRTMGLPRALLLATPAVYQGDVLIAAPVVQNTARWRADV
IKRPGCLFDEVFGH

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=174, Percent_Identity=36.7816091954023, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 44587; Mature: 44587

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCDLDAEIDAHFVNDIPKALGVAVSGGSDSMALLVLLCGFAERNGVALSCATVDHGLRPE
CCCCCCCHHHHHHHHHHHHHCEEECCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCC
AADEARSVAGYCAAHDVPHVTLKWEGWDGTGNTQNAARVARYRLLADWARGQGIADIALG
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECC
HTQDDQAETVLMGLARASGVDGLSAMPARRDDRGLCWHRPLLATSRAGLRGFLSANGVAW
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEE
CEDPSNDDAQFERIKARRALTTLAELGVDRASLAQVAQNMAQARAALEQQTRRAIGDLVS
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGGGAMMPWSGFQTLPSEIARRVLMAAIDWIAAPPHAPRQKALTAALSALRASGSATVA
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEE
GCRLILKDDRFWMFREFQAVAHARADASQLWDNRWKVTGGQGKQAREIRALGEIGLQQHP
HEEEEEECCCHHHHHHHHHHHHHHCCHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCC
EWRTMGLPRALLLATPAVYQGDVLIAAPVVQNTARWRADVIKRPGCLFDEVFGH
CHHHCCCCHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCHHHHHHCC
>Mature Secondary Structure
MCDLDAEIDAHFVNDIPKALGVAVSGGSDSMALLVLLCGFAERNGVALSCATVDHGLRPE
CCCCCCCHHHHHHHHHHHHHCEEECCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCC
AADEARSVAGYCAAHDVPHVTLKWEGWDGTGNTQNAARVARYRLLADWARGQGIADIALG
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECC
HTQDDQAETVLMGLARASGVDGLSAMPARRDDRGLCWHRPLLATSRAGLRGFLSANGVAW
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEE
CEDPSNDDAQFERIKARRALTTLAELGVDRASLAQVAQNMAQARAALEQQTRRAIGDLVS
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGGGAMMPWSGFQTLPSEIARRVLMAAIDWIAAPPHAPRQKALTAALSALRASGSATVA
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEE
GCRLILKDDRFWMFREFQAVAHARADASQLWDNRWKVTGGQGKQAREIRALGEIGLQQHP
HEEEEEECCCHHHHHHHHHHHHHHCCHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCC
EWRTMGLPRALLLATPAVYQGDVLIAAPVVQNTARWRADVIKRPGCLFDEVFGH
CHHHCCCCHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA