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The map label for this gene is priA [H]

Identifier: 110679362

GI number: 110679362

Start: 1988001

End: 1990163

Strand: Direct

Name: priA [H]

Synonym: RD1_2081

Alternate gene names: 110679362

Gene position: 1988001-1990163 (Clockwise)

Preceding gene: 110679357

Following gene: 110679363

Centisome position: 48.1

GC content: 63.62

Gene sequence:

>2163_bases
TTGATCGCTGTCCTGACGACCCAGCCTCTGGGCCGCACGCTGGATTATCGCGCGCCCGAAGGGGGGTGCCATCTGGGTGC
TTTTGTCGAGGTGCCGCTTGGCCCGCGCAAGGTGCTGGGGGTGGTCTGGGGGCCGGGGCAGGGGGATTATGAACGCTCCA
AAATCCGTCAGGCCTACCGGGTGCTGGATGCCGCTCCCATGCGCGAAGAGATGTGTCTGTTTCTCAAACGCGCGGCGGAG
TATACCCTGACGCCGCTGCCTGCGATGTTGCGTCTTGCCACCCGCGCGCCGGGTCTGGGTGATCCGCCGTCGATGCGCAA
GATTTACCGGCGCGGAACAGGAGAACCTGACAAGATGACGGCGGCCAGGCGCAAGGTTCTGGCGGCCCTGGCGGAATACG
GTGATCTGGCCTTCACGCTCAAGGAACTCGCCGAGATGGCCGATGTGGGTACGTCCGTGGTCAAAGGTCTGGTCGCACAG
GGCGCTGTTGTCGAAGAAGACAGCCCGCGCGATCTGCCCTTTCCCCGTCTTGACCCCTCGCGCCCCGGCAAGGCACTGAC
AGCGGATCAGGCGGCGGGGGCGGCGCAATTGACGCAAGCGGTCCGTTCAGAACGATATGGCACGACCTTGCTGCGTGGTG
TCACGGGTTCGGGCAAGACCGAGGTTTATCTTGAGGCGGTTGCGGCGGCCCTTACGGCGGGCCGGCAGGCGCTGGTGCTC
TTGCCCGAGATCGCCCTGACCGCTGAATTTCTGACCCGGGTGCAGGAGCGCTTTGGTGCGCGCCCGGCTGAATGGCATTC
AGGCGCGACCATGACCGAACGCAGGCGTATCTTTCGCATGGTTGGTCAGGGTGAGGCGCAGCTTGTCATCGGCGCGCGGT
CGGCGCTGTTTCTGCCCTATCAGAACCTTGGCCTGATCGTGGTCGATGAGGAACATGATACTTCCTACAAGCAGGAAGAG
GGGGTTTTGTACAACGCGCGCGATATGGCAGTGCTGCGCGCTTCGATCTGCGGGGCGCAGGTCGTGCTTGCCTCTGCCAC
GCCCTCGCTGGAAAGCTGGGCCAATGCGCAGGCGGGCAAATACGCCAAGCTGGAACTGACCTCGCGGTTCGGGGCCGCTG
TGATGCCGCAGATGCGGGCCATTGATATGCGCGTTGAAAGCCTTCCGTCCAATCGTTGGGTCTCTGATGTGCTGCACAAA
GAGGTGGAGGCACGCATGGCGCGGGGGGAGCAGTCGATGCTGTTCATCAACCGGCGCGGCTATGCCCCGATCACGCTGTG
TCGCGCCTGTGGCCATCAAATCGGCTGCGATCATTGCGACGCGCGCATGGTGGAGCACCGCTTTCTCAAGCGCCTTGTCT
GTCACCAGTGCGGCGAAAGCAAACCGATGCCGACGGTCTGCCCGTCCTGCAATGCCGAAGACAGACTGGCCGCGGTCGGG
CCCGGTGTCGAGCGGCTCGCAGAAGAGGCGACAGAATTGTTCCCCGACGCCCGGATCGCCGCGCTCAGTTCGGATATGTA
TGGTTCCGCCCGCGCGCTCAAGGCGGAGATCGAAGATATCGCGGCGGGCGGTGCGGACATCATCATCGGCACGCAGCTCG
TGGCCAAGGGGCACAATTTTCCTAACCTGACGCTGGTTGGTGTGATTGATGCGGATCTGGGCCTGCAAGGATCTGATCTG
CGCGCTGCCGAACGGACGTTTCAATTGATGCGGCAGGTGGCGGGCCGTGCAGGGCGTGCCGAGAAAGCAGGCGTGGCCCT
GTTGCAAAGCTATCAACCCGAACATCCCGTCATCCGCGCCATTCTGGCCGGGGACGAAGATGAATTCTGGGCTGCCGAGG
CGCGCGAGCGCAAGGCCGCAGGTGTGCCACCTTACGGGCGCATGGCGGGTATCATCCTCAGCGGGTCGGACGTGGCAATG
GTGTTTGACGCAGGCAACCAACTGGCGCGGCAGGATGCGCCGTTGCGGGCCATCGGTGCGCAGGTTTTCGGACCAGCGCC
TGCGCCGATTGCGCGGGTCCGCGGGCGCCATAGGGTCCGGATGCTTGTAAAGGCCGAGAAATCCGCCCCTTTGCAGGAAG
CGCTCGCGCGTTGGGTGGCCCAATTGCGGATCAAAGGTGATTTACGTGTCGCCGTTGATATCGACCCACAGAGTTTCTAC
TGA

Upstream 100 bases:

>100_bases
GCTTGGCTTTACCGCATGATCTGCGATGCTGAAACCCTCAATTGCCGTTTCAGCCGGAGCCTGCCCTTTGAGCCTGCCGC
AATTTTACGATTGTGATGCG

Downstream 100 bases:

>100_bases
CCTTCACCGTCATCATGCGGGGCATCAGGGACATTGACGCGCCGCGAAATAGCACCTCGTCAAAGGCCAGCAAAAGGACT
AAGCAACCGGTATGGCCCGC

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 720; Mature: 720

Protein sequence:

>720_residues
MIAVLTTQPLGRTLDYRAPEGGCHLGAFVEVPLGPRKVLGVVWGPGQGDYERSKIRQAYRVLDAAPMREEMCLFLKRAAE
YTLTPLPAMLRLATRAPGLGDPPSMRKIYRRGTGEPDKMTAARRKVLAALAEYGDLAFTLKELAEMADVGTSVVKGLVAQ
GAVVEEDSPRDLPFPRLDPSRPGKALTADQAAGAAQLTQAVRSERYGTTLLRGVTGSGKTEVYLEAVAAALTAGRQALVL
LPEIALTAEFLTRVQERFGARPAEWHSGATMTERRRIFRMVGQGEAQLVIGARSALFLPYQNLGLIVVDEEHDTSYKQEE
GVLYNARDMAVLRASICGAQVVLASATPSLESWANAQAGKYAKLELTSRFGAAVMPQMRAIDMRVESLPSNRWVSDVLHK
EVEARMARGEQSMLFINRRGYAPITLCRACGHQIGCDHCDARMVEHRFLKRLVCHQCGESKPMPTVCPSCNAEDRLAAVG
PGVERLAEEATELFPDARIAALSSDMYGSARALKAEIEDIAAGGADIIIGTQLVAKGHNFPNLTLVGVIDADLGLQGSDL
RAAERTFQLMRQVAGRAGRAEKAGVALLQSYQPEHPVIRAILAGDEDEFWAAEARERKAAGVPPYGRMAGIILSGSDVAM
VFDAGNQLARQDAPLRAIGAQVFGPAPAPIARVRGRHRVRMLVKAEKSAPLQEALARWVAQLRIKGDLRVAVDIDPQSFY

Sequences:

>Translated_720_residues
MIAVLTTQPLGRTLDYRAPEGGCHLGAFVEVPLGPRKVLGVVWGPGQGDYERSKIRQAYRVLDAAPMREEMCLFLKRAAE
YTLTPLPAMLRLATRAPGLGDPPSMRKIYRRGTGEPDKMTAARRKVLAALAEYGDLAFTLKELAEMADVGTSVVKGLVAQ
GAVVEEDSPRDLPFPRLDPSRPGKALTADQAAGAAQLTQAVRSERYGTTLLRGVTGSGKTEVYLEAVAAALTAGRQALVL
LPEIALTAEFLTRVQERFGARPAEWHSGATMTERRRIFRMVGQGEAQLVIGARSALFLPYQNLGLIVVDEEHDTSYKQEE
GVLYNARDMAVLRASICGAQVVLASATPSLESWANAQAGKYAKLELTSRFGAAVMPQMRAIDMRVESLPSNRWVSDVLHK
EVEARMARGEQSMLFINRRGYAPITLCRACGHQIGCDHCDARMVEHRFLKRLVCHQCGESKPMPTVCPSCNAEDRLAAVG
PGVERLAEEATELFPDARIAALSSDMYGSARALKAEIEDIAAGGADIIIGTQLVAKGHNFPNLTLVGVIDADLGLQGSDL
RAAERTFQLMRQVAGRAGRAEKAGVALLQSYQPEHPVIRAILAGDEDEFWAAEARERKAAGVPPYGRMAGIILSGSDVAM
VFDAGNQLARQDAPLRAIGAQVFGPAPAPIARVRGRHRVRMLVKAEKSAPLQEALARWVAQLRIKGDLRVAVDIDPQSFY
>Mature_720_residues
MIAVLTTQPLGRTLDYRAPEGGCHLGAFVEVPLGPRKVLGVVWGPGQGDYERSKIRQAYRVLDAAPMREEMCLFLKRAAE
YTLTPLPAMLRLATRAPGLGDPPSMRKIYRRGTGEPDKMTAARRKVLAALAEYGDLAFTLKELAEMADVGTSVVKGLVAQ
GAVVEEDSPRDLPFPRLDPSRPGKALTADQAAGAAQLTQAVRSERYGTTLLRGVTGSGKTEVYLEAVAAALTAGRQALVL
LPEIALTAEFLTRVQERFGARPAEWHSGATMTERRRIFRMVGQGEAQLVIGARSALFLPYQNLGLIVVDEEHDTSYKQEE
GVLYNARDMAVLRASICGAQVVLASATPSLESWANAQAGKYAKLELTSRFGAAVMPQMRAIDMRVESLPSNRWVSDVLHK
EVEARMARGEQSMLFINRRGYAPITLCRACGHQIGCDHCDARMVEHRFLKRLVCHQCGESKPMPTVCPSCNAEDRLAAVG
PGVERLAEEATELFPDARIAALSSDMYGSARALKAEIEDIAAGGADIIIGTQLVAKGHNFPNLTLVGVIDADLGLQGSDL
RAAERTFQLMRQVAGRAGRAEKAGVALLQSYQPEHPVIRAILAGDEDEFWAAEARERKAAGVPPYGRMAGIILSGSDVAM
VFDAGNQLARQDAPLRAIGAQVFGPAPAPIARVRGRHRVRMLVKAEKSAPLQEALARWVAQLRIKGDLRVAVDIDPQSFY

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=739, Percent_Identity=34.7767253044655, Blast_Score=362, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 78011; Mature: 78011

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAVLTTQPLGRTLDYRAPEGGCHLGAFVEVPLGPRKVLGVVWGPGQGDYERSKIRQAYR
CEEEEECCCCCCCCCCCCCCCCCEECEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHH
VLDAAPMREEMCLFLKRAAEYTLTPLPAMLRLATRAPGLGDPPSMRKIYRRGTGEPDKMT
HHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHH
AARRKVLAALAEYGDLAFTLKELAEMADVGTSVVKGLVAQGAVVEEDSPRDLPFPRLDPS
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCC
RPGKALTADQAAGAAQLTQAVRSERYGTTLLRGVTGSGKTEVYLEAVAAALTAGRQALVL
CCCCCCCCHHHCCHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEE
LPEIALTAEFLTRVQERFGARPAEWHSGATMTERRRIFRMVGQGEAQLVIGARSALFLPY
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCEEEEEE
QNLGLIVVDEEHDTSYKQEEGVLYNARDMAVLRASICGAQVVLASATPSLESWANAQAGK
CCCCEEEEECCCCCCCHHHCCCEECCHHHHHHHHHHCCCEEEEECCCCCHHHHCCCCCCC
YAKLELTSRFGAAVMPQMRAIDMRVESLPSNRWVSDVLHKEVEARMARGEQSMLFINRRG
EEEEEEHHHCCHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCC
YAPITLCRACGHQIGCDHCDARMVEHRFLKRLVCHQCGESKPMPTVCPSCNAEDRLAAVG
CCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCC
PGVERLAEEATELFPDARIAALSSDMYGSARALKAEIEDIAAGGADIIIGTQLVAKGHNF
CCHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCC
PNLTLVGVIDADLGLQGSDLRAAERTFQLMRQVAGRAGRAEKAGVALLQSYQPEHPVIRA
CCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHH
ILAGDEDEFWAAEARERKAAGVPPYGRMAGIILSGSDVAMVFDAGNQLARQDAPLRAIGA
HHCCCCCHHHHHHHHHCCCCCCCCCCHHEEEEECCCCEEEEEECCCHHHHCCCCHHHHHH
QVFGPAPAPIARVRGRHRVRMLVKAEKSAPLQEALARWVAQLRIKGDLRVAVDIDPQSFY
HHCCCCCCHHHHHCCCHHEEEEEECCCCCCHHHHHHHHHHHHEECCCEEEEEECCCCCCC
>Mature Secondary Structure
MIAVLTTQPLGRTLDYRAPEGGCHLGAFVEVPLGPRKVLGVVWGPGQGDYERSKIRQAYR
CEEEEECCCCCCCCCCCCCCCCCEECEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHH
VLDAAPMREEMCLFLKRAAEYTLTPLPAMLRLATRAPGLGDPPSMRKIYRRGTGEPDKMT
HHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHH
AARRKVLAALAEYGDLAFTLKELAEMADVGTSVVKGLVAQGAVVEEDSPRDLPFPRLDPS
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCC
RPGKALTADQAAGAAQLTQAVRSERYGTTLLRGVTGSGKTEVYLEAVAAALTAGRQALVL
CCCCCCCCHHHCCHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEE
LPEIALTAEFLTRVQERFGARPAEWHSGATMTERRRIFRMVGQGEAQLVIGARSALFLPY
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCEEEEEE
QNLGLIVVDEEHDTSYKQEEGVLYNARDMAVLRASICGAQVVLASATPSLESWANAQAGK
CCCCEEEEECCCCCCCHHHCCCEECCHHHHHHHHHHCCCEEEEECCCCCHHHHCCCCCCC
YAKLELTSRFGAAVMPQMRAIDMRVESLPSNRWVSDVLHKEVEARMARGEQSMLFINRRG
EEEEEEHHHCCHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCC
YAPITLCRACGHQIGCDHCDARMVEHRFLKRLVCHQCGESKPMPTVCPSCNAEDRLAAVG
CCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCC
PGVERLAEEATELFPDARIAALSSDMYGSARALKAEIEDIAAGGADIIIGTQLVAKGHNF
CCHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCC
PNLTLVGVIDADLGLQGSDLRAAERTFQLMRQVAGRAGRAEKAGVALLQSYQPEHPVIRA
CCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHH
ILAGDEDEFWAAEARERKAAGVPPYGRMAGIILSGSDVAMVFDAGNQLARQDAPLRAIGA
HHCCCCCHHHHHHHHHCCCCCCCCCCHHEEEEECCCCEEEEEECCCHHHHCCCCHHHHHH
QVFGPAPAPIARVRGRHRVRMLVKAEKSAPLQEALARWVAQLRIKGDLRVAVDIDPQSFY
HHCCCCCCHHHHHCCCHHEEEEEECCCCCCHHHHHHHHHHHHEECCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA