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The map label for this gene is proS

Identifier: 110679326

GI number: 110679326

Start: 1954002

End: 1955357

Strand: Direct

Name: proS

Synonym: RD1_2045

Alternate gene names: 110679326

Gene position: 1954002-1955357 (Clockwise)

Preceding gene: 110679321

Following gene: 110679327

Centisome position: 47.28

GC content: 57.67

Gene sequence:

>1356_bases
ATGCGCCTGACCCGATACTTTATGCCCGTACTGCGCGAAAACCCGGCAGAGGCGCAGATTGTCAGCCACCGTCTGATGTT
GCGGGCCGGGATGATCAAACAGAACGCGGCAGGGATTTATTCGTGGCTGCCGCTGGGGTTCAAGGTGCTGCGCAAGCTGG
AAAACATCGTGCATGAGGAACAAATCCGCGCAGGCCACGTGCCGATGCTGATGCCCACGATGCAATCGGCGGATCTATGG
CGCGAAAGTGGGCGCTATGATGCCTATGGGCCTGAAATGCTGCGGATCAGGGACCGACAGGACCGCGACATGCTCTTTGG
GCCGACCAATGAGGAAATGATCACCGACATTTTCCGTAGCCATGTGAGCAGCTACAAGGATTTGCCACTGACGCTCTATC
ACATCCAGTGGAAATTCCGCGACGAGATGCGCCCGCGCTTTGGTGTGATGCGGGGGCGCGAGTTCTTTATGAAAGACGGC
TACAATTTCGATCTGACGAAAGAGGACGCATTGCACGCTTATAACCGCCATCTGGTCAGCTATTTGCGCACCTATGAGCG
GATGGGATTGCAGGCCATTCCGATGCGCGCTGATTCCGGCCCGATTGGCGGCGATGACACGCATGAATTCCTGGTCTTGG
CGGACACCGGAGAATCCGAGGTGTTCTATGACAGCGCCGTGACCGATCTGACCTTTGGGGACCGTGACATTGACTATGAC
AGCGTCGAACAGTGTCAGGGGGTGATGGAAGAATTCACCAACCTTTACGCGCGCACGGATGAAACCCATGATCAGGCCCT
GTTCGCCGCGATCCCGGCTGAGCGTCAGCGCCAGGCGCGGGGGATCGAGGTCGGCCAGATCTTCTATTTTGGCACCAAGT
ATTCCGATGCGATGGGCGCTAAGGTGCAGGGCCCCGATGGCAAACCGACGGCTGTACATATGGGCAGCCACGGGATCGGG
GTCAGCCGCCTTGTCGGCGCGATCATCGAGGCCAGCCATGACGAGAAGGGCATCATCTGGCCCGAAGGCGTGACGCCGTT
CCACTGCGGAATCGTGAACCTGAAACAGGGCGATACGGAGGCTGATGCAGCCTGTGATGCGCTTTATGCGTCATTGCGTG
CGCTCGGGCTTGATCCGCTTTATGATGATCGCAAGGAACGCGCAGGCGGCAAATTCGCAACCATGGATCTGATCGGATTG
CCATGGCGGATCACGGTGGGGCCACGGGGGCTGAAAAACGGTGTTGTCGAACTGACCAGCCGCAAGACCGGCGAAAGCTC
AGAGCTTCCACCCGAAGAGGCAATTGAAAAGATTGCTAAAATATACGCGCCCCATCATGTGAGTGGCAACCTGTAA

Upstream 100 bases:

>100_bases
GACTGGTTTTCAGCTCAGCCTGTTGAGACGGTTTGGCGATTGCGAGCCGCGCGCCTTTGGCCTAATCAAGGGCATTCCGT
AGACCAGATGAGGACACCCC

Downstream 100 bases:

>100_bases
GCAAGGACGCGCGGCGCGGGGCTTTGAGCGGCGCGCTGTTTCTGCTAGGCTGTCGAGGCGCCATCGGGGGATGAGCGGGA
GAAGGCGATGATCCTTCGCG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MRLTRYFMPVLRENPAEAQIVSHRLMLRAGMIKQNAAGIYSWLPLGFKVLRKLENIVHEEQIRAGHVPMLMPTMQSADLW
RESGRYDAYGPEMLRIRDRQDRDMLFGPTNEEMITDIFRSHVSSYKDLPLTLYHIQWKFRDEMRPRFGVMRGREFFMKDG
YNFDLTKEDALHAYNRHLVSYLRTYERMGLQAIPMRADSGPIGGDDTHEFLVLADTGESEVFYDSAVTDLTFGDRDIDYD
SVEQCQGVMEEFTNLYARTDETHDQALFAAIPAERQRQARGIEVGQIFYFGTKYSDAMGAKVQGPDGKPTAVHMGSHGIG
VSRLVGAIIEASHDEKGIIWPEGVTPFHCGIVNLKQGDTEADAACDALYASLRALGLDPLYDDRKERAGGKFATMDLIGL
PWRITVGPRGLKNGVVELTSRKTGESSELPPEEAIEKIAKIYAPHHVSGNL

Sequences:

>Translated_451_residues
MRLTRYFMPVLRENPAEAQIVSHRLMLRAGMIKQNAAGIYSWLPLGFKVLRKLENIVHEEQIRAGHVPMLMPTMQSADLW
RESGRYDAYGPEMLRIRDRQDRDMLFGPTNEEMITDIFRSHVSSYKDLPLTLYHIQWKFRDEMRPRFGVMRGREFFMKDG
YNFDLTKEDALHAYNRHLVSYLRTYERMGLQAIPMRADSGPIGGDDTHEFLVLADTGESEVFYDSAVTDLTFGDRDIDYD
SVEQCQGVMEEFTNLYARTDETHDQALFAAIPAERQRQARGIEVGQIFYFGTKYSDAMGAKVQGPDGKPTAVHMGSHGIG
VSRLVGAIIEASHDEKGIIWPEGVTPFHCGIVNLKQGDTEADAACDALYASLRALGLDPLYDDRKERAGGKFATMDLIGL
PWRITVGPRGLKNGVVELTSRKTGESSELPPEEAIEKIAKIYAPHHVSGNL
>Mature_451_residues
MRLTRYFMPVLRENPAEAQIVSHRLMLRAGMIKQNAAGIYSWLPLGFKVLRKLENIVHEEQIRAGHVPMLMPTMQSADLW
RESGRYDAYGPEMLRIRDRQDRDMLFGPTNEEMITDIFRSHVSSYKDLPLTLYHIQWKFRDEMRPRFGVMRGREFFMKDG
YNFDLTKEDALHAYNRHLVSYLRTYERMGLQAIPMRADSGPIGGDDTHEFLVLADTGESEVFYDSAVTDLTFGDRDIDYD
SVEQCQGVMEEFTNLYARTDETHDQALFAAIPAERQRQARGIEVGQIFYFGTKYSDAMGAKVQGPDGKPTAVHMGSHGIG
VSRLVGAIIEASHDEKGIIWPEGVTPFHCGIVNLKQGDTEADAACDALYASLRALGLDPLYDDRKERAGGKFATMDLIGL
PWRITVGPRGLKNGVVELTSRKTGESSELPPEEAIEKIAKIYAPHHVSGNL

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=418, Percent_Identity=33.2535885167464, Blast_Score=236, Evalue=4e-62,
Organism=Escherichia coli, GI1786392, Length=223, Percent_Identity=52.914798206278, Blast_Score=264, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI115532348, Length=404, Percent_Identity=32.6732673267327, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI193203271, Length=94, Percent_Identity=36.1702127659575, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320931, Length=239, Percent_Identity=29.7071129707113, Blast_Score=146, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24656200, Length=455, Percent_Identity=28.3516483516484, Blast_Score=199, Evalue=4e-51,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_ROSDO (Q168E6)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_682333.1
- ProteinModelPortal:   Q168E6
- SMR:   Q168E6
- STRING:   Q168E6
- GeneID:   4196672
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_2045
- NMPDR:   fig|375451.6.peg.1910
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   Q168E6
- ProtClustDB:   PRK12325
- BioCyc:   RDEN375451:RD1_2045-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01570
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.15

Molecular weight: Translated: 50943; Mature: 50943

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLTRYFMPVLRENPAEAQIVSHRLMLRAGMIKQNAAGIYSWLPLGFKVLRKLENIVHEE
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
QIRAGHVPMLMPTMQSADLWRESGRYDAYGPEMLRIRDRQDRDMLFGPTNEEMITDIFRS
HHHCCCCCEEECCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCEECCCCHHHHHHHHHH
HVSSYKDLPLTLYHIQWKFRDEMRPRFGVMRGREFFMKDGYNFDLTKEDALHAYNRHLVS
HHHHCCCCCEEEEEEEEEECHHCCHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHH
YLRTYERMGLQAIPMRADSGPIGGDDTHEFLVLADTGESEVFYDSAVTDLTFGDRDIDYD
HHHHHHHCCCEEECEECCCCCCCCCCCCEEEEEEECCCCCEEECCCHHCCCCCCCCCCHH
SVEQCQGVMEEFTNLYARTDETHDQALFAAIPAERQRQARGIEVGQIFYFGTKYSDAMGA
HHHHHHHHHHHHHHHHHCCCCCHHCEEEEECCHHHHHHHCCCEEEEEEEECCCCCCCCCC
KVQGPDGKPTAVHMGSHGIGVSRLVGAIIEASHDEKGIIWPEGVTPFHCGIVNLKQGDTE
EEECCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCH
ADAACDALYASLRALGLDPLYDDRKERAGGKFATMDLIGLPWRITVGPRGLKNGVVELTS
HHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEEEECCCEEEEECCCCHHCCCEEEEC
RKTGESSELPPEEAIEKIAKIYAPHHVSGNL
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRLTRYFMPVLRENPAEAQIVSHRLMLRAGMIKQNAAGIYSWLPLGFKVLRKLENIVHEE
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
QIRAGHVPMLMPTMQSADLWRESGRYDAYGPEMLRIRDRQDRDMLFGPTNEEMITDIFRS
HHHCCCCCEEECCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCEECCCCHHHHHHHHHH
HVSSYKDLPLTLYHIQWKFRDEMRPRFGVMRGREFFMKDGYNFDLTKEDALHAYNRHLVS
HHHHCCCCCEEEEEEEEEECHHCCHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHH
YLRTYERMGLQAIPMRADSGPIGGDDTHEFLVLADTGESEVFYDSAVTDLTFGDRDIDYD
HHHHHHHCCCEEECEECCCCCCCCCCCCEEEEEEECCCCCEEECCCHHCCCCCCCCCCHH
SVEQCQGVMEEFTNLYARTDETHDQALFAAIPAERQRQARGIEVGQIFYFGTKYSDAMGA
HHHHHHHHHHHHHHHHHCCCCCHHCEEEEECCHHHHHHHCCCEEEEEEEECCCCCCCCCC
KVQGPDGKPTAVHMGSHGIGVSRLVGAIIEASHDEKGIIWPEGVTPFHCGIVNLKQGDTE
EEECCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCH
ADAACDALYASLRALGLDPLYDDRKERAGGKFATMDLIGLPWRITVGPRGLKNGVVELTS
HHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEEEECCCEEEEECCCCHHCCCEEEEC
RKTGESSELPPEEAIEKIAKIYAPHHVSGNL
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA