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The map label for this gene is bcr [H]

Identifier: 110679309

GI number: 110679309

Start: 1935291

End: 1936556

Strand: Reverse

Name: bcr [H]

Synonym: RD1_2027

Alternate gene names: 110679309

Gene position: 1936556-1935291 (Counterclockwise)

Preceding gene: 110679315

Following gene: 110679308

Centisome position: 46.85

GC content: 60.19

Gene sequence:

>1266_bases
ATGGACAAGCCGCCAGTGCTGACCTACAAAACCGTTATGTCTAAACTACCCGCCGTTCGTTTCTTTGATCGGACTACACC
CCCGCACATTTCGACGCTGATCCTGCTGGCAGGCATGTCCGCGATTGTCATGAACATGTTCCTGCCAAGTCTGCCGCGCA
TGACCGCGCATTTTGAAACGGATTACGGGATCATGCAGCTTTCGGTGGCGCTCTATCTCGGCATCAGCGGGGTGTTGCAG
CTGTTTCTGGGGCCGATCTCGGATAACTTCGGGCGCCGGCCGGTCATTCTGGTCGGTCTGGCCGGCTTTCTTCTCGCCAC
GCTGGGCTGCATTTATGCGCCATCGGTCGAGGTGTTCCTGTTCTTTCGCATGTGTCAGGCCGTGGTCGCTGTGGCGATGG
TGCTGAGCCGTGCGGCCGTGCGCGACATGTTCGAACAGGACAAGGCAGCCTCCATGATCGGCTATGTCACGATGGGCATG
GCGGTGGTGCCCATGGTCAGCCCGATGTTTGGCGGAATTCTGGATCAGATGTTTGGATGGGAGGCTAATTTCTGGGCCCT
TTTCATTCTCGGTGCGCTCATGCTCTGGGTGGCATGGGCGGATATGGGAGAAACGGCCGTCAAGAGCGGCAAGACCCTGA
CCGCGCAATTCGCCGAATACCCCGAACTCCTCACCTCCCCGCGGTTCTGGGGCTATTCGCTGGCGGCGGGTTTTTGTTCA
GGCGCGTTTTTCGCCTATCTGGGCGGCGGGCCTTTCGTGGGCTCCGTGGTCTTCGGCATGACACCCTTCTGGGTCGGGAT
TTTCTTTGGCGCGCCTGCAATCGGGTATTTCTTTGGCAATATGTTCACCGGAATGTTCGCGCAACGCGTCGGGATCAACA
CGCTGATCCTGATCGGTGCCTTGATCAATGCCGCGGGCGTGGCGCTGTCTTTGCTAATCTTCCTCCTCGGCGCGGGCAGC
CCGATCAGCTTTTTTGGCCTGATGACATTCGTCGGCTTGGGCAACGGCCTTGTGATCCCGAATGCGACCGCCGGCATGCT
GTCGGTGCGCCCGCATCTGGCAGGCACGGCCTCCGGCCTTGGCGGGGCCATCATGATCGGCGGCGGTGCGGCATTGAGCG
CACTGGCAGGCGCGACCCTGAACGAAGCCACAGGCGCATTTCCGCTGCTTTACATCATGCTTGCGACGGCCCTCGCGGCA
GTTGCGTCGATCATGGCAGTCTATGCACGCGAACGCCGCCTGACGCGCCAGAACAGCCCGTTTTAA

Upstream 100 bases:

>100_bases
CCTCTGAACACTTCGGGGTGCGGGGTCCTTTCGCAGCTCATCAAAACCCGGCCCGGGACGGTGTGTCTGCGCGCCCGGGC
GACGGATTACGGGTCTGTCG

Downstream 100 bases:

>100_bases
GCCACCGGTTAGGCAAGTCTTAACCATGATGACGCACCTTGCTTCGGCGGGTTTGCAAAGTTAGCCTTTGCCGACTCGAC
CCATTTGCAAAATCTGCGGA

Product: MFS protein, putative

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MDKPPVLTYKTVMSKLPAVRFFDRTTPPHISTLILLAGMSAIVMNMFLPSLPRMTAHFETDYGIMQLSVALYLGISGVLQ
LFLGPISDNFGRRPVILVGLAGFLLATLGCIYAPSVEVFLFFRMCQAVVAVAMVLSRAAVRDMFEQDKAASMIGYVTMGM
AVVPMVSPMFGGILDQMFGWEANFWALFILGALMLWVAWADMGETAVKSGKTLTAQFAEYPELLTSPRFWGYSLAAGFCS
GAFFAYLGGGPFVGSVVFGMTPFWVGIFFGAPAIGYFFGNMFTGMFAQRVGINTLILIGALINAAGVALSLLIFLLGAGS
PISFFGLMTFVGLGNGLVIPNATAGMLSVRPHLAGTASGLGGAIMIGGGAALSALAGATLNEATGAFPLLYIMLATALAA
VASIMAVYARERRLTRQNSPF

Sequences:

>Translated_421_residues
MDKPPVLTYKTVMSKLPAVRFFDRTTPPHISTLILLAGMSAIVMNMFLPSLPRMTAHFETDYGIMQLSVALYLGISGVLQ
LFLGPISDNFGRRPVILVGLAGFLLATLGCIYAPSVEVFLFFRMCQAVVAVAMVLSRAAVRDMFEQDKAASMIGYVTMGM
AVVPMVSPMFGGILDQMFGWEANFWALFILGALMLWVAWADMGETAVKSGKTLTAQFAEYPELLTSPRFWGYSLAAGFCS
GAFFAYLGGGPFVGSVVFGMTPFWVGIFFGAPAIGYFFGNMFTGMFAQRVGINTLILIGALINAAGVALSLLIFLLGAGS
PISFFGLMTFVGLGNGLVIPNATAGMLSVRPHLAGTASGLGGAIMIGGGAALSALAGATLNEATGAFPLLYIMLATALAA
VASIMAVYARERRLTRQNSPF
>Mature_421_residues
MDKPPVLTYKTVMSKLPAVRFFDRTTPPHISTLILLAGMSAIVMNMFLPSLPRMTAHFETDYGIMQLSVALYLGISGVLQ
LFLGPISDNFGRRPVILVGLAGFLLATLGCIYAPSVEVFLFFRMCQAVVAVAMVLSRAAVRDMFEQDKAASMIGYVTMGM
AVVPMVSPMFGGILDQMFGWEANFWALFILGALMLWVAWADMGETAVKSGKTLTAQFAEYPELLTSPRFWGYSLAAGFCS
GAFFAYLGGGPFVGSVVFGMTPFWVGIFFGAPAIGYFFGNMFTGMFAQRVGINTLILIGALINAAGVALSLLIFLLGAGS
PISFFGLMTFVGLGNGLVIPNATAGMLSVRPHLAGTASGLGGAIMIGGGAALSALAGATLNEATGAFPLLYIMLATALAA
VASIMAVYARERRLTRQNSPF

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=402, Percent_Identity=28.8557213930348, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI48994889, Length=343, Percent_Identity=26.8221574344023, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI87082312, Length=346, Percent_Identity=31.5028901734104, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787065, Length=307, Percent_Identity=26.3843648208469, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1790146, Length=163, Percent_Identity=26.9938650306748, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790794, Length=179, Percent_Identity=27.3743016759777, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324264, Length=145, Percent_Identity=26.8965517241379, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6319517, Length=174, Percent_Identity=25.2873563218391, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44604; Mature: 44604

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
6.2 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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C
>Mature Secondary Structure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C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]