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The map label for this gene is amiD [H]

Identifier: 110679281

GI number: 110679281

Start: 1906800

End: 1907474

Strand: Reverse

Name: amiD [H]

Synonym: RD1_1998

Alternate gene names: 110679281

Gene position: 1907474-1906800 (Counterclockwise)

Preceding gene: 110679282

Following gene: 110679280

Centisome position: 46.15

GC content: 63.7

Gene sequence:

>675_bases
TTGGGATCCGACCCCGTTTCGCAGTGCTGCGCAAGGATAGCCGTGCGCCACTGCCCTTCGCTGAATTTTGGCCCACGGCG
CAATAGCCTGACGCCCATACTCATCGTGATACACTACACGGCCATGGCCAGCGCCGAGGCAGCGATCGCGCGCCTTTGTG
ATCCGCAGGCAGAGGTTTCCGCACATTATCTGATTGATCGCAGCGGCCCCGTGACACAAATGGTCAATGAAGAGCACCGC
GCCTGGCACGCGGGCGTCGGGGAATGGCGCGGGCTGACTGACATCAATTCCCGTTCCATCGGAATCGAACTGGATAACGA
CGGCACGCATCCGTTTCCCGAACCACAGATGGCCGCGCTGGAAGACCTGTTGCGGGGTATCCGGTCCCGCTGGCCCGTGG
CGGCAAGCGATATCATCGGCCATTCAGACATGGCGCCGGGGCGCAAATCGGACCCCGGGCCACGCTTTGACTGGGCGCGG
CTGGCCCGACAGGGGTTGGCAGCACCGGTGAGCCCGCAGATGCAAGGTGAGGGGTTTGAGCAATCCGCACAGCGCGCAGG
GTTCACCGCCCCTGTGGATCGCGCAACGCTGTTGACCGCCACGCGGCTGCGCTTTGCGCCCTGGCGCGAGGGTCCGATCA
CGGATGACGATCTGCAGCTTTACCACCGGATTTGA

Upstream 100 bases:

>100_bases
ATCCGTCTCCCGACCGGGCGCAACTGGATTTTCTGGCGCGCGGTGGTCCCAAACGCGACCGCAAGGGTCTTGACCCGGAT
GGGGATGGCTATGCCTGCAG

Downstream 100 bases:

>100_bases
CCGCGCGCCCGCGCAATGGCCATTGACGCTGTTGCGATTTGAGCGCATCACGCACCCGCGCGGAGGGCCGGATGGCCGCG
GCACTGGCAACAGGGTCGAG

Product: N-acetylmuramoyl-L-alanine amidase, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MGSDPVSQCCARIAVRHCPSLNFGPRRNSLTPILIVIHYTAMASAEAAIARLCDPQAEVSAHYLIDRSGPVTQMVNEEHR
AWHAGVGEWRGLTDINSRSIGIELDNDGTHPFPEPQMAALEDLLRGIRSRWPVAASDIIGHSDMAPGRKSDPGPRFDWAR
LARQGLAAPVSPQMQGEGFEQSAQRAGFTAPVDRATLLTATRLRFAPWREGPITDDDLQLYHRI

Sequences:

>Translated_224_residues
MGSDPVSQCCARIAVRHCPSLNFGPRRNSLTPILIVIHYTAMASAEAAIARLCDPQAEVSAHYLIDRSGPVTQMVNEEHR
AWHAGVGEWRGLTDINSRSIGIELDNDGTHPFPEPQMAALEDLLRGIRSRWPVAASDIIGHSDMAPGRKSDPGPRFDWAR
LARQGLAAPVSPQMQGEGFEQSAQRAGFTAPVDRATLLTATRLRFAPWREGPITDDDLQLYHRI
>Mature_223_residues
GSDPVSQCCARIAVRHCPSLNFGPRRNSLTPILIVIHYTAMASAEAAIARLCDPQAEVSAHYLIDRSGPVTQMVNEEHRA
WHAGVGEWRGLTDINSRSIGIELDNDGTHPFPEPQMAALEDLLRGIRSRWPVAASDIIGHSDMAPGRKSDPGPRFDWARL
ARQGLAAPVSPQMQGEGFEQSAQRAGFTAPVDRATLLTATRLRFAPWREGPITDDDLQLYHRI

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=181, Percent_Identity=41.4364640883978, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1786300, Length=166, Percent_Identity=37.3493975903614, Blast_Score=107, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 24635; Mature: 24504

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSDPVSQCCARIAVRHCPSLNFGPRRNSLTPILIVIHYTAMASAEAAIARLCDPQAEVS
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCHHHCC
AHYLIDRSGPVTQMVNEEHRAWHAGVGEWRGLTDINSRSIGIELDNDGTHPFPEPQMAAL
EEEEEECCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHH
EDLLRGIRSRWPVAASDIIGHSDMAPGRKSDPGPRFDWARLARQGLAAPVSPQMQGEGFE
HHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHH
QSAQRAGFTAPVDRATLLTATRLRFAPWREGPITDDDLQLYHRI
HHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure 
GSDPVSQCCARIAVRHCPSLNFGPRRNSLTPILIVIHYTAMASAEAAIARLCDPQAEVS
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCHHHCC
AHYLIDRSGPVTQMVNEEHRAWHAGVGEWRGLTDINSRSIGIELDNDGTHPFPEPQMAAL
EEEEEECCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHH
EDLLRGIRSRWPVAASDIIGHSDMAPGRKSDPGPRFDWARLARQGLAAPVSPQMQGEGFE
HHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHH
QSAQRAGFTAPVDRATLLTATRLRFAPWREGPITDDDLQLYHRI
HHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]