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The map label for this gene is purD [H]

Identifier: 110679216

GI number: 110679216

Start: 1838325

End: 1839590

Strand: Reverse

Name: purD [H]

Synonym: RD1_1927

Alternate gene names: 110679216

Gene position: 1839590-1838325 (Counterclockwise)

Preceding gene: 110679218

Following gene: 110679213

Centisome position: 44.51

GC content: 63.27

Gene sequence:

>1266_bases
ATGAACATTCTTATTCTCGGCAGCGGGGGGCGCGAACACGCTCTGGCCTGGGCGGTCAAACAGAACCCCAAATGTGATCG
CCTGATCATGGCCCCCGGCAATGCGGGGATTGCGGGGATCGCCGAATGCGCGTCCCTCAACATCGAGGATGGCGGGGCCG
TCGCAGGTTTTGCCGATGCAAATGCCATTGATCTGGTGATCGTCGGGCCCGAAGCGCCCCTCGCCGCGGGTGTCGCGGAT
CGCCTGCGGGATGCGGGCATTTCGGTTTTCGGCCCCGGCCTCGAAGCAGCAAAGCTGGAGGCCTCGAAAGCCTTCACCAA
AACCATCTGCGATGCAGCGGGTGCGCCGACCGCCGCTTATGCGCATTTCACCGACCGGGCTGCGGCGCGGGCCTATGTCA
AGGCACAAGGCGCACCCATTGTCATCAAGGCCGACGGTCTGGCCGCGGGCAAAGGCGTGATCATCGCCCAGACGGTCGAC
GAGGCGCTGAACGCTCTTGATGACATGTTCGGCGGCAGTTTCGGCGCGGCGGGCGCAGAAGTCGTGATCGAAGAATTCAT
GGAAGGCGAAGAGGCGTCCTTCTTTGTGCTCTGCGATGGCGAAACCGTGCTGCCCATCGGCACGGCGCAGGATCACAAGC
GTGTGGGTGACGGCGACACAGGCCCCAATACCGGCGGCATGGGCGCCTATTCCCCTGCCCCGGTGATGACGCCCGAGGTA
ACGCAGGCGGCGCTGGACCAGATCGTGCAGCCAACCATGGCCGAAATGACCCGGCGCGGGACGCCCTATCAAGGGGTGCT
CTTCGTCGGCCTGATGATCAAGGACGGCGCACCCCGGCTCGTCGAATACAACGTGCGCTTTGGCGACCCGGAATGTCAGG
CTCTGATGATGCGCCTTGGTGCGCAGGTATTGGACCTTATTCAGGCCACCGCCGAAGGCAGACTGGGAGAGGTTCAGGTC
AATTGGGCAGATGATCATGCTTTGACGGTCGTGCTGGCCGCGCGCGGATATCCCGGCAGCTATGAAAAAGGCTCCGTCAT
TGGCGGGCTGGAAGGTTTCAAGGAAGACAGCAAACAGATGATCTTTCATGCCGGGACAGCCCGCAGGGACGGTCGCTACA
TCGCCAGCGGTGGTCGGGTTCTTGGTGTGACCGCGCGCGCCGCATCCCTGAGCGAGGCGCGCGAAAGGGCCTACGCCATG
GTGGATCAGATCGACTGGCCGGACGGCTTTTGTCGCCGGGACATCGGCTGGCGCGCATTGGATTAG

Upstream 100 bases:

>100_bases
GGGATCGTCAAGCAAGTCCATAGCGCCTCGGGTGTTGTCACAGGTTCCCCGCCAGCTTAGAACGCCCTGAACCTAAGGCC
AAGCAGATCGGGAGCCCTTC

Downstream 100 bases:

>100_bases
AGCGTCTGACGAAATACCTGAAACATCACGTTTCACATACCGCGTTGAAACCGTCGATTTCAGGCAGCGCTTGCGTGGTT
CAGGTTTTTCGCATGACGCT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MNILILGSGGREHALAWAVKQNPKCDRLIMAPGNAGIAGIAECASLNIEDGGAVAGFADANAIDLVIVGPEAPLAAGVAD
RLRDAGISVFGPGLEAAKLEASKAFTKTICDAAGAPTAAYAHFTDRAAARAYVKAQGAPIVIKADGLAAGKGVIIAQTVD
EALNALDDMFGGSFGAAGAEVVIEEFMEGEEASFFVLCDGETVLPIGTAQDHKRVGDGDTGPNTGGMGAYSPAPVMTPEV
TQAALDQIVQPTMAEMTRRGTPYQGVLFVGLMIKDGAPRLVEYNVRFGDPECQALMMRLGAQVLDLIQATAEGRLGEVQV
NWADDHALTVVLAARGYPGSYEKGSVIGGLEGFKEDSKQMIFHAGTARRDGRYIASGGRVLGVTARAASLSEARERAYAM
VDQIDWPDGFCRRDIGWRALD

Sequences:

>Translated_421_residues
MNILILGSGGREHALAWAVKQNPKCDRLIMAPGNAGIAGIAECASLNIEDGGAVAGFADANAIDLVIVGPEAPLAAGVAD
RLRDAGISVFGPGLEAAKLEASKAFTKTICDAAGAPTAAYAHFTDRAAARAYVKAQGAPIVIKADGLAAGKGVIIAQTVD
EALNALDDMFGGSFGAAGAEVVIEEFMEGEEASFFVLCDGETVLPIGTAQDHKRVGDGDTGPNTGGMGAYSPAPVMTPEV
TQAALDQIVQPTMAEMTRRGTPYQGVLFVGLMIKDGAPRLVEYNVRFGDPECQALMMRLGAQVLDLIQATAEGRLGEVQV
NWADDHALTVVLAARGYPGSYEKGSVIGGLEGFKEDSKQMIFHAGTARRDGRYIASGGRVLGVTARAASLSEARERAYAM
VDQIDWPDGFCRRDIGWRALD
>Mature_421_residues
MNILILGSGGREHALAWAVKQNPKCDRLIMAPGNAGIAGIAECASLNIEDGGAVAGFADANAIDLVIVGPEAPLAAGVAD
RLRDAGISVFGPGLEAAKLEASKAFTKTICDAAGAPTAAYAHFTDRAAARAYVKAQGAPIVIKADGLAAGKGVIIAQTVD
EALNALDDMFGGSFGAAGAEVVIEEFMEGEEASFFVLCDGETVLPIGTAQDHKRVGDGDTGPNTGGMGAYSPAPVMTPEV
TQAALDQIVQPTMAEMTRRGTPYQGVLFVGLMIKDGAPRLVEYNVRFGDPECQALMMRLGAQVLDLIQATAEGRLGEVQV
NWADDHALTVVLAARGYPGSYEKGSVIGGLEGFKEDSKQMIFHAGTARRDGRYIASGGRVLGVTARAASLSEARERAYAM
VDQIDWPDGFCRRDIGWRALD

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=423, Percent_Identity=44.6808510638298, Blast_Score=359, Evalue=3e-99,
Organism=Homo sapiens, GI209869995, Length=423, Percent_Identity=44.6808510638298, Blast_Score=359, Evalue=3e-99,
Organism=Homo sapiens, GI209869993, Length=423, Percent_Identity=44.6808510638298, Blast_Score=359, Evalue=3e-99,
Organism=Homo sapiens, GI28416899, Length=423, Percent_Identity=44.6808510638298, Blast_Score=358, Evalue=4e-99,
Organism=Escherichia coli, GI1790438, Length=426, Percent_Identity=51.8779342723005, Blast_Score=427, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17567511, Length=421, Percent_Identity=40.855106888361, Blast_Score=297, Evalue=7e-81,
Organism=Saccharomyces cerevisiae, GI6321203, Length=441, Percent_Identity=43.7641723356009, Blast_Score=322, Evalue=8e-89,
Organism=Drosophila melanogaster, GI24582400, Length=413, Percent_Identity=40.4358353510896, Blast_Score=294, Evalue=9e-80,
Organism=Drosophila melanogaster, GI62471619, Length=413, Percent_Identity=40.4358353510896, Blast_Score=294, Evalue=1e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 43915; Mature: 43915

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILILGSGGREHALAWAVKQNPKCDRLIMAPGNAGIAGIAECASLNIEDGGAVAGFADA
CEEEEECCCCCCCEEEEEECCCCCCCEEEECCCCCCCHHHHHHHCCCCCCCCEEEEECCC
NAIDLVIVGPEAPLAAGVADRLRDAGISVFGPGLEAAKLEASKAFTKTICDAAGAPTAAY
CEEEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEE
AHFTDRAAARAYVKAQGAPIVIKADGLAAGKGVIIAQTVDEALNALDDMFGGSFGAAGAE
HHHHHHHHHHHHEEECCCEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCHHH
VVIEEFMEGEEASFFVLCDGETVLPIGTAQDHKRVGDGDTGPNTGGMGAYSPAPVMTPEV
HHHHHHHCCCCCEEEEEECCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHH
TQAALDQIVQPTMAEMTRRGTPYQGVLFVGLMIKDGAPRLVEYNVRFGDPECQALMMRLG
HHHHHHHHHCHHHHHHHHCCCCCCCEEEEEEEEECCCCCEEEEEEEECCHHHHHHHHHHH
AQVLDLIQATAEGRLGEVQVNWADDHALTVVLAARGYPGSYEKGSVIGGLEGFKEDSKQM
HHHHHHHHHHHCCCEEEEEEEECCCCEEEEEEEECCCCCCCCCCCEEECHHHHHCCCCEE
IFHAGTARRDGRYIASGGRVLGVTARAASLSEARERAYAMVDQIDWPDGFCRRDIGWRAL
EEECCCCCCCCEEEECCCEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCEEC
D
C
>Mature Secondary Structure
MNILILGSGGREHALAWAVKQNPKCDRLIMAPGNAGIAGIAECASLNIEDGGAVAGFADA
CEEEEECCCCCCCEEEEEECCCCCCCEEEECCCCCCCHHHHHHHCCCCCCCCEEEEECCC
NAIDLVIVGPEAPLAAGVADRLRDAGISVFGPGLEAAKLEASKAFTKTICDAAGAPTAAY
CEEEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEE
AHFTDRAAARAYVKAQGAPIVIKADGLAAGKGVIIAQTVDEALNALDDMFGGSFGAAGAE
HHHHHHHHHHHHEEECCCEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCHHH
VVIEEFMEGEEASFFVLCDGETVLPIGTAQDHKRVGDGDTGPNTGGMGAYSPAPVMTPEV
HHHHHHHCCCCCEEEEEECCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHH
TQAALDQIVQPTMAEMTRRGTPYQGVLFVGLMIKDGAPRLVEYNVRFGDPECQALMMRLG
HHHHHHHHHCHHHHHHHHCCCCCCCEEEEEEEEECCCCCEEEEEEEECCHHHHHHHHHHH
AQVLDLIQATAEGRLGEVQVNWADDHALTVVLAARGYPGSYEKGSVIGGLEGFKEDSKQM
HHHHHHHHHHHCCCEEEEEEEECCCCEEEEEEEECCCCCCCCCCCEEECHHHHHCCCCEE
IFHAGTARRDGRYIASGGRVLGVTARAASLSEARERAYAMVDQIDWPDGFCRRDIGWRAL
EEECCCCCCCCEEEECCCEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCEEC
D
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]