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The map label for this gene is purA [H]

Identifier: 110679201

GI number: 110679201

Start: 1823341

End: 1824705

Strand: Direct

Name: purA [H]

Synonym: RD1_1912

Alternate gene names: 110679201

Gene position: 1823341-1824705 (Clockwise)

Preceding gene: 110679199

Following gene: 110679202

Centisome position: 44.12

GC content: 60.29

Gene sequence:

>1365_bases
TTGCGGTTTCGCCCCGGCGCAGGCCTCGCTATACGGGCGCGGGTTTTATCCGAAAACAAAGGACCAGATATGGCAAACGT
CGTCGTTGTGGGCGCTCAGTGGGGCGACGAAGGAAAAGGCAAGATCGTCGACTGGCTGTCTGAACGCGCCGATGTGATCG
CGCGCTTTCAGGGCGGTCACAATGCCGGGCATACGCTGGTCATTGATGGCAAGGTCTACAAGCTGAACGCGTTGCCCTCC
GGTGTGGTGCGGGGCGGCAAACTGTCTGTTATTGGCAACGGCGTTGTGCTGGACCCTTGGCACCTGATCAAGGAAATCGA
TATTATCCGCGGGCAGGGCGTCGATATTTCTCCCGAGACCTTGATGATCGCCGAAAACACACCGCTGATCCTGCCCATTC
ATGGCGAATTGGACCGTGCGCGCGAGGAAGCCGCGAGCAAAGGCACCAAGATCGGTACGACGGGCCGGGGTATTGGCCCC
GCCTATGAGGATAAGGTCGGTCGCCGTTCGGTCCGGGTCGCGGATCTGGCCGATACTGCCACGCTCGAAGCGCGGGTGGA
TCGTGCGCTGCAGCACCACGATCCGCTGCGCAAAGGCCTCGGGATTGAGCCGGTGGACCGGGACGCGCTGGTGGCGCAAC
TGGTCGAAATTGCACCGCACATCCTCAAGTACGCAGCGCCCGTGTGGAAGGTGCTCAACGAAAAGCGGCGCGCGGGCAAG
CGTATTCTGTTTGAGGGCGCACAGGGCGCGCTTTTGGATATCGACTTTGGCACCTATCCCTTTGTAACCTCATCAAACGT
GATCGCAGGTCAGGCGGCCACCGGTGTTGGTGTCGGGCCGGGCGCGATCAACTATGTGCTCGGGATCGTCAAAGCCTATA
CAACGCGGGTGGGTGAAGGCCCCTTTCCGGCGGAACTGGATGATGCCGACGGCCAGCGTCTTGGCGAGCGCGGGCATGAA
TTTGGCACAGTGACAGGGCGCAAACGGCGCTGTGGCTGGTTTGATGCGGTGCTGGTGCGCCAGACCTGTGCGACATCCGG
TGTCAACGGGATTGCCCTGACCAAACTGGATGTTCTGGACGGATTCGAGACGCTCAAGATCTGTGTTGGATATGAGCTGG
ATGGCAAGCGCATGGATTATTTGCCGACCGCAGCGGACCATCAGGCACGCTGCCGTCCCATTTACGAAGAAATGCCGGGG
TGGTCGGATTCCACCGAAGGCGCGCGCAGTTGGGCGGATTTGCCCGCCAACGCGATTAAATATGTGCGCCGTGTTGAAGA
GTTGATCGACTGCCCGGTCGCTCTACTTTCTACTTCGCCGGAGCGGGAAGACACCATCCTCGTCACGGATCCGTTTGCCG
ATTGA

Upstream 100 bases:

>100_bases
TCAATCCGTCCGGTTGTATCCTTGCGCCGGAAGCGGGGCTTGCGGCTCGCACACGTCTATTACCGTTCCACGGTGCCGCG
TCAATTGAGCGGCCTGCATT

Downstream 100 bases:

>100_bases
TAGGGAACCCCATGTCGCTGAGTTACAAAGCCAGACGCCGCCTGACACTGCTGATCCTCCTGATTGGCTTACCGGTCTAT
ATCGTGTCCTGCATCACTGT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MRFRPGAGLAIRARVLSENKGPDMANVVVVGAQWGDEGKGKIVDWLSERADVIARFQGGHNAGHTLVIDGKVYKLNALPS
GVVRGGKLSVIGNGVVLDPWHLIKEIDIIRGQGVDISPETLMIAENTPLILPIHGELDRAREEAASKGTKIGTTGRGIGP
AYEDKVGRRSVRVADLADTATLEARVDRALQHHDPLRKGLGIEPVDRDALVAQLVEIAPHILKYAAPVWKVLNEKRRAGK
RILFEGAQGALLDIDFGTYPFVTSSNVIAGQAATGVGVGPGAINYVLGIVKAYTTRVGEGPFPAELDDADGQRLGERGHE
FGTVTGRKRRCGWFDAVLVRQTCATSGVNGIALTKLDVLDGFETLKICVGYELDGKRMDYLPTAADHQARCRPIYEEMPG
WSDSTEGARSWADLPANAIKYVRRVEELIDCPVALLSTSPEREDTILVTDPFAD

Sequences:

>Translated_454_residues
MRFRPGAGLAIRARVLSENKGPDMANVVVVGAQWGDEGKGKIVDWLSERADVIARFQGGHNAGHTLVIDGKVYKLNALPS
GVVRGGKLSVIGNGVVLDPWHLIKEIDIIRGQGVDISPETLMIAENTPLILPIHGELDRAREEAASKGTKIGTTGRGIGP
AYEDKVGRRSVRVADLADTATLEARVDRALQHHDPLRKGLGIEPVDRDALVAQLVEIAPHILKYAAPVWKVLNEKRRAGK
RILFEGAQGALLDIDFGTYPFVTSSNVIAGQAATGVGVGPGAINYVLGIVKAYTTRVGEGPFPAELDDADGQRLGERGHE
FGTVTGRKRRCGWFDAVLVRQTCATSGVNGIALTKLDVLDGFETLKICVGYELDGKRMDYLPTAADHQARCRPIYEEMPG
WSDSTEGARSWADLPANAIKYVRRVEELIDCPVALLSTSPEREDTILVTDPFAD
>Mature_454_residues
MRFRPGAGLAIRARVLSENKGPDMANVVVVGAQWGDEGKGKIVDWLSERADVIARFQGGHNAGHTLVIDGKVYKLNALPS
GVVRGGKLSVIGNGVVLDPWHLIKEIDIIRGQGVDISPETLMIAENTPLILPIHGELDRAREEAASKGTKIGTTGRGIGP
AYEDKVGRRSVRVADLADTATLEARVDRALQHHDPLRKGLGIEPVDRDALVAQLVEIAPHILKYAAPVWKVLNEKRRAGK
RILFEGAQGALLDIDFGTYPFVTSSNVIAGQAATGVGVGPGAINYVLGIVKAYTTRVGEGPFPAELDDADGQRLGERGHE
FGTVTGRKRRCGWFDAVLVRQTCATSGVNGIALTKLDVLDGFETLKICVGYELDGKRMDYLPTAADHQARCRPIYEEMPG
WSDSTEGARSWADLPANAIKYVRRVEELIDCPVALLSTSPEREDTILVTDPFAD

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=446, Percent_Identity=41.9282511210762, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI34577063, Length=425, Percent_Identity=40, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI40316944, Length=394, Percent_Identity=40.6091370558376, Blast_Score=279, Evalue=4e-75,
Organism=Escherichia coli, GI1790620, Length=430, Percent_Identity=50.2325581395349, Blast_Score=422, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI25146553, Length=428, Percent_Identity=43.4579439252336, Blast_Score=347, Evalue=6e-96,
Organism=Caenorhabditis elegans, GI25146555, Length=428, Percent_Identity=43.4579439252336, Blast_Score=347, Evalue=9e-96,
Organism=Saccharomyces cerevisiae, GI6324109, Length=429, Percent_Identity=38.9277389277389, Blast_Score=316, Evalue=4e-87,
Organism=Drosophila melanogaster, GI21356033, Length=423, Percent_Identity=41.6075650118203, Blast_Score=309, Evalue=2e-84,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 49015; Mature: 49015

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFRPGAGLAIRARVLSENKGPDMANVVVVGAQWGDEGKGKIVDWLSERADVIARFQGGH
CCCCCCCCCEEEEEECCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCC
NAGHTLVIDGKVYKLNALPSGVVRGGKLSVIGNGVVLDPWHLIKEIDIIRGQGVDISPET
CCCCEEEECCEEEEEECCCCHHCCCCEEEEEECCEEECHHHHHHHHHHHCCCCCCCCCCE
LMIAENTPLILPIHGELDRAREEAASKGTKIGTTGRGIGPAYEDKVGRRSVRVADLADTA
EEEECCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCEEEEEHHHHHH
TLEARVDRALQHHDPLRKGLGIEPVDRDALVAQLVEIAPHILKYAAPVWKVLNEKRRAGK
HHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RILFEGAQGALLDIDFGTYPFVTSSNVIAGQAATGVGVGPGAINYVLGIVKAYTTRVGEG
EEEEECCCCCEEEECCCCCCCEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
PFPAELDDADGQRLGERGHEFGTVTGRKRRCGWFDAVLVRQTCATSGVNGIALTKLDVLD
CCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHC
GFETLKICVGYELDGKRMDYLPTAADHQARCRPIYEEMPGWSDSTEGARSWADLPANAIK
CCCEEEEEEEEEECCCCCCCCCCCCCCCHHCCCHHHHCCCCCCCCHHHHHHHCCCHHHHH
YVRRVEELIDCPVALLSTSPEREDTILVTDPFAD
HHHHHHHHHHCCHHHHCCCCCCCCEEEEECCCCC
>Mature Secondary Structure
MRFRPGAGLAIRARVLSENKGPDMANVVVVGAQWGDEGKGKIVDWLSERADVIARFQGGH
CCCCCCCCCEEEEEECCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCC
NAGHTLVIDGKVYKLNALPSGVVRGGKLSVIGNGVVLDPWHLIKEIDIIRGQGVDISPET
CCCCEEEECCEEEEEECCCCHHCCCCEEEEEECCEEECHHHHHHHHHHHCCCCCCCCCCE
LMIAENTPLILPIHGELDRAREEAASKGTKIGTTGRGIGPAYEDKVGRRSVRVADLADTA
EEEECCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCEEEEEHHHHHH
TLEARVDRALQHHDPLRKGLGIEPVDRDALVAQLVEIAPHILKYAAPVWKVLNEKRRAGK
HHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RILFEGAQGALLDIDFGTYPFVTSSNVIAGQAATGVGVGPGAINYVLGIVKAYTTRVGEG
EEEEECCCCCEEEECCCCCCCEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
PFPAELDDADGQRLGERGHEFGTVTGRKRRCGWFDAVLVRQTCATSGVNGIALTKLDVLD
CCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHC
GFETLKICVGYELDGKRMDYLPTAADHQARCRPIYEEMPGWSDSTEGARSWADLPANAIK
CCCEEEEEEEEEECCCCCCCCCCCCCCCHHCCCHHHHCCCCCCCCHHHHHHHCCCHHHHH
YVRRVEELIDCPVALLSTSPEREDTILVTDPFAD
HHHHHHHHHHCCHHHHCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA