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The map label for this gene is nocT [H]

Identifier: 110679187

GI number: 110679187

Start: 1813344

End: 1814072

Strand: Reverse

Name: nocT [H]

Synonym: RD1_1898

Alternate gene names: 110679187

Gene position: 1814072-1813344 (Counterclockwise)

Preceding gene: 110679188

Following gene: 110679186

Centisome position: 43.89

GC content: 55.97

Gene sequence:

>729_bases
ATGAAAACACTTATCCTGACGACCGCCACACTGGCCCTGACAGCTGGTATGGCACTTGCGGATGGTCACGGCAAAACCGT
GCGCCTCGGCACGGAAGGCGCCTACCCTCCGTATAATTTCCTCAATGACGCCGGTGAAGTCGACGGGTTTGAGCGCGAAC
TTGGAGATGAACTCTGCGCGCGTGCCGAGCTGACCTGCGAATGGGTCACAAACGAATGGGACAGCATCATCCCCAACCTC
GTTTCCGGCAACTATGATGTCATCATCGCGGGTATGTCCGTTACGGATGAGCGCGATGAAGTTATTGATTTTTCGGAGCA
ATATACGCTTCCTGATCCCTCTGCCTATCTGGCGACCTCTGCGGATGTGGACCTTGAAAACGGTGTGATCGCTGCACAGG
CCAACACCATTCAGGCCAGCTTCATCGCCTCCTCTGGTGCAGTTCTGGTTGAATTCGCCACGCCCGAAGAAACCGTTTCT
GCCGTGAAAAATGGCGAAGCGGATGCGGTACTGGCGGACAAAGCCTTTCTGGCCGAACTGGCCAACGCCGATCCGGAGCT
GATGTTGCTGGAACAAAACGTCATGATCGGTGGCGGCATTGGTCTGGGCCTGCGCGAAAGCGACACGGAACTTAAGGAAA
AGTTCAACGTGGCCATCCGCTCCATGAAGGAAGACGGCAGCCTGAACGCCCTGATCGGCAAGTGGTTGCCCGATCTGGAA
CAGTTCTGA

Upstream 100 bases:

>100_bases
GCCGGAAACGCTGTTCGGCGCACCCAAATCGGAACGCCTGCGCGGGTTTCTCTCTGCGACCCAGGCAGCGTAAGCAAAAA
ATAAAAACGGGAGTACTAAT

Downstream 100 bases:

>100_bases
TTCACTCAAACCGGCGCGCGCCTCATCGTGCGCCGGTTTTCTTTCAAGCGAGTTAGATTATTTTCAGCTTTTGCACAGAC
CCGAGTATTCTGCCCGACTG

Product: periplasmic binding protein, putative

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MKTLILTTATLALTAGMALADGHGKTVRLGTEGAYPPYNFLNDAGEVDGFERELGDELCARAELTCEWVTNEWDSIIPNL
VSGNYDVIIAGMSVTDERDEVIDFSEQYTLPDPSAYLATSADVDLENGVIAAQANTIQASFIASSGAVLVEFATPEETVS
AVKNGEADAVLADKAFLAELANADPELMLLEQNVMIGGGIGLGLRESDTELKEKFNVAIRSMKEDGSLNALIGKWLPDLE
QF

Sequences:

>Translated_242_residues
MKTLILTTATLALTAGMALADGHGKTVRLGTEGAYPPYNFLNDAGEVDGFERELGDELCARAELTCEWVTNEWDSIIPNL
VSGNYDVIIAGMSVTDERDEVIDFSEQYTLPDPSAYLATSADVDLENGVIAAQANTIQASFIASSGAVLVEFATPEETVS
AVKNGEADAVLADKAFLAELANADPELMLLEQNVMIGGGIGLGLRESDTELKEKFNVAIRSMKEDGSLNALIGKWLPDLE
QF
>Mature_242_residues
MKTLILTTATLALTAGMALADGHGKTVRLGTEGAYPPYNFLNDAGEVDGFERELGDELCARAELTCEWVTNEWDSIIPNL
VSGNYDVIIAGMSVTDERDEVIDFSEQYTLPDPSAYLATSADVDLENGVIAAQANTIQASFIASSGAVLVEFATPEETVS
AVKNGEADAVLADKAFLAELANADPELMLLEQNVMIGGGIGLGLRESDTELKEKFNVAIRSMKEDGSLNALIGKWLPDLE
QF

Specific function: Component of the nopaline active transport system probably consisting of four subunits:Q, M, P and T. This system is also capable of transporting octopine provided that catabolic functions are induced with nopaline [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787088, Length=237, Percent_Identity=29.1139240506329, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1788649, Length=227, Percent_Identity=28.6343612334802, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1787085, Length=248, Percent_Identity=27.0161290322581, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1788648, Length=228, Percent_Identity=28.0701754385965, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1788228, Length=220, Percent_Identity=26.3636363636364, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1787031, Length=253, Percent_Identity=26.4822134387352, Blast_Score=71, Evalue=8e-14,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 25869; Mature: 25869

Theoretical pI: Translated: 3.77; Mature: 3.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLILTTATLALTAGMALADGHGKTVRLGTEGAYPPYNFLNDAGEVDGFERELGDELCA
CCEEEEEHHHHHHHCCCEEECCCCCEEEECCCCCCCCHHHHCCCCCCCHHHHHHHHHHHH
RAELTCEWVTNEWDSIIPNLVSGNYDVIIAGMSVTDERDEVIDFSEQYTLPDPSAYLATS
HHHEEEEECCCCHHHHHHHHHCCCEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCEEEEC
ADVDLENGVIAAQANTIQASFIASSGAVLVEFATPEETVSAVKNGEADAVLADKAFLAEL
CCCCCCCCEEEEECCEEEEEEEECCCCEEEEECCCHHHHHHHHCCCCCEEEHHHHHHHHH
ANADPELMLLEQNVMIGGGIGLGLRESDTELKEKFNVAIRSMKEDGSLNALIGKWLPDLE
HCCCCCEEEEECCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHH
QF
CC
>Mature Secondary Structure
MKTLILTTATLALTAGMALADGHGKTVRLGTEGAYPPYNFLNDAGEVDGFERELGDELCA
CCEEEEEHHHHHHHCCCEEECCCCCEEEECCCCCCCCHHHHCCCCCCCHHHHHHHHHHHH
RAELTCEWVTNEWDSIIPNLVSGNYDVIIAGMSVTDERDEVIDFSEQYTLPDPSAYLATS
HHHEEEEECCCCHHHHHHHHHCCCEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCEEEEC
ADVDLENGVIAAQANTIQASFIASSGAVLVEFATPEETVSAVKNGEADAVLADKAFLAEL
CCCCCCCCEEEEECCEEEEEEEECCCCEEEEECCCHHHHHHHHCCCCCEEEHHHHHHHHH
ANADPELMLLEQNVMIGGGIGLGLRESDTELKEKFNVAIRSMKEDGSLNALIGKWLPDLE
HCCCCCEEEEECCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHH
QF
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1732218; 11743193; 11743194 [H]