Click here to switch to the map view.

The map label for this gene is puuA [H]

Identifier: 110679184

GI number: 110679184

Start: 1810175

End: 1811509

Strand: Reverse

Name: puuA [H]

Synonym: RD1_1894

Alternate gene names: 110679184

Gene position: 1811509-1810175 (Counterclockwise)

Preceding gene: 110679185

Following gene: 110679183

Centisome position: 43.83

GC content: 62.47

Gene sequence:

>1335_bases
ATGAACAATTGGCTTCGAAAAAATCCACAGGTCCGCACGATCCGGGTGGCGGCGTCCGACCTCAACGGTCAGGCGCGCGG
CAAACGCATTCCGTCGCGCTATGCAGATAAAGCGGTGGAAGGCGGCACGCGGTTCCCCATGTCGGTTCTGAACCTCGACA
TCTGGGGTGAGGATATCGACGACAGCCCGCTGGTCTATGAAACCGGCGACCGCGACGGCGTGCTGAAACCGACCGAGCGG
GGGTTCATGCCCATGCCGTGGCTCGATGCGCCCTCGGCGCTTTTGCCGATCTGGATGTTTCACGAAGACGGGCGACCCTA
TGCAGGCGATCCCCGCCACGCGCTGAATGCGGTGGTTGAGCGCTATAGGGCGCGCGGGCTGACGCCGGTCTGTGCGGTGG
AGCTGGAGTTCTTCCTGATTGACGATTCCGGGCGCACGCTGCAGGTGCCACCATCGCCACGCTCCGGCAAGCGGCGCGTC
GCCGGAGAAATCCTGTCGCTGCGCGCGCTGGATCAGTTCGATGCGTTTTTCACCGACCTTTATGACGCCTGCGAGGAAAT
GGACATTCCGGCAGACACAGCGATTTCCGAAGCTGGGCTCGGCCAGTTCGAGATCAACCTGATGCATTGCGATGATGCGC
TCAGGGCGGCGGATGACGCGTGGCTGTTCAAGATGCTGGTCAAGGGCATGGCGCGGCGGCATGGGTTTGCCGCCAGTTTC
ATGGCCAAACCCTATGAAGATTACGCCGGGTCCGGCCTGCACATCCATTTCTCCGTGCTGGATGGCGCGGGCAACAATGT
CTTTGACGACGGGGGCGAGGCGGGCACCGATGTGCTGCGTCATGCGGTGGCGGGGTGCCTCAAGGCGATGCCCGGCTCGG
CCTTGGTGTTTGCGCCCCATGCCAACAGCTTTGATCGGATGGTGCCGGGATCGCATGCGCCCACTGGCATCAGTTGGGCC
TATGAAAACCGCACATCCGCCATACGGATTCCGTCAGGCAGCCCCGCGGCGCGGCGCATCGAACACCGGGTGGCGGGCGG
GGATGTGAACCCCTATCTGATGCTTGCCGCCGTTTTGGGTGCTGCCCTCAATGGGATCGAAGACGCGCAGGCCCCCCCCG
CCCCAATCACCGGGAATGCCTATGCCGCAGACCTGCCGCAAATCCCCGGCGACTGGCAAAGCGCGATTGACCTCTTTGAA
ACCGACGAACAGGTGGCGCGCATTTTTGCCCCCGACCTGATCCGCAATTTCGTGCTGACCAAACGGCAGGAGTTGAAATA
CATGGCCGAATTGTCGCCGCAGGAACAGGTCACCATCTATCTGGACACGGTGTAG

Upstream 100 bases:

>100_bases
CCTGATCCGGTAGTATCCATAATTTGATCAAATTATCCGTGGCGCTGGCCGTGGTACGGGACGCCGCACCCTTATGACCA
GCGCTCAAGACTGGTGCTGT

Downstream 100 bases:

>100_bases
AATATGAAGATTGGTATCCTGATCACCGGGCACCCGCCTGAAAACATGATGGAAGGCGGCAGTTACGACCGGTATTTTGA
GCGGTTGCTTGAGGGCCACG

Product: glutamine synthetase, putative

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MNNWLRKNPQVRTIRVAASDLNGQARGKRIPSRYADKAVEGGTRFPMSVLNLDIWGEDIDDSPLVYETGDRDGVLKPTER
GFMPMPWLDAPSALLPIWMFHEDGRPYAGDPRHALNAVVERYRARGLTPVCAVELEFFLIDDSGRTLQVPPSPRSGKRRV
AGEILSLRALDQFDAFFTDLYDACEEMDIPADTAISEAGLGQFEINLMHCDDALRAADDAWLFKMLVKGMARRHGFAASF
MAKPYEDYAGSGLHIHFSVLDGAGNNVFDDGGEAGTDVLRHAVAGCLKAMPGSALVFAPHANSFDRMVPGSHAPTGISWA
YENRTSAIRIPSGSPAARRIEHRVAGGDVNPYLMLAAVLGAALNGIEDAQAPPAPITGNAYAADLPQIPGDWQSAIDLFE
TDEQVARIFAPDLIRNFVLTKRQELKYMAELSPQEQVTIYLDTV

Sequences:

>Translated_444_residues
MNNWLRKNPQVRTIRVAASDLNGQARGKRIPSRYADKAVEGGTRFPMSVLNLDIWGEDIDDSPLVYETGDRDGVLKPTER
GFMPMPWLDAPSALLPIWMFHEDGRPYAGDPRHALNAVVERYRARGLTPVCAVELEFFLIDDSGRTLQVPPSPRSGKRRV
AGEILSLRALDQFDAFFTDLYDACEEMDIPADTAISEAGLGQFEINLMHCDDALRAADDAWLFKMLVKGMARRHGFAASF
MAKPYEDYAGSGLHIHFSVLDGAGNNVFDDGGEAGTDVLRHAVAGCLKAMPGSALVFAPHANSFDRMVPGSHAPTGISWA
YENRTSAIRIPSGSPAARRIEHRVAGGDVNPYLMLAAVLGAALNGIEDAQAPPAPITGNAYAADLPQIPGDWQSAIDLFE
TDEQVARIFAPDLIRNFVLTKRQELKYMAELSPQEQVTIYLDTV
>Mature_444_residues
MNNWLRKNPQVRTIRVAASDLNGQARGKRIPSRYADKAVEGGTRFPMSVLNLDIWGEDIDDSPLVYETGDRDGVLKPTER
GFMPMPWLDAPSALLPIWMFHEDGRPYAGDPRHALNAVVERYRARGLTPVCAVELEFFLIDDSGRTLQVPPSPRSGKRRV
AGEILSLRALDQFDAFFTDLYDACEEMDIPADTAISEAGLGQFEINLMHCDDALRAADDAWLFKMLVKGMARRHGFAASF
MAKPYEDYAGSGLHIHFSVLDGAGNNVFDDGGEAGTDVLRHAVAGCLKAMPGSALVFAPHANSFDRMVPGSHAPTGISWA
YENRTSAIRIPSGSPAARRIEHRVAGGDVNPYLMLAAVLGAALNGIEDAQAPPAPITGNAYAADLPQIPGDWQSAIDLFE
TDEQVARIFAPDLIRNFVLTKRQELKYMAELSPQEQVTIYLDTV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=432, Percent_Identity=25.462962962963, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI87081870, Length=437, Percent_Identity=36.3844393592677, Blast_Score=285, Evalue=6e-78,
Organism=Escherichia coli, GI1790301, Length=352, Percent_Identity=28.4090909090909, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 48641; Mature: 48641

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNWLRKNPQVRTIRVAASDLNGQARGKRIPSRYADKAVEGGTRFPMSVLNLDIWGEDID
CCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHEEEEECCCCCC
DSPLVYETGDRDGVLKPTERGFMPMPWLDAPSALLPIWMFHEDGRPYAGDPRHALNAVVE
CCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHEEEEEEECCCCCCCCCHHHHHHHHHH
RYRARGLTPVCAVELEFFLIDDSGRTLQVPPSPRSGKRRVAGEILSLRALDQFDAFFTDL
HHHHCCCCEEEEEEEEEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
YDACEEMDIPADTAISEAGLGQFEINLMHCDDALRAADDAWLFKMLVKGMARRHGFAASF
HHHHHHCCCCCHHHHHHCCCCEEEEEEEECCHHHHHCHHHHHHHHHHHHHHHHCCCHHHH
MAKPYEDYAGSGLHIHFSVLDGAGNNVFDDGGEAGTDVLRHAVAGCLKAMPGSALVFAPH
HCCCHHHHCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECC
ANSFDRMVPGSHAPTGISWAYENRTSAIRIPSGSPAARRIEHRVAGGDVNPYLMLAAVLG
CCCCCCCCCCCCCCCCCEEEECCCCCEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHH
AALNGIEDAQAPPAPITGNAYAADLPQIPGDWQSAIDLFETDEQVARIFAPDLIRNFVLT
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
KRQELKYMAELSPQEQVTIYLDTV
HHHHHHHHHHCCCCCEEEEEEECC
>Mature Secondary Structure
MNNWLRKNPQVRTIRVAASDLNGQARGKRIPSRYADKAVEGGTRFPMSVLNLDIWGEDID
CCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHEEEEECCCCCC
DSPLVYETGDRDGVLKPTERGFMPMPWLDAPSALLPIWMFHEDGRPYAGDPRHALNAVVE
CCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHEEEEEEECCCCCCCCCHHHHHHHHHH
RYRARGLTPVCAVELEFFLIDDSGRTLQVPPSPRSGKRRVAGEILSLRALDQFDAFFTDL
HHHHCCCCEEEEEEEEEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
YDACEEMDIPADTAISEAGLGQFEINLMHCDDALRAADDAWLFKMLVKGMARRHGFAASF
HHHHHHCCCCCHHHHHHCCCCEEEEEEEECCHHHHHCHHHHHHHHHHHHHHHHCCCHHHH
MAKPYEDYAGSGLHIHFSVLDGAGNNVFDDGGEAGTDVLRHAVAGCLKAMPGSALVFAPH
HCCCHHHHCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECC
ANSFDRMVPGSHAPTGISWAYENRTSAIRIPSGSPAARRIEHRVAGGDVNPYLMLAAVLG
CCCCCCCCCCCCCCCCCEEEECCCCCEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHH
AALNGIEDAQAPPAPITGNAYAADLPQIPGDWQSAIDLFETDEQVARIFAPDLIRNFVLT
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
KRQELKYMAELSPQEQVTIYLDTV
HHHHHHHHHHCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]