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The map label for this gene is metR [H]

Identifier: 110679061

GI number: 110679061

Start: 1691186

End: 1692091

Strand: Direct

Name: metR [H]

Synonym: RD1_1763

Alternate gene names: 110679061

Gene position: 1691186-1692091 (Clockwise)

Preceding gene: 110679058

Following gene: 110679066

Centisome position: 40.92

GC content: 60.6

Gene sequence:

>906_bases
ATGCATATTGAATTTCGCCATCTGCGCACGATCGAGGCGATCCATGAGGCCGGGGGCTTGGCGCGTGCGGCGGAACAGTT
GCATATCACGCAATCCGCTTTGAGTCATCAAATCAAGGGCTTGGAGGATCAGGCGGGGGTTGAGCTCTTCGTGCGCCGCT
CCAAACCGCTCAAGCTGTCTCCGGCGGGGATGCGCTTGCTGAAACTCGCGCGGCAGGTCTTGCCGCAGATCAAGGCATTG
CAGGATGAATTCACCGGCTTGCGCGCGGGCAGCACGGGGCGGATGCATATCGCCATTGAATGCCACGCCTGTTTTGAGTG
GCTGTTTCCCGTGCTGGAACAGTTTCGCAAGGCCTGGGGGGAGGTCGACGTGGACATCCGCCCCGGTCTGGCCTTTGATG
CGCTGCCCGCGCTGCTGAAGGAGGAAGTCGATCTGGTGGTGTCATCCGACCCCGAAGACCTGCCCGGTGTGGAATTTGTC
GAACTCTTTGATTATGCGCCGGTTTTCGTGGCCGCCAGCACCCATGCGCTGGCGCAGAAACCCTATGTCGAGGCGGGCGA
TTTTCGTGGTGAGACCCTGATCACCTATCCGGTGGAGCGTTCGCGTTTGGATGTGTTCAGCCAGTTGCTTATCCCGGCCA
AGGTCGAACCTGCGGCGATCCGGCAGGTGGAACTGACGGCCGTTATCCTGCTGTTGGTGGCGTCCAATCGGGGGGTCGCG
GTCTTGCCCGATTGGGTGGTGCGCGAGGTGAAGTATTCATCTGATTATGTCACGCGGCCCCTTACGGCCCAAGGGATCAC
GCGGCGTCTTTATGCGGCGGTGCGCAGTGATGATCTGGACAAGCCCTTCATGCAGCATTTGCTCGACCTCGCAGGGGAGG
AGGCGCGGAAACTGCAGTCGGCGTAA

Upstream 100 bases:

>100_bases
CTGGAGCGGGCATCGTTTTATCCTTGGGCTTTTCATGACTTGTGCCACAGAAGTAAAAATGAGACAAATTCATAACTTTC
ATCTTTAACATGAGTTCGGT

Downstream 100 bases:

>100_bases
GTCAAATGTGACGGACCCGCCCCGTTGCCCTTGTCACATATGGGTACAGGGTGCGGGGCGGGTATCTGGTCACCTGCAGC
GTCTGCTGAAAGACCTCTGT

Product: HTH-type transcriptional regulator metR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MHIEFRHLRTIEAIHEAGGLARAAEQLHITQSALSHQIKGLEDQAGVELFVRRSKPLKLSPAGMRLLKLARQVLPQIKAL
QDEFTGLRAGSTGRMHIAIECHACFEWLFPVLEQFRKAWGEVDVDIRPGLAFDALPALLKEEVDLVVSSDPEDLPGVEFV
ELFDYAPVFVAASTHALAQKPYVEAGDFRGETLITYPVERSRLDVFSQLLIPAKVEPAAIRQVELTAVILLLVASNRGVA
VLPDWVVREVKYSSDYVTRPLTAQGITRRLYAAVRSDDLDKPFMQHLLDLAGEEARKLQSA

Sequences:

>Translated_301_residues
MHIEFRHLRTIEAIHEAGGLARAAEQLHITQSALSHQIKGLEDQAGVELFVRRSKPLKLSPAGMRLLKLARQVLPQIKAL
QDEFTGLRAGSTGRMHIAIECHACFEWLFPVLEQFRKAWGEVDVDIRPGLAFDALPALLKEEVDLVVSSDPEDLPGVEFV
ELFDYAPVFVAASTHALAQKPYVEAGDFRGETLITYPVERSRLDVFSQLLIPAKVEPAAIRQVELTAVILLLVASNRGVA
VLPDWVVREVKYSSDYVTRPLTAQGITRRLYAAVRSDDLDKPFMQHLLDLAGEEARKLQSA
>Mature_301_residues
MHIEFRHLRTIEAIHEAGGLARAAEQLHITQSALSHQIKGLEDQAGVELFVRRSKPLKLSPAGMRLLKLARQVLPQIKAL
QDEFTGLRAGSTGRMHIAIECHACFEWLFPVLEQFRKAWGEVDVDIRPGLAFDALPALLKEEVDLVVSSDPEDLPGVEFV
ELFDYAPVFVAASTHALAQKPYVEAGDFRGETLITYPVERSRLDVFSQLLIPAKVEPAAIRQVELTAVILLLVASNRGVA
VLPDWVVREVKYSSDYVTRPLTAQGITRRLYAAVRSDDLDKPFMQHLLDLAGEEARKLQSA

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes. It is also a negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=294, Percent_Identity=43.8775510204082, Blast_Score=233, Evalue=8e-63,
Organism=Escherichia coli, GI1790208, Length=170, Percent_Identity=32.9411764705882, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI2367136, Length=242, Percent_Identity=25.2066115702479, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787879, Length=250, Percent_Identity=27.2, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI145693105, Length=304, Percent_Identity=24.0131578947368, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788887, Length=297, Percent_Identity=25.9259259259259, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789173, Length=188, Percent_Identity=29.7872340425532, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI157672245, Length=238, Percent_Identity=23.5294117647059, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787806, Length=244, Percent_Identity=22.9508196721311, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789506, Length=144, Percent_Identity=24.3055555555556, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790399, Length=193, Percent_Identity=26.4248704663212, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1786401, Length=135, Percent_Identity=27.4074074074074, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789204, Length=242, Percent_Identity=25.2066115702479, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788297, Length=251, Percent_Identity=27.0916334661355, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787530, Length=213, Percent_Identity=24.8826291079812, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33532; Mature: 33532

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIEFRHLRTIEAIHEAGGLARAAEQLHITQSALSHQIKGLEDQAGVELFVRRSKPLKLS
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEC
PAGMRLLKLARQVLPQIKALQDEFTGLRAGSTGRMHIAIECHACFEWLFPVLEQFRKAWG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHC
EVDVDIRPGLAFDALPALLKEEVDLVVSSDPEDLPGVEFVELFDYAPVFVAASTHALAQK
CCCEEECCCCHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHCCCCCEEEECHHHHHCC
PYVEAGDFRGETLITYPVERSRLDVFSQLLIPAKVEPAAIRQVELTAVILLLVASNRGVA
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEE
VLPDWVVREVKYSSDYVTRPLTAQGITRRLYAAVRSDDLDKPFMQHLLDLAGEEARKLQS
ECCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHCC
A
C
>Mature Secondary Structure
MHIEFRHLRTIEAIHEAGGLARAAEQLHITQSALSHQIKGLEDQAGVELFVRRSKPLKLS
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEC
PAGMRLLKLARQVLPQIKALQDEFTGLRAGSTGRMHIAIECHACFEWLFPVLEQFRKAWG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHC
EVDVDIRPGLAFDALPALLKEEVDLVVSSDPEDLPGVEFVELFDYAPVFVAASTHALAQK
CCCEEECCCCHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHCCCCCEEEECHHHHHCC
PYVEAGDFRGETLITYPVERSRLDVFSQLLIPAKVEPAAIRQVELTAVILLLVASNRGVA
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEE
VLPDWVVREVKYSSDYVTRPLTAQGITRRLYAAVRSDDLDKPFMQHLLDLAGEEARKLQS
ECCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]