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The map label for this gene is metF [H]

Identifier: 110679060

GI number: 110679060

Start: 1690229

End: 1691098

Strand: Reverse

Name: metF [H]

Synonym: RD1_1762

Alternate gene names: 110679060

Gene position: 1691098-1690229 (Counterclockwise)

Preceding gene: 110679062

Following gene: 110679059

Centisome position: 40.92

GC content: 62.87

Gene sequence:

>870_bases
ATGCCCGCTCCAGAAGTCTCATTCGAAGTTTTCCCGCCCCGCTCCATTGATGCATCGTTCAAGCTTTGGGAGACTGCGCA
GGCGCTTGCCCCTCTGGCGCCACGGTTTTTCTCGGTTACCTATGGCGCGGGCGGCACGACGCGCGACCTGACCCATGATG
CGGCCTATACATTGCACAAATCGTCCGGTCTGCCGGTCGCTGCGCATCTGACCTGCGTGGGGGCCTCCAAATCGGAAACA
CTCGCCATCGCGCAGCGTTTTGCCCAGGCCGGGATCACGGATATCGTCGCTCTGCGCGGCGACGCCCCCGAAGGCAGCAA
CGGCTTTACCCCTCACCCGGACGGGTTCGCCAACGCGGTGGAGCTGATCGACGCTCTCGCCCGCAGCGGCCAATTCACCC
TGCGCGTCGGCGCCTATCCCGAAAAACACCCCGAAGCCGCCAGCCAGCAGGCCAATATCGACTGGCTCAAGGCCAAGGTT
GACGCAGGCGCCTGCGAGGCGATCACGCAGTTTTTCTTTGAGGCCGATACCTTCCTGCGGTTTCGCGACGATTGCGTGCG
CGCGGGCATCTCGGTGCCGATCACACCCGGCATCCTGCCCATCACCAACTGGACGAATGCCCGCAAATTCGCGCAGCGCT
GCGGCACATCTATTCCCGCGTGGCTCGATCAGGCGTTTGACGCGGCCATTCGTGATGACCGGCACGACCTGCTGTCACGC
GCGCTGTGCACCGAACTCTGCTCGGATCTGGTGGGTGAGGGCGTCGACAAGCTGCATTTCTACACCCTGAACCGGCCAGA
GTTGACGCGCGAAATATGCCATGCCCTCGGTGTCACACAGGCCGTGACAGGCATTAAGGACGTCGCGTAA

Upstream 100 bases:

>100_bases
ATTCAATATGCATACCGAACTCATGTTAAAGATGAAAGTTATGAATTTGTCTCATTTTTACTTCTGTGGCACAAGTCATG
AAAAGCCCAAGGATAAAACG

Downstream 100 bases:

>100_bases
ACCTTGAAGCCGCGCCCCAGCCGCTTAAGCTCCCCGCAACACACTTTTGCGGAGAACCAGATGCCCTACGCTGCCCAAAC
CCCTGACGATTTGCTGCCCC

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MPAPEVSFEVFPPRSIDASFKLWETAQALAPLAPRFFSVTYGAGGTTRDLTHDAAYTLHKSSGLPVAAHLTCVGASKSET
LAIAQRFAQAGITDIVALRGDAPEGSNGFTPHPDGFANAVELIDALARSGQFTLRVGAYPEKHPEAASQQANIDWLKAKV
DAGACEAITQFFFEADTFLRFRDDCVRAGISVPITPGILPITNWTNARKFAQRCGTSIPAWLDQAFDAAIRDDRHDLLSR
ALCTELCSDLVGEGVDKLHFYTLNRPELTREICHALGVTQAVTGIKDVA

Sequences:

>Translated_289_residues
MPAPEVSFEVFPPRSIDASFKLWETAQALAPLAPRFFSVTYGAGGTTRDLTHDAAYTLHKSSGLPVAAHLTCVGASKSET
LAIAQRFAQAGITDIVALRGDAPEGSNGFTPHPDGFANAVELIDALARSGQFTLRVGAYPEKHPEAASQQANIDWLKAKV
DAGACEAITQFFFEADTFLRFRDDCVRAGISVPITPGILPITNWTNARKFAQRCGTSIPAWLDQAFDAAIRDDRHDLLSR
ALCTELCSDLVGEGVDKLHFYTLNRPELTREICHALGVTQAVTGIKDVA
>Mature_288_residues
PAPEVSFEVFPPRSIDASFKLWETAQALAPLAPRFFSVTYGAGGTTRDLTHDAAYTLHKSSGLPVAAHLTCVGASKSETL
AIAQRFAQAGITDIVALRGDAPEGSNGFTPHPDGFANAVELIDALARSGQFTLRVGAYPEKHPEAASQQANIDWLKAKVD
AGACEAITQFFFEADTFLRFRDDCVRAGISVPITPGILPITNWTNARKFAQRCGTSIPAWLDQAFDAAIRDDRHDLLSRA
LCTELCSDLVGEGVDKLHFYTLNRPELTREICHALGVTQAVTGIKDVA

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=285, Percent_Identity=35.0877192982456, Blast_Score=143, Evalue=2e-34,
Organism=Escherichia coli, GI1790377, Length=275, Percent_Identity=46.5454545454545, Blast_Score=244, Evalue=7e-66,
Organism=Caenorhabditis elegans, GI25147436, Length=281, Percent_Identity=32.3843416370107, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI25147441, Length=281, Percent_Identity=32.3843416370107, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6325234, Length=281, Percent_Identity=32.7402135231317, Blast_Score=131, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321313, Length=285, Percent_Identity=31.2280701754386, Blast_Score=123, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24662658, Length=238, Percent_Identity=26.4705882352941, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 31172; Mature: 31041

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAPEVSFEVFPPRSIDASFKLWETAQALAPLAPRFFSVTYGAGGTTRDLTHDAAYTLHK
CCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCHHEEEEECCCCCCCHHHHHHHHEEEC
SSGLPVAAHLTCVGASKSETLAIAQRFAQAGITDIVALRGDAPEGSNGFTPHPDGFANAV
CCCCCEEEEEEEECCCCHHHHHHHHHHHHCCHHHEEEECCCCCCCCCCCCCCCCHHHHHH
ELIDALARSGQFTLRVGAYPEKHPEAASQQANIDWLKAKVDAGACEAITQFFFEADTFLR
HHHHHHHHCCCEEEEECCCCCCCCCHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHH
FRDDCVRAGISVPITPGILPITNWTNARKFAQRCGTSIPAWLDQAFDAAIRDDRHDLLSR
HHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHH
ALCTELCSDLVGEGVDKLHFYTLNRPELTREICHALGVTQAVTGIKDVA
HHHHHHHHHHHHCCCHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PAPEVSFEVFPPRSIDASFKLWETAQALAPLAPRFFSVTYGAGGTTRDLTHDAAYTLHK
CCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCHHEEEEECCCCCCCHHHHHHHHEEEC
SSGLPVAAHLTCVGASKSETLAIAQRFAQAGITDIVALRGDAPEGSNGFTPHPDGFANAV
CCCCCEEEEEEEECCCCHHHHHHHHHHHHCCHHHEEEECCCCCCCCCCCCCCCCHHHHHH
ELIDALARSGQFTLRVGAYPEKHPEAASQQANIDWLKAKVDAGACEAITQFFFEADTFLR
HHHHHHHHCCCEEEEECCCCCCCCCHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHH
FRDDCVRAGISVPITPGILPITNWTNARKFAQRCGTSIPAWLDQAFDAAIRDDRHDLLSR
HHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHH
ALCTELCSDLVGEGVDKLHFYTLNRPELTREICHALGVTQAVTGIKDVA
HHHHHHHHHHHHCCCHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA