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The map label for this gene is mdh [H]

Identifier: 110678923

GI number: 110678923

Start: 1559670

End: 1560632

Strand: Reverse

Name: mdh [H]

Synonym: RD1_1617

Alternate gene names: 110678923

Gene position: 1560632-1559670 (Counterclockwise)

Preceding gene: 110678925

Following gene: 110678921

Centisome position: 37.76

GC content: 60.12

Gene sequence:

>963_bases
ATGGCAAGACCCAAAATCGCACTGATCGGCGCAGGCCAGATCGGCGGCACGCTCGCCCACCTCGCAGCCCTCAAGGAATT
GGGCGACGTTGTCCTCTTCGACATTGCCGAAGGAATCCCGGAAGGCAAAGCGCTGGACATCGCGGAAAGCGGCCCATCCG
CCAAATTCGACGCCAGAATGTCCGGCACACAGTCCTATGCGGATATCGCGGGGGCAGACGTCTGCATCGTCACCGCCGGT
GTCGCGCGCAAACCGGGCATGAGCCGCGATGACCTGCTGGGCATCAACCTCAAGGTCATGAAATCTGTCGGTGAAGGGAT
CGCGGCCCATGCGCCGAATGCCTTTGTGATCTGCATCACCAACCCGCTCGATGCGATGGTCTGGGCGCTGCGCGAATTCT
CCGGCCTGCCGCACGAAAAAGTCTGCGGCATGGCGGGTGTCCTTGATTCGGCGCGCTTCCGCCATTTCCTCGCGGATGAA
TTTGACGTCTCGATGAAAGACGTCACCGCCTTCGTTCTGGGCGGTCATGGCGACACGATGGTGCCCCTCGTGCGCTATTC
CACCGTTGCCGGCATTCCGCTGCCCGATCTGGTGAAAATGGGGTGGACCACACAGGATAAACTGGACGCGATCGTGCAAC
GCACCCGGGATGGCGGCGCTGAAATCGTCGGATTGCTGAAAACCGGTTCGGCCTTTTACGCACCCGCCACATCGGCTATC
GAAATGGCCGAAAGCTACCTCAAGGATCAGAAACGCGTGCTGCCCTGCGCGGCCTATGTCGACGGCGCTTATGGCCTCAA
GGGCTTTTACGTGGGCGTGCCAACCGTGATCGGCGCAGGTGGCGTTGAACGCGTGGTCGAAATCTCGATGAACAAAGATG
AACAGGCCATGTTCGACAACTCCGTCAACGCGGTTAAAGGACTTGTCGCAGCCTGCAAAGGCATCGACGACAGCCTGTCC
TGA

Upstream 100 bases:

>100_bases
CTGCGCCCATCATCAGGGCAATCGGTCGAGTAAACTTGCGCAACCCGCGTTCATTCGCTAGCAGGGCTACGAAATCAACC
GAACGCGGAGCATCTTCAAA

Downstream 100 bases:

>100_bases
TCCTGACAAAAGCGACCAAAACACCCCGGACATGTCCGGGGCGTTTTTTTGAGCGCAGCCTCATACTGTTTTGCCAAGAC
GGCGCAGCGGACACCGCAAA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MARPKIALIGAGQIGGTLAHLAALKELGDVVLFDIAEGIPEGKALDIAESGPSAKFDARMSGTQSYADIAGADVCIVTAG
VARKPGMSRDDLLGINLKVMKSVGEGIAAHAPNAFVICITNPLDAMVWALREFSGLPHEKVCGMAGVLDSARFRHFLADE
FDVSMKDVTAFVLGGHGDTMVPLVRYSTVAGIPLPDLVKMGWTTQDKLDAIVQRTRDGGAEIVGLLKTGSAFYAPATSAI
EMAESYLKDQKRVLPCAAYVDGAYGLKGFYVGVPTVIGAGGVERVVEISMNKDEQAMFDNSVNAVKGLVAACKGIDDSLS

Sequences:

>Translated_320_residues
MARPKIALIGAGQIGGTLAHLAALKELGDVVLFDIAEGIPEGKALDIAESGPSAKFDARMSGTQSYADIAGADVCIVTAG
VARKPGMSRDDLLGINLKVMKSVGEGIAAHAPNAFVICITNPLDAMVWALREFSGLPHEKVCGMAGVLDSARFRHFLADE
FDVSMKDVTAFVLGGHGDTMVPLVRYSTVAGIPLPDLVKMGWTTQDKLDAIVQRTRDGGAEIVGLLKTGSAFYAPATSAI
EMAESYLKDQKRVLPCAAYVDGAYGLKGFYVGVPTVIGAGGVERVVEISMNKDEQAMFDNSVNAVKGLVAACKGIDDSLS
>Mature_319_residues
ARPKIALIGAGQIGGTLAHLAALKELGDVVLFDIAEGIPEGKALDIAESGPSAKFDARMSGTQSYADIAGADVCIVTAGV
ARKPGMSRDDLLGINLKVMKSVGEGIAAHAPNAFVICITNPLDAMVWALREFSGLPHEKVCGMAGVLDSARFRHFLADEF
DVSMKDVTAFVLGGHGDTMVPLVRYSTVAGIPLPDLVKMGWTTQDKLDAIVQRTRDGGAEIVGLLKTGSAFYAPATSAIE
MAESYLKDQKRVLPCAAYVDGAYGLKGFYVGVPTVIGAGGVERVVEISMNKDEQAMFDNSVNAVKGLVAACKGIDDSLS

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=301, Percent_Identity=29.5681063122924, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI221136809, Length=301, Percent_Identity=29.5681063122924, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI9257228, Length=305, Percent_Identity=31.8032786885246, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI4504973, Length=305, Percent_Identity=31.8032786885246, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI5031857, Length=310, Percent_Identity=28.0645161290323, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI260099723, Length=310, Percent_Identity=28.0645161290323, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI15082234, Length=301, Percent_Identity=26.2458471760797, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI291575128, Length=305, Percent_Identity=28.1967213114754, Blast_Score=134, Evalue=8e-32,
Organism=Homo sapiens, GI4557032, Length=305, Percent_Identity=28.1967213114754, Blast_Score=134, Evalue=8e-32,
Organism=Homo sapiens, GI260099727, Length=198, Percent_Identity=30.8080808080808, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI260099725, Length=198, Percent_Identity=30.8080808080808, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI207028494, Length=193, Percent_Identity=28.4974093264249, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI21735621, Length=315, Percent_Identity=27.3015873015873, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI103472011, Length=292, Percent_Identity=18.8356164383562, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789632, Length=315, Percent_Identity=31.1111111111111, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17535107, Length=284, Percent_Identity=26.056338028169, Blast_Score=120, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17554310, Length=335, Percent_Identity=32.5373134328358, Blast_Score=103, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6322765, Length=225, Percent_Identity=27.5555555555556, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320125, Length=213, Percent_Identity=26.7605633802817, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324446, Length=252, Percent_Identity=28.1746031746032, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17136226, Length=313, Percent_Identity=27.1565495207668, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI45550422, Length=320, Percent_Identity=27.5, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24647881, Length=323, Percent_Identity=29.4117647058824, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24663595, Length=276, Percent_Identity=27.1739130434783, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24663599, Length=307, Percent_Identity=27.0358306188925, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 33507; Mature: 33376

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARPKIALIGAGQIGGTLAHLAALKELGDVVLFDIAEGIPEGKALDIAESGPSAKFDARM
CCCCCEEEEECCCHHHHHHHHHHHHHHCCEEEEHHHCCCCCCCEEEECCCCCCCCCCCCC
SGTQSYADIAGADVCIVTAGVARKPGMSRDDLLGINLKVMKSVGEGIAAHAPNAFVICIT
CCCCHHHHHCCCCEEEEECCCCCCCCCCCCCEECCHHHHHHHHCCCHHHCCCCEEEEEEC
NPLDAMVWALREFSGLPHEKVCGMAGVLDSARFRHFLADEFDVSMKDVTAFVLGGHGDTM
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCCC
VPLVRYSTVAGIPLPDLVKMGWTTQDKLDAIVQRTRDGGAEIVGLLKTGSAFYAPATSAI
HHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCHHHH
EMAESYLKDQKRVLPCAAYVDGAYGLKGFYVGVPTVIGAGGVERVVEISMNKDEQAMFDN
HHHHHHHHHHHHHCCHHHHHCCCCCCCEEEECCCHHHCCCCHHHHEEEECCCCHHHHHCC
SVNAVKGLVAACKGIDDSLS
HHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ARPKIALIGAGQIGGTLAHLAALKELGDVVLFDIAEGIPEGKALDIAESGPSAKFDARM
CCCCEEEEECCCHHHHHHHHHHHHHHCCEEEEHHHCCCCCCCEEEECCCCCCCCCCCCC
SGTQSYADIAGADVCIVTAGVARKPGMSRDDLLGINLKVMKSVGEGIAAHAPNAFVICIT
CCCCHHHHHCCCCEEEEECCCCCCCCCCCCCEECCHHHHHHHHCCCHHHCCCCEEEEEEC
NPLDAMVWALREFSGLPHEKVCGMAGVLDSARFRHFLADEFDVSMKDVTAFVLGGHGDTM
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCCC
VPLVRYSTVAGIPLPDLVKMGWTTQDKLDAIVQRTRDGGAEIVGLLKTGSAFYAPATSAI
HHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCHHHH
EMAESYLKDQKRVLPCAAYVDGAYGLKGFYVGVPTVIGAGGVERVVEISMNKDEQAMFDN
HHHHHHHHHHHHHCCHHHHHCCCCCCCEEEECCCHHHCCCCHHHHEEEECCCCHHHHHCC
SVNAVKGLVAACKGIDDSLS
HHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA