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The map label for this gene is sucB [H]

Identifier: 110678914

GI number: 110678914

Start: 1550270

End: 1551766

Strand: Reverse

Name: sucB [H]

Synonym: RD1_1608

Alternate gene names: 110678914

Gene position: 1551766-1550270 (Counterclockwise)

Preceding gene: 110678915

Following gene: 110678913

Centisome position: 37.54

GC content: 63.73

Gene sequence:

>1497_bases
ATGACCACTGAAGTACGCGTACCAACATTGGGCGAATCCGTCACCGAGGCCACCGTCGCCACCTGGTTCAAGAAGCCTGG
TGAGGCTGTGGCCGTGGACGAAATGCTCTGCGAATTGGAAACGGATAAGGTCACGGTCGAAGTGCCCTCGCCCATGGCGG
GCACATTGGGTGAGATCGTGGCAGGCGAAGGCGAAACCGTCGGCGTCAATGCGCTGCTTGCGACCATTTCCGAAGGGGCG
GCAGCACAGGCCCCGGCTGAGAATTCCGCCCCAACATCGGCTGCCGCATCCGCCTCCGTCGATGTGATGGTGCCGACGCT
CGGCGAGTCCGTGACCGAAGCAACGGTCAGCACGTGGTTCAAAGCCGTCGGCGACAGCGTGGCGCAGGATGAAATGCTCT
GCGAGCTGGAGACCGACAAGGTCAGCGTCGAGGTCCCCGCCCCCGCCGCCGGCACCCTGAGTGAAATCCTTGCACCCGAA
GGCGAGACCGTCGCCGCGGGAGGCAAACTGGCTGTCCTGTCTTCAGGCGATGGGGCCACCTCCGCCCCGGCAGCAGCCGC
CGCCACACCTGCCCCCGCAGCGCCCGCCGCAAGCGGGAGCAAGGACGTCGAAGACGCCCCATCCGCCAAGAAAGCCATGG
CGCAGGCCGGTCTGAGCCCTGATCAGGTCACCGGCACGGGCCGCGACGGGCGCATCATGAAGGAAGACGTAAGTCAGGCC
ATTGCTGCTGCGGCTTCGGCCCCAGCGCCTGCGGCGACTGCGCCCGCCCCGCGTGCCCCTGTCTCTGCCGATGATGCAAG
CCGCGAGGAGCGGGTGAAGATGACCCGTCTGCGCCAGACCATCGCCAAGCGCCTCAAGGACAGTCAGAACACCGCCGCCA
TGCTCACCACCTACAACGAGGTCGACATGACCGAGGTGATGGCCCTGCGCAACGAATACAAAGACCTGTTTCTCAAAAAG
CACGGGGTCAAGCTGGGGTTCATGTCCTTCTTTACCAAGGCCTGCTGCCACGCCCTGCGCGAGGTGCCCGAGGTCAACGC
AGAGATTGATGGCACGGATATCGTCTATAAGAACTTCGTGCATATGGGCATCGCGGCGGGCACGCCCACGGGCCTTGTGG
TGCCGGTCATTCGCGATGCGGATGCCATGTCGTTCCACGCCATCGAACAGGCGATTGCCGAAAAAGGCGCGCGCGCGCGC
GACGGCAAGCTGAGCATGGCGGAAATGCAGGGCGGGACCTTCACGATCTCGAACGGCGGGGTTTACGGCTCGCTGATGAG
TTCCCCGATCCTGAACCCGCCGCAATCGGGCATCCTAGGCATGCATAAAATTCAGGACCGCCCCATGGCGATCAATGGCC
AGGTCGTGATCCGCCCGATGATGTATCTGGCGCTCAGCTATGACCACCGGATCGTCGACGGCAAGGGTGCTGTGACCTTC
CTCGTGCGCGTGAAGGAAGCGCTGGAAGATCCACGGCGCTTGCTGATGGATTTGTGA

Upstream 100 bases:

>100_bases
CGCCACCTCCGCCTCGCCCGCGACCGGACTGGCCTCTCAGCACAACGCACAGCAAGCAGCCCTCGTGAACGAAGCGCTGA
CGATTGAAGGAAACTAAGAC

Downstream 100 bases:

>100_bases
TCTGCTGACTTTTGAAATCATGGAGCCGCGCCCAAACTCGGGCCACATGCCATCTTGCAAAAAGTCCAGCGGACCTTTTG
ACGTCGAAGGGGTCGTCAGC

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MTTEVRVPTLGESVTEATVATWFKKPGEAVAVDEMLCELETDKVTVEVPSPMAGTLGEIVAGEGETVGVNALLATISEGA
AAQAPAENSAPTSAAASASVDVMVPTLGESVTEATVSTWFKAVGDSVAQDEMLCELETDKVSVEVPAPAAGTLSEILAPE
GETVAAGGKLAVLSSGDGATSAPAAAAATPAPAAPAASGSKDVEDAPSAKKAMAQAGLSPDQVTGTGRDGRIMKEDVSQA
IAAAASAPAPAATAPAPRAPVSADDASREERVKMTRLRQTIAKRLKDSQNTAAMLTTYNEVDMTEVMALRNEYKDLFLKK
HGVKLGFMSFFTKACCHALREVPEVNAEIDGTDIVYKNFVHMGIAAGTPTGLVVPVIRDADAMSFHAIEQAIAEKGARAR
DGKLSMAEMQGGTFTISNGGVYGSLMSSPILNPPQSGILGMHKIQDRPMAINGQVVIRPMMYLALSYDHRIVDGKGAVTF
LVRVKEALEDPRRLLMDL

Sequences:

>Translated_498_residues
MTTEVRVPTLGESVTEATVATWFKKPGEAVAVDEMLCELETDKVTVEVPSPMAGTLGEIVAGEGETVGVNALLATISEGA
AAQAPAENSAPTSAAASASVDVMVPTLGESVTEATVSTWFKAVGDSVAQDEMLCELETDKVSVEVPAPAAGTLSEILAPE
GETVAAGGKLAVLSSGDGATSAPAAAAATPAPAAPAASGSKDVEDAPSAKKAMAQAGLSPDQVTGTGRDGRIMKEDVSQA
IAAAASAPAPAATAPAPRAPVSADDASREERVKMTRLRQTIAKRLKDSQNTAAMLTTYNEVDMTEVMALRNEYKDLFLKK
HGVKLGFMSFFTKACCHALREVPEVNAEIDGTDIVYKNFVHMGIAAGTPTGLVVPVIRDADAMSFHAIEQAIAEKGARAR
DGKLSMAEMQGGTFTISNGGVYGSLMSSPILNPPQSGILGMHKIQDRPMAINGQVVIRPMMYLALSYDHRIVDGKGAVTF
LVRVKEALEDPRRLLMDL
>Mature_497_residues
TTEVRVPTLGESVTEATVATWFKKPGEAVAVDEMLCELETDKVTVEVPSPMAGTLGEIVAGEGETVGVNALLATISEGAA
AQAPAENSAPTSAAASASVDVMVPTLGESVTEATVSTWFKAVGDSVAQDEMLCELETDKVSVEVPAPAAGTLSEILAPEG
ETVAAGGKLAVLSSGDGATSAPAAAAATPAPAAPAASGSKDVEDAPSAKKAMAQAGLSPDQVTGTGRDGRIMKEDVSQAI
AAAASAPAPAATAPAPRAPVSADDASREERVKMTRLRQTIAKRLKDSQNTAAMLTTYNEVDMTEVMALRNEYKDLFLKKH
GVKLGFMSFFTKACCHALREVPEVNAEIDGTDIVYKNFVHMGIAAGTPTGLVVPVIRDADAMSFHAIEQAIAEKGARARD
GKLSMAEMQGGTFTISNGGVYGSLMSSPILNPPQSGILGMHKIQDRPMAINGQVVIRPMMYLALSYDHRIVDGKGAVTFL
VRVKEALEDPRRLLMDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and l

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=232, Percent_Identity=59.051724137931, Blast_Score=300, Evalue=2e-81,
Organism=Homo sapiens, GI31711992, Length=570, Percent_Identity=28.4210526315789, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI110671329, Length=440, Percent_Identity=27.7272727272727, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI203098816, Length=480, Percent_Identity=25.8333333333333, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI203098753, Length=451, Percent_Identity=26.1640798226164, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=34.7826086956522, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI1786946, Length=406, Percent_Identity=50.4926108374384, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1786305, Length=528, Percent_Identity=31.25, Blast_Score=210, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI25146366, Length=403, Percent_Identity=45.9057071960298, Blast_Score=348, Evalue=4e-96,
Organism=Caenorhabditis elegans, GI17560088, Length=441, Percent_Identity=34.2403628117914, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17537937, Length=430, Percent_Identity=28.6046511627907, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17538894, Length=306, Percent_Identity=31.0457516339869, Blast_Score=137, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6320352, Length=404, Percent_Identity=42.5742574257426, Blast_Score=344, Evalue=2e-95,
Organism=Saccharomyces cerevisiae, GI6324258, Length=452, Percent_Identity=27.4336283185841, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24645909, Length=223, Percent_Identity=58.7443946188341, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI18859875, Length=438, Percent_Identity=28.310502283105, Blast_Score=152, Evalue=6e-37,
Organism=Drosophila melanogaster, GI20129315, Length=231, Percent_Identity=29.8701298701299, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24582497, Length=231, Percent_Identity=29.8701298701299, Blast_Score=109, Evalue=5e-24,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 51723; Mature: 51592

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTEVRVPTLGESVTEATVATWFKKPGEAVAVDEMLCELETDKVTVEVPSPMAGTLGEIV
CCCCEECCCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHCCCCEEEEECCCCCHHHHHHHH
AGEGETVGVNALLATISEGAAAQAPAENSAPTSAAASASVDVMVPTLGESVTEATVSTWF
CCCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCEEEEECCCCCHHHHHHHHHHH
KAVGDSVAQDEMLCELETDKVSVEVPAPAAGTLSEILAPEGETVAAGGKLAVLSSGDGAT
HHHCCHHHHHHHEEEEECCEEEEEECCCCCHHHHHHHCCCCCEEECCCEEEEEECCCCCC
SAPAAAAATPAPAAPAASGSKDVEDAPSAKKAMAQAGLSPDQVTGTGRDGRIMKEDVSQA
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHH
IAAAASAPAPAATAPAPRAPVSADDASREERVKMTRLRQTIAKRLKDSQNTAAMLTTYNE
HHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
VDMTEVMALRNEYKDLFLKKHGVKLGFMSFFTKACCHALREVPEVNAEIDGTDIVYKNFV
CHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
HMGIAAGTPTGLVVPVIRDADAMSFHAIEQAIAEKGARARDGKLSMAEMQGGTFTISNGG
HHHEECCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEHEEECCCEEEEECCC
VYGSLMSSPILNPPQSGILGMHKIQDRPMAINGQVVIRPMMYLALSYDHRIVDGKGAVTF
EEHHHHCCCCCCCCCCCCCCHHHHCCCCEEECCCCHHHHHHHHHHCCCCEEECCCCHHHH
LVRVKEALEDPRRLLMDL
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTEVRVPTLGESVTEATVATWFKKPGEAVAVDEMLCELETDKVTVEVPSPMAGTLGEIV
CCCEECCCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHCCCCEEEEECCCCCHHHHHHHH
AGEGETVGVNALLATISEGAAAQAPAENSAPTSAAASASVDVMVPTLGESVTEATVSTWF
CCCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCEEEEECCCCCHHHHHHHHHHH
KAVGDSVAQDEMLCELETDKVSVEVPAPAAGTLSEILAPEGETVAAGGKLAVLSSGDGAT
HHHCCHHHHHHHEEEEECCEEEEEECCCCCHHHHHHHCCCCCEEECCCEEEEEECCCCCC
SAPAAAAATPAPAAPAASGSKDVEDAPSAKKAMAQAGLSPDQVTGTGRDGRIMKEDVSQA
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHH
IAAAASAPAPAATAPAPRAPVSADDASREERVKMTRLRQTIAKRLKDSQNTAAMLTTYNE
HHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
VDMTEVMALRNEYKDLFLKKHGVKLGFMSFFTKACCHALREVPEVNAEIDGTDIVYKNFV
CHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
HMGIAAGTPTGLVVPVIRDADAMSFHAIEQAIAEKGARARDGKLSMAEMQGGTFTISNGG
HHHEECCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEHEEECCCEEEEECCC
VYGSLMSSPILNPPQSGILGMHKIQDRPMAINGQVVIRPMMYLALSYDHRIVDGKGAVTF
EEHHHHCCCCCCCCCCCCCCHHHHCCCCEEECCCCHHHHHHHHHHCCCCEEECCCCHHHH
LVRVKEALEDPRRLLMDL
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12874367 [H]