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The map label for this gene is lpdA [H]

Identifier: 110678909

GI number: 110678909

Start: 1547115

End: 1548503

Strand: Reverse

Name: lpdA [H]

Synonym: RD1_1603

Alternate gene names: 110678909

Gene position: 1548503-1547115 (Counterclockwise)

Preceding gene: 110678911

Following gene: 110678904

Centisome position: 37.47

GC content: 59.68

Gene sequence:

>1389_bases
ATGGCCAGCTACGACGTGATCATCATCGGCTCAGGCCCCGGCGGCTATGTCTGCGCCATTCGCTGCGCCCAACTGGGGCT
GAAAGTGGCCTGCGTCGAGGGGCGCGACACGCTGGGCGGCACCTGCCTGAATGTCGGGTGCATTCCATCCAAAGCGCTCC
TGCATGCCAGCCATATGCTGCACGAGGCAGAGCATAATTTCGCCGCCATGGGGCTGGTCGGCAAGAGCCCGTCGGTGGAT
TGGAAAAAGATGATGTCCTACAAGGAAGACACCATCGGCCAGAACACCAAGGGCATCGAATTCCTGTTCAAAAAGAACAA
GATCGACTGGCTGAAAGGTTGGGGGGCCATTCCTGAGGCCGGCAAAGTCAAGGTCGGGGATGAGGTGCATGAAGCCAAGC
ACATCATCATCGCCTCTGGCTCCGAACCCGCATCCCTGCCCGGTGTCGAGGTCGATGAGAAAACCGTCGTGACCTCAACC
GGCGCATTGGAACTGGGGAAGATCCCCAAGAAGATGGTGGTGATCGGTGCTGGCGTGATCGGGCTTGAACTGGGATCGGT
CTATGCGCGCCTCGGTGCCGAGGTCACGGTGGTTGAATTTTTGGACGCGATCACCCCCGGCATGGACGCCGAAGTGCAGA
AGACCTTCCAGAGAACGCTGAAAAAGCAGGGTCTTAAGTTCATCATGGGCGCTGCTGTACAAAAGACGGAGAGCCTCAAG
ACCAAGGCGAAAGTACACTACAAGCTGCGCAAGGACGACAGCGAACATGTGCTGGATGCCGATGTTGTTCTGGTGGCCAC
GGGCCGCAAACCCTTCACCGAAGGGCTGGGTCTGGACGCGCTGGGGGTCAAGATGTCCGCGCGGGGACAGATCGAGGTCA
ACGCCCATTGGGAAACCAATGTGCCGGGCATCTACGCGATCGGCGACGTGATCGACGGCCCGATGCTCGCCCACAAGGCC
GAGGATGAAGGGATGGCCGCCGCCGAACAGGTTGCGGGCAAACATGGGCACGTGAACTACGGCGTGATCCCCGGCGTCAT
CTATACCCACCCCGAAGTGGCCAACGTCGGCATGACCGAGGAAAACCTGAAAGAGGCGGGCCATGCCTATAAGGTCGGCA
AGTTCAGCTTCATGGGCAATGGCCGCGCGAAGGCGAATTTTGCCGGCGACGGTTTCGTCAAAATCATCGCCGACAAGGAC
ACGGATCGCATCCTTGGTGCGCATATCATCGGCCCGGCGGCAGGTGATTTGATCCATGAAATCTGCGTTGCCATGGAATT
CGGTGCCTCGGCAGAAGACCTCGCCATGACCTGCCATGCGCATCCGACCTACTCCGAAGCGGTCCGTGAGGCGGCACTGG
CCTGCGGCGATGGTCCGATTCACATGTGA

Upstream 100 bases:

>100_bases
ACCGACACGTCACCGATACCACTTTCAGGCGCGTCACCAACACCAGATCCGGACGCGTCACAGACACGATACCAACGCCT
GACAACAAAGGAAAAATACC

Downstream 100 bases:

>100_bases
TCCACGCCTGCGCGGGCTACCACATGGTTTCAGCAGCCCGCGCGCCCCATGCCTGATCATAGGCTGTGCCACCCTCGGCC
CCGTCGGTCATTTCCGACAG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MASYDVIIIGSGPGGYVCAIRCAQLGLKVACVEGRDTLGGTCLNVGCIPSKALLHASHMLHEAEHNFAAMGLVGKSPSVD
WKKMMSYKEDTIGQNTKGIEFLFKKNKIDWLKGWGAIPEAGKVKVGDEVHEAKHIIIASGSEPASLPGVEVDEKTVVTST
GALELGKIPKKMVVIGAGVIGLELGSVYARLGAEVTVVEFLDAITPGMDAEVQKTFQRTLKKQGLKFIMGAAVQKTESLK
TKAKVHYKLRKDDSEHVLDADVVLVATGRKPFTEGLGLDALGVKMSARGQIEVNAHWETNVPGIYAIGDVIDGPMLAHKA
EDEGMAAAEQVAGKHGHVNYGVIPGVIYTHPEVANVGMTEENLKEAGHAYKVGKFSFMGNGRAKANFAGDGFVKIIADKD
TDRILGAHIIGPAAGDLIHEICVAMEFGASAEDLAMTCHAHPTYSEAVREAALACGDGPIHM

Sequences:

>Translated_462_residues
MASYDVIIIGSGPGGYVCAIRCAQLGLKVACVEGRDTLGGTCLNVGCIPSKALLHASHMLHEAEHNFAAMGLVGKSPSVD
WKKMMSYKEDTIGQNTKGIEFLFKKNKIDWLKGWGAIPEAGKVKVGDEVHEAKHIIIASGSEPASLPGVEVDEKTVVTST
GALELGKIPKKMVVIGAGVIGLELGSVYARLGAEVTVVEFLDAITPGMDAEVQKTFQRTLKKQGLKFIMGAAVQKTESLK
TKAKVHYKLRKDDSEHVLDADVVLVATGRKPFTEGLGLDALGVKMSARGQIEVNAHWETNVPGIYAIGDVIDGPMLAHKA
EDEGMAAAEQVAGKHGHVNYGVIPGVIYTHPEVANVGMTEENLKEAGHAYKVGKFSFMGNGRAKANFAGDGFVKIIADKD
TDRILGAHIIGPAAGDLIHEICVAMEFGASAEDLAMTCHAHPTYSEAVREAALACGDGPIHM
>Mature_461_residues
ASYDVIIIGSGPGGYVCAIRCAQLGLKVACVEGRDTLGGTCLNVGCIPSKALLHASHMLHEAEHNFAAMGLVGKSPSVDW
KKMMSYKEDTIGQNTKGIEFLFKKNKIDWLKGWGAIPEAGKVKVGDEVHEAKHIIIASGSEPASLPGVEVDEKTVVTSTG
ALELGKIPKKMVVIGAGVIGLELGSVYARLGAEVTVVEFLDAITPGMDAEVQKTFQRTLKKQGLKFIMGAAVQKTESLKT
KAKVHYKLRKDDSEHVLDADVVLVATGRKPFTEGLGLDALGVKMSARGQIEVNAHWETNVPGIYAIGDVIDGPMLAHKAE
DEGMAAAEQVAGKHGHVNYGVIPGVIYTHPEVANVGMTEENLKEAGHAYKVGKFSFMGNGRAKANFAGDGFVKIIADKDT
DRILGAHIIGPAAGDLIHEICVAMEFGASAEDLAMTCHAHPTYSEAVREAALACGDGPIHM

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=462, Percent_Identity=53.2467532467532, Blast_Score=470, Evalue=1e-132,
Organism=Homo sapiens, GI50301238, Length=464, Percent_Identity=29.3103448275862, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI148277065, Length=431, Percent_Identity=27.8422273781903, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI33519430, Length=451, Percent_Identity=27.7161862527716, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI33519428, Length=451, Percent_Identity=27.7161862527716, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI33519426, Length=451, Percent_Identity=27.7161862527716, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI148277071, Length=428, Percent_Identity=27.803738317757, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI291045266, Length=429, Percent_Identity=28.4382284382284, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI22035672, Length=473, Percent_Identity=29.8097251585624, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI291045268, Length=423, Percent_Identity=27.4231678486998, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1786307, Length=451, Percent_Identity=41.4634146341463, Blast_Score=329, Evalue=2e-91,
Organism=Escherichia coli, GI87082354, Length=461, Percent_Identity=31.0195227765727, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1789915, Length=433, Percent_Identity=30.0230946882217, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI87081717, Length=461, Percent_Identity=27.5488069414317, Blast_Score=165, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI32565766, Length=460, Percent_Identity=55.2173913043478, Blast_Score=499, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17557007, Length=474, Percent_Identity=26.3713080168776, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI71982272, Length=495, Percent_Identity=25.0505050505051, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71983429, Length=441, Percent_Identity=25.6235827664399, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71983419, Length=441, Percent_Identity=25.6235827664399, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17559934, Length=186, Percent_Identity=27.9569892473118, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=473, Percent_Identity=50.3171247357294, Blast_Score=450, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6325240, Length=479, Percent_Identity=31.7327766179541, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6325166, Length=468, Percent_Identity=29.9145299145299, Blast_Score=184, Evalue=3e-47,
Organism=Drosophila melanogaster, GI21358499, Length=466, Percent_Identity=54.7210300429184, Blast_Score=490, Evalue=1e-138,
Organism=Drosophila melanogaster, GI24640553, Length=479, Percent_Identity=30.062630480167, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24640549, Length=479, Percent_Identity=30.062630480167, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24640551, Length=478, Percent_Identity=30.1255230125523, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI17737741, Length=474, Percent_Identity=28.4810126582279, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48940; Mature: 48809

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASYDVIIIGSGPGGYVCAIRCAQLGLKVACVEGRDTLGGTCLNVGCIPSKALLHASHML
CCCEEEEEEECCCCCEEEEEEHHHCCEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHH
HEAEHNFAAMGLVGKSPSVDWKKMMSYKEDTIGQNTKGIEFLFKKNKIDWLKGWGAIPEA
HHHCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHCCCCCCCC
GKVKVGDEVHEAKHIIIASGSEPASLPGVEVDEKTVVTSTGALELGKIPKKMVVIGAGVI
CCEEECCHHHCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCEECCCCCCEEEEEECCHH
GLELGSVYARLGAEVTVVEFLDAITPGMDAEVQKTFQRTLKKQGLKFIMGAAVQKTESLK
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
TKAKVHYKLRKDDSEHVLDADVVLVATGRKPFTEGLGLDALGVKMSARGQIEVNAHWETN
HHEEEEEEEECCCCCCEECCCEEEEEECCCCCHHCCCCCEECEEEECCCEEEEEEEECCC
VPGIYAIGDVIDGPMLAHKAEDEGMAAAEQVAGKHGHVNYGVIPGVIYTHPEVANVGMTE
CCCEEEEHHHCCCCHHEECCCCCCHHHHHHHCCCCCCEECCCCCCEEEECCCCCCCCCCH
ENLKEAGHAYKVGKFSFMGNGRAKANFAGDGFVKIIADKDTDRILGAHIIGPAAGDLIHE
HHHHHCCCEEEEEEEEEECCCCEEEEECCCCEEEEEECCCCCCEEEEEEECCCHHHHHHH
ICVAMEFGASAEDLAMTCHAHPTYSEAVREAALACGDGPIHM
HHHHHHCCCCHHHEEEEEECCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ASYDVIIIGSGPGGYVCAIRCAQLGLKVACVEGRDTLGGTCLNVGCIPSKALLHASHML
CCEEEEEEECCCCCEEEEEEHHHCCEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHH
HEAEHNFAAMGLVGKSPSVDWKKMMSYKEDTIGQNTKGIEFLFKKNKIDWLKGWGAIPEA
HHHCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHCCCCCCCC
GKVKVGDEVHEAKHIIIASGSEPASLPGVEVDEKTVVTSTGALELGKIPKKMVVIGAGVI
CCEEECCHHHCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCEECCCCCCEEEEEECCHH
GLELGSVYARLGAEVTVVEFLDAITPGMDAEVQKTFQRTLKKQGLKFIMGAAVQKTESLK
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
TKAKVHYKLRKDDSEHVLDADVVLVATGRKPFTEGLGLDALGVKMSARGQIEVNAHWETN
HHEEEEEEEECCCCCCEECCCEEEEEECCCCCHHCCCCCEECEEEECCCEEEEEEEECCC
VPGIYAIGDVIDGPMLAHKAEDEGMAAAEQVAGKHGHVNYGVIPGVIYTHPEVANVGMTE
CCCEEEEHHHCCCCHHEECCCCCCHHHHHHHCCCCCCEECCCCCCEEEECCCCCCCCCCH
ENLKEAGHAYKVGKFSFMGNGRAKANFAGDGFVKIIADKDTDRILGAHIIGPAAGDLIHE
HHHHHCCCEEEEEEEEEECCCCEEEEECCCCEEEEEECCCCCCEEEEEEECCCHHHHHHH
ICVAMEFGASAEDLAMTCHAHPTYSEAVREAALACGDGPIHM
HHHHHHCCCCHHHEEEEEECCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9226266 [H]