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The map label for this gene is gph [C]

Identifier: 110678759

GI number: 110678759

Start: 1371028

End: 1371705

Strand: Direct

Name: gph [C]

Synonym: RD1_1442

Alternate gene names: 110678759

Gene position: 1371028-1371705 (Clockwise)

Preceding gene: 110678758

Following gene: 110678760

Centisome position: 33.17

GC content: 63.42

Gene sequence:

>678_bases
ATGCCCACGCCGCTGCGATTGGTGATATTCGATGTGGATGGCACTTTGGTGGACAGCCAGGGCGACATTCTGGGCGCGAT
GCGCCATGCCTTCGAGGGTCTGGGCCTTGATGTGCCGTCGCGCGATGCGGTGTTGGGCATTGTGGGCCTGTCCTTACCCG
TCGCCATGGCGCGTCTGGCGCCGGATGTGGGCCCGGCGCTGCAGAGCCAATTGGTGGAGGGGTATAAATCCGCCTATGTT
GACATGCGCGCCAAGGTTGGCGCGGCGCAGTCCAGCCCGCTTTATCCCGGTGCGCGGGGCAGTCTGGAGCAGTTGCACAG
CACGCCCGAGGTCTTGCTGGGCGTGGCGACGGGCAAGTCCAAGCGCGGGCTCGACAGTCTGATCAAGGCCCATGATCTGG
GGTCGTTTTTCGTCACACGGCAGGTGGCAGATTTTCACCCGTCCAAACCGCATCCGTCGATGATCCTGCAGGCGATGGCG
GACACCGGGGTTGATCCGGAGAACACGGTCATGATCGGGGACACAAGTTTCGATATGGAGATGGCCCGGTCCGCTGGGGT
GCACGGCATTGGCGTCAGTTGGGGGTATCATCCGGTTTCGGCGCTGACGGGCGCGGCGGAGGTTCTGACGGATTTTACCG
GGCTGCCCCCGGCACTCTCGCGACTTTGGGGAGAATGA

Upstream 100 bases:

>100_bases
TCACGATCACGGCGGATATGCCGGACCATATGAAACAGAGCTGGGAGACCTTTGGCTGGACCGAGGATCTGGCCGCCGAA
GACCCGTTCGAGGCGCTTTG

Downstream 100 bases:

>100_bases
AACATGAGTGAATGGAAGGCAAAACGCTTCTGGAAAGAGGCCGTGATTGATGAAACGGCAGAGGGGTTTGGCATTGCGCT
GGATGGGCGCGCAGTCAAAA

Product: hydrolase, putative

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MPTPLRLVIFDVDGTLVDSQGDILGAMRHAFEGLGLDVPSRDAVLGIVGLSLPVAMARLAPDVGPALQSQLVEGYKSAYV
DMRAKVGAAQSSPLYPGARGSLEQLHSTPEVLLGVATGKSKRGLDSLIKAHDLGSFFVTRQVADFHPSKPHPSMILQAMA
DTGVDPENTVMIGDTSFDMEMARSAGVHGIGVSWGYHPVSALTGAAEVLTDFTGLPPALSRLWGE

Sequences:

>Translated_225_residues
MPTPLRLVIFDVDGTLVDSQGDILGAMRHAFEGLGLDVPSRDAVLGIVGLSLPVAMARLAPDVGPALQSQLVEGYKSAYV
DMRAKVGAAQSSPLYPGARGSLEQLHSTPEVLLGVATGKSKRGLDSLIKAHDLGSFFVTRQVADFHPSKPHPSMILQAMA
DTGVDPENTVMIGDTSFDMEMARSAGVHGIGVSWGYHPVSALTGAAEVLTDFTGLPPALSRLWGE
>Mature_224_residues
PTPLRLVIFDVDGTLVDSQGDILGAMRHAFEGLGLDVPSRDAVLGIVGLSLPVAMARLAPDVGPALQSQLVEGYKSAYVD
MRAKVGAAQSSPLYPGARGSLEQLHSTPEVLLGVATGKSKRGLDSLIKAHDLGSFFVTRQVADFHPSKPHPSMILQAMAD
TGVDPENTVMIGDTSFDMEMARSAGVHGIGVSWGYHPVSALTGAAEVLTDFTGLPPALSRLWGE

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1788021, Length=199, Percent_Identity=30.6532663316583, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 23628; Mature: 23497

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTPLRLVIFDVDGTLVDSQGDILGAMRHAFEGLGLDVPSRDAVLGIVGLSLPVAMARLA
CCCCEEEEEEECCCEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHC
PDVGPALQSQLVEGYKSAYVDMRAKVGAAQSSPLYPGARGSLEQLHSTPEVLLGVATGKS
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCHHHEEEECCCCC
KRGLDSLIKAHDLGSFFVTRQVADFHPSKPHPSMILQAMADTGVDPENTVMIGDTSFDME
CCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCHH
MARSAGVHGIGVSWGYHPVSALTGAAEVLTDFTGLPPALSRLWGE
HHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCC
>Mature Secondary Structure 
PTPLRLVIFDVDGTLVDSQGDILGAMRHAFEGLGLDVPSRDAVLGIVGLSLPVAMARLA
CCCEEEEEEECCCEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHC
PDVGPALQSQLVEGYKSAYVDMRAKVGAAQSSPLYPGARGSLEQLHSTPEVLLGVATGKS
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCHHHEEEECCCCC
KRGLDSLIKAHDLGSFFVTRQVADFHPSKPHPSMILQAMADTGVDPENTVMIGDTSFDME
CCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCHH
MARSAGVHGIGVSWGYHPVSALTGAAEVLTDFTGLPPALSRLWGE
HHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]