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The map label for this gene is rpoA [H]

Identifier: 110678754

GI number: 110678754

Start: 1366413

End: 1367441

Strand: Direct

Name: rpoA [H]

Synonym: RD1_1437

Alternate gene names: 110678754

Gene position: 1366413-1367441 (Clockwise)

Preceding gene: 110678753

Following gene: 110678755

Centisome position: 33.06

GC content: 56.37

Gene sequence:

>1029_bases
ATGGAGGGACGCATGATCCACAAGAACTGGGCTGAATTGATCAAGCCGCAACAACTTGACGTCAAACCGGGCAATGATCC
GGCACGTCAGGCAACCGTCACGGCGGAACCGCTGGAGCGCGGCTTTGGCCTGACCATGGGCAACGCGCTGCGTCGTGTTC
TGATGTCGTCGCTGCAGGGGGCCGCGATCACCTCTGTGCAGATCGACAATGTATTGCACGAGTTTTCATCGGTGGCCGGT
GTGCGGGAAGACGTCACTGACATCATCCTGAACCTCAAGGGCGTTTCCATCCGTATGGAAGTCGAAGGGCCAAAGCGGCT
GTCGATCTCCGCCAAGGGGCCGGGTGTTGTGACTGCTGGTGACATTTCGGAATCTGCGGGCATCGAAATCCTGAACCGGG
ATCATGTGATCTGCCACCTTGATGACGGTGCCGACGTTTACATGGAACTGACCGTCAATCAGGGTAAAGGCTATGTTTCT
GCCGAAAAGAACAAGCCAGAGGACGCACCCATTGGCCTGATCCCGATTGATGCGATCTATTCGCCGGTCAAGAAGGTCAG
CTATGACGTGCAACCGACCCGCGAAGGTCAGGTGCTGGATTATGACAAGCTGACCATGAAGGTCGAAACCGATGGGTCAC
TGACGCCGGATGATGCGGTGGCGTTCGCCGCGCGCATTCTGCAGGACCAGCTGGGCATCTTCGTCAACTTCGAAGAGCCT
GAATCCGCCTCCCGCGCGGATGAGGACGACGGTCTGGAGTTCAACCCGCTGCTGCTCAAGAAAGTGGACGACTTGGAACT
GTCGGTACGTTCTGCAAACTGTCTGAAGAATGACAACATCGTCTACATCGGTGATCTCATTCAGAAGACCGAAGCGGAGA
TGCTGCGCACGCCGAACTTTGGGCGTAAGTCGCTCAATGAGATCAAGGAAGTGCTCTCGGGCATGGGTCTGCATCTCGGC
ATGGACGTCGAGGACTGGCCGCCGGACAACATCGAAGACCTGGCCAAGAAATTCGAAGACTCTTTCTAA

Upstream 100 bases:

>100_bases
TGTTTTACCAGATTTGGGGCCGTGTGTTCGCGCACGGCCCCCATCCGCTTTTTTGAAACCTCGAGCGTCTGCACCTTTCG
GACATGGGGCGCAGACAAGA

Downstream 100 bases:

>100_bases
GGTCCGACCAATGCCCGCAGGTGCGGGGACGATCCGGGTCGCAAGGCCCCAAGGAGAACCGGGTGACACGCATCACCCGG
TCAGACAAAGCAAAACGGCC

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MEGRMIHKNWAELIKPQQLDVKPGNDPARQATVTAEPLERGFGLTMGNALRRVLMSSLQGAAITSVQIDNVLHEFSSVAG
VREDVTDIILNLKGVSIRMEVEGPKRLSISAKGPGVVTAGDISESAGIEILNRDHVICHLDDGADVYMELTVNQGKGYVS
AEKNKPEDAPIGLIPIDAIYSPVKKVSYDVQPTREGQVLDYDKLTMKVETDGSLTPDDAVAFAARILQDQLGIFVNFEEP
ESASRADEDDGLEFNPLLLKKVDDLELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLSGMGLHLG
MDVEDWPPDNIEDLAKKFEDSF

Sequences:

>Translated_342_residues
MEGRMIHKNWAELIKPQQLDVKPGNDPARQATVTAEPLERGFGLTMGNALRRVLMSSLQGAAITSVQIDNVLHEFSSVAG
VREDVTDIILNLKGVSIRMEVEGPKRLSISAKGPGVVTAGDISESAGIEILNRDHVICHLDDGADVYMELTVNQGKGYVS
AEKNKPEDAPIGLIPIDAIYSPVKKVSYDVQPTREGQVLDYDKLTMKVETDGSLTPDDAVAFAARILQDQLGIFVNFEEP
ESASRADEDDGLEFNPLLLKKVDDLELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLSGMGLHLG
MDVEDWPPDNIEDLAKKFEDSF
>Mature_342_residues
MEGRMIHKNWAELIKPQQLDVKPGNDPARQATVTAEPLERGFGLTMGNALRRVLMSSLQGAAITSVQIDNVLHEFSSVAG
VREDVTDIILNLKGVSIRMEVEGPKRLSISAKGPGVVTAGDISESAGIEILNRDHVICHLDDGADVYMELTVNQGKGYVS
AEKNKPEDAPIGLIPIDAIYSPVKKVSYDVQPTREGQVLDYDKLTMKVETDGSLTPDDAVAFAARILQDQLGIFVNFEEP
ESASRADEDDGLEFNPLLLKKVDDLELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLSGMGLHLG
MDVEDWPPDNIEDLAKKFEDSF

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family [H]

Homologues:

Organism=Escherichia coli, GI1789690, Length=327, Percent_Identity=47.7064220183486, Blast_Score=311, Evalue=6e-86,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- ProDom:   PD001179 [H]

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 37560; Mature: 37560

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGRMIHKNWAELIKPQQLDVKPGNDPARQATVTAEPLERGFGLTMGNALRRVLMSSLQG
CCCCEEHHHHHHHCCCCEECCCCCCCHHHEEEEEHHHHHCCCCCCHHHHHHHHHHHHCCC
AAITSVQIDNVLHEFSSVAGVREDVTDIILNLKGVSIRMEVEGPKRLSISAKGPGVVTAG
CEEEEEHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCCEEEEC
DISESAGIEILNRDHVICHLDDGADVYMELTVNQGKGYVSAEKNKPEDAPIGLIPIDAIY
CCCCCCCEEEECCCEEEEEECCCCEEEEEEEEECCCCEEECCCCCCCCCCEEEEEHHHHH
SPVKKVSYDVQPTREGQVLDYDKLTMKVETDGSLTPDDAVAFAARILQDQLGIFVNFEEP
HHHHHHCCCCCCCCCCCEEEECEEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEECCC
ESASRADEDDGLEFNPLLLKKVDDLELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNF
CCCCCCCCCCCCEECCHHEEECCCCEEEEECCCCCCCCCEEEEHHHHHHHHHHHHCCCCC
GRKSLNEIKEVLSGMGLHLGMDVEDWPPDNIEDLAKKFEDSF
CHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MEGRMIHKNWAELIKPQQLDVKPGNDPARQATVTAEPLERGFGLTMGNALRRVLMSSLQG
CCCCEEHHHHHHHCCCCEECCCCCCCHHHEEEEEHHHHHCCCCCCHHHHHHHHHHHHCCC
AAITSVQIDNVLHEFSSVAGVREDVTDIILNLKGVSIRMEVEGPKRLSISAKGPGVVTAG
CEEEEEHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCCEEEEC
DISESAGIEILNRDHVICHLDDGADVYMELTVNQGKGYVSAEKNKPEDAPIGLIPIDAIY
CCCCCCCEEEECCCEEEEEECCCCEEEEEEEEECCCCEEECCCCCCCCCCEEEEEHHHHH
SPVKKVSYDVQPTREGQVLDYDKLTMKVETDGSLTPDDAVAFAARILQDQLGIFVNFEEP
HHHHHHCCCCCCCCCCCEEEECEEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEECCC
ESASRADEDDGLEFNPLLLKKVDDLELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNF
CCCCCCCCCCCCEECCHHEEECCCCEEEEECCCCCCCCCEEEEHHHHHHHHHHHHCCCCC
GRKSLNEIKEVLSGMGLHLGMDVEDWPPDNIEDLAKKFEDSF
CHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA