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The map label for this gene is tufA [H]

Identifier: 110678715

GI number: 110678715

Start: 1339709

End: 1340884

Strand: Direct

Name: tufA [H]

Synonym: RD1_1396

Alternate gene names: 110678715

Gene position: 1339709-1340884 (Clockwise)

Preceding gene: 110678714

Following gene: 110678717

Centisome position: 32.41

GC content: 60.29

Gene sequence:

>1176_bases
ATGGCAAAGGCAAAGTTTGAACGTAATAAACCGCATGTTAACATTGGCACGATTGGTCACGTTGACCACGGCAAGACGAC
GCTGACGGCTGCGATTACGAAGTATTTCGGTGATTTTCAGGCGTATGACCAGATTGACGGCGCGCCTGAGGAGAAAGCGC
GCGGGATCACGATTTCGACCGCGCATGTGGAATACGAGACGGAAAACCGTCACTACGCGCATGTGGATTGCCCCGGTCAC
GCGGACTACGTCAAGAACATGATCACCGGTGCGGCGCAGATGGACGGCGCGATCCTGGTTGTGAACGCGGCTGACGGCCC
GATGCCCCAGACGCGCGAGCACATCCTGCTGGGTCGCCAGGTTGGCATCCCGACGATGGTTGTCTTCATGAACAAGGTCG
ATCAGGTCGATGACGAGGAACTGCTTGAGCTGGTCGAGATGGAAATCCGCGAGCTGCTGTCCTCCTACGACTACCCCGGC
GACGACATTCCCGTGATCCCCGGCTCCGCGCTGGCGGCGATGGAAGGGCGTGATCCCGAGATCGGTGAAGAGGCGATCAA
GAAGCTGATGGCGGCTGTGGATGAGTTCATCCCGACGCCTGAGCGTGCGATTGACCAGCCGTTCCTGATGCCGGTTGAGG
ACGTCTTCTCGATCTCCGGTCGTGGTACTGTTGTGACGGGCCGTGTTGAGCGTGGCGTGATCAACGTGGGCGACAACATC
GAGATCGTGGGGATCAAGGACACCCAGAGCACAACATGTACGGGTGTTGAGATGTTCCGCAAGCTGCTGGATCGCGGTGA
GGCGGGCGACAACATCGGCGCGCTGCTGCGTGGTATTGACCGTGAGGCGGTTGAGCGTGGCCAGGTTCTGTGCAAGCCGG
GGTCGGTCAAGCCACACACGAAGTTCGAGGCCGAAGCCTATATCCTGACCAAGGACGAAGGTGGCCGCCACACGCCGTTC
TTTGCGAACTACCGCCCGCAGTTCTACTTCCGCACAACGGACGTGACGGGCACGGTTCAGCTGGCGGAAGGCACCGAGAT
GGTCATGCCGGGCGACAACGTGTCCTTTGGCGTTGAACTGATCGCACCGATCGCGATGGAGCAGGGCCTGCGCTTTGCGA
TCCGCGAAGGCGGCCGCACCGTCGGCGCCGGCGTCGTGTCGAAGATTACGGAGTAA

Upstream 100 bases:

>100_bases
CGCGCTTGATTTTCCCGTCAGTTCGCAATACATCGCCCGCGTTACCAAATGGACCGGGTCGCAGATCGTGACCCTCTGAC
AACAAAAGGGCGCCACGATC

Downstream 100 bases:

>100_bases
TCCCGAGACGCGCCCTCAAAAGCGTGTCGAAGATTACGGAGTAATCCCGAGACGCGCCCCCAAAAGCGTGTCGAAGATTA
CGGAGTAATCCCGAGACGCG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MAKAKFERNKPHVNIGTIGHVDHGKTTLTAAITKYFGDFQAYDQIDGAPEEKARGITISTAHVEYETENRHYAHVDCPGH
ADYVKNMITGAAQMDGAILVVNAADGPMPQTREHILLGRQVGIPTMVVFMNKVDQVDDEELLELVEMEIRELLSSYDYPG
DDIPVIPGSALAAMEGRDPEIGEEAIKKLMAAVDEFIPTPERAIDQPFLMPVEDVFSISGRGTVVTGRVERGVINVGDNI
EIVGIKDTQSTTCTGVEMFRKLLDRGEAGDNIGALLRGIDREAVERGQVLCKPGSVKPHTKFEAEAYILTKDEGGRHTPF
FANYRPQFYFRTTDVTGTVQLAEGTEMVMPGDNVSFGVELIAPIAMEQGLRFAIREGGRTVGAGVVSKITE

Sequences:

>Translated_391_residues
MAKAKFERNKPHVNIGTIGHVDHGKTTLTAAITKYFGDFQAYDQIDGAPEEKARGITISTAHVEYETENRHYAHVDCPGH
ADYVKNMITGAAQMDGAILVVNAADGPMPQTREHILLGRQVGIPTMVVFMNKVDQVDDEELLELVEMEIRELLSSYDYPG
DDIPVIPGSALAAMEGRDPEIGEEAIKKLMAAVDEFIPTPERAIDQPFLMPVEDVFSISGRGTVVTGRVERGVINVGDNI
EIVGIKDTQSTTCTGVEMFRKLLDRGEAGDNIGALLRGIDREAVERGQVLCKPGSVKPHTKFEAEAYILTKDEGGRHTPF
FANYRPQFYFRTTDVTGTVQLAEGTEMVMPGDNVSFGVELIAPIAMEQGLRFAIREGGRTVGAGVVSKITE
>Mature_390_residues
AKAKFERNKPHVNIGTIGHVDHGKTTLTAAITKYFGDFQAYDQIDGAPEEKARGITISTAHVEYETENRHYAHVDCPGHA
DYVKNMITGAAQMDGAILVVNAADGPMPQTREHILLGRQVGIPTMVVFMNKVDQVDDEELLELVEMEIRELLSSYDYPGD
DIPVIPGSALAAMEGRDPEIGEEAIKKLMAAVDEFIPTPERAIDQPFLMPVEDVFSISGRGTVVTGRVERGVINVGDNIE
IVGIKDTQSTTCTGVEMFRKLLDRGEAGDNIGALLRGIDREAVERGQVLCKPGSVKPHTKFEAEAYILTKDEGGRHTPFF
ANYRPQFYFRTTDVTGTVQLAEGTEMVMPGDNVSFGVELIAPIAMEQGLRFAIREGGRTVGAGVVSKITE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=391, Percent_Identity=57.2890025575448, Blast_Score=484, Evalue=1e-137,
Organism=Homo sapiens, GI223555963, Length=441, Percent_Identity=30.6122448979592, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI5729864, Length=441, Percent_Identity=30.6122448979592, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI4503471, Length=445, Percent_Identity=27.6404494382022, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI4503475, Length=442, Percent_Identity=26.4705882352941, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI54607086, Length=317, Percent_Identity=29.6529968454259, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI194018520, Length=333, Percent_Identity=29.1291291291291, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI194097354, Length=333, Percent_Identity=29.1291291291291, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI194018522, Length=334, Percent_Identity=29.0419161676647, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI46094014, Length=331, Percent_Identity=29.3051359516616, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI4503507, Length=260, Percent_Identity=26.1538461538462, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI157426893, Length=290, Percent_Identity=26.551724137931, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI94966754, Length=144, Percent_Identity=31.25, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=75.8883248730965, Blast_Score=588, Evalue=1e-169,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=75.8883248730965, Blast_Score=588, Evalue=1e-169,
Organism=Escherichia coli, GI2367247, Length=376, Percent_Identity=29.7872340425532, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI48994988, Length=237, Percent_Identity=32.0675105485232, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789108, Length=261, Percent_Identity=31.8007662835249, Blast_Score=92, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17556456, Length=398, Percent_Identity=54.0201005025126, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=43.1524547803618, Blast_Score=322, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI17552884, Length=444, Percent_Identity=28.6036036036036, Blast_Score=151, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17569207, Length=444, Percent_Identity=28.6036036036036, Blast_Score=151, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI32566629, Length=437, Percent_Identity=26.5446224256293, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32566303, Length=431, Percent_Identity=27.8422273781903, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI115532067, Length=441, Percent_Identity=26.3038548752834, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=441, Percent_Identity=26.3038548752834, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=343, Percent_Identity=26.8221574344023, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=166, Percent_Identity=33.1325301204819, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17509919, Length=300, Percent_Identity=27.6666666666667, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=231, Percent_Identity=25.5411255411255, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=65.8291457286432, Blast_Score=527, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6325337, Length=440, Percent_Identity=27.9545454545455, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6319594, Length=440, Percent_Identity=27.9545454545455, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6322937, Length=457, Percent_Identity=24.945295404814, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6320377, Length=341, Percent_Identity=27.8592375366569, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=265, Percent_Identity=26.4150943396226, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322359, Length=131, Percent_Identity=32.824427480916, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=137, Percent_Identity=32.1167883211679, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=260, Percent_Identity=24.6153846153846, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281363316, Length=390, Percent_Identity=60.5128205128205, Blast_Score=489, Evalue=1e-138,
Organism=Drosophila melanogaster, GI17864358, Length=390, Percent_Identity=60.5128205128205, Blast_Score=489, Evalue=1e-138,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=49.740932642487, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI45553807, Length=449, Percent_Identity=26.5033407572383, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI45553816, Length=449, Percent_Identity=26.5033407572383, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24651721, Length=449, Percent_Identity=26.5033407572383, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI17864154, Length=449, Percent_Identity=26.5033407572383, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24652838, Length=443, Percent_Identity=27.0880361173815, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI17137572, Length=443, Percent_Identity=27.0880361173815, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI45550900, Length=435, Percent_Identity=27.3563218390805, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19922690, Length=300, Percent_Identity=29.6666666666667, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI17137380, Length=339, Percent_Identity=30.3834808259587, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24647048, Length=276, Percent_Identity=27.1739130434783, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24647046, Length=276, Percent_Identity=27.1739130434783, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28572034, Length=173, Percent_Identity=32.9479768786127, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24582462, Length=162, Percent_Identity=30.8641975308642, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 42694; Mature: 42563

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERNKPHVNIGTIGHVDHGKTTLTAAITKYFGDFQAYDQIDGAPEEKARGITIST
CCCCCCCCCCCEEEECEEECCCCCCHHHHHHHHHHHCCHHHHHHCCCCCHHHHCCEEEEE
AHVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVNAADGPMPQTREHILLGRQ
EEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHEEECCC
VGIPTMVVFMNKVDQVDDEELLELVEMEIRELLSSYDYPGDDIPVIPGSALAAMEGRDPE
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCC
IGEEAIKKLMAAVDEFIPTPERAIDQPFLMPVEDVFSISGRGTVVTGRVERGVINVGDNI
HHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHCCCCCCEEEEEEECCCEEECCCCE
EIVGIKDTQSTTCTGVEMFRKLLDRGEAGDNIGALLRGIDREAVERGQVLCKPGSVKPHT
EEEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHCCCEEECCCCCCCCC
KFEAEAYILTKDEGGRHTPFFANYRPQFYFRTTDVTGTVQLAEGTEMVMPGDNVSFGVEL
CCCCEEEEEEECCCCCCCCEEECCCCEEEEEEECCCEEEEEECCCEEEECCCCCCCCHHH
IAPIAMEQGLRFAIREGGRTVGAGVVSKITE
HHHHHHHCCCEEEHHCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKAKFERNKPHVNIGTIGHVDHGKTTLTAAITKYFGDFQAYDQIDGAPEEKARGITIST
CCCCCCCCCCEEEECEEECCCCCCHHHHHHHHHHHCCHHHHHHCCCCCHHHHCCEEEEE
AHVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVNAADGPMPQTREHILLGRQ
EEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHEEECCC
VGIPTMVVFMNKVDQVDDEELLELVEMEIRELLSSYDYPGDDIPVIPGSALAAMEGRDPE
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCC
IGEEAIKKLMAAVDEFIPTPERAIDQPFLMPVEDVFSISGRGTVVTGRVERGVINVGDNI
HHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHCCCCCCEEEEEEECCCEEECCCCE
EIVGIKDTQSTTCTGVEMFRKLLDRGEAGDNIGALLRGIDREAVERGQVLCKPGSVKPHT
EEEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHCCCEEECCCCCCCCC
KFEAEAYILTKDEGGRHTPFFANYRPQFYFRTTDVTGTVQLAEGTEMVMPGDNVSFGVEL
CCCCEEEEEEECCCCCCCCEEECCCCEEEEEEECCCEEEEEECCCEEEECCCCCCCCHHH
IAPIAMEQGLRFAIREGGRTVGAGVVSKITE
HHHHHHHCCCEEEHHCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA