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The map label for this gene is pckA

Identifier: 110678696

GI number: 110678696

Start: 1321479

End: 1323077

Strand: Reverse

Name: pckA

Synonym: RD1_1376

Alternate gene names: 110678696

Gene position: 1323077-1321479 (Counterclockwise)

Preceding gene: 110678704

Following gene: 110678693

Centisome position: 32.01

GC content: 57.29

Gene sequence:

>1599_bases
ATGACACATGGACGGGTAAACCCGGACTTTCGGCTCGAGGATCAAGGGATCACAGGGCTTGGCACTGTCTATTACAACCT
TATTGAGCCCGACCTGATCCAATACGCGCTGGCCCGTGGCGAAGGGTCGCTTGGCAAAGGCGGATCGTTCCTTGTCACCA
CAGGCAAATTCACCGGGCGCTCGCCAAAGGACAAACACGTCGTCAAGACTGACTCGGTTGCCGATACGATCTGGTGGGAA
AACAACAGGGAGATGTCACCCGAGGGGTTCGACCAACTCTACACGGACATGCTTGCCCATATGGAAGGGCGCGATTATTT
TGTTGAAGATCTGACCGGTGGTTCGGACCCCAAACACGCGATCAACGTGCGTATGGTTACCGAACTGGCATGGCATGGGC
TGTTTATCCGCCACATGCTGCGCCGCCCGGACCGCGAAGATCTGGATGATTTCATCGCTGATTTCACCGTTATCAACTGC
CCAAGCTTTCAGGCGGACCCCGCCAAACACAACTGCCGTTCAGAAACCGTCATCGCGATGAATTTCGACAAGAAACTCAT
CCTGATCGGCGGCACGGAATATGCCGGCGAAAACAAGAAGTCAGTCTTCACCCTGCTGAATTACCTGCTGCCTGAAAAAG
GCGTGATGCCGATGCATTGCTCTGCCAACCACGCAGTGGGGAATCCGGTGGATACGGCCGTGTTCTTTGGCCTGTCCGGT
ACGGGCAAAACAACATTGTCCGCCGATCCTGCGCGCACGCTGATCGGGGACGACGAACATGGCTGGTCCGACACCGGCAC
GTTCAACTTTGAGGGCGGCTGCTATGCCAAAACCATCAGCCTGAACCCCGAAGCTGAACCCGAAATCTACGCCACCACAG
AAAAATTCGGCACTGTTATCGAAAACATGGTCTATGATGCGGAAACCAAAGACCTTGATTTTGAAGATGACAGCCTGACA
GCCAACATGCGCTGCGCTTACCCGCTGCACTATATCTCCAACGCTTCGCAAGCGGCGCGGGGCGGTCACCCCAAGAACAT
CATCATGTTGACCTGCGATGCCTTTGGGGTGTTGCCGCCCATCTCACGGCTGACGCCTGCGCAGGCGATGTATCACTTCC
TGTCCGGGTTCACATCCAAGGTCGCCGGGACAGAGCGTGGCGTGACTGAACCGGAACCGACCTTTTCAACCTGTTTCGGC
GCCCCCTTCATGCCCCGCCGCCCGGAGGTCTATGGCAACCTGCTGCGTGAAAAGATCGCGACCCATGGCGCGACCTGCTG
GCTGGTGAACACGGGATGGACAGGTGGCGCCTATGGCACGGGCAGCCGGATGCCAATCAAAGCGACGCGGGGTCTTTTGA
CGGCAGCACTGGATGGTTCTCTGGCGGATGCCGAATTCCGCAAGGATCCCAACTTCGGCTTCCAAGTGCCGGTCGATGTC
ACAGGCGTGCCCGGCATTCTGCTTGATCCGCGCAGGACCTGGGACGATGCAGAAGCCTACGACCGGCAAGCGGCCAAACT
CGTCAAGATGTTCTCGGACAACTTCGAACAATACCTGCCGTTCATTGACGAAGACGTCCGCGCGGCCGCCATAAGCTGA

Upstream 100 bases:

>100_bases
ACTTCGTAAAACGTCATGTTATGGACCTGCAGGCGGCCCACGCCGTCCGACGTAAATTGCGACAATCCGCCATCAAAGCG
GGCATCTAGGAGAAGTAAAC

Downstream 100 bases:

>100_bases
CCGCGGCAGTCGTTCGTACAGATCCTTCGAATGCCTGATTGCGGGGCTTATGCAGCCAATGCGTCAAGCTCCGCAGCAGG
CAGATCGAGGATGAAACGAA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MTHGRVNPDFRLEDQGITGLGTVYYNLIEPDLIQYALARGEGSLGKGGSFLVTTGKFTGRSPKDKHVVKTDSVADTIWWE
NNREMSPEGFDQLYTDMLAHMEGRDYFVEDLTGGSDPKHAINVRMVTELAWHGLFIRHMLRRPDREDLDDFIADFTVINC
PSFQADPAKHNCRSETVIAMNFDKKLILIGGTEYAGENKKSVFTLLNYLLPEKGVMPMHCSANHAVGNPVDTAVFFGLSG
TGKTTLSADPARTLIGDDEHGWSDTGTFNFEGGCYAKTISLNPEAEPEIYATTEKFGTVIENMVYDAETKDLDFEDDSLT
ANMRCAYPLHYISNASQAARGGHPKNIIMLTCDAFGVLPPISRLTPAQAMYHFLSGFTSKVAGTERGVTEPEPTFSTCFG
APFMPRRPEVYGNLLREKIATHGATCWLVNTGWTGGAYGTGSRMPIKATRGLLTAALDGSLADAEFRKDPNFGFQVPVDV
TGVPGILLDPRRTWDDAEAYDRQAAKLVKMFSDNFEQYLPFIDEDVRAAAIS

Sequences:

>Translated_532_residues
MTHGRVNPDFRLEDQGITGLGTVYYNLIEPDLIQYALARGEGSLGKGGSFLVTTGKFTGRSPKDKHVVKTDSVADTIWWE
NNREMSPEGFDQLYTDMLAHMEGRDYFVEDLTGGSDPKHAINVRMVTELAWHGLFIRHMLRRPDREDLDDFIADFTVINC
PSFQADPAKHNCRSETVIAMNFDKKLILIGGTEYAGENKKSVFTLLNYLLPEKGVMPMHCSANHAVGNPVDTAVFFGLSG
TGKTTLSADPARTLIGDDEHGWSDTGTFNFEGGCYAKTISLNPEAEPEIYATTEKFGTVIENMVYDAETKDLDFEDDSLT
ANMRCAYPLHYISNASQAARGGHPKNIIMLTCDAFGVLPPISRLTPAQAMYHFLSGFTSKVAGTERGVTEPEPTFSTCFG
APFMPRRPEVYGNLLREKIATHGATCWLVNTGWTGGAYGTGSRMPIKATRGLLTAALDGSLADAEFRKDPNFGFQVPVDV
TGVPGILLDPRRTWDDAEAYDRQAAKLVKMFSDNFEQYLPFIDEDVRAAAIS
>Mature_531_residues
THGRVNPDFRLEDQGITGLGTVYYNLIEPDLIQYALARGEGSLGKGGSFLVTTGKFTGRSPKDKHVVKTDSVADTIWWEN
NREMSPEGFDQLYTDMLAHMEGRDYFVEDLTGGSDPKHAINVRMVTELAWHGLFIRHMLRRPDREDLDDFIADFTVINCP
SFQADPAKHNCRSETVIAMNFDKKLILIGGTEYAGENKKSVFTLLNYLLPEKGVMPMHCSANHAVGNPVDTAVFFGLSGT
GKTTLSADPARTLIGDDEHGWSDTGTFNFEGGCYAKTISLNPEAEPEIYATTEKFGTVIENMVYDAETKDLDFEDDSLTA
NMRCAYPLHYISNASQAARGGHPKNIIMLTCDAFGVLPPISRLTPAQAMYHFLSGFTSKVAGTERGVTEPEPTFSTCFGA
PFMPRRPEVYGNLLREKIATHGATCWLVNTGWTGGAYGTGSRMPIKATRGLLTAALDGSLADAEFRKDPNFGFQVPVDVT
GVPGILLDPRRTWDDAEAYDRQAAKLVKMFSDNFEQYLPFIDEDVRAAAIS

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=523, Percent_Identity=46.4627151051625, Blast_Score=456, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6322950, Length=532, Percent_Identity=45.6766917293233, Blast_Score=455, Evalue=1e-129,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_ROSDO (Q16AH6)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_681703.1
- ProteinModelPortal:   Q16AH6
- SMR:   Q16AH6
- STRING:   Q16AH6
- GeneID:   4197310
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_1376
- NMPDR:   fig|375451.6.peg.1289
- eggNOG:   COG1866
- HOGENOM:   HBG293387
- OMA:   FAQDLYG
- PhylomeDB:   Q16AH6
- ProtClustDB:   PRK09344
- BioCyc:   RDEN375451:RD1_1376-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 58649; Mature: 58517

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHGRVNPDFRLEDQGITGLGTVYYNLIEPDLIQYALARGEGSLGKGGSFLVTTGKFTGR
CCCCCCCCCCEECCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCC
SPKDKHVVKTDSVADTIWWENNREMSPEGFDQLYTDMLAHMEGRDYFVEDLTGGSDPKHA
CCCCCCEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEHHHCCCCCCCCCE
INVRMVTELAWHGLFIRHMLRRPDREDLDDFIADFTVINCPSFQADPAKHNCRSETVIAM
EEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCEEEECCCCCCCCCCCCCCCCEEEEE
NFDKKLILIGGTEYAGENKKSVFTLLNYLLPEKGVMPMHCSANHAVGNPVDTAVFFGLSG
ECCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCEEEEEEECC
TGKTTLSADPARTLIGDDEHGWSDTGTFNFEGGCYAKTISLNPEAEPEIYATTEKFGTVI
CCCCEECCCCCHHHCCCCCCCCCCCCEEEECCCEEEEEEECCCCCCCCEEEEHHHHHHHH
ENMVYDAETKDLDFEDDSLTANMRCAYPLHYISNASQAARGGHPKNIIMLTCDAFGVLPP
HHHHHCCCCCCCCCCCCCCEECEEEECCHHHHCCCHHHHCCCCCCEEEEEEECCCCCCCC
ISRLTPAQAMYHFLSGFTSKVAGTERGVTEPEPTFSTCFGAPFMPRRPEVYGNLLREKIA
HHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHH
THGATCWLVNTGWTGGAYGTGSRMPIKATRGLLTAALDGSLADAEFRKDPNFGFQVPVDV
HCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEEEEC
TGVPGILLDPRRTWDDAEAYDRQAAKLVKMFSDNFEQYLPFIDEDVRAAAIS
CCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCHHHCCC
>Mature Secondary Structure 
THGRVNPDFRLEDQGITGLGTVYYNLIEPDLIQYALARGEGSLGKGGSFLVTTGKFTGR
CCCCCCCCCEECCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCC
SPKDKHVVKTDSVADTIWWENNREMSPEGFDQLYTDMLAHMEGRDYFVEDLTGGSDPKHA
CCCCCCEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEHHHCCCCCCCCCE
INVRMVTELAWHGLFIRHMLRRPDREDLDDFIADFTVINCPSFQADPAKHNCRSETVIAM
EEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCEEEECCCCCCCCCCCCCCCCEEEEE
NFDKKLILIGGTEYAGENKKSVFTLLNYLLPEKGVMPMHCSANHAVGNPVDTAVFFGLSG
ECCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCEEEEEEECC
TGKTTLSADPARTLIGDDEHGWSDTGTFNFEGGCYAKTISLNPEAEPEIYATTEKFGTVI
CCCCEECCCCCHHHCCCCCCCCCCCCEEEECCCEEEEEEECCCCCCCCEEEEHHHHHHHH
ENMVYDAETKDLDFEDDSLTANMRCAYPLHYISNASQAARGGHPKNIIMLTCDAFGVLPP
HHHHHCCCCCCCCCCCCCCEECEEEECCHHHHCCCHHHHCCCCCCEEEEEEECCCCCCCC
ISRLTPAQAMYHFLSGFTSKVAGTERGVTEPEPTFSTCFGAPFMPRRPEVYGNLLREKIA
HHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHH
THGATCWLVNTGWTGGAYGTGSRMPIKATRGLLTAALDGSLADAEFRKDPNFGFQVPVDV
HCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEEEEC
TGVPGILLDPRRTWDDAEAYDRQAAKLVKMFSDNFEQYLPFIDEDVRAAAIS
CCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA