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The map label for this gene is trmD

Identifier: 110678631

GI number: 110678631

Start: 1264821

End: 1265546

Strand: Reverse

Name: trmD

Synonym: RD1_1307

Alternate gene names: 110678631

Gene position: 1265546-1264821 (Counterclockwise)

Preceding gene: 110678632

Following gene: 110678630

Centisome position: 30.62

GC content: 63.36

Gene sequence:

>726_bases
ATGGCAGCGGACGAAGAGCTGTCAGGCGTCTGGCAAGCCCGGGTAGTCACCTTGTTCCCGGACGCCTTTCCCGGTGTCCT
TGGCCTGTCGCTGACGGGCAAGGCGCTACAGGACGGGCGCTGGCAGTTGCACACCCATGACCTGCGCAGCTTTGGCGTGG
GCAAGCATCGTAACGTGGACGACACGCCCGCAGGCGGCGGGGCGGGCATGGTCATGCGCGCAGATGTCGTCGGCCCGGCG
ATTGAGGCGGCACAACGCTTTGCCCGGGGGCGCTGGCCGATCCTCTACATGTCGCCCCGGGGCCGTCCGTTTACACAGAA
AATGGCCAAGGATCTCGCGCGCTGTGACGGTGTAACCGTGTTATGTGGGCGCTTTGAAGGGGTCGATGAACGTGTTCTGG
AACACTTTGGCGTCACCGAGGTGTCCATCGGCGACTATGTTCTGACAGGTGGCGAGATTGCCGCACAAACGGTGCTGGAT
GCCACCGTTCGGCTTTTGCCCGGTGTTCTGGGCAACGCGGAAAGCACCGTCGAGGAAAGCCATTCCAATGGCCTGCTGGA
GCATCCGCAGTATACCCGCCCGGCCATCTGGGAAGGGCACGCCATTCCAGAGGTGCTCATGTCCGGCAATCATGCTGAGA
TCGCGAAATGGCGCGCGGCTCAATCACACCGCCTGACGCAGAGCCGTCGTCCGGACCTGTGGGCCGCCAAGGGGGCCAAG
GAATAA

Upstream 100 bases:

>100_bases
CTCATTGCCGATCCGCCAGAGGGGGCGCTCTGAGGTGGTGCAGGCACCAAAAAGCCACGGCCGCAAGACCATCCGCCCGA
CGCGCACCCCAAGCGCGCTG

Downstream 100 bases:

>100_bases
CCCACCGGACGCGACGCTGCCCCCGCGCCGAAGGAGAGATAGTGGACAATCGCCCGGCAACTGCCGGGATTATCCGTGAA
ATCACCTAAGCCCTTGATGC

Product: tRNA (guanine-N1)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MAADEELSGVWQARVVTLFPDAFPGVLGLSLTGKALQDGRWQLHTHDLRSFGVGKHRNVDDTPAGGGAGMVMRADVVGPA
IEAAQRFARGRWPILYMSPRGRPFTQKMAKDLARCDGVTVLCGRFEGVDERVLEHFGVTEVSIGDYVLTGGEIAAQTVLD
ATVRLLPGVLGNAESTVEESHSNGLLEHPQYTRPAIWEGHAIPEVLMSGNHAEIAKWRAAQSHRLTQSRRPDLWAAKGAK
E

Sequences:

>Translated_241_residues
MAADEELSGVWQARVVTLFPDAFPGVLGLSLTGKALQDGRWQLHTHDLRSFGVGKHRNVDDTPAGGGAGMVMRADVVGPA
IEAAQRFARGRWPILYMSPRGRPFTQKMAKDLARCDGVTVLCGRFEGVDERVLEHFGVTEVSIGDYVLTGGEIAAQTVLD
ATVRLLPGVLGNAESTVEESHSNGLLEHPQYTRPAIWEGHAIPEVLMSGNHAEIAKWRAAQSHRLTQSRRPDLWAAKGAK
E
>Mature_240_residues
AADEELSGVWQARVVTLFPDAFPGVLGLSLTGKALQDGRWQLHTHDLRSFGVGKHRNVDDTPAGGGAGMVMRADVVGPAI
EAAQRFARGRWPILYMSPRGRPFTQKMAKDLARCDGVTVLCGRFEGVDERVLEHFGVTEVSIGDYVLTGGEIAAQTVLDA
TVRLLPGVLGNAESTVEESHSNGLLEHPQYTRPAIWEGHAIPEVLMSGNHAEIAKWRAAQSHRLTQSRRPDLWAAKGAKE

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=219, Percent_Identity=43.8356164383562, Blast_Score=184, Evalue=4e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_ROSDO (Q16AP1)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_681638.1
- ProteinModelPortal:   Q16AP1
- SMR:   Q16AP1
- STRING:   Q16AP1
- GeneID:   4197401
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_1307
- NMPDR:   fig|375451.6.peg.1224
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- PhylomeDB:   Q16AP1
- BioCyc:   RDEN375451:RD1_1307-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 26180; Mature: 26049

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: BINDING 123-123

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAADEELSGVWQARVVTLFPDAFPGVLGLSLTGKALQDGRWQLHTHDLRSFGVGKHRNVD
CCCCHHHHHHHHHEEEEECCCCCCHHEECCCCCCHHHCCCEEEEHHHHHHCCCCCCCCCC
DTPAGGGAGMVMRADVVGPAIEAAQRFARGRWPILYMSPRGRPFTQKMAKDLARCDGVTV
CCCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEE
LCGRFEGVDERVLEHFGVTEVSIGDYVLTGGEIAAQTVLDATVRLLPGVLGNAESTVEES
EECCCCCHHHHHHHHCCCCEEECCCEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
HSNGLLEHPQYTRPAIWEGHAIPEVLMSGNHAEIAKWRAAQSHRLTQSRRPDLWAAKGAK
HCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
E
C
>Mature Secondary Structure 
AADEELSGVWQARVVTLFPDAFPGVLGLSLTGKALQDGRWQLHTHDLRSFGVGKHRNVD
CCCHHHHHHHHHEEEEECCCCCCHHEECCCCCCHHHCCCEEEEHHHHHHCCCCCCCCCC
DTPAGGGAGMVMRADVVGPAIEAAQRFARGRWPILYMSPRGRPFTQKMAKDLARCDGVTV
CCCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEE
LCGRFEGVDERVLEHFGVTEVSIGDYVLTGGEIAAQTVLDATVRLLPGVLGNAESTVEES
EECCCCCHHHHHHHHCCCCEEECCCEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
HSNGLLEHPQYTRPAIWEGHAIPEVLMSGNHAEIAKWRAAQSHRLTQSRRPDLWAAKGAK
HCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA