Click here to switch to the map view.

The map label for this gene is gcvH [H]

Identifier: 110678551

GI number: 110678551

Start: 1189035

End: 1189394

Strand: Direct

Name: gcvH [H]

Synonym: RD1_1223

Alternate gene names: 110678551

Gene position: 1189035-1189394 (Clockwise)

Preceding gene: 110678550

Following gene: 110678552

Centisome position: 28.77

GC content: 55.56

Gene sequence:

>360_bases
ATGAAATTCACAGAAGAGCACGAATGGTTGCGCCCGGACGGTGACGAAATCATCGTGGGCATCACGACACATGCCGCCGA
ACAGTTGGGCGATGTCGTGTTTCTGGAACTGCCCGAAGAGGGCACAACCGTCAGCAAGGATGATGAGGTCGTGGTGATCG
AAAGTGTCAAGGCGGCCAGTGATATTCTGGCCCCCATTGATGGCGAGATTACCGAGGTGAACACGGCACTTGCCGACAAT
CCGGGCGCGGTCAACGAGGACCCGCAGGGAGAAGCCTGGTTTTTCAAGATGAAACCCAGCGATCCATCCCAGATGGACGA
TTTCATGGATGAAGCCGGCTATCAGGCGTTTATCGCCTGA

Upstream 100 bases:

>100_bases
CGCTCTGGGGTGAGGTGCGCAGCAAGCGCATGGGGCTGAAGGTCGTTGACCTGCCGTTCGTTCCCGCAGGATTTAAACGT
TAGAAAACAGGAGACAGGCC

Downstream 100 bases:

>100_bases
CCCGCTGCGTCCGATGCGCCTGACCCCCGCCAGCACAGAGAGAATGATCCGATGAGTTTCAAGCCGATAGACTATCTGCC
CTATGATTTTGCCAACCGGC

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 119; Mature: 119

Protein sequence:

>119_residues
MKFTEEHEWLRPDGDEIIVGITTHAAEQLGDVVFLELPEEGTTVSKDDEVVVIESVKAASDILAPIDGEITEVNTALADN
PGAVNEDPQGEAWFFKMKPSDPSQMDDFMDEAGYQAFIA

Sequences:

>Translated_119_residues
MKFTEEHEWLRPDGDEIIVGITTHAAEQLGDVVFLELPEEGTTVSKDDEVVVIESVKAASDILAPIDGEITEVNTALADN
PGAVNEDPQGEAWFFKMKPSDPSQMDDFMDEAGYQAFIA
>Mature_119_residues
MKFTEEHEWLRPDGDEIIVGITTHAAEQLGDVVFLELPEEGTTVSKDDEVVVIESVKAASDILAPIDGEITEVNTALADN
PGAVNEDPQGEAWFFKMKPSDPSQMDDFMDEAGYQAFIA

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=117, Percent_Identity=48.7179487179487, Blast_Score=124, Evalue=2e-29,
Organism=Homo sapiens, GI89057342, Length=117, Percent_Identity=48.7179487179487, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1789271, Length=119, Percent_Identity=52.1008403361345, Blast_Score=129, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17551294, Length=115, Percent_Identity=44.3478260869565, Blast_Score=110, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17507493, Length=115, Percent_Identity=44.3478260869565, Blast_Score=109, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6319272, Length=99, Percent_Identity=48.4848484848485, Blast_Score=104, Evalue=4e-24,
Organism=Drosophila melanogaster, GI17865652, Length=119, Percent_Identity=41.1764705882353, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13028; Mature: 13028

Theoretical pI: Translated: 3.64; Mature: 3.64

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTEEHEWLRPDGDEIIVGITTHAAEQLGDVVFLELPEEGTTVSKDDEVVVIESVKAAS
CCCCCCCCCCCCCCCEEEEEEEHHHHHHHCCEEEEEECCCCCEECCCCCEEEEECCCCHH
DILAPIDGEITEVNTALADNPGAVNEDPQGEAWFFKMKPSDPSQMDDFMDEAGYQAFIA
HHCCCCCCCEEEEEHEECCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEECC
>Mature Secondary Structure
MKFTEEHEWLRPDGDEIIVGITTHAAEQLGDVVFLELPEEGTTVSKDDEVVVIESVKAAS
CCCCCCCCCCCCCCCEEEEEEEHHHHHHHCCEEEEEECCCCCEECCCCCEEEEECCCCHH
DILAPIDGEITEVNTALADNPGAVNEDPQGEAWFFKMKPSDPSQMDDFMDEAGYQAFIA
HHCCCCCCCEEEEEHEECCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]