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The map label for this gene is nadE [H]

Identifier: 110678543

GI number: 110678543

Start: 1180567

End: 1182225

Strand: Direct

Name: nadE [H]

Synonym: RD1_1215

Alternate gene names: 110678543

Gene position: 1180567-1182225 (Clockwise)

Preceding gene: 110678541

Following gene: 110678544

Centisome position: 28.56

GC content: 60.16

Gene sequence:

>1659_bases
ATGGCGGATCGTTTTCGAATTACTCTGGGTCAGATGAACCCGACGGTGGGCGATCTGGCCGGTAACGCCGCACTCGCGCG
CGAGGTCTGGGAAGCGGGCAAAACCGCCGGGGCCGATCTCGTCGCGCTGCCCGAGATGTTCATCACAGGCTATAACGCGC
AGGATCTGGTGATGAAGCCCGCGTTTCAACTGGATGTGGCGCGACACGTCGACAGGCTGGCGCAAGACTGTGCGGACGGG
CCGACGCTGGCCATCGGTGCCCCATGGGTTGAAGGTACGCGGCTGTACAATGCCTACCTGATCCTGAAGGGCGGCAAAAT
CGTCAGTCAGGTGCTCAAGCACCATCTGCCCAATGAGACTGTTTTTGACGAAGTACGCATTTTTGATGCCGGACCGCTTG
GTGGGCCCTATTCCGTGGGCAATTCACGCGTTGGCAGCCCGATCTGCGAAGACGCGTGGCACGAGGATGTGGCGGAAACG
CTGGCAGAAACCGGGGCCGAGTTTTTACTCGTGCCCAATGGATCGCCCTATTATCGCGGGAAATTCGACACCCGCCTGAA
CCACATGGTGGCCCGCGTCATCGAAACCGAATTGCCGCTGATCTACCTCAATATGGTGGGCGGTCAGGACGATCAGGTGT
TTGACGGGGCCAGTTTCGGGCTGAACCCCGGCGGCAATCTGGCGGTGCAACTGCCGGTTTTCGATGAGGCGGTCGCACAT
GTCGATCTTGAACGCGGGCCTCAAGGGTGGCGGATTGTTGAGGGGGAAAAAGCACATCATCCGGATGCCTGGGAGCAGGA
TTACCGCGTGATGGTGCAGGCGCTGCGCGATTATTGCCGCAAGACAGGGTTTGGCAAAGTGCTGCTCGGGCTGTCGGGCG
GGGTCGATTCCGCCCTTGTTGCGGCCATTGCGGTTGATGCCTTGGGGGCGCAGAACGTGCGTTGCGTGATGCTGCCGTCG
GAATACACTTCGGCGGAATCGCTGGAGGATGCCGAGGCCGTCGCGAAGGCGCTCGGGTGTCACTATGATTACGTGCCGAT
CACCGAAGGGCGCGCCGCCATTGAACAGACTTTGGCACCGCTTTTCGAGGGGCGCGGCGCGGATTTGACCGAGGAAAACA
TCCAATCCCGGCTGCGGGGCTTGTTGTTGATGGCCATGTCCAACAAGTTCGGCGAGATGCTCTTGACCACCGGGAATAAA
TCCGAGGTGGCCGTGGGTTATGCGACCATCTATGGCGACATGGCGGGCGGGTATAATCCGATCAAGGACCTCTACAAAAC
CCGCGTGTTTGAGACCTGTCGGTGGCGCAACAAGACCCACCGTGACTGGATGAAAGGCCCGCAGGGCGAGGTGATCACGC
CGCGCGTGATTGACAAACCGCCCTCCGCCGAGCTGCGCGCAGATCAAAAAGACAGTGACAGCCTGCCTGATTACCCGGAA
CTGGACCGGCTGCTTGAGATACTGGTCGACCGGAATGGCTCCATCGAGGATTGTGTCGCCGCAGGCAGTGATGTGGAGAC
GGCCAGGAAGATCGAACACCTGATTTATATCAGTGAATACAAACGGTTCCAGTCCGCACCGGGTGCGCGTCTGACCAAAG
GGGCGTTCTGGCTGGACCGTCGCTATCCGATTGTGAACCGCTGGCGGGACCGGGGCTGA

Upstream 100 bases:

>100_bases
CGCCCTTTTGCCGCAATAGGCGGGGTTGGGCTTTCATCGGCGGCACGATCTGCTACATGGTTCAAAATCAGGCAGGCGGG
CAGGCAAAAGGGCCTTATCC

Downstream 100 bases:

>100_bases
AAGCGGTCCACATCCCGGTTATTGATGATGCGAAACGCAAAACTGTGATTTGCCAAAGGGTATCGCCACCTGTTTTGAAG
GACACATCATCAAGGAATGG

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MADRFRITLGQMNPTVGDLAGNAALAREVWEAGKTAGADLVALPEMFITGYNAQDLVMKPAFQLDVARHVDRLAQDCADG
PTLAIGAPWVEGTRLYNAYLILKGGKIVSQVLKHHLPNETVFDEVRIFDAGPLGGPYSVGNSRVGSPICEDAWHEDVAET
LAETGAEFLLVPNGSPYYRGKFDTRLNHMVARVIETELPLIYLNMVGGQDDQVFDGASFGLNPGGNLAVQLPVFDEAVAH
VDLERGPQGWRIVEGEKAHHPDAWEQDYRVMVQALRDYCRKTGFGKVLLGLSGGVDSALVAAIAVDALGAQNVRCVMLPS
EYTSAESLEDAEAVAKALGCHYDYVPITEGRAAIEQTLAPLFEGRGADLTEENIQSRLRGLLLMAMSNKFGEMLLTTGNK
SEVAVGYATIYGDMAGGYNPIKDLYKTRVFETCRWRNKTHRDWMKGPQGEVITPRVIDKPPSAELRADQKDSDSLPDYPE
LDRLLEILVDRNGSIEDCVAAGSDVETARKIEHLIYISEYKRFQSAPGARLTKGAFWLDRRYPIVNRWRDRG

Sequences:

>Translated_552_residues
MADRFRITLGQMNPTVGDLAGNAALAREVWEAGKTAGADLVALPEMFITGYNAQDLVMKPAFQLDVARHVDRLAQDCADG
PTLAIGAPWVEGTRLYNAYLILKGGKIVSQVLKHHLPNETVFDEVRIFDAGPLGGPYSVGNSRVGSPICEDAWHEDVAET
LAETGAEFLLVPNGSPYYRGKFDTRLNHMVARVIETELPLIYLNMVGGQDDQVFDGASFGLNPGGNLAVQLPVFDEAVAH
VDLERGPQGWRIVEGEKAHHPDAWEQDYRVMVQALRDYCRKTGFGKVLLGLSGGVDSALVAAIAVDALGAQNVRCVMLPS
EYTSAESLEDAEAVAKALGCHYDYVPITEGRAAIEQTLAPLFEGRGADLTEENIQSRLRGLLLMAMSNKFGEMLLTTGNK
SEVAVGYATIYGDMAGGYNPIKDLYKTRVFETCRWRNKTHRDWMKGPQGEVITPRVIDKPPSAELRADQKDSDSLPDYPE
LDRLLEILVDRNGSIEDCVAAGSDVETARKIEHLIYISEYKRFQSAPGARLTKGAFWLDRRYPIVNRWRDRG
>Mature_551_residues
ADRFRITLGQMNPTVGDLAGNAALAREVWEAGKTAGADLVALPEMFITGYNAQDLVMKPAFQLDVARHVDRLAQDCADGP
TLAIGAPWVEGTRLYNAYLILKGGKIVSQVLKHHLPNETVFDEVRIFDAGPLGGPYSVGNSRVGSPICEDAWHEDVAETL
AETGAEFLLVPNGSPYYRGKFDTRLNHMVARVIETELPLIYLNMVGGQDDQVFDGASFGLNPGGNLAVQLPVFDEAVAHV
DLERGPQGWRIVEGEKAHHPDAWEQDYRVMVQALRDYCRKTGFGKVLLGLSGGVDSALVAAIAVDALGAQNVRCVMLPSE
YTSAESLEDAEAVAKALGCHYDYVPITEGRAAIEQTLAPLFEGRGADLTEENIQSRLRGLLLMAMSNKFGEMLLTTGNKS
EVAVGYATIYGDMAGGYNPIKDLYKTRVFETCRWRNKTHRDWMKGPQGEVITPRVIDKPPSAELRADQKDSDSLPDYPEL
DRLLEILVDRNGSIEDCVAAGSDVETARKIEHLIYISEYKRFQSAPGARLTKGAFWLDRRYPIVNRWRDRG

Specific function: It is not known if this NAD synthase uses NH(3) or glutamine as the N donor [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 60759; Mature: 60627

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRFRITLGQMNPTVGDLAGNAALAREVWEAGKTAGADLVALPEMFITGYNAQDLVMKP
CCCCEEEEECCCCCCHHHHCCCHHHHHHHHHCCCCCCCCCEECCHHHHCCCCCHHHHHCC
AFQLDVARHVDRLAQDCADGPTLAIGAPWVEGTRLYNAYLILKGGKIVSQVLKHHLPNET
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEEEECCHHHHHHHHHHCCCCC
VFDEVRIFDAGPLGGPYSVGNSRVGSPICEDAWHEDVAETLAETGAEFLLVPNGSPYYRG
HHHHEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
KFDTRLNHMVARVIETELPLIYLNMVGGQDDQVFDGASFGLNPGGNLAVQLPVFDEAVAH
CHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEECCCCCCCCCCCCEEEEECCHHHHHHH
VDLERGPQGWRIVEGEKAHHPDAWEQDYRVMVQALRDYCRKTGFGKVLLGLSGGVDSALV
EECCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHH
AAIAVDALGAQNVRCVMLPSEYTSAESLEDAEAVAKALGCHYDYVPITEGRAAIEQTLAP
HHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHH
LFEGRGADLTEENIQSRLRGLLLMAMSNKFGEMLLTTGNKSEVAVGYATIYGDMAGGYNP
HHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEEEEECCCCCCCH
IKDLYKTRVFETCRWRNKTHRDWMKGPQGEVITPRVIDKPPSAELRADQKDSDSLPDYPE
HHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEECCHHCCCCCCCCCCCCCCCCCCCCCCHH
LDRLLEILVDRNGSIEDCVAAGSDVETARKIEHLIYISEYKRFQSAPGARLTKGAFWLDR
HHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCEEEECC
RYPIVNRWRDRG
CCCHHHHHCCCC
>Mature Secondary Structure 
ADRFRITLGQMNPTVGDLAGNAALAREVWEAGKTAGADLVALPEMFITGYNAQDLVMKP
CCCEEEEECCCCCCHHHHCCCHHHHHHHHHCCCCCCCCCEECCHHHHCCCCCHHHHHCC
AFQLDVARHVDRLAQDCADGPTLAIGAPWVEGTRLYNAYLILKGGKIVSQVLKHHLPNET
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEEEECCHHHHHHHHHHCCCCC
VFDEVRIFDAGPLGGPYSVGNSRVGSPICEDAWHEDVAETLAETGAEFLLVPNGSPYYRG
HHHHEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
KFDTRLNHMVARVIETELPLIYLNMVGGQDDQVFDGASFGLNPGGNLAVQLPVFDEAVAH
CHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEECCCCCCCCCCCCEEEEECCHHHHHHH
VDLERGPQGWRIVEGEKAHHPDAWEQDYRVMVQALRDYCRKTGFGKVLLGLSGGVDSALV
EECCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHH
AAIAVDALGAQNVRCVMLPSEYTSAESLEDAEAVAKALGCHYDYVPITEGRAAIEQTLAP
HHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHH
LFEGRGADLTEENIQSRLRGLLLMAMSNKFGEMLLTTGNKSEVAVGYATIYGDMAGGYNP
HHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEEEEECCCCCCCH
IKDLYKTRVFETCRWRNKTHRDWMKGPQGEVITPRVIDKPPSAELRADQKDSDSLPDYPE
HHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEECCHHCCCCCCCCCCCCCCCCCCCCCCHH
LDRLLEILVDRNGSIEDCVAAGSDVETARKIEHLIYISEYKRFQSAPGARLTKGAFWLDR
HHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCEEEECC
RYPIVNRWRDRG
CCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8431308; 8195100 [H]