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The map label for this gene is ghrA [H]

Identifier: 110678532

GI number: 110678532

Start: 1167230

End: 1168162

Strand: Reverse

Name: ghrA [H]

Synonym: RD1_1204

Alternate gene names: 110678532

Gene position: 1168162-1167230 (Counterclockwise)

Preceding gene: 110678533

Following gene: 110678525

Centisome position: 28.26

GC content: 57.34

Gene sequence:

>933_bases
ATGACCCTGACCGTTTTGTTCGCCGCCAGCAATGAGGACTGGCACAAGTACGGCCGCGCACTGAGCGATGCCTTTACCCA
GGAAGGTCTCGACGCGGAACTTGTAACGCGGGCGTCACCACAAGATGTGGACTACATCGTTTATGCCCCCACATCGACGC
TGCAGGACTTCTCTCCCTATAAAAACACCAAAGCCGTTCTGAGCCTTTGGGCCGGGGTCGAGCGCATCGTGACAAATGAC
ACGCTCACCTTTCCGCTGATCAAAATGGTTGATCCCGGCATGACCCAATCCATGGTCGAATGGGTCGTCGGCCATGTCAT
GCGGTATCATCTGGGCATGGACAGCCATATCGTGAATCCGCAGCGCGAGTGGGAGTGGCATTCCAACCCGCTGGCACAGG
AAAGGCGGGTCTGCATTCTGGGGCTGGGATCATTAGGAACTGCTGTTGGTGAACAATTGTGCCGCATGAACTTTCGCGTC
AGCGGCTGGAGCAGATCCGCCAAGAACCTCGACGGTATCGAGACCTTTCACGGATCAGACGGCCTGCGCGATGCTTTGGC
GACAGGCGAAATACTGGTTCTGTTGCTGCCGGACACAGCCGAAACAGAAAATACGCTGAACCGTGAAACGCTGGCCCAGT
TACCCAAGGGCGCGTACATCCTCAACCCCGGGCGCGGGCCGCTGATTGAGGATGCCGCGTTGATCGAAGCCCTTGACAGC
GGTCAGGTCGCGCATGCCACCTTGGATGTGTTCCGCACGGAACCATTGCCACAGGATCACCCGTTTTGGGCGCATCCAAA
TGTGACGGTGACACCACATATCGCGGCGGCAACCCGCCCGGTGACTTCCGCAAAAGAAATTGCCCGCAACATCGTACGCG
GTGAAAAAGGTGAGGCTTTTTTGAACCTCGTTGACCGTGCGCGAGGCTATTGA

Upstream 100 bases:

>100_bases
GCTTTGGGGGTTCGGCCATGCTGGTTTTGATGCTTGCCTTCGGGCTGACGCAAAGTGCGCATGTGCACCGCCCCAGATAA
AGGCCAGATAAAGGACCGAC

Downstream 100 bases:

>100_bases
GCGCTACTCAGCGGCCAGCCCGCGCCCTTTGAGCAGCGCATCCACCCCCGGCAGCCGCCCGCGGAAGGCGATGTAAAGCT
CCGCCGCGTCTGAACTGCCA

Product: 2-hydroxyacid dehydrogenase, putative

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MTLTVLFAASNEDWHKYGRALSDAFTQEGLDAELVTRASPQDVDYIVYAPTSTLQDFSPYKNTKAVLSLWAGVERIVTND
TLTFPLIKMVDPGMTQSMVEWVVGHVMRYHLGMDSHIVNPQREWEWHSNPLAQERRVCILGLGSLGTAVGEQLCRMNFRV
SGWSRSAKNLDGIETFHGSDGLRDALATGEILVLLLPDTAETENTLNRETLAQLPKGAYILNPGRGPLIEDAALIEALDS
GQVAHATLDVFRTEPLPQDHPFWAHPNVTVTPHIAAATRPVTSAKEIARNIVRGEKGEAFLNLVDRARGY

Sequences:

>Translated_310_residues
MTLTVLFAASNEDWHKYGRALSDAFTQEGLDAELVTRASPQDVDYIVYAPTSTLQDFSPYKNTKAVLSLWAGVERIVTND
TLTFPLIKMVDPGMTQSMVEWVVGHVMRYHLGMDSHIVNPQREWEWHSNPLAQERRVCILGLGSLGTAVGEQLCRMNFRV
SGWSRSAKNLDGIETFHGSDGLRDALATGEILVLLLPDTAETENTLNRETLAQLPKGAYILNPGRGPLIEDAALIEALDS
GQVAHATLDVFRTEPLPQDHPFWAHPNVTVTPHIAAATRPVTSAKEIARNIVRGEKGEAFLNLVDRARGY
>Mature_309_residues
TLTVLFAASNEDWHKYGRALSDAFTQEGLDAELVTRASPQDVDYIVYAPTSTLQDFSPYKNTKAVLSLWAGVERIVTNDT
LTFPLIKMVDPGMTQSMVEWVVGHVMRYHLGMDSHIVNPQREWEWHSNPLAQERRVCILGLGSLGTAVGEQLCRMNFRVS
GWSRSAKNLDGIETFHGSDGLRDALATGEILVLLLPDTAETENTLNRETLAQLPKGAYILNPGRGPLIEDAALIEALDSG
QVAHATLDVFRTEPLPQDHPFWAHPNVTVTPHIAAATRPVTSAKEIARNIVRGEKGEAFLNLVDRARGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=173, Percent_Identity=31.7919075144509, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI6912396, Length=165, Percent_Identity=29.0909090909091, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI87081824, Length=319, Percent_Identity=35.1097178683386, Blast_Score=184, Evalue=9e-48,
Organism=Escherichia coli, GI87082289, Length=158, Percent_Identity=31.0126582278481, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789279, Length=198, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=172, Percent_Identity=33.1395348837209, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6325144, Length=179, Percent_Identity=30.1675977653631, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6321253, Length=172, Percent_Identity=30.8139534883721, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI28574286, Length=181, Percent_Identity=30.939226519337, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19921140, Length=167, Percent_Identity=28.7425149700599, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24585514, Length=204, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28574282, Length=204, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28574284, Length=213, Percent_Identity=28.6384976525822, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45552429, Length=204, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45551003, Length=213, Percent_Identity=28.6384976525822, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28571528, Length=185, Percent_Identity=25.4054054054054, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 34252; Mature: 34121

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTVLFAASNEDWHKYGRALSDAFTQEGLDAELVTRASPQDVDYIVYAPTSTLQDFSPY
CEEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEECCCHHHHHCCCC
KNTKAVLSLWAGVERIVTNDTLTFPLIKMVDPGMTQSMVEWVVGHVMRYHLGMDSHIVNP
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
QREWEWHSNPLAQERRVCILGLGSLGTAVGEQLCRMNFRVSGWSRSAKNLDGIETFHGSD
CCCCCCCCCCCHHHCCEEEEECCHHHHHHHHHHHHHCCEECCCCCCCCCCCCCHHHCCCC
GLRDALATGEILVLLLPDTAETENTLNRETLAQLPKGAYILNPGRGPLIEDAALIEALDS
HHHHHHCCCCEEEEEECCCCCHHHHCCHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHCC
GQVAHATLDVFRTEPLPQDHPFWAHPNVTVTPHIAAATRPVTSAKEIARNIVRGEKGEAF
CCCHHHHHHHHHCCCCCCCCCEECCCCCEECCCHHHCCCCCHHHHHHHHHHHCCCCCHHH
LNLVDRARGY
HHHHHHHCCC
>Mature Secondary Structure 
TLTVLFAASNEDWHKYGRALSDAFTQEGLDAELVTRASPQDVDYIVYAPTSTLQDFSPY
EEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEECCCHHHHHCCCC
KNTKAVLSLWAGVERIVTNDTLTFPLIKMVDPGMTQSMVEWVVGHVMRYHLGMDSHIVNP
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
QREWEWHSNPLAQERRVCILGLGSLGTAVGEQLCRMNFRVSGWSRSAKNLDGIETFHGSD
CCCCCCCCCCCHHHCCEEEEECCHHHHHHHHHHHHHCCEECCCCCCCCCCCCCHHHCCCC
GLRDALATGEILVLLLPDTAETENTLNRETLAQLPKGAYILNPGRGPLIEDAALIEALDS
HHHHHHCCCCEEEEEECCCCCHHHHCCHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHCC
GQVAHATLDVFRTEPLPQDHPFWAHPNVTVTPHIAAATRPVTSAKEIARNIVRGEKGEAF
CCCHHHHHHHHHCCCCCCCCCEECCCCCEECCCHHHCCCCCHHHHHHHHHHHCCCCCHHH
LNLVDRARGY
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA